Standard name
Human Ortholog
Description Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.4 0.35 0.41 0.36 0.19 0.25 – 0.21 0.24 0.22 0.23 0.34 0.34 0.16 0.22 0.23 0.16 0.73 0.66 0.43 0.52 0.42 0.41
Bud 0.09 0.08 0.08 0.19 0.09 0.11 0.14 – 0.14 0.13 0.15 0.12 0.08 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0.05 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0.16 0.38 0.49 0.27 0.46 0.21 0.23 – 0.14 0.23 0.08 0.16 0.44 0.65 0.62 0.36 0.42 0.32 0.12 0.13 0.18 0.25 0.28 0.22
Mitochondria 0.81 0.56 0.39 0.57 0.39 0.6 0.58 – 0.71 0.64 0.86 0.67 0.4 0.38 0.34 0.11 0.14 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0
Peroxisomes 0.2 0.14 0.21 0.3 0.28 0.32 0.25 – 0.2 0.21 0.11 0.24 0.32 0.2 0.32 0.33 0.16 0.18 0.08 0.12 0.27 0.12 0.19 0.27
SpindlePole 0 0 0 0 0 0.06 0.07 – 0 0.05 0 0.06 0 0 0 0.16 0.15 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 96 157 107 87 81 57 81 – 61 73 71 77 72 104 46 62 37 28 261 325 164 112 151 223
Bud 31 32 26 40 21 35 45 – 41 39 49 40 18 19 9 6 6 3 11 11 4 3 5 8
Bud Neck 0 1 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 12 0 0 2 1 0 6 – 3 6 7 10 0 0 0 0 0 4 – – – – – –
Cell Periphery 8 8 8 0 2 3 1 – 4 0 4 6 5 3 2 3 3 1 0 0 0 0 0 0
Cytoplasm 3 8 8 6 2 9 5 – 6 2 4 5 11 12 15 19 12 14 0 0 0 0 0 0
Endoplasmic Reticulum 7 5 0 0 4 3 1 – 2 0 4 1 1 3 0 1 4 4 0 0 0 0 1 0
Endosome 6 7 7 5 6 7 12 – 7 7 4 5 3 3 1 7 5 6 6 15 20 9 15 20
Golgi 55 150 150 58 103 63 73 – 41 72 25 53 94 198 177 99 67 55 42 64 68 53 101 119
Mitochondria 276 221 120 122 88 184 188 – 209 195 276 229 85 117 96 29 22 22 5 8 10 8 9 8
Nucleus 1 1 1 0 0 0 0 – 2 0 1 0 0 0 0 0 1 2 0 1 0 0 0 0
Nuclear Periphery 1 1 2 1 1 2 0 – 0 4 1 1 0 0 0 0 1 0 0 0 1 0 1 0
Nucleolus 5 4 3 4 7 12 4 – 7 6 6 6 2 1 1 16 9 14 0 0 0 0 0 0
Peroxisomes 69 56 66 64 63 97 81 – 59 65 35 80 68 60 92 92 26 30 29 58 103 25 68 145
SpindlePole 10 9 9 4 9 19 23 – 11 14 4 19 9 5 11 44 24 27 1 4 2 2 1 3
Vac/Vac Membrane 2 4 9 0 4 2 0 – 1 2 2 4 1 1 1 2 1 6 0 0 1 0 1 2
Unique Cell Count 339 393 308 214 226 307 322 295 307 322 340 213 305 285 276 161 170 360 494 379 218 360 539
Labelled Cell Count 582 664 516 393 392 494 520 454 485 493 536 369 526 451 381 218 216 360 494 379 218 360 539


