Standard name
Human Ortholog
Description Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.1 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.09 0.14 0.27 0.17 – 0.15 0.17 0.19 0.12 0.09 0.16 0.16 0.17 0.14 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.07 0.07 0 0.09 0.06 0.37 0.4 – 0.81 0.73 0.74 0.68 0.18 0.05 0.05 0.11 0 – 0 0 0 0 0.05 0.07
Nucleus 0.89 0.84 0.87 0.88 0.75 0.52 0.57 – 0.46 0.31 0.32 0.54 0.8 0.77 0.7 0.72 0.77 – 0.78 0.77 0.7 0.57 0.42 0.36
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.07 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0 0.09 0 0 0.05 0 0 – 0.05 0.05 0 0.05 0 0.05 0.09 0.07 0.07 – 0 0.1 0.15 0.25 0.25 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 2 2 0 1 2 2
Bud 0 0 0 1 0 2 1 – 4 4 5 0 0 0 0 0 0 – 0 4 1 6 4 7
Bud Neck 0 0 2 0 3 0 2 – 0 1 1 0 0 0 0 1 0 – 0 1 0 0 2 5
Bud Site 0 0 0 1 0 0 3 – 2 4 1 4 0 0 0 2 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0
Cytoplasm 7 16 14 8 22 18 31 – 12 21 14 5 25 41 46 33 22 – 2 3 5 2 4 5
Endoplasmic Reticulum 2 1 1 0 1 0 1 – 0 0 0 0 0 1 1 9 6 – 1 0 2 1 3 3
Endosome 1 3 0 1 2 0 0 – 0 0 0 0 1 2 6 4 1 – 5 4 2 1 11 15
Golgi 0 2 0 0 0 0 0 – 0 0 0 0 1 0 0 2 0 – 3 2 0 1 2 1
Mitochondria 15 27 3 8 9 25 74 – 66 88 55 28 50 14 15 21 6 – 4 6 4 2 15 23
Nucleus 198 326 133 77 121 35 105 – 37 38 24 22 222 203 200 142 121 – 187 334 137 117 120 125
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 – 0 1 1 0 2 2
Nucleolus 4 4 0 0 0 0 0 – 1 2 0 1 8 3 9 2 2 – 12 12 1 10 19 21
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 – 0 0 0 0 1 0
SpindlePole 1 4 2 1 8 0 12 – 3 1 2 0 0 1 1 4 1 – 5 8 4 5 14 24
Vac/Vac Membrane 10 34 5 0 8 1 8 – 4 6 3 2 9 12 27 14 11 – 9 44 28 50 73 99
Unique Cell Count 223 389 153 88 161 67 183 81 121 74 41 279 264 285 197 157 241 432 196 206 288 350
Labelled Cell Count 238 418 160 97 174 81 237 129 165 105 62 316 277 308 234 171 241 432 196 206 288 350


