Standard name | |
---|---|
Human Ortholog | |
Description | Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 1 | 2 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 1 | 1 | 1 | 2 | 4 | 4 | 5 | 3 | 2 | 7 | 0 | 2 | 0 | 11 | 5 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 216 | 389 | 226 | 166 | 277 | 304 | 289 | 317 | 192 | 252 | 232 | 235 | 182 | 350 | 362 | 556 | 423 | 369 | 218 | 395 | 242 | 319 | 340 | 383 |
Endoplasmic Reticulum | 2 | 6 | 1 | 1 | 3 | 1 | 0 | 7 | 1 | 1 | 4 | 4 | 2 | 1 | 0 | 15 | 10 | 11 | 2 | 1 | 4 | 1 | 0 | 3 |
Endosome | 4 | 2 | 2 | 5 | 1 | 3 | 3 | 2 | 2 | 0 | 1 | 3 | 5 | 6 | 9 | 5 | 2 | 5 | 3 | 5 | 0 | 3 | 4 | 9 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 4 | 1 | 0 | 1 | 1 |
Mitochondria | 4 | 1 | 1 | 2 | 4 | 23 | 40 | 31 | 38 | 75 | 108 | 74 | 0 | 0 | 2 | 8 | 4 | 6 | 4 | 3 | 6 | 2 | 3 | 6 |
Nucleus | 2 | 3 | 0 | 7 | 7 | 12 | 13 | 13 | 12 | 5 | 4 | 11 | 4 | 1 | 1 | 5 | 1 | 1 | 5 | 12 | 2 | 5 | 4 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 4 | 5 | 6 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 4 | 3 |
Vac/Vac Membrane | 13 | 26 | 14 | 20 | 32 | 26 | 58 | 41 | 23 | 63 | 17 | 32 | 16 | 33 | 33 | 70 | 73 | 60 | 3 | 3 | 8 | 5 | 3 | 4 |
Unique Cell Count | 232 | 418 | 240 | 193 | 316 | 355 | 374 | 401 | 252 | 362 | 330 | 333 | 199 | 379 | 388 | 612 | 465 | 407 | 250 | 439 | 278 | 352 | 374 | 425 |
Labelled Cell Count | 242 | 429 | 245 | 205 | 325 | 371 | 410 | 425 | 287 | 412 | 372 | 375 | 209 | 393 | 409 | 672 | 522 | 465 | 250 | 439 | 278 | 352 | 374 | 425 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 7.0 | 6.9 | 6.4 | 7.4 | 6.5 | 6.4 | 6.6 | 5.9 | 5.9 | 5.3 | 5.6 | 7.8 | 7.5 | 6.8 | 8.9 | 9.1 | 8.7 | 6.5 | 6.7 | 6.8 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.1 | 1.4 | 1.6 | 1.2 | 1.2 | 1.4 | 1.4 | 1.4 | 1.1 | 1.2 | 1.1 | 0.9 | 1.3 | 1.6 | 1.7 | 1.6 | 0.9 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.1 | -0.09 | -0.11 | -0.05 | -0.24 | -0.22 | -0.38 | -0.31 | 0.17 | 0.11 | -0.01 | 0.36 | 0.4 | 0.33 | -0.1 | -0.04 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 |
Cytoplasm | -2.9 | -2.6 | -3.3 | -5.6 | -5.1 | -5.6 | -7.3 | -7.1 | -7.0 | -1.1 | -0.9 | -0.4 | -1.6 | -1.5 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.7 | 5.0 | 4.1 | 6.0 | 7.3 | 9.7 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.9 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.7 | 1.8 | 0.7 | 3.6 | 1.9 | 1.4 | 4.2 | -0.4 | 1.6 | 0.9 | 1.3 | 1.2 | 2.5 | 3.8 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0301 | 0.0004 | 0.0008 | 0.0306 | 0.0085 | 0.0028 | 0.0083 | 0.0003 | 0.0033 | 0.0007 | 0.0002 | 0.0028 | 0.0003 | 0 | 0.0008 | 0 | 0 | 0.0001 |
Bud | 0.0004 | 0.0025 | 0.0008 | 0.0007 | 0.0003 | 0.0001 | 0.0008 | 0.0004 | 0.0007 | 0.0058 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.001 | 0.0004 | 0.0016 | 0.0022 | 0.0005 | 0.0041 | 0.0025 | 0.001 | 0.0013 | 0.0011 | 0.0007 | 0.0035 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0004 |
