Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24,
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.11 0.12 0.07 0.09 0.14 0.13 0.14 0.18 0.15 0.17 0 0.06 0 0.06 0.11 0.1 0 0 0 0.06 0.08 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.05 0 0.08 0.11 0.08 0.11 0.07 0.07 0.13 0.59 0.11 0.33 0 0 0 0 0 0
Cytoplasm 0.1 0.1 0.08 0.15 0.14 0.18 0.13 0.13 0.16 0.08 0.13 0.13 0.08 0.07 0.08 0.24 0.37 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0.89 0.91 0.88 0.79 0.84 0.79 0.77 0.72 0.59 0.58 0.6 0.54 0.91 0.88 0.85 0 0 0.05 0.89 0.95 0.91 0.81 0.78 0.79
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0.05 0.05 0.08 0.09 0.07 0.06 0.05 0.06 0 0.12 0.34 0.25 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0.09 0.18 0.16 0.22 0.22 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.05 0.11 0.05 0.06 0 0 0 0 0.11 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 13 18 16 17 26 49 41 38 64 48 68 8 19 7 1 4 4 3 1 3 14 22 18
Bud 2 0 1 1 2 2 3 10 6 10 15 11 0 1 0 0 0 0 3 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 9 3 6 10 15 17 9 21 38 25 42 24 21 41 10 4 13 0 0 1 6 11 11
Cytoplasm 25 28 13 21 33 52 45 43 43 28 41 52 26 21 25 4 13 13 7 6 3 5 7 8
Endoplasmic Reticulum 225 263 138 108 195 223 260 229 161 203 193 214 315 261 266 0 0 2 238 287 151 200 236 274
Endosome 0 0 0 0 1 1 1 2 1 7 0 0 0 1 1 0 0 0 0 0 0 1 1 0
Golgi 9 3 4 10 9 4 16 17 23 31 23 22 17 19 8 2 12 10 1 1 1 6 10 12
Mitochondria 0 1 3 5 4 13 24 29 49 57 71 88 0 0 0 1 2 2 1 0 0 2 2 6
Nucleus 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 2 0 0 1 3 3 6 1 6 10 2 5 0 0 0 0 0 0 2 1 0 0 0 0
Nucleolus 2 0 0 0 0 0 1 2 3 5 5 3 0 1 1 0 0 0 2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 6 2 2 3 4 2 12 17 15 37 15 25 5 5 3 0 4 0 1 1 1 4 2 3
Unique Cell Count 254 289 157 136 232 284 339 320 275 350 323 393 345 298 312 17 35 40 269 303 166 247 305 347
Labelled Cell Count 282 319 182 171 278 341 436 402 367 490 439 530 395 349 353 18 39 44 269 303 166 247 305 347