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 20.6 15.1 15.2 16.3 14.7 14.6 – 13.2 14.0 12.3 13.9 17.6 18.3 17.6 24.3 25.1 23.9 15.7 16.0 16.9
Std Deviation (1e-4) 5.7 7.2 4.3 3.7 4.6 5.0 4.0 – 3.9 3.9 3.6 4.0 4.6 4.8 4.5 7.1 7.8 7.7 5.1 4.9 4.3
Intensity Change (Log2) – – – 0.01 0.12 -0.03 -0.04 – -0.19 -0.1 -0.29 -0.12 0.23 0.28 0.22 0.69 0.73 0.67 0.06 0.08 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.4 0.3 -4.5 -2.6 – -3.9 -3.0 -3.5 -3.4 -0.2 -0.2 -5.2 -3.3 -2.6 -4.2
Bud 3.5 0.3 1.2 2.2 – 2.1 1.7 2.6 1.4 0 -1.0 -2.7 -3.3 -1.9 -2.9
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -1.5 0 – -1.1 0 -1.2 -0.7 -0.2 -1.5 0 -1.3 0 0
Cytoplasm 0.1 0 0.3 -0.9 – -0.5 0 -1.2 -1.0 1.5 0.9 1.7 2.5 2.5 2.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.3 0 1.1 – 0.1 0 -1.0 -0.8 0 0 0 0.2 0 0
Golgi -5.0 -0.7 -7.3 -6.8 – -9.2 -6.5 -11.5 -9.1 -1.0 4.1 3.3 -3.1 -1.5 -3.5
Mitochondria 4.1 0 5.2 4.9 – 7.9 6.1 12.1 7.2 0.2 -0.2 -1.3 -7.9 -5.7 -6.0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.4 0 – 0 0 0 0 0 0 0 3.3 0 4.1
Peroxisomes 2.2 1.7 2.9 1.1 – -0.4 -0.1 -3.6 0.6 2.7 -0.5 3.0 3.2 -1.4 -1.0
SpindlePole -0.8 0.7 1.9 2.4 – 0.6 1.1 -1.5 1.7 0.8 -1.1 0.6 5.5 4.8 5.1
Vacuole 0 -0.9 -2.1 0 – 0 -2.1 -2.2 -1.6 0 0 0 -2.0 0 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.6831 11.7015 11.7603 11.4358 10.7868 11.3371 15.1814 11.7761 12.9436 11.7146 11.823 13.1561 21.1579 14.145 17.1831 16.9252 17.0102 16.1521
Actin 0.5719 0.7841 0.8406 0.6969 0.7873 0.8516 0.6872 0.7486 0.7962 0.6026 0.7483 0.8664 0.7873 0.7181 0.8472 0.8231 0.8025 0.7875
Bud 0.0006 0.0014 0.0022 0.0005 0.0008 0.0002 0.0009 0.002 0.0013 0.0078 0.0042 0.0002 0.0002 0.002 0.0017 0.002 0.0091 0.0002
Bud Neck 0.0088 0.001 0.0022 0.0013 0.0009 0.0006 0.0037 0.0008 0.0011 0.0127 0.0019 0.0008 0.0006 0.0006 0.0013 0.001 0.0005 0.0009
Bud Periphery 0.0008 0.0011 0.0026 0.0004 0.0008 0.0003 0.0009 0.0013 0.0013 0.0095 0.0064 0.0003 0.0002 0.0013 0.0028 0.0019 0.0061 0.0003
Bud Site 0.0064 0.0073 0.008 0.0011 0.0086 0.0006 0.0142 0.0201 0.0046 0.0235 0.0042 0.0004 0.0007 0.0033 0.005 0.0018 0.0091 0.0004
Cell Periphery 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0001 0.0007 0.0005 0 0 0.0001 0.0001 0.0001 0.0004 0.0001
Cytoplasm 0.0042 0.0024 0.0004 0.0003 0.006 0.0001 0.0023 0.0086 0.0009 0.016 0.0008 0 0.0001 0.0003 0.0001 0.0002 0.001 0.0001
Cytoplasmic Foci 0.0481 0.0415 0.0158 0.0444 0.0245 0.0054 0.029 0.0296 0.0307 0.0208 0.0149 0.018 0.0116 0.0322 0.0125 0.01 0.0148 0.0071
Eisosomes 0.001 0.0006 0.0004 0.0004 0.0005 0.0011 0.0012 0.0003 0.0004 0.0006 0.0006 0.0007 0.0008 0.0003 0.0011 0.0004 0.0004 0.0006
Endoplasmic Reticulum 0.0025 0.0001 0.0005 0.0002 0.0014 0.0002 0.0015 0.0001 0.0002 0.0043 0.0024 0 0 0 0.0001 0 0.0001 0.0001
Endosome 0.0326 0.0074 0.0087 0.0239 0.0271 0.003 0.0158 0.0049 0.0069 0.0194 0.0263 0.0016 0.0032 0.0063 0.0026 0.0042 0.0025 0.0037
Golgi 0.0514 0.0411 0.0309 0.0577 0.0519 0.0182 0.0652 0.0737 0.0375 0.059 0.1179 0.0212 0.1012 0.122 0.0435 0.0923 0.0917 0.1189
Lipid Particles 0.0525 0.0064 0.0113 0.015 0.0191 0.0121 0.021 0.0079 0.0138 0.0067 0.0183 0.0083 0.0046 0.0118 0.0059 0.0013 0.0056 0.0115
Mitochondria 0.0163 0.0144 0.0103 0.0318 0.0095 0.0311 0.0459 0.0102 0.0139 0.0695 0.0177 0.0283 0.0201 0.0047 0.0075 0.0074 0.0048 0.0142
None 0.0187 0.0008 0.0001 0.0001 0.0021 0.0014 0.003 0 0.0004 0.0043 0.0006 0 0 0 0.0001 0 0.0002 0
Nuclear Periphery 0.0111 0 0.0005 0.0002 0.0009 0.0003 0.0063 0 0.0006 0.0194 0.0076 0 0 0 0.0001 0 0.0001 0
Nucleolus 0.0022 0.0001 0.0001 0 0.002 0.0001 0.0004 0.0001 0.0002 0.0015 0.0003 0 0 0 0 0 0.0004 0
Nucleus 0.0046 0.0001 0.0002 0 0.0086 0.0001 0.0028 0 0.0004 0.0216 0.0007 0 0 0 0 0 0.0002 0
Peroxisomes 0.1505 0.0888 0.0643 0.124 0.0438 0.0722 0.0943 0.0907 0.0836 0.0832 0.0202 0.0535 0.0693 0.0964 0.0679 0.0538 0.049 0.054
Punctate Nuclear 0.0119 0.0007 0.0003 0.0004 0.0015 0.0007 0.002 0.0004 0.0054 0.0107 0.0027 0.0001 0 0.0002 0.0002 0 0.0002 0
Vacuole 0.002 0.0002 0.0003 0.0005 0.002 0.0001 0.001 0.0004 0.0001 0.0041 0.0018 0.0001 0 0.0001 0.0001 0.0001 0.0009 0.0001
Vacuole Periphery 0.0016 0.0002 0.0003 0.0007 0.0006 0.0005 0.0009 0.0001 0.0001 0.0021 0.0016 0.0001 0 0.0001 0.0001 0.0001 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.258 67.0695 84.1948 82.5586 82.2821 71.6027 79.7961 87.5821 93.1409 87.6638
Translational Efficiency 1.6957 2.0278 1.5466 1.8815 1.8612 2.2923 1.5987 1.717 1.4459 1.6

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
Localization
Cell Percentages actin (67%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Crn1

Crn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available