Standard name
Human Ortholog
Description CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.99 0.99 0.99 0.97 0.93 – 0.85 0.81 0.71 0.8 1.0 0.99 0.99 0.98 0.95 0.98 0.97 0.96 0.94 0.82 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.12 – 0.29 0.33 0.44 0.27 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 1 – 0 1 1 0 0 0 0 0 0 1 1 1 0 2 2 3
Bud 0 4 1 0 0 0 3 – 3 10 3 3 3 0 1 0 0 0 0 1 1 4 7 10
Bud Neck 0 0 1 0 4 3 3 – 5 6 3 1 0 0 0 0 0 1 0 1 0 2 3 4
Bud Site 0 0 0 0 0 0 0 – 0 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 12 6 5 0 4 1 – 3 4 3 1 1 1 7 25 15 13 0 0 1 1 2 1
Cytoplasm 272 515 350 237 311 232 297 – 316 326 224 244 448 391 543 523 305 313 265 512 325 304 398 366
Endoplasmic Reticulum 2 4 1 1 2 2 2 – 3 1 3 0 1 1 0 35 7 16 0 0 2 14 6 4
Endosome 0 0 0 0 0 1 0 – 0 0 0 0 0 0 1 6 2 0 2 2 1 0 0 3
Golgi 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 2 1 1 0 1 0 0 1 1
Mitochondria 0 6 0 3 2 15 38 – 107 133 138 82 1 1 0 6 4 2 1 5 5 20 17 11
Nucleus 1 1 1 2 2 0 3 – 5 1 3 1 0 3 0 0 1 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 2 0 0 0 1 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 – 2 1 4 8 0 0 0 1 0 1 0 0 1 2 0 3
Vac/Vac Membrane 0 0 0 0 2 0 1 – 0 2 3 2 0 0 1 7 11 2 0 3 1 7 18 11
Unique Cell Count 273 525 352 239 314 240 319 373 401 316 304 450 393 546 534 320 319 275 534 346 371 464 432
Labelled Cell Count 275 544 361 249 323 257 351 444 486 389 343 454 397 554 606 346 352 275 534 346 371 464 432


