Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0.06 0.07 0.05 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.1 0.13 0.1 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.09 0.06 0 0.15 0.17 0.13 0.21 0.24 0.24 0.2 0 0 0 0.09 0.05 0.05 0 0 0 0 0 0
Nucleus 0.92 0.92 0.91 0.89 0.87 0.83 0.79 0.73 0.74 0.71 0.77 0.76 0.84 0.83 0.81 0.76 0.81 0.79 0.87 0.88 0.85 0.86 0.74 0.66
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.09 0.1 0.1 0.12 0.16 0.21 0.29 0.28 0.33 0.31 0.33 0.05 0.11 0.06 0.07 0.06 0.06 0.06 0 0.05 0.05 0.08 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 0 2 0 4 1 2 0 0 0 0 0 0 1 4 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 23 16 9 22 25 22 38 3 1 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 4 2 3 15 5 11 12 9 3 6 3 17 43 49 23 11 6 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 1 1 0 0 1 0 1 0 0 0 1 0 0 8 5 2 0 0 0 0 0 1
Endosome 1 2 1 2 3 0 1 3 0 0 0 1 1 2 1 10 5 5 0 3 2 0 5 3
Golgi 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2
Mitochondria 26 9 23 14 11 55 72 74 80 93 96 108 2 0 2 22 6 4 0 2 1 0 3 7
Nucleus 171 313 235 210 257 311 337 414 275 272 313 411 185 347 301 176 97 67 163 310 231 100 211 164
Nuclear Periphery 2 12 3 5 5 8 21 11 14 8 9 10 5 7 7 4 3 0 0 0 2 0 0 3
Nucleolus 20 30 27 24 36 61 89 166 105 127 126 178 12 45 22 17 7 5 11 7 13 5 23 19
Peroxisomes 0 0 0 0 1 2 0 0 0 0 1 1 3 7 8 5 1 0 0 0 0 0 0 0
SpindlePole 1 4 0 3 1 4 2 7 1 1 0 2 4 7 5 8 3 1 1 11 4 1 3 2
Vac/Vac Membrane 0 5 0 0 0 3 4 8 0 0 1 3 3 4 4 5 1 2 6 10 14 4 25 34
Unique Cell Count 185 339 259 235 297 376 426 566 374 382 405 544 219 419 372 232 120 85 188 353 272 117 285 248
Labelled Cell Count 225 379 292 264 331 474 554 706 507 534 576 759 236 463 399 279 140 92 188 353 272 117 285 248


