Standard name
Human Ortholog
Description Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.72 0.69 0.88 0.95 0.96 0.87 0.77 0.7 0.6 0.69 0.51 0.57 0.92 0.96 0.98 0.73 0.71 0.68 0.19 0.27 0.25 0.4 0.39 0.44
Endoplasmic Reticulum 0.08 0.23 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0.05 0.11 0.36 0.3 0.18 0.21 0.17 0.18
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.1 0.06 0 0 0.13 0.28 0.29 0.51 0.3 0.65 0.47 0 0 0 0 0.05 0.05 0.25 0.19 0.36 0.25 0.23 0.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0.19 0.16 0.14 0 0.12 0.05 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 0 1 1 5 0 0 0 2 0 1 0 3 1 0 8 4 6 7 11 8
Bud 1 0 1 0 0 0 10 8 7 5 8 5 1 1 0 0 0 1 1 0 0 2 1 3
Bud Neck 0 0 1 0 0 3 2 5 1 3 1 1 1 0 0 0 0 0 2 2 0 1 5 3
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 1 1 3 4 2 1 1 1 1 0 1 1 3 10 6 5 1 2 1 2 9 7
Cytoplasm 112 55 70 120 217 146 210 153 132 170 79 88 117 202 223 129 81 69 33 26 31 58 85 106
Endoplasmic Reticulum 12 18 4 0 0 2 2 5 3 9 0 2 9 6 3 9 6 11 65 29 23 30 36 42
Endosome 0 0 0 2 1 1 0 1 0 0 0 0 0 0 0 12 10 10 4 0 1 0 0 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 2 0 1 0 0 0
Mitochondria 41 8 5 4 6 21 76 63 113 74 100 72 2 0 0 6 6 5 45 18 45 36 48 48
Nucleus 0 0 0 0 1 0 1 3 3 3 0 1 1 1 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 0 0
SpindlePole 0 0 1 0 0 0 1 1 1 1 1 1 0 1 0 0 1 0 1 0 1 0 1 1
Vac/Vac Membrane 3 0 1 2 4 2 16 1 8 4 7 4 0 4 1 33 18 14 5 11 6 4 9 10
Unique Cell Count 155 80 80 126 227 167 272 218 221 246 155 154 127 210 227 176 114 102 181 97 127 146 217 241
Labelled Cell Count 173 85 85 129 233 180 323 246 269 270 197 177 132 217 231 207 129 118 181 97 127 146 217 241


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.6 4.9 4.8 5.5 4.3 4.0 4.5 3.7 3.7 3.4 4.0 5.0 5.5 5.7 6.9 6.7 5.9 5.0 5.1 5.8
Std Deviation (1e-4) 0.5 0.7 1.1 1.2 2.0 1.7 1.6 2.3 1.3 0.8 1.0 1.2 1.0 1.1 1.6 2.0 1.9 1.4 1.4 1.3 2.4
Intensity Change (Log2) – – – -0.01 0.19 -0.18 -0.27 -0.1 -0.4 -0.38 -0.51 -0.27 0.05 0.19 0.23 0.5 0.47 0.29 0.03 0.06 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.8 3.4 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2392 0.7957 1.0815 1.2062 0.7245 1.0537 -0.398 0.2218 0.0301 -0.5588 -0.4 -0.1533 0.3335 -0.5639 -0.9767 -0.3287 -0.7432 -0.5942
Actin 0.0082 0.0012 0.0187 0.01 0.0131 0.0049 0.0046 0.0003 0.0012 0.0003 0.0019 0.0013 0.0523 0.0035 0.0422 0.0673 0.0646 0.0085
Bud 0.0021 0.0007 0.0008 0.0005 0.0029 0.0007 0.0005 0.0002 0.0001 0 0.0002 0.0002 0.0007 0.0046 0.0005 0.0001 0.0006 0.0003
Bud Neck 0.0026 0.0002 0.0013 0.0008 0.0008 0.0007 0.0009 0.0002 0.0012 0.0001 0.0176 0.002 0.0015 0.0012 0.0014 0.0005 0.0076 0.001
Bud Periphery 0.0075 0.0027 0.0017 0.0015 0.008 0.0028 0.0005 0.0006 0.0003 0 0.0004 0.0004 0.0011 0.0023 0.0007 0.0002 0.0008 0.0011
