Standard name | |
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Human Ortholog | |
Description | Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 7 | – | 2 | 1 | 3 | 2 | – | 1 | 0 | 0 | 0 | – | 0 | 0 | 9 | 11 | 8 | 6 | 2 | 0 | 1 | 0 | 0 |
Bud | 1 | 1 | – | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | – | 0 | 1 | 3 | 3 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 9 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 21 | – | 8 | 11 | 13 | 15 | – | 3 | 12 | 5 | 8 | – | 3 | 10 | 28 | 20 | 22 | 1 | 3 | 0 | 0 | 4 | 12 |
Endoplasmic Reticulum | 105 | 111 | – | 27 | 41 | 57 | 51 | – | 16 | 23 | 4 | 10 | – | 13 | 25 | 8 | 6 | 6 | 150 | 134 | 16 | 4 | 21 | 28 |
Endosome | 3 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 1 | 1 | 1 | – | 1 | 0 | 2 | 6 | 3 | 3 | 3 | 0 | 0 | 2 | 1 |
Golgi | 3 | 6 | – | 3 | 1 | 2 | 1 | – | 1 | 1 | 0 | 0 | – | 1 | 0 | 185 | 104 | 56 | 2 | 5 | 0 | 0 | 2 | 0 |
Mitochondria | 22 | 3 | – | 2 | 0 | 5 | 8 | – | 14 | 21 | 35 | 31 | – | 0 | 0 | 17 | 11 | 7 | 5 | 5 | 0 | 1 | 5 | 1 |
Nucleus | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 3 | 4 | 1 | 3 | – | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 8 | 0 | – | 0 | 1 | 0 | 0 | – | 2 | 3 | 2 | 2 | – | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | – | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 13 | 22 | – | 8 | 6 | 9 | 42 | – | 15 | 43 | 16 | 30 | – | 3 | 9 | 100 | 86 | 52 | 7 | 6 | 1 | 0 | 6 | 14 |
Unique Cell Count | 141 | 150 | 45 | 51 | 80 | 106 | 48 | 89 | 59 | 79 | 18 | 43 | 282 | 200 | 129 | 180 | 165 | 21 | 8 | 49 | 66 | |||
Labelled Cell Count | 180 | 174 | 51 | 63 | 93 | 124 | 55 | 110 | 64 | 85 | 21 | 45 | 365 | 252 | 163 | 180 | 165 | 21 | 8 | 49 | 66 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 11.3 | – | 9.0 | 10.3 | 8.6 | 8.7 | – | 7.6 | 7.7 | 7.2 | 7.4 | – | 11.4 | 10.9 | 18.2 | 16.9 | 16.3 | – | – | 9.9 |
Std Deviation (1e-4) | 1.6 | 2.1 | – | 1.3 | 1.3 | 1.3 | 1.4 | – | 1.2 | 1.3 | 1.2 | 1.2 | – | 1.2 | 1.5 | 4.5 | 3.4 | 4.7 | – | – | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0432 | 6.1161 | 5.6453 | 5.0814 | 4.2418 | 5.5561 | 6.3782 | 6.7447 | 6.6001 | 5.9523 | 5.0457 | 6.3626 | 5.1991 | 5.61 | 5.1644 | 5.1263 | 3.949 | 5.5572 |
Actin | 0.0055 | 0.0012 | 0.0043 | 0.0044 | 0.0058 | 0.0024 | 0.0137 | 0.0013 | 0.0074 | 0.0051 | 0.0033 | 0.0122 | 0.0198 | 0.0016 | 0.0215 | 0.0012 | 0.0582 | 0.001 |
Bud | 0.0005 | 0.0005 | 0.0018 | 0.0007 | 0.001 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0008 | 0.0001 |
Bud Neck | 0.0006 | 0.0005 | 0.0004 | 0.0014 | 0.0014 | 0.0011 | 0.0005 | 0.0002 | 0.0005 | 0.0008 | 0.0004 | 0.0149 | 0.0008 | 0.0006 | 0.0007 | 0.0009 | 0.0019 | 0.0005 |
Bud Periphery | 0.0007 | 0.0007 | 0.0014 | 0.0007 | 0.0018 | 0.0006 | 0.0006 | 0.0001 | 0.0004 | 0.0008 | 0.0004 | 0.001 | 0.0005 | 0.0002 | 0.0005 | 0.0008 | 0.0011 | 0.0001 |
Bud Site | 0.0013 | 0.008 | 0.0019 | 0.0038 | 0.0025 | 0.0004 | 0.0014 | 0.0007 | 0.0027 | 0.0014 | 0.0003 | 0.0004 | 0.0009 | 0.0017 | 0.002 | 0.0002 | 0.0024 | 0.0001 |
