Standard name | |
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Human Ortholog | |
Description | Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and in concert with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; interacts with DNA upstream of RNAPII and the non-template strand of the transcription bubble; Spt5p is the only transcription elongation factor conserved in all domains of life |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2 | 0 | 4 | 4 | 4 | 5 | – | 0 | 2 | 1 | 3 | 7 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 3 | 2 | 7 | 2 | 11 | 8 | 5 | – | 8 | 9 | 4 | 34 | 29 | 7 | 14 | 17 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 3 | 2 | 7 | 4 | 8 | 15 | 34 | 24 | – | 20 | 28 | 1 | 2 | 14 | 5 | 4 | 15 | 0 | 0 | 1 | 0 | 0 | 2 |
Nucleus | 154 | 211 | 144 | 226 | 221 | 159 | 256 | 292 | 160 | – | 100 | 180 | 66 | 288 | 273 | 137 | 115 | 172 | 133 | 199 | 116 | 129 | 202 | 178 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 3 | – | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 106 | 138 | 112 | 115 | 69 | 106 | 191 | 266 | 163 | – | 82 | 199 | 53 | 152 | 170 | 55 | 58 | 75 | 51 | 45 | 56 | 35 | 104 | 150 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 1 | 1 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 5 | 16 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 1 | 2 | 1 | 6 | 1 | – | 1 | 2 | 1 | 2 | 3 | 4 | 4 | 7 | 2 | 3 | 4 | 4 | 16 | 24 |
Unique Cell Count | 180 | 249 | 175 | 271 | 258 | 218 | 362 | 482 | 261 | 162 | 321 | 83 | 380 | 385 | 165 | 161 | 230 | 192 | 254 | 185 | 172 | 335 | 374 | |
Labelled Cell Count | 262 | 355 | 262 | 356 | 305 | 283 | 484 | 616 | 363 | 215 | 424 | 128 | 488 | 526 | 212 | 200 | 289 | 192 | 254 | 185 | 172 | 335 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.6 | 24.5 | 16.3 | 16.4 | 15.8 | 13.7 | 12.7 | 12.5 | 11.5 | – | 12.4 | 11.3 | 20.5 | 21.6 | 17.4 | 20.5 | 23.7 | 22.7 | 23.8 | 23.5 | 23.9 |
Std Deviation (1e-4) | 7.4 | 7.0 | 4.0 | 4.8 | 7.0 | 3.4 | 3.7 | 4.3 | 3.7 | – | 6.2 | 4.0 | 7.0 | 9.5 | 7.5 | 8.4 | 10.3 | 9.8 | 7.8 | 8.7 | 9.0 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.05 | -0.25 | -0.37 | -0.39 | -0.5 | – | -0.4 | -0.53 | 0.33 | 0.41 | 0.1 | 0.33 | 0.54 | 0.47 | 0.55 | 0.52 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | – | 1.7 | 0 | 0 | 3.2 | 2.7 | 0 | 2.9 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1.9 | 2.9 | 3.5 | – | 4.2 | 3.4 | 0 | 0 | 1.6 | 0 | 0 | 2.7 |
Nucleus | 0.3 | 0.9 | -2.2 | -2.9 | -5.2 | -4.7 | – | -4.2 | -5.9 | -0.5 | -1.7 | -2.9 | 0.2 | -2.4 | -1.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -4.4 | -7.7 | -3.0 | -2.5 | -2.0 | -0.3 | – | -2.5 | -0.4 | 0 | -5.3 | -4.4 | -5.7 | -5.1 | -6.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.666 | 18.6907 | 15.9258 | 14.1987 | 12.7109 | 16.1861 | 19.9045 | 23.3857 | 20.6885 | 19.1076 | 19.7875 | 20.6811 | 17.459 | 17.7696 | 16.7738 | 16.3126 | 15.8207 | 17.0973 |
