Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 1 | 1 | 3 | 3 | 7 | 2 | 1 | 4 | 13 | 14 |
Bud | 5 | 1 | 14 | 13 | 2 | 3 | – | 7 | 7 | 12 | 7 | 9 | 1 | 2 | 1 | 1 | 4 | 4 | 2 | 0 | 0 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 5 | 0 | 1 | 0 | 0 | 1 | – | 3 | 4 | 5 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 5 | 4 | 6 | 2 | 2 | – | 3 | 1 | 1 | 3 | 5 | 10 | 4 | 6 | 3 | 7 | 4 | 1 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 4 | 1 | 2 | 6 | 10 | 8 | – | 20 | 5 | 2 | 3 | 3 | 8 | 6 | 21 | 7 | 7 | 6 | 1 | 0 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 9 | 4 | 4 | 2 | 0 | 1 | – | 0 | 1 | 2 | 1 | 0 | 5 | 15 | 3 | 2 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 1 |
Endosome | 26 | 0 | 1 | 1 | 26 | 3 | – | 14 | 1 | 4 | 1 | 0 | 16 | 19 | 28 | 4 | 15 | 8 | 48 | 2 | 14 | 15 | 28 | 31 |
Golgi | 16 | 8 | 1 | 0 | 6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 47 | 42 | 54 | 9 | 17 | 10 | 26 | 18 | 5 | 12 | 41 | 33 |
Mitochondria | 416 | 108 | 213 | 276 | 217 | 310 | – | 468 | 329 | 396 | 322 | 366 | 245 | 276 | 269 | 5 | 6 | 8 | 386 | 94 | 167 | 120 | 186 | 222 |
Nucleus | 6 | 1 | 1 | 0 | 2 | 0 | – | 4 | 0 | 4 | 3 | 3 | 0 | 0 | 1 | 2 | 1 | 2 | 3 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 17 | 4 | 9 | 5 | 11 | 36 | – | 56 | 35 | 33 | 25 | 43 | 17 | 12 | 4 | 0 | 0 | 0 | 7 | 1 | 0 | 0 | 4 | 3 |
Nucleolus | 23 | 2 | 8 | 20 | 20 | 38 | – | 77 | 33 | 34 | 38 | 27 | 8 | 12 | 1 | 2 | 1 | 1 | 4 | 0 | 0 | 0 | 3 | 4 |
Peroxisomes | 3 | 1 | 2 | 5 | 14 | 1 | – | 9 | 2 | 10 | 1 | 0 | 11 | 6 | 9 | 3 | 7 | 3 | 10 | 1 | 22 | 11 | 26 | 26 |
SpindlePole | 4 | 0 | 3 | 2 | 4 | 4 | – | 12 | 6 | 7 | 8 | 5 | 1 | 2 | 5 | 3 | 2 | 5 | 5 | 0 | 0 | 0 | 6 | 7 |
Vac/Vac Membrane | 129 | 17 | 10 | 4 | 24 | 16 | – | 16 | 17 | 8 | 6 | 4 | 46 | 50 | 75 | 16 | 31 | 26 | 42 | 5 | 1 | 11 | 17 | 22 |
Unique Cell Count | 513 | 119 | 218 | 282 | 268 | 340 | 564 | 341 | 426 | 337 | 388 | 295 | 333 | 353 | 48 | 79 | 66 | 562 | 130 | 218 | 183 | 336 | 383 | |
Labelled Cell Count | 680 | 154 | 273 | 340 | 338 | 423 | 689 | 441 | 518 | 423 | 470 | 420 | 453 | 479 | 58 | 103 | 84 | 562 | 130 | 218 | 183 | 336 | 383 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.3 | 5.4 | 6.2 | 7.2 | 5.4 | – | 5.6 | 5.0 | 5.3 | 5.2 | 4.8 | 7.9 | 7.5 | 7.5 | 8.1 | 8.7 | 8.3 | 6.9 | 7.9 | 8.1 |
Std Deviation (1e-4) | 1.1 | 1.7 | 1.2 | 1.5 | 2.6 | 1.6 | – | 1.6 | 1.5 | 1.3 | 1.6 | 1.3 | 1.2 | 1.3 | 1.6 | 1.9 | 2.1 | 2.0 | 1.4 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.22 | 0.42 | 0.02 | – | 0.06 | -0.11 | -0.03 | -0.04 | -0.15 | 0.55 | 0.49 | 0.48 | 0.6 | 0.69 | 0.64 | 0.37 | 0.56 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.9 | -3.5 | -3.7 | – | -4.0 | -2.6 | -2.2 | -2.6 | 0 | -4.0 | -4.0 | -4.5 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.0 | 0 | – | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.2 | 4.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.9 | 5.2 | 5.8 | 0 | 0 | 0 |
Mitochondria | 0.1 | -5.7 | -3.1 | – | -5.5 | -0.8 | -2.5 | -1.3 | 0 | -5.3 | -5.4 | -6.9 | -14.2 | -15.6 | -14.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.6 | 0 | 2.7 | – | 2.6 | 2.6 | 1.8 | 1.6 | 0 | 0.8 | -0.3 | 0 | 0 | 0 | 0 |
Nucleolus | 1.7 | 1.8 | 3.1 | – | 4.0 | 2.7 | 2.1 | 3.2 | 0 | -0.6 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | 1.9 | 0.1 | – | -1.2 | 0.2 | -2.0 | -1.9 | -2.8 | 4.0 | 3.8 | 5.4 | 0 | 7.6 | 7.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4179 | 4.3707 | 4.1941 | 3.9984 | 3.3876 | 4.2807 | 4.1336 | 4.0496 | 3.7677 | 3.5674 | 3.4209 | 3.7744 | 6.0082 | 5.7832 | 5.7331 | 5.7406 | 4.9797 | 5.7831 |
Actin | 0.014 | 0.006 | 0.0023 | 0.0031 | 0.0133 | 0.0046 | 0.0427 | 0.0293 | 0.0291 | 0.0433 | 0.003 | 0.0104 | 0.0402 | 0.014 | 0.0096 | 0.001 | 0.0014 | 0.0012 |
