Standard name | |
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Human Ortholog | |
Description | Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 9 |
Bud | 3 | 0 | 0 | 0 | 2 | 4 | 5 | 3 | 8 | 5 | 16 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 8 | 25 | 27 |
Bud Neck | 0 | 0 | 0 | 1 | 3 | 0 | 4 | 8 | 3 | 3 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 5 | 7 | 3 | 7 | 11 | 2 | 6 | 10 | 0 | 5 | 3 | 15 | 11 | 7 | 0 | 0 | 0 | 2 | 3 | 4 |
Cytoplasm | 190 | 34 | 35 | 362 | 488 | 435 | 651 | 585 | 382 | 405 | 292 | 312 | 330 | 688 | 648 | 269 | 210 | 220 | 178 | 55 | 60 | 234 | 513 | 492 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 0 | 2 | 1 | 2 | 2 | 3 | 2 | 0 | 17 | 8 | 10 | 0 | 0 | 0 | 0 | 3 | 3 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 11 | 5 | 8 | 1 | 0 | 0 | 3 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 16 | 2 | 0 | 0 | 3 | 23 | 88 | 40 | 72 | 50 | 105 | 58 | 0 | 1 | 0 | 1 | 3 | 6 | 11 | 0 | 2 | 9 | 14 | 19 |
Nucleus | 0 | 0 | 0 | 3 | 3 | 8 | 20 | 23 | 15 | 24 | 9 | 20 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 1 | 2 | 4 | 5 | 0 | 0 | 0 | 2 | 2 | 0 | 3 | 0 | 1 | 6 | 16 | 27 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 24 | 40 | 97 | 105 | 66 | 99 | 62 | 110 | 2 | 5 | 4 | 33 | 19 | 21 | 1 | 0 | 7 | 4 | 6 | 2 |
Unique Cell Count | 203 | 35 | 36 | 365 | 515 | 493 | 795 | 724 | 485 | 543 | 416 | 475 | 334 | 697 | 654 | 312 | 237 | 252 | 208 | 60 | 75 | 277 | 609 | 621 |
Labelled Cell Count | 209 | 36 | 36 | 370 | 533 | 520 | 876 | 781 | 562 | 594 | 499 | 535 | 336 | 707 | 659 | 355 | 265 | 281 | 208 | 60 | 75 | 277 | 609 | 621 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.0 | 5.4 | 5.7 | 6.1 | 6.1 | 5.8 | 6.7 | 6.3 | 6.6 | 6.7 | 6.8 | 5.8 | 5.9 | 6.1 | 9.9 | 10.3 | 9.5 | 5.5 | 5.5 | 5.8 |
Std Deviation (1e-4) | 1.2 | 1.5 | 1.4 | 1.9 | 2.4 | 2.4 | 2.3 | 2.4 | 2.8 | 2.6 | 3.4 | 3.1 | 2.1 | 2.0 | 2.0 | 3.0 | 3.9 | 2.8 | 1.9 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.18 | 0.16 | 0.09 | 0.3 | 0.21 | 0.28 | 0.3 | 0.32 | 0.1 | 0.13 | 0.18 | 0.87 | 0.92 | 0.81 | 0.01 | 0.02 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5789 | 2.5412 | 1.4556 | 1.774 | 1.4092 | 2.1179 | 0.3775 | 2.1627 | 1.3914 | 0.7741 | 1.6083 | 2.1864 | 1.1338 | 3.4523 | 2.2198 | 0.9827 | 1.9049 | 2.3902 |
Actin | 0.0413 | 0.0012 | 0.0203 | 0.0003 | 0.0042 | 0.0009 | 0.0472 | 0.0039 | 0.0112 | 0.0008 | 0.016 | 0.0111 | 0.0103 | 0.0002 | 0.0005 | 0.0003 | 0.0046 | 0.0005 |
Bud | 0.0083 | 0.0115 | 0.0033 | 0.0003 | 0.0077 | 0.0101 | 0.0006 | 0.0015 | 0.0033 | 0.001 | 0.0002 | 0.0002 | 0.001 | 0.0003 | 0.0007 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0029 | 0.0003 | 0.006 | 0.0004 | 0.0017 | 0.001 | 0.0008 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0007 |
Bud Periphery | 0.0057 | 0.0086 | 0.0023 | 0.0002 | 0.002 | 0.0045 | 0.0008 | 0.0014 | 0.0032 | 0.0007 | 0.0003 | 0.0001 | 0.0011 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 |