Actin | 0.0917 | 0.0034 | 0.0065 | 0.0001 | 0.0767 | 0 | 0.0004 | 0.0046 | 0.0067 | 0.0809 | 0.0008 | 0.0003 | 0.0009 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.002 | 0.0001 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.004 | 0.0004 | 0.0007 | 0.0001 | 0.0031 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0021 | 0.0003 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0134 | 0.0154 | 0.0004 | 0.0005 | 0.01 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0012 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0008 | 0.0011 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0052 | 0.0171 | 0.0075 | 0.0002 | 0.0064 | 0.0003 | 0.0142 | 0 | 0.0048 | 0 | 0.001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0329 | 0 | 0.0047 | 0.0121 | 0.0038 | 0.0034 | 0.002 | 0.0017 | 0.004 | 0.001 | 0.0016 | 0.0002 | 0.009 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0068 | 0.0001 | 0.0028 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0064 | 0.0001 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0378 | 0.0003 | 0.0054 | 0.0002 | 0.0027 | 0.0001 | 0.004 | 0.0017 | 0.0085 | 0.0008 | 0.0178 | 0.0022 | 0.0008 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0218 | 0.0003 | 0.0018 | 0.0001 | 0.0091 | 0 | 0.0001 | 0.0045 | 0.0026 | 0.0037 | 0.0011 | 0.0007 | 0.0014 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0382 | 0.0001 | 0.0037 | 0.0111 | 0.0013 | 0.0014 | 0.0004 | 0.0004 | 0.002 | 0.0009 | 0.0036 | 0.0007 | 0.0118 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0237 | 0.011 | 0.014 | 0.0065 | 0.0063 | 0.0021 | 0.0046 | 0.0026 | 0.0024 | 0.0037 | 0.0467 | 0.0013 | 0.001 | 0.0002 | 0.0003 | 0.0008 | 0.0009 | 0.0004 |
None | 0.0041 | 0.0001 | 0.0006 | 0.0007 | 0.0822 | 0.0003 | 0.0044 | 0 | 0.0007 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0079 | 0.0004 | 0.0053 | 0.0004 | 0.0011 | 0.0001 | 0.0097 | 0.0001 | 0.0069 | 0 | 0.0091 | 0.0026 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0002 |
Nucleolus | 0.166 | 0.1463 | 0.0867 | 0.1922 | 0.4634 | 0.3652 | 0.1755 | 0.0828 | 0.05 | 0.0813 | 0.469 | 0.2642 | 0.0967 | 0.0844 | 0.1151 | 0.0498 | 0.5195 | 0.268 |
Nucleus | 0.5094 | 0.8029 | 0.8444 | 0.7687 | 0.3163 | 0.623 | 0.7738 | 0.8946 | 0.9014 | 0.8232 | 0.4329 | 0.7184 | 0.8403 | 0.9152 | 0.8845 | 0.9491 | 0.4778 | 0.7309 |
Peroxisomes | 0.0097 | 0.0001 | 0.0028 | 0.0008 | 0.0012 | 0.0001 | 0.0002 | 0.0059 | 0.0006 | 0.0019 | 0.0008 | 0.0001 | 0.037 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0157 | 0 | 0.0109 | 0.0027 | 0.0125 | 0.0011 | 0.0069 | 0.0001 | 0.0013 | 0.0001 | 0.0034 | 0.0073 | 0.0004 | 0 | 0 | 0 | 0.0005 | 0.0001 |
Vacuole | 0.0031 | 0.0002 | 0.0005 | 0.001 | 0.0004 | 0.0007 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0035 | 0.0005 | 0 | 0 | 0 | 0 | 0.0002 | 0 |
Vacuole Periphery | 0.0029 | 0.0002 | 0.0008 | 0.0016 | 0.0003 | 0.0016 | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0.0042 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 64.469 | 61.185 | 69.2494 | 81.2894 | 81.57 | 71.1703 | 69.2846 | 72.7533 | 82.7781 | 79.9809 |
Translational Efficiency | 3.0145 | 3.0636 | 2.3099 | 2.337 | 2.257 | 2.2594 | 1.8322 | 2.0049 | 1.6981 | 1.7088 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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