Bud | 0.0005 | 0.0096 | 0.0033 | 0.0008 | 0.0001 | 0.0001 | 0.001 | 0.0006 | 0.0006 | 0.0004 | 0.0007 | 0.001 | 0.0006 | 0.0004 | 0.0004 | 0 | 0.0069 | 0.0001 |
Bud Neck | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0031 | 0.0004 | 0.0005 | 0.0007 | 0.0001 | 0.004 | 0.0057 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0004 |
Bud Periphery | 0.0008 | 0.0027 | 0.0019 | 0.0021 | 0.0003 | 0.0004 | 0.002 | 0.0008 | 0.0009 | 0.0008 | 0.0014 | 0.0091 | 0.0015 | 0.0009 | 0.0007 | 0.0001 | 0.0066 | 0.0001 |
Bud Site | 0.001 | 0.0143 | 0.0017 | 0.0007 | 0.0002 | 0.0002 | 0.0135 | 0.0045 | 0.0019 | 0.0005 | 0.0003 | 0.0007 | 0.0031 | 0.0026 | 0.0011 | 0 | 0.0051 | 0.0001 |
Cell Periphery | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0012 | 0.0004 | 0.0013 | 0.0007 | 0.0003 | 0.0004 | 0.008 | 0.0004 | 0.0015 | 0.0001 | 0.0005 | 0.0034 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0024 | 0.0008 |
Cytoplasmic Foci | 0.0081 | 0.0093 | 0.0062 | 0.0225 | 0.0049 | 0.0047 | 0.0123 | 0.0103 | 0.0083 | 0.0011 | 0.0031 | 0.006 | 0.0074 | 0.0037 | 0.0031 | 0.0003 | 0.0281 | 0.0057 |
Eisosomes | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0012 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0014 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.001 | 0.0122 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0019 | 0.0015 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0002 |
Endosome | 0.0224 | 0.0111 | 0.0112 | 0.0169 | 0.0186 | 0.0194 | 0.0493 | 0.0325 | 0.0187 | 0.0066 | 0.0232 | 0.0301 | 0.0302 | 0.0108 | 0.0112 | 0.0005 | 0.0409 | 0.014 |
Golgi | 0.0391 | 0.0286 | 0.0258 | 0.0251 | 0.0473 | 0.0342 | 0.033 | 0.0605 | 0.0271 | 0.0103 | 0.0186 | 0.0516 | 0.0344 | 0.0238 | 0.0439 | 0.0017 | 0.0318 | 0.0212 |
Lipid Particles | 0.0216 | 0.0026 | 0.0058 | 0.0128 | 0.0088 | 0.0178 | 0.0377 | 0.0062 | 0.0042 | 0.0006 | 0.0034 | 0.0027 | 0.0234 | 0.007 | 0.0033 | 0.0002 | 0.0028 | 0.0209 |
Mitochondria | 0.8433 | 0.8856 | 0.9012 | 0.8937 | 0.8781 | 0.8975 | 0.7323 | 0.8186 | 0.8854 | 0.9307 | 0.9293 | 0.8444 | 0.8169 | 0.9163 | 0.9112 | 0.9917 | 0.8454 | 0.9217 |
None | 0.0071 | 0.0001 | 0.0065 | 0.001 | 0.0004 | 0.0002 | 0.0019 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0092 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0008 |
Nuclear Periphery | 0.0028 | 0.0001 | 0.0003 | 0.0002 | 0.0135 | 0.0012 | 0.0112 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0024 | 0.0017 | 0.0006 | 0 | 0 | 0.0003 | 0.0002 |
Nucleolus | 0.0009 | 0.0006 | 0.0008 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0024 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0029 | 0.0003 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 |
Peroxisomes | 0.0203 | 0.0233 | 0.0216 | 0.0146 | 0.0081 | 0.013 | 0.0214 | 0.0266 | 0.0098 | 0.0011 | 0.0091 | 0.0027 | 0.0217 | 0.0118 | 0.0122 | 0.0041 | 0.0218 | 0.0079 |
Punctate Nuclear | 0.0035 | 0 | 0.0019 | 0.0008 | 0.0006 | 0.0001 | 0.0013 | 0 | 0.0013 | 0 | 0 | 0.0017 | 0.0003 | 0.0002 | 0 | 0 | 0.0007 | 0.0004 |
Vacuole | 0.0045 | 0.0013 | 0.0024 | 0.0017 | 0.0006 | 0.0011 | 0.005 | 0.0039 | 0.0037 | 0.0008 | 0.0023 | 0.0039 | 0.0029 | 0.0028 | 0.0006 | 0 | 0.0026 | 0.0011 |
Vacuole Periphery | 0.0068 | 0.0027 | 0.0048 | 0.0016 | 0.0029 | 0.0037 | 0.0088 | 0.004 | 0.0033 | 0.002 | 0.0041 | 0.0086 | 0.0051 | 0.0035 | 0.0016 | 0.0002 | 0.0018 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 75.1144 | 64.8322 | 89.344 | 54.4523 | 57.0902 | 98.3713 | 72.5752 | 74.2651 | 103.1005 | 75.7379 |
Translational Efficiency | 1.5119 | 1.7062 | 1.025 | 1.949 | 2.1275 | 1.2539 | 1.7086 | 2.0636 | 1.3819 | 1.3365 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|