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.8 6.2 6.5 7.0 6.4 5.9 6.1 5.9 5.5 5.6 5.5 8.1 7.9 7.6 7.2 8.2 8.8 6.4 6.6 7.2
Std Deviation (1e-4) 1.6 1.3 1.2 1.9 1.9 1.7 1.8 1.7 1.6 1.4 1.6 1.5 2.3 2.4 2.5 1.9 2.1 1.9 2.0 1.7 1.5
Intensity Change (Log2) – – – 0.07 0.17 0.05 -0.08 -0.03 -0.06 -0.18 -0.15 -0.17 0.39 0.35 0.3 0.22 0.42 0.52 0.05 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 4.1 3.2 0 4.0 4.2 3.8 4.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.9 0 0 0 0 0 0 0 3.9 4.8 5.6 4.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.2 -2.5 2.2 2.9 1.7 4.2 5.0 4.9 3.9 -3.9 -6.2 -5.3 0.2 -1.3 -1.2
Nucleus -0.5 -1.5 -2.9 -4.0 -5.7 -5.4 -6.0 -4.5 -5.1 -2.1 -2.9 -3.4 -4.5 -2.7 -2.9
Nuclear Periphery 0 0 0 2.6 0 2.0 0 0 0 0 0 0 0 0 0
Nucleolus -0.1 0.6 2.1 3.5 6.0 5.4 6.6 6.2 6.8 -2.0 0.1 -2.1 -1.2 -1.5 -1.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1764 3.2001 2.6329 2.5027 1.7277 2.4237 2.9295 3.8184 3.3282 2.9112 3.0077 3.2679 4.3392 3.4925 3.4171 2.9801 3.5259 3.4884
Actin 0.0018 0.0001 0 0.0001 0 0 0.0148 0.0061 0.005 0.012 0 0.0026 0.1561 0 0.0081 0 0.0001 0.0019
Bud 0.0003 0.0001 0.0001 0.0004 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0047 0 0.0008 0 0.0002 0.0001
Bud Neck 0.0014 0.0002 0.0001 0.0007 0.0009 0.0024 0.0011 0.0002 0.0004 0.0008 0.0002 0.0015 0.0017 0.0001 0.0006 0.0001 0.0011 0.0021
Bud Periphery 0.0005 0.0001 0.0001 0.0002 0.0002 0.0002 0.0004 0 0.0005 0.0002 0 0.0002 0.0073 0 0.0006 0 0.0005 0.0003
Bud Site 0.0023 0.0005 0.0002 0.0003 0.0002 0.0001 0.0017 0.0001 0.0002 0.0014 0 0.0001 0.0064 0.0001 0.0049 0 0.0013 0.0002
Cell Periphery 0.0003 0 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0013 0 0.0002 0 0.0003 0.0001
Cytoplasm 0.0085 0.0211 0.0002 0.0066 0.0001 0.0001 0.003 0.0002 0.0003 0.0012 0 0.0001 0.042 0.0001 0.0236 0.0002 0.0002 0.0002
Cytoplasmic Foci 0.0098 0.009 0 0.0127 0 0 0.0085 0.0006 0.0018 0.0188 0.0001 0.0004 0.0097 0 0.0065 0.0001 0.0001 0.0009
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0004 0.0001 0 0 0.001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.005 0.0001 0.0001 0.0001 0 0 0.0017 0 0.0003 0.0009 0 0.0001 0.0269 0 0.0029 0 0.0001 0.0003
Endosome 0.043 0.0009 0.0003 0.0002 0 0.0001 0.0116 0.0003 0.0015 0.0142 0 0.0009 0.0743 0 0.0145 0 0.0003 0.0035
Golgi 0.0035 0.0004 0 0 0 0 0.0038 0.0021 0.0006 0.0047 0 0.0017 0.0266 0 0.0155 0 0 0.0098
Lipid Particles 0.028 0.0078 0 0.0003 0 0 0.0215 0.0001 0.0027 0.023 0 0.0045 0.0146 0 0.0021 0 0.0001 0.0002
Mitochondria 0.0058 0.0003 0.0003 0.0005 0.0005 0.0001 0.0041 0.0006 0.0044 0.0013 0.0002 0.0035 0.0797 0.0001 0.004 0 0.0008 0.0137
None 0.0038 0.0001 0 0.001 0.0001 0 0.0015 0 0.0002 0.0012 0 0.0001 0.0404 0 0.0056 0 0.0002 0.0001
Nuclear Periphery 0.0075 0.0018 0.0027 0.005 0.001 0.0006 0.005 0.0026 0.0065 0.0136 0.0071 0.0052 0.1074 0.0006 0.024 0.0022 0.0015 0.0044
Nucleolus 0.039 0.0487 0.0354 0.07 0.11 0.0861 0.0353 0.0264 0.0343 0.0226 0.0784 0.0776 0.0467 0.014 0.0221 0.0174 0.2012 0.1061
Nucleus 0.8228 0.8822 0.8857 0.8759 0.8855 0.9081 0.8605 0.9166 0.8856 0.7321 0.8414 0.897 0.2891 0.933 0.8106 0.6952 0.6206 0.8366
Peroxisomes 0.0049 0.0004 0 0 0 0 0.0095 0.0001 0.0005 0.0096 0 0.0009 0.012 0 0.0012 0 0.0001 0.0002
Punctate Nuclear 0.0039 0.0261 0.0696 0.0246 0.001 0.0015 0.0133 0.0437 0.0522 0.14 0.0722 0.0031 0.0121 0.0518 0.0498 0.2845 0.1699 0.0186
Vacuole 0.0064 0.0002 0.0037 0.0007 0.0002 0.0004 0.0014 0 0.0012 0.0013 0 0.0002 0.0272 0.0001 0.0012 0 0.0012 0.0004
Vacuole Periphery 0.0015 0.0001 0.0014 0.0003 0.0001 0.0001 0.0007 0 0.001 0.0009 0.0002 0.0002 0.0129 0 0.0009 0 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.5592 27.8637 27.3958 30.1384 18.1857 31.9312 30.6381 33.0383 35.2359 29.5384
Translational Efficiency 0.6832 0.8096 0.6963 0.6633 0.911 0.7183 0.768 0.5515 0.6091 0.607

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rad52

Rad52


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad52-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available