Bud Site | 0.006 | 0.0223 | 0.0035 | 0.0001 | 0.0023 | 0.0011 | 0.0041 | 0.0048 | 0.0024 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0009 | 0.0002 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.3966 | 0.6219 | 0.5357 | 0.6627 | 0.5543 | 0.5758 | 0.3179 | 0.5488 | 0.5201 | 0.5033 | 0.2709 | 0.6662 | 0.6227 | 0.8562 | 0.7221 | 0.687 | 0.6378 | 0.8066 |
Cytoplasmic Foci | 0.016 | 0.0065 | 0.0101 | 0.0049 | 0.007 | 0.0084 | 0.0254 | 0.0034 | 0.0112 | 0.0119 | 0.029 | 0.0078 | 0.0046 | 0.0055 | 0.004 | 0.0081 | 0.0036 | 0.005 |
Eisosomes | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.0018 | 0.0039 | 0.0018 | 0.0094 | 0.0039 | 0.002 | 0.0009 | 0.0014 | 0.0028 | 0.0006 | 0.0013 | 0.0046 | 0.0016 | 0.0016 | 0.0059 | 0.0009 | 0.0023 |
Endosome | 0.0356 | 0.0016 | 0.019 | 0.0019 | 0.0365 | 0.0109 | 0.0175 | 0.0005 | 0.0059 | 0.004 | 0.012 | 0.0065 | 0.0058 | 0.0015 | 0.0009 | 0.0155 | 0.0009 | 0.0015 |
Golgi | 0.0185 | 0.0003 | 0.0043 | 0.0001 | 0.0074 | 0.0086 | 0.0072 | 0.0001 | 0.0031 | 0.0003 | 0.0375 | 0.0017 | 0.0013 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Lipid Particles | 0.0088 | 0.0002 | 0.0052 | 0.0002 | 0.0091 | 0.0001 | 0.0142 | 0.0001 | 0.0058 | 0.0003 | 0.0552 | 0.0014 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Mitochondria | 0.0056 | 0.0004 | 0.0023 | 0.0001 | 0.0062 | 0.0014 | 0.0073 | 0.0001 | 0.0069 | 0.0005 | 0.0161 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
None | 0.3852 | 0.3133 | 0.3661 | 0.321 | 0.3039 | 0.3655 | 0.5109 | 0.4307 | 0.4136 | 0.4665 | 0.5 | 0.2917 | 0.3386 | 0.129 | 0.2649 | 0.2726 | 0.3455 | 0.1764 |
Nuclear Periphery | 0.0244 | 0.001 | 0.0042 | 0.0009 | 0.0195 | 0.0009 | 0.0051 | 0.0004 | 0.0012 | 0.0013 | 0.0005 | 0.0013 | 0.0032 | 0.0009 | 0.0006 | 0.0021 | 0.0006 | 0.0008 |
Nucleolus | 0.002 | 0.0003 | 0.0005 | 0.0001 | 0.0027 | 0.0001 | 0.0016 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Nucleus | 0.0055 | 0.0041 | 0.0045 | 0.0028 | 0.0092 | 0.0033 | 0.0048 | 0.0018 | 0.0034 | 0.0021 | 0.0005 | 0.0056 | 0.0024 | 0.002 | 0.0019 | 0.0049 | 0.0015 | 0.0046 |
Peroxisomes | 0.0054 | 0.0003 | 0.0024 | 0.0001 | 0.0006 | 0.0001 | 0.0118 | 0.0001 | 0.004 | 0.0003 | 0.0525 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Punctate Nuclear | 0.0219 | 0.0021 | 0.0033 | 0.0012 | 0.0046 | 0.0011 | 0.0174 | 0.0005 | 0.001 | 0.0016 | 0.0061 | 0.0021 | 0.0006 | 0.0003 | 0.0004 | 0.0006 | 0.0027 | 0.0004 |
Vacuole | 0.0027 | 0.0013 | 0.002 | 0.0007 | 0.0064 | 0.0017 | 0.0017 | 0.0005 | 0.0013 | 0.0015 | 0.0005 | 0.0007 | 0.0013 | 0.0007 | 0.0007 | 0.0015 | 0.0002 | 0.0005 |
Vacuole Periphery | 0.0017 | 0.0002 | 0.0006 | 0.0001 | 0.0045 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0.0004 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 164.7796 | 119.6522 | 103.7093 | 125.455 | 92.6033 | 141.6432 | 136.2298 | 142.0359 | 145.0596 | 114.7536 |
Translational Efficiency | 1.6216 | 1.57 | 1.9108 | 1.6239 | 1.8891 | 2.087 | 1.5857 | 1.4081 | 1.4379 | 1.4887 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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