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.4 40.3 23.7 23.2 23.0 19.6 19.4 19.4 18.8 17.5 18.9 18.9 33.4 32.9 32.0 42.7 43.8 44.6 33.8 34.3 34.1
Std Deviation (1e-4) 8.8 6.6 5.3 5.7 4.8 4.2 4.1 4.6 4.7 3.7 4.7 4.6 7.5 7.3 6.3 10.3 13.0 13.5 7.5 7.6 8.7
Intensity Change (Log2) – – – -0.03 -0.04 -0.28 -0.29 -0.29 -0.33 -0.44 -0.33 -0.33 0.49 0.47 0.43 0.85 0.88 0.91 0.51 0.53 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.1 -1.4 -0.8 0.9 0.4 0.7 1.9 1.0 1.7 -4.3 -1.9 -4.2 0 0 0
Bud 0 0 0 0 0 0 0 2.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.3 1.7 1.6 0 2.5 3.4 2.6 3.4 2.3 2.3 3.9 0 0 0
Cytoplasm 1.9 1.8 2.8 1.6 1.6 2.2 -0.1 1.4 1.6 -0.3 -0.5 -0.1 0 0 4.0
Endoplasmic Reticulum -2.0 -1.1 -2.4 -2.9 -4.0 -6.4 -6.6 -6.3 -7.4 1.2 -0.1 -0.8 -8.5 -10.5 -10.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.9 0.7 0 1.1 1.4 2.4 2.6 2.0 1.5 1.2 1.8 0 0 0 0
Mitochondria 0 0 1.4 2.4 2.9 4.9 4.6 5.7 5.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.1 2.1 3.6 1.9 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 33.6626 40.8907 38.7823 37.4333 33.5462 38.7681 34.4127 40.8635 36.5257 33.0278 38.661 35.4292 41.1645 43.9843 45.2433 42.1051 42.39 41.6794
Actin 0.0191 0.0005 0.0145 0.0006 0.0023 0.0009 0.0184 0.0007 0.0255 0.0006 0 0.0108 0.003 0 0 0 0 0.0005
Bud 0.0002 0 0.0001 0 0.0001 0.0002 0.0002 0.0002 0.0001 0 0 0 0 0 0 0 0 0
Bud Neck 0.0023 0.0001 0.0003 0.0001 0.0035 0.0002 0.0011 0.0002 0.0003 0 0.0001 0.0009 0.0001 0.0001 0 0 0 0.0003
Bud Periphery 0.0004 0 0.0002 0.0001 0.0002 0.0004 0.0004 0.0004 0.0002 0.0001 0 0.0002 0.0001 0 0 0 0 0
Bud Site 0.002 0 0.0004 0.0001 0.0002 0 0.0088 0.0043 0.0016 0 0 0.0001 0.0005 0 0 0 0 0
Cell Periphery 0.0094 0.0065 0.0071 0.0033 0.0038 0.0016 0.0102 0.0131 0.0033 0.0029 0.0014 0.0018 0.0032 0.0047 0.004 0.0087 0.0023 0.0024
Cytoplasm 0.0073 0.0324 0.0105 0.0006 0.0271 0.0027 0.0086 0.0069 0.0031 0.0008 0.0008 0.0017 0.0057 0.007 0.001 0.0003 0.0005 0.0018
Cytoplasmic Foci 0.0126 0.0009 0.0025 0.0011 0.0217 0.0005 0.0146 0.0001 0.0138 0.0009 0.0003 0.0003 0.0006 0.0001 0 0 0.0001 0.0004
Eisosomes 0.0023 0.0001 0.0006 0.004 0.0001 0.0003 0.001 0.0001 0.0007 0.0003 0 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.8326 0.9133 0.8995 0.8954 0.8311 0.9543 0.8476 0.9452 0.8982 0.8567 0.9535 0.92 0.9392 0.9717 0.9832 0.9769 0.9513 0.9606
Endosome 0.0108 0.0012 0.0027 0.0088 0.0062 0.0049 0.0199 0.0007 0.0039 0.0201 0.0013 0.005 0.0021 0.0003 0.0004 0.0003 0.0006 0.0019
Golgi 0.0104 0.0078 0.0077 0.016 0.014 0.0135 0.0086 0.0182 0.0086 0.0438 0.0049 0.0152 0.0061 0.0005 0.0014 0.0007 0.0203 0.0128
Lipid Particles 0.0185 0.0262 0.0316 0.023 0.0119 0.0025 0.0135 0.0008 0.0097 0.0233 0.0277 0.0021 0.0069 0.0041 0.0006 0.0014 0.0184 0.0062
Mitochondria 0.0092 0 0.0062 0.0342 0.0014 0.0019 0.0015 0.0001 0.0019 0.0017 0 0.0013 0.0009 0 0 0 0 0
None 0.0046 0.0001 0.0008 0.0005 0.0007 0.0008 0.0017 0 0.0023 0.0007 0 0.0001 0.0002 0 0 0 0 0
Nuclear Periphery 0.0336 0.0088 0.0075 0.0045 0.0179 0.0127 0.0246 0.0071 0.0122 0.0327 0.0083 0.0338 0.0265 0.0096 0.0078 0.01 0.0056 0.0113
Nucleolus 0.0008 0 0.0002 0.0001 0.005 0 0.0004 0 0.0016 0 0 0 0.0001 0 0 0 0 0
Nucleus 0.002 0.0002 0.0032 0.0001 0.0058 0.0003 0.0015 0.0003 0.0013 0.0002 0.0001 0.0006 0.0003 0.0002 0.0001 0 0 0.0001
Peroxisomes 0.0067 0 0.0007 0.0033 0.0292 0 0.0011 0 0.0043 0 0 0.0001 0.0007 0 0 0 0 0
Punctate Nuclear 0.0055 0.0001 0.0006 0 0.0049 0.0001 0.0062 0 0.004 0.0001 0 0 0.0001 0 0 0 0 0
Vacuole 0.0065 0.0016 0.0022 0.0024 0.0091 0.0012 0.007 0.001 0.0025 0.0028 0.001 0.0025 0.002 0.0015 0.001 0.0009 0.0004 0.0008
Vacuole Periphery 0.0032 0.0002 0.0008 0.0019 0.0037 0.0009 0.0031 0.0003 0.001 0.0123 0.0005 0.0034 0.0014 0.0001 0.0003 0.0002 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 310.5494 251.2468 171.475 200.8341 259.8046 251.4231 243.4505 216.4377 230.1009 257.0634
Translational Efficiency 1.0644 1.1535 1.1484 1.1383 1.0528 1.2587 1.1409 0.8705 0.9489 1.0067

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24,
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Erv25

Erv25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erv25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available