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.6 5.6 5.3 5.3 4.0 4.3 – 3.9 3.9 3.5 4.0 4.9 5.1 5.1 6.9 7.3 6.7 4.7 4.7 5.3
Std Deviation (1e-4) 0.5 0.7 1.0 1.6 1.3 0.6 1.3 – 2.2 1.9 0.9 0.7 0.8 1.1 1.0 1.1 1.4 1.1 1.2 1.0 1.7
Intensity Change (Log2) – – – -0.08 -0.08 -0.5 -0.4 – -0.54 -0.53 -0.7 -0.47 -0.18 -0.13 -0.15 0.3 0.39 0.26 -0.25 -0.26 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 -0.5 2.4 2.2 1.8
Cytoplasm -0.4 -0.6 -2.6 -4.4 – -7.2 -8.2 -10.6 -8.4 0.2 0.1 0 -1.8 -3.4 -1.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 4.6 0 3.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3444 0.7233 0.3733 0.2132 0.1217 0.4736 2.4164 1.7692 1.924 1.2413 1.7824 1.5028 1.591 0.9787 0.9615 1.2733 1.2851 0.8869
Actin 0.0391 0.0022 0.0008 0.0013 0.0051 0.0056 0.0302 0.0077 0.0308 0.0027 0.0026 0.0035 0.0344 0.0021 0.0091 0.0137 0.0119 0.0217
Bud 0.0005 0.0066 0.0014 0.0003 0.0001 0.0007 0.0023 0.0042 0.0099 0.002 0.0013 0.0064 0.0102 0.0085 0.006 0.0257 0.0186 0.0013
Bud Neck 0.0013 0.0004 0.0003 0.0005 0.0007 0.0007 0.0021 0.0004 0.0026 0.0091 0.0007 0.0016 0.0077 0.0003 0.0008 0.0004 0.0021 0.0097
Bud Periphery 0.001 0.0045 0.0022 0.0004 0.0003 0.0011 0.0029 0.0052 0.0107 0.0041 0.0017 0.013 0.0125 0.0032 0.0029 0.017 0.0095 0.0021
Bud Site 0.0008 0.0188 0.0015 0.0003 0.0007 0.0003 0.017 0.0213 0.0413 0.0284 0.0005 0.001 0.0187 0.0207 0.0063 0.0026 0.0098 0.0008
Cell Periphery 0.0003 0.0004 0.0004 0.0002 0.0003 0.0002 0.0008 0.0008 0.0007 0.0009 0.0002 0.0003 0.0013 0.0005 0.0006 0.0003 0.0005 0.0001
Cytoplasm 0.2906 0.5822 0.5461 0.3271 0.4195 0.5321 0.3443 0.699 0.542 0.6596 0.6308 0.7458 0.2903 0.5443 0.4907 0.5203 0.5986 0.5348
Cytoplasmic Foci 0.0145 0.0114 0.0105 0.0221 0.02 0.0137 0.053 0.0104 0.0335 0.0117 0.0177 0.0332 0.0292 0.0079 0.0128 0.0096 0.0322 0.0129
Eisosomes 0.0007 0.0002 0.0001 0.0001 0.0011 0.0001 0.0005 0.0002 0.0003 0.0002 0 0.0001 0.0013 0.0001 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0044 0.0032 0.0049 0.005 0.0025 0.0026 0.0078 0.0036 0.0056 0.0065 0.0024 0.0048 0.0048 0.0024 0.0041 0.0017 0.0025 0.005
Endosome 0.0189 0.0032 0.009 0.0105 0.0039 0.0041 0.0941 0.0033 0.0257 0.0388 0.0297 0.0119 0.0284 0.0026 0.0152 0.0094 0.018 0.0085
Golgi 0.005 0.0004 0.0014 0.0008 0.0009 0.0005 0.0138 0.0006 0.009 0.0093 0.0248 0.0039 0.0125 0.0003 0.003 0.0005 0.0122 0.0029
Lipid Particles 0.0062 0.0006 0.0114 0.0015 0.0027 0.0005 0.0581 0.0006 0.0064 0.0183 0.0048 0.0031 0.016 0.0002 0.0059 0.0001 0.0038 0.0017
Mitochondria 0.0029 0.0005 0.0035 0.0008 0.002 0.0003 0.022 0.0007 0.0145 0.0064 0.0645 0.0087 0.0181 0.0004 0.003 0.0015 0.003 0.003
None 0.5894 0.3576 0.3912 0.6183 0.5317 0.4329 0.2211 0.2334 0.2482 0.1303 0.2093 0.1469 0.4446 0.4003 0.4166 0.3807 0.2393 0.3772
Nuclear Periphery 0.0111 0.0009 0.0014 0.0014 0.0006 0.0004 0.0187 0.0009 0.0023 0.0098 0.0007 0.0035 0.01 0.0006 0.0062 0.0008 0.0023 0.0041
Nucleolus 0.0006 0.0005 0.0008 0.0003 0.0004 0.0001 0.0038 0.0003 0.0006 0.0318 0.0001 0.0015 0.0021 0.0003 0.0006 0.0006 0.0021 0.0003
Nucleus 0.0028 0.0017 0.0023 0.0015 0.0009 0.0007 0.0401 0.0024 0.0022 0.0126 0.001 0.0035 0.0251 0.002 0.0072 0.0106 0.0172 0.0025
Peroxisomes 0.0024 0.0003 0.0029 0.0006 0.0044 0.0006 0.0288 0.0005 0.0041 0.0012 0.0022 0.0011 0.0146 0.0002 0.0022 0.0003 0.0045 0.0047
Punctate Nuclear 0.0048 0.0017 0.0012 0.0019 0.0014 0.0016 0.0135 0.001 0.002 0.0044 0.0003 0.0028 0.0114 0.0008 0.0031 0.0006 0.0065 0.0047
Vacuole 0.0019 0.0022 0.0057 0.0043 0.0007 0.0011 0.0201 0.0032 0.006 0.0088 0.0037 0.0024 0.0053 0.002 0.0029 0.0028 0.0043 0.0015
Vacuole Periphery 0.001 0.0002 0.0009 0.0005 0.0001 0.0002 0.0049 0.0003 0.0015 0.0031 0.0012 0.0009 0.0013 0.0002 0.0008 0.0006 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4917 10.1819 7.8703 13.3028 10.1527 10.9094 14.799 12.594 10.0498 12.9184
Translational Efficiency 0.7787 0.7483 0.8718 0.4454 0.4809 0.5522 0.4425 0.4921 0.5811 0.555

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gis4

Gis4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gis4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available