Standard name
Human Ortholog
Description Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0.06 0.06 0 0 0 0.15 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.14 – 0.18 0.22 0.16 0.14 – 0.06 0.13 0.08 0.1 – 0.17 0.23 0.1 0.1 0.17 0 0 0 0 0.09 0.19
Endoplasmic Reticulum 0.74 0.74 – 0.6 0.8 0.71 0.48 – 0.33 0.26 0.07 0.13 – 0.72 0.58 0 0 0.05 0.84 0.82 0.78 0.57 0.44 0.43
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0.07 0 0 0 – 0 0 0 0 – 0.06 0 0.66 0.52 0.43 0 0 0 0 0.06 0
Mitochondria 0.16 0 – 0 0 0.06 0.08 – 0.29 0.24 0.59 0.39 – 0 0 0.06 0.06 0.05 0 0 0 0.19 0.12 0
Nucleus 0 0 – 0 0 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.15 – 0.18 0.12 0.11 0.4 – 0.31 0.48 0.27 0.38 – 0.17 0.21 0.35 0.43 0.4 0 0 0.07 0 0.13 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 7 – 2 1 3 2 – 1 0 0 0 – 0 0 9 11 8 6 2 0 1 0 0
Bud 1 1 – 0 1 0 1 – 0 1 0 0 – 0 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 – 0 1 3 3 – 0 1 0 0 – 0 0 9 7 5 0 0 0 0 0 0
Cytoplasm 19 21 – 8 11 13 15 – 3 12 5 8 – 3 10 28 20 22 1 3 0 0 4 12
Endoplasmic Reticulum 105 111 – 27 41 57 51 – 16 23 4 10 – 13 25 8 6 6 150 134 16 4 21 28
Endosome 3 0 – 0 0 1 0 – 0 1 1 1 – 1 0 2 6 3 3 3 0 0 2 1
Golgi 3 6 – 3 1 2 1 – 1 1 0 0 – 1 0 185 104 56 2 5 0 0 2 0
Mitochondria 22 3 – 2 0 5 8 – 14 21 35 31 – 0 0 17 11 7 5 5 0 1 5 1
Nucleus 0 0 – 0 0 0 1 – 3 4 1 3 – 0 1 2 1 0 0 0 0 0 0 1
Nuclear Periphery 8 0 – 0 1 0 0 – 2 3 2 2 – 0 0 4 0 1 0 0 0 0 1 0
Nucleolus 0 0 – 1 0 0 0 – 0 0 0 0 – 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 13 22 – 8 6 9 42 – 15 43 16 30 – 3 9 100 86 52 7 6 1 0 6 14
Unique Cell Count 141 150 45 51 80 106 48 89 59 79 18 43 282 200 129 180 165 21 8 49 66
Labelled Cell Count 180 174 51 63 93 124 55 110 64 85 21 45 365 252 163 180 165 21 8 49 66