Bud Periphery | 0.0003 | 0.0017 | 0.001 | 0.001 | 0.0004 | 0.0001 | 0.0013 | 0.0002 | 0.0006 | 0.0111 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0026 | 0.0038 | 0.0024 | 0.0197 | 0.0005 | 0.0002 | 0.0011 | 0.0085 | 0.0065 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.6767 | 0.8654 | 0.7698 | 0.7828 | 0.7475 | 0.8004 | 0.7132 | 0.8075 | 0.7644 | 0.7496 | 0.8415 | 0.7793 | 0.8751 | 0.9415 | 0.6776 | 0.8811 | 0.9728 | 0.9089 |
Cytoplasmic Foci | 0.0478 | 0.0083 | 0.0113 | 0.0138 | 0.0181 | 0.0239 | 0.0143 | 0.0066 | 0.0097 | 0.0186 | 0.0184 | 0.0135 | 0.0033 | 0.0015 | 0.016 | 0.0177 | 0.0031 | 0.0012 |
Eisosomes | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0073 | 0.0038 | 0.0078 | 0.0036 | 0.0036 | 0.0059 | 0.0062 | 0.0025 | 0.003 | 0.0024 | 0.0042 | 0.0032 | 0.0459 | 0.0007 | 0.0342 | 0.0033 | 0.0028 | 0.0019 |
Endosome | 0.0347 | 0.0092 | 0.0154 | 0.0356 | 0.0456 | 0.0129 | 0.0108 | 0.0028 | 0.0059 | 0.0091 | 0.01 | 0.0062 | 0.004 | 0.0002 | 0.0508 | 0.0069 | 0.0023 | 0.0006 |
Golgi | 0.0076 | 0.0004 | 0.0063 | 0.0107 | 0.035 | 0.0017 | 0.0014 | 0.0002 | 0.002 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0061 | 0.0001 | 0 | 0 |
Lipid Particles | 0.0156 | 0.0002 | 0.0004 | 0.0017 | 0.0598 | 0.0012 | 0.0016 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0019 | 0.0001 | 0 | 0 |
Mitochondria | 0.0036 | 0.0005 | 0.0021 | 0.0027 | 0.0138 | 0.0005 | 0.0008 | 0.0003 | 0.0033 | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0007 | 0.0003 | 0.0001 | 0.0001 |
None | 0.0997 | 0.0507 | 0.0855 | 0.0503 | 0.0301 | 0.0966 | 0.2123 | 0.1302 | 0.1387 | 0.1631 | 0.0496 | 0.1503 | 0.0472 | 0.0287 | 0.1218 | 0.0691 | 0.0048 | 0.0127 |
Nuclear Periphery | 0.0091 | 0.0026 | 0.0048 | 0.0018 | 0.0049 | 0.0039 | 0.0039 | 0.0021 | 0.0045 | 0.002 | 0.0035 | 0.0021 | 0.0057 | 0.0008 | 0.004 | 0.0019 | 0.0024 | 0.0013 |
Nucleolus | 0.001 | 0.0004 | 0.0037 | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0146 | 0.0421 | 0.0746 | 0.0339 | 0.0057 | 0.0346 | 0.0125 | 0.0318 | 0.0421 | 0.0261 | 0.0623 | 0.027 | 0.0147 | 0.0244 | 0.0808 | 0.0124 | 0.0097 | 0.0722 |
Peroxisomes | 0.03 | 0.0003 | 0.0005 | 0.0008 | 0.0058 | 0.0031 | 0.0009 | 0.0002 | 0.0014 | 0.0006 | 0.0002 | 0.0009 | 0 | 0 | 0.0003 | 0 | 0 | 0 |
Punctate Nuclear | 0.014 | 0.002 | 0.0046 | 0.0011 | 0.0158 | 0.0054 | 0.0041 | 0.0032 | 0.0089 | 0.0036 | 0.0055 | 0.0074 | 0.0007 | 0.0005 | 0.0007 | 0.0021 | 0.0005 | 0.0003 |
Vacuole | 0.003 | 0.0048 | 0.0058 | 0.0051 | 0.0021 | 0.0021 | 0.0028 | 0.0017 | 0.0019 | 0.0029 | 0.0022 | 0.0013 | 0.0017 | 0.0004 | 0.0025 | 0.0036 | 0.0007 | 0.0002 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0006 | 0.0007 | 0.0013 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0004 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.7914 | 37.8793 | 34.4792 | 43.8553 | 36.9923 | 45.738 | 50.6833 | 56.8694 | 51.6345 | 45.0857 |
Translational Efficiency | 1.5596 | 1.5657 | 1.4348 | 0.9877 | 1.076 | 1.3688 | 1.0963 | 1.0732 | 1.0966 | 1.1771 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|