Bud Site 0.025 0.004 0.0052 0.0006 0.0076 0.0005 0.0015 0.0017 0.001 0 0.0011 0.0002 0.0123 0.0397 0.0033 0.0004 0.0055 0.0002
Cell Periphery 0.0033 0.0008 0.0009 0.001 0.0004 0.0003 0.0004 0.0004 0.0003 0.0001 0.0002 0.0003 0.0023 0.0011 0.0009 0.001 0.0004 0.0004
Cytoplasm 0.1753 0.2353 0.1835 0.1677 0.2039 0.2088 0.1461 0.1494 0.1358 0.073 0.1276 0.171 0.2052 0.3362 0.3552 0.4787 0.2468 0.3515
Cytoplasmic Foci 0.0242 0.0111 0.0216 0.023 0.0153 0.0194 0.0142 0.0098 0.0119 0.0048 0.0128 0.0144 0.0399 0.0092 0.0243 0.0073 0.1146 0.0179
Eisosomes 0.0005 0.0003 0.0007 0.0003 0.0003 0.0002 0.0004 0.0002 0.0005 0.0002 0.0003 0.0001 0.001 0.0003 0.0005 0.0002 0.0006 0.0003
Endoplasmic Reticulum 0.0203 0.0269 0.0161 0.0132 0.0101 0.0145 0.0238 0.0113 0.0236 0.0564 0.0077 0.023 0.0356 0.0135 0.0404 0.1101 0.0109 0.0293
Endosome 0.0282 0.0066 0.0112 0.026 0.0131 0.0102 0.0064 0.0058 0.0088 0.0053 0.0062 0.0066 0.0335 0.0026 0.0104 0.0088 0.0378 0.0143
Golgi 0.0093 0.0006 0.0056 0.0167 0.0113 0.005 0.0011 0.0003 0.0005 0.0002 0.0006 0.0003 0.0199 0.0004 0.0122 0.0011 0.016 0.0083
Lipid Particles 0.0277 0.0031 0.0055 0.028 0.0035 0.0089 0.0048 0.0023 0.0021 0.0026 0.0028 0.0017 0.0491 0.0015 0.0136 0.0017 0.0161 0.0159
Mitochondria 0.0032 0.0018 0.0026 0.0222 0.0101 0.0078 0.001 0.0009 0.0013 0.0025 0.0005 0.0051 0.0179 0.0004 0.0187 0.0005 0.0015 0.0107
None 0.6273 0.6944 0.6882 0.6573 0.6855 0.6997 0.7754 0.8028 0.7982 0.8365 0.7972 0.7579 0.4732 0.5738 0.4558 0.3039 0.4485 0.4714
Nuclear Periphery 0.0053 0.003 0.0054 0.0017 0.0034 0.0032 0.0031 0.0025 0.0045 0.0124 0.0036 0.0038 0.0097 0.0014 0.0024 0.007 0.0044 0.0299
Nucleolus 0.0011 0.0004 0.0006 0.0002 0.0003 0.0003 0.0026 0.0007 0.0005 0.0001 0.0016 0.0005 0.0012 0.0006 0.0002 0.0001 0.0006 0.0002
Nucleus 0.003 0.0022 0.0031 0.001 0.0014 0.0027 0.0038 0.0043 0.0027 0.0011 0.0052 0.0042 0.005 0.0024 0.0013 0.0034 0.0028 0.0128
Peroxisomes 0.0153 0.0008 0.0121 0.0251 0.0037 0.0023 0.0018 0.0004 0.0006 0.0001 0.0007 0.0005 0.0254 0.0003 0.0114 0.0021 0.0056 0.0188
Punctate Nuclear 0.0036 0.0017 0.0032 0.0017 0.0011 0.0027 0.005 0.0028 0.003 0.0017 0.0108 0.005 0.0073 0.0035 0.0026 0.0012 0.0121 0.002
Vacuole 0.006 0.0018 0.0084 0.0011 0.0031 0.0033 0.0017 0.0028 0.0014 0.0016 0.0009 0.0012 0.0039 0.0015 0.0018 0.0038 0.0017 0.0023
Vacuole Periphery 0.001 0.0002 0.0036 0.0005 0.0009 0.0012 0.0003 0.0003 0.0003 0.0009 0.0002 0.0002 0.0019 0.0001 0.0003 0.0004 0.0005 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3092 17.0932 11.1916 16.6734 19.6873 16.8623 13.9788 19.8987 18.2668 19.1356
Translational Efficiency 0.6229 0.4292 0.6312 0.5682 0.52 0.5455 0.4974 0.4495 0.4721 0.5248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
Localization
Cell Percentages ER (56%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Tda5

Tda5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available