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 11.3 – 9.0 10.3 8.6 8.7 – 7.6 7.7 7.2 7.4 – 11.4 10.9 18.2 16.9 16.3 – – 9.9
Std Deviation (1e-4) 1.6 2.1 – 1.3 1.3 1.3 1.4 – 1.2 1.3 1.2 1.2 – 1.2 1.5 4.5 3.4 4.7 – – 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0432 6.1161 5.6453 5.0814 4.2418 5.5561 6.3782 6.7447 6.6001 5.9523 5.0457 6.3626 5.1991 5.61 5.1644 5.1263 3.949 5.5572
Actin 0.0055 0.0012 0.0043 0.0044 0.0058 0.0024 0.0137 0.0013 0.0074 0.0051 0.0033 0.0122 0.0198 0.0016 0.0215 0.0012 0.0582 0.001
Bud 0.0005 0.0005 0.0018 0.0007 0.001 0.0007 0.0003 0.0001 0.0002 0.0003 0.0002 0.0004 0.0002 0.0001 0.0006 0.0002 0.0008 0.0001
Bud Neck 0.0006 0.0005 0.0004 0.0014 0.0014 0.0011 0.0005 0.0002 0.0005 0.0008 0.0004 0.0149 0.0008 0.0006 0.0007 0.0009 0.0019 0.0005
Bud Periphery 0.0007 0.0007 0.0014 0.0007 0.0018 0.0006 0.0006 0.0001 0.0004 0.0008 0.0004 0.001 0.0005 0.0002 0.0005 0.0008 0.0011 0.0001
Bud Site 0.0013 0.008 0.0019 0.0038 0.0025 0.0004 0.0014 0.0007 0.0027 0.0014 0.0003 0.0004 0.0009 0.0017 0.002 0.0002 0.0024 0.0001
Cell Periphery 0.001 0.0016 0.0011 0.0006 0.0008 0.0009 0.0008 0.0008 0.0009 0.001 0.0005 0.0015 0.0008 0.0012 0.0007 0.0014 0.0007 0.0005
Cytoplasm 0.0306 0.0404 0.0147 0.0083 0.0165 0.0196 0.0257 0.0556 0.0481 0.0233 0.0256 0.047 0.0236 0.0467 0.0421 0.0095 0.0338 0.035
Cytoplasmic Foci 0.033 0.0112 0.0174 0.014 0.0419 0.0195 0.0382 0.0244 0.0174 0.0277 0.0308 0.0182 0.0186 0.0191 0.0244 0.0033 0.0387 0.0101
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0002 0.0003 0 0.0005 0.0001 0.0005 0
Endoplasmic Reticulum 0.4249 0.6621 0.6369 0.6172 0.3271 0.6133 0.5392 0.6463 0.6607 0.5739 0.5732 0.6078 0.562 0.6332 0.674 0.7724 0.4984 0.6237
Endosome 0.1232 0.0805 0.1163 0.1104 0.1648 0.0864 0.0878 0.1076 0.0945 0.1177 0.135 0.0821 0.0959 0.1169 0.0831 0.0782 0.1003 0.1014
Golgi 0.023 0.0135 0.0328 0.0509 0.083 0.0252 0.0275 0.015 0.0268 0.0511 0.07 0.0265 0.0145 0.0186 0.0233 0.0141 0.0453 0.0204
Lipid Particles 0.0903 0.0732 0.0592 0.0501 0.0935 0.0815 0.0862 0.0766 0.0653 0.1141 0.0653 0.0322 0.0672 0.0794 0.0374 0.0159 0.0656 0.061
Mitochondria 0.0296 0.0068 0.0267 0.0299 0.1481 0.0144 0.0093 0.0032 0.0054 0.0066 0.0134 0.0227 0.0132 0.003 0.0123 0.0096 0.024 0.005
None 0.0211 0.0022 0.0013 0.0004 0.002 0.0048 0.0083 0.0011 0.0008 0.0016 0.0008 0.0007 0.0076 0.0007 0.0022 0.0005 0.0024 0.0006
Nuclear Periphery 0.1247 0.0502 0.0468 0.0344 0.023 0.0731 0.0879 0.044 0.046 0.0195 0.0416 0.1005 0.1165 0.0455 0.0473 0.0653 0.0481 0.1089
Nucleolus 0.0052 0.0011 0.0016 0.0006 0.004 0.0036 0.0007 0.0001 0.0001 0.0008 0.0001 0.0002 0.0006 0.0005 0.0003 0 0.0014 0.0001
Nucleus 0.0137 0.016 0.0035 0.0467 0.0149 0.0132 0.0137 0.0021 0.002 0.0017 0.0012 0.0035 0.0044 0.003 0.002 0.0018 0.006 0.0055
Peroxisomes 0.0176 0.0006 0.0013 0.0027 0.011 0.0013 0.0088 0.0007 0.001 0.0153 0.0214 0.0028 0.017 0.0006 0.006 0.0001 0.0064 0.0002
Punctate Nuclear 0.015 0.0022 0.0021 0.0052 0.0224 0.0107 0.0289 0.0012 0.0024 0.0117 0.0009 0.0016 0.0048 0.0021 0.0036 0.0002 0.026 0.0011
Vacuole 0.0232 0.0216 0.0206 0.011 0.0233 0.0168 0.0149 0.0153 0.0118 0.0187 0.0071 0.0153 0.0198 0.0208 0.0096 0.015 0.0293 0.0159
Vacuole Periphery 0.015 0.0058 0.0076 0.0064 0.0106 0.0104 0.0056 0.0035 0.0056 0.0068 0.0082 0.0085 0.0109 0.0044 0.0057 0.0092 0.009 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.9781 16.2991 21.6869 20.5605 22.0094 36.4456 25.5724 23.0129 24.8259 25.2031
Translational Efficiency 0.7705 1.2101 0.9365 1.0976 0.8677 0.6493 0.7188 0.9542 0.9432 0.8495

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
Localization
Cell Percentages ER (92%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ubx2

Ubx2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubx2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available