Cell Periphery | 0.001 | 0.0016 | 0.0011 | 0.0006 | 0.0008 | 0.0009 | 0.0008 | 0.0008 | 0.0009 | 0.001 | 0.0005 | 0.0015 | 0.0008 | 0.0012 | 0.0007 | 0.0014 | 0.0007 | 0.0005 |
Cytoplasm | 0.0306 | 0.0404 | 0.0147 | 0.0083 | 0.0165 | 0.0196 | 0.0257 | 0.0556 | 0.0481 | 0.0233 | 0.0256 | 0.047 | 0.0236 | 0.0467 | 0.0421 | 0.0095 | 0.0338 | 0.035 |
Cytoplasmic Foci | 0.033 | 0.0112 | 0.0174 | 0.014 | 0.0419 | 0.0195 | 0.0382 | 0.0244 | 0.0174 | 0.0277 | 0.0308 | 0.0182 | 0.0186 | 0.0191 | 0.0244 | 0.0033 | 0.0387 | 0.0101 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.4249 | 0.6621 | 0.6369 | 0.6172 | 0.3271 | 0.6133 | 0.5392 | 0.6463 | 0.6607 | 0.5739 | 0.5732 | 0.6078 | 0.562 | 0.6332 | 0.674 | 0.7724 | 0.4984 | 0.6237 |
Endosome | 0.1232 | 0.0805 | 0.1163 | 0.1104 | 0.1648 | 0.0864 | 0.0878 | 0.1076 | 0.0945 | 0.1177 | 0.135 | 0.0821 | 0.0959 | 0.1169 | 0.0831 | 0.0782 | 0.1003 | 0.1014 |
Golgi | 0.023 | 0.0135 | 0.0328 | 0.0509 | 0.083 | 0.0252 | 0.0275 | 0.015 | 0.0268 | 0.0511 | 0.07 | 0.0265 | 0.0145 | 0.0186 | 0.0233 | 0.0141 | 0.0453 | 0.0204 |
Lipid Particles | 0.0903 | 0.0732 | 0.0592 | 0.0501 | 0.0935 | 0.0815 | 0.0862 | 0.0766 | 0.0653 | 0.1141 | 0.0653 | 0.0322 | 0.0672 | 0.0794 | 0.0374 | 0.0159 | 0.0656 | 0.061 |
Mitochondria | 0.0296 | 0.0068 | 0.0267 | 0.0299 | 0.1481 | 0.0144 | 0.0093 | 0.0032 | 0.0054 | 0.0066 | 0.0134 | 0.0227 | 0.0132 | 0.003 | 0.0123 | 0.0096 | 0.024 | 0.005 |
None | 0.0211 | 0.0022 | 0.0013 | 0.0004 | 0.002 | 0.0048 | 0.0083 | 0.0011 | 0.0008 | 0.0016 | 0.0008 | 0.0007 | 0.0076 | 0.0007 | 0.0022 | 0.0005 | 0.0024 | 0.0006 |
Nuclear Periphery | 0.1247 | 0.0502 | 0.0468 | 0.0344 | 0.023 | 0.0731 | 0.0879 | 0.044 | 0.046 | 0.0195 | 0.0416 | 0.1005 | 0.1165 | 0.0455 | 0.0473 | 0.0653 | 0.0481 | 0.1089 |
Nucleolus | 0.0052 | 0.0011 | 0.0016 | 0.0006 | 0.004 | 0.0036 | 0.0007 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0 | 0.0014 | 0.0001 |
Nucleus | 0.0137 | 0.016 | 0.0035 | 0.0467 | 0.0149 | 0.0132 | 0.0137 | 0.0021 | 0.002 | 0.0017 | 0.0012 | 0.0035 | 0.0044 | 0.003 | 0.002 | 0.0018 | 0.006 | 0.0055 |
Peroxisomes | 0.0176 | 0.0006 | 0.0013 | 0.0027 | 0.011 | 0.0013 | 0.0088 | 0.0007 | 0.001 | 0.0153 | 0.0214 | 0.0028 | 0.017 | 0.0006 | 0.006 | 0.0001 | 0.0064 | 0.0002 |
Punctate Nuclear | 0.015 | 0.0022 | 0.0021 | 0.0052 | 0.0224 | 0.0107 | 0.0289 | 0.0012 | 0.0024 | 0.0117 | 0.0009 | 0.0016 | 0.0048 | 0.0021 | 0.0036 | 0.0002 | 0.026 | 0.0011 |
Vacuole | 0.0232 | 0.0216 | 0.0206 | 0.011 | 0.0233 | 0.0168 | 0.0149 | 0.0153 | 0.0118 | 0.0187 | 0.0071 | 0.0153 | 0.0198 | 0.0208 | 0.0096 | 0.015 | 0.0293 | 0.0159 |
Vacuole Periphery | 0.015 | 0.0058 | 0.0076 | 0.0064 | 0.0106 | 0.0104 | 0.0056 | 0.0035 | 0.0056 | 0.0068 | 0.0082 | 0.0085 | 0.0109 | 0.0044 | 0.0057 | 0.0092 | 0.009 | 0.0089 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.9781 | 16.2991 | 21.6869 | 20.5605 | 22.0094 | 36.4456 | 25.5724 | 23.0129 | 24.8259 | 25.2031 |
Translational Efficiency | 0.7705 | 1.2101 | 0.9365 | 1.0976 | 0.8677 | 0.6493 | 0.7188 | 0.9542 | 0.9432 | 0.8495 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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