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.2 5.4 5.1 5.5 4.4 4.1 – 3.8 3.6 3.3 3.8 4.7 5.2 4.8 6.9 7.4 – 4.4 4.7 4.8
Std Deviation (1e-4) 1.1 0.9 1.3 1.8 1.9 2.0 1.1 – 1.6 1.6 0.8 1.3 1.4 4.9 1.3 1.6 1.9 – 1.2 1.7 1.5
Intensity Change (Log2) – – – -0.09 0.02 -0.31 -0.41 – -0.5 -0.59 -0.74 -0.51 -0.22 -0.06 -0.19 0.34 0.46 – -0.3 -0.22 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0 1.3 3.4 2.1 – 1.3 2.0 2.1 0 -0.1 1.9 2.0 2.1 1.3 –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 1.7 0 0 – 0 0 0 0 0 1.7 0 3.2 0 –
Nucleus 0.1 -2.7 -5.6 -5.9 – -6.7 -9.4 -8.3 -4.7 -1.9 -2.5 -3.9 -3.4 -2.3 –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0.8 0 0.5 – 0 0 0 0 0 0.6 2.4 1.6 1.5 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0247 3.301 3.0408 2.6727 2.8833 3.076 2.1086 1.7969 1.7716 1.3186 1.4115 1.6537 1.5676 1.5246 1.3743 1.0758 1.1203 1.2521
Actin 0.0293 0.0001 0.0164 0.0005 0.0132 0.0121 0.0496 0.0003 0.0286 0 0.0076 0.0088 0.0134 0 0.0022 0.0006 0.0037 0.0028
Bud 0.0019 0 0.0052 0.0001 0.0002 0.0001 0.0035 0.0007 0.001 0 0.0002 0.0007 0.0021 0.0005 0.0003 0.0001 0.0008 0.0001
Bud Neck 0.0016 0.0003 0.0032 0.0003 0.0009 0.0101 0.0008 0.0004 0.0012 0.0001 0.0015 0.002 0.0035 0.0002 0.0008 0.0006 0.0018 0.0021
Bud Periphery 0.0013 0 0.0021 0.0001 0.0006 0.0003 0.0035 0.0003 0.0025 0 0.0005 0.0008 0.0011 0.0001 0.0009 0.0002 0.0013 0.0001
Bud Site 0.0035 0.0002 0.0016 0.0001 0.0006 0.0006 0.0022 0.0008 0.0077 0 0.0006 0.0005 0.0015 0.0003 0.0007 0.0001 0.0045 0.0002
Cell Periphery 0.0003 0 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0007 0 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0003 0
Cytoplasm 0.006 0.0023 0.0033 0.0171 0.0042 0.0014 0.0094 0.0019 0.0011 0.0002 0.0007 0.0096 0.0056 0.0009 0.004 0.0103 0.0021 0.0012
Cytoplasmic Foci 0.007 0.0001 0.0078 0.0003 0.0083 0.005 0.0308 0.0016 0.0007 0 0.0056 0.007 0.0047 0.0009 0.0019 0.0004 0.0035 0.0013
Eisosomes 0.0003 0 0.0002 0 0.0005 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.002 0.0002 0.0017 0.0026 0.0019 0.0005 0.0029 0.0002 0.0007 0 0.0011 0.0014 0.0014 0 0.0025 0.0086 0.0005 0.0007
Endosome 0.0154 0.0003 0.0069 0.0013 0.0635 0.0053 0.0207 0.0004 0.0021 0 0.0097 0.0105 0.0077 0 0.0116 0.0077 0.0042 0.0018
Golgi 0.0034 0 0.0028 0 0.0077 0.0017 0.0107 0 0.0016 0 0.0283 0.0063 0.0026 0 0.0014 0.0005 0.0011 0.0006
Lipid Particles 0.0149 0 0.0082 0.0003 0.0068 0.0039 0.0153 0.0001 0.0006 0 0.0108 0.0061 0.0095 0 0.0021 0.0014 0.0037 0.0016
Mitochondria 0.007 0.0003 0.0082 0.0019 0.0702 0.0005 0.0316 0.0013 0.0108 0.0002 0.0058 0.0063 0.0161 0.0003 0.0048 0.0318 0.0069 0.0006
None 0.0171 0.0006 0.0137 0.0117 0.0151 0.0241 0.0095 0.0009 0.0096 0 0.0003 0.001 0.0055 0.0019 0.0052 0.0012 0.001 0.0014
Nuclear Periphery 0.0149 0.0099 0.0153 0.048 0.0162 0.0056 0.0481 0.0209 0.0295 0.002 0.0447 0.0188 0.0244 0.009 0.031 0.0352 0.0317 0.0064
Nucleolus 0.0233 0.0214 0.0227 0.0325 0.016 0.0468 0.0184 0.0219 0.0273 0.0106 0.0441 0.033 0.0285 0.0159 0.0285 0.0266 0.0215 0.0406
Nucleus 0.822 0.9591 0.8615 0.8694 0.7258 0.8644 0.6995 0.9304 0.8592 0.9859 0.8131 0.8447 0.7455 0.8337 0.7717 0.793 0.7692 0.8162
Peroxisomes 0.0102 0 0.0029 0 0.0027 0.0045 0.0234 0.0001 0.0006 0 0.0035 0.0076 0.0032 0 0.0006 0.0001 0.0033 0.0015
Punctate Nuclear 0.0151 0.0047 0.0142 0.0117 0.0435 0.0111 0.0151 0.0168 0.0124 0.0007 0.0193 0.0326 0.1182 0.1358 0.1262 0.0781 0.136 0.1203
Vacuole 0.0025 0.0003 0.0009 0.0011 0.0011 0.0013 0.002 0.0005 0.0009 0.0001 0.0011 0.0011 0.0029 0.0001 0.0015 0.0019 0.0021 0.0003
Vacuole Periphery 0.0011 0.0001 0.0009 0.0008 0.001 0.0002 0.0023 0.0005 0.0009 0.0001 0.0012 0.0009 0.002 0.0001 0.0019 0.0015 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5738 11.1204 7.7017 11.1015 11.5589 14.505 11.1501 11.1302 13.1383 13.3594
Translational Efficiency 0.7148 0.6536 0.8413 0.5543 0.5698 0.4584 0.6096 0.6627 0.5011 0.455

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Leu3

Leu3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Leu3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available