Standard name | |
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Human Ortholog | |
Description | GTP-binding protein of the Ras superfamily; involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 6 | 6 | 9 | 3 | 3 | 2 | 0 | 4 | 1 | 4 | 6 | 5 |
Bud | 1 | 5 | 3 | 13 | 13 | 19 | 37 | 36 | 49 | 53 | 43 | 53 | 4 | 2 | 7 | 3 | 5 | 5 | 4 | 8 | 1 | 6 | 9 | 9 |
Bud Neck | 0 | 2 | 0 | 2 | 6 | 10 | 11 | 11 | 10 | 15 | 6 | 13 | 14 | 23 | 23 | 0 | 3 | 0 | 0 | 2 | 0 | 2 | 3 | 3 |
Bud Site | 2 | 0 | 4 | 3 | 4 | 8 | 14 | 15 | 22 | 19 | 12 | 19 | 0 | 3 | 1 | 4 | 6 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Cytoplasm | 13 | 71 | 34 | 124 | 220 | 207 | 246 | 228 | 179 | 249 | 155 | 207 | 175 | 361 | 392 | 49 | 56 | 47 | 15 | 58 | 23 | 87 | 254 | 282 |
Endoplasmic Reticulum | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 6 | 7 | 4 | 5 | 0 | 2 | 0 | 0 | 4 | 3 |
Endosome | 2 | 31 | 6 | 26 | 18 | 0 | 1 | 4 | 0 | 6 | 4 | 10 | 5 | 14 | 25 | 50 | 82 | 56 | 2 | 16 | 6 | 2 | 6 | 14 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 3 |
Mitochondria | 8 | 3 | 19 | 21 | 52 | 92 | 204 | 167 | 186 | 224 | 147 | 262 | 1 | 1 | 0 | 8 | 3 | 1 | 2 | 6 | 3 | 12 | 28 | 29 |
Nucleus | 0 | 6 | 1 | 0 | 1 | 2 | 3 | 4 | 3 | 1 | 3 | 1 | 11 | 1 | 1 | 3 | 2 | 1 | 1 | 9 | 5 | 8 | 6 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 0 | 0 | 0 | 3 | 8 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 7 | 2 | 2 | 1 | 3 | 2 | 7 | 10 | 10 | 0 | 1 | 1 | 4 | 9 | 9 | 1 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 13 | 16 | 19 | 54 | 45 | 21 | 78 | 77 | 72 | 133 | 66 | 107 | 16 | 58 | 77 | 85 | 105 | 74 | 40 | 56 | 55 | 23 | 24 | 18 |
Vac/Vac Membrane | 1 | 22 | 1 | 3 | 3 | 2 | 1 | 3 | 3 | 6 | 2 | 4 | 13 | 7 | 4 | 16 | 24 | 16 | 1 | 9 | 3 | 15 | 26 | 11 |
Unique Cell Count | 33 | 147 | 73 | 214 | 315 | 303 | 454 | 419 | 380 | 547 | 337 | 533 | 226 | 459 | 508 | 176 | 219 | 164 | 73 | 184 | 107 | 172 | 384 | 401 |
Labelled Cell Count | 43 | 167 | 88 | 257 | 365 | 364 | 597 | 550 | 530 | 720 | 450 | 693 | 247 | 481 | 548 | 235 | 310 | 222 | 73 | 184 | 107 | 172 | 384 | 401 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.4 | 3.7 | 3.5 | 3.4 | 3.2 | 2.9 | 3.2 | 2.9 | 2.9 | 2.8 | 2.8 | 5.0 | 4.9 | 5.0 | 6.9 | 6.8 | 7.2 | 5.5 | 5.2 | 5.3 |
Std Deviation (1e-4) | 2.6 | 1.3 | 0.7 | 1.3 | 0.9 | 1.3 | 1.3 | 1.4 | 0.7 | 0.9 | 0.9 | 0.7 | 1.0 | 1.1 | 0.7 | 1.4 | 1.3 | 1.7 | 1.7 | 1.2 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.14 | -0.19 | -0.37 | -0.22 | -0.37 | -0.34 | -0.39 | -0.38 | 0.45 | 0.41 | 0.44 | 0.9 | 0.87 | 0.96 | 0.58 | 0.5 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 3.8 | 3.5 | 1.2 | 1.2 | 0.1 | -0.2 | -0.1 | -1.3 | 5.0 | 5.8 | 5.5 | -2.9 | -3.4 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 4.7 | 4.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.0 | -6.9 | -6.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.4 | -3.0 | -2.3 | 3.2 | 3.3 | 2.8 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0473 | 0.0675 | 0.1726 | 0.2759 | 0.4465 | 0.3633 | 0.7015 | 1.3665 | 1.0869 | 1.336 | 1.381 | 1.1856 | -2.1958 | -0.6434 | -1.1173 | -0.7536 | -0.8229 | -0.8103 |
Actin | 0.0338 | 0.0005 | 0.0116 | 0.0406 | 0.0001 | 0.0063 | 0.0169 | 0.0102 | 0.0097 | 0.0063 | 0.0368 | 0.0014 | 0.0049 | 0.0004 | 0.001 | 0.0006 | 0.0002 | 0.007 |
Bud | 0.0014 | 0.0006 | 0.0046 | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.0024 | 0.0002 | 0.0058 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0 | 0 | 0.0003 |
Bud Neck | 0.0054 | 0.0005 | 0.007 | 0.001 | 0.0002 | 0.0022 | 0.0054 | 0.0014 | 0.005 | 0.0319 | 0.0035 | 0.0053 | 0.0002 | 0.0007 | 0.0029 | 0.0028 | 0.0004 | 0.0037 |
Bud Periphery | 0.002 | 0.0008 | 0.0079 | 0.0003 | 0.0001 | 0.0003 | 0.0014 | 0.0005 | 0.0046 | 0.0002 | 0.0077 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0145 | 0.0043 | 0.0132 | 0.0013 | 0.0003 | 0.0009 | 0.0066 | 0.0118 | 0.0128 | 0.001 | 0.0139 | 0.0003 | 0.0006 | 0.0052 | 0.0018 | 0.0002 | 0.0002 | 0.0004 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.074 | 0.1281 | 0.0876 | 0.1224 | 0.09 | 0.16 | 0.0509 | 0.0647 | 0.0536 | 0.0588 | 0.0438 | 0.1263 | 0.1049 | 0.275 | 0.1172 | 0.1056 | 0.0468 | 0.1219 |
Cytoplasmic Foci | 0.1595 | 0.1886 | 0.2216 | 0.3201 | 0.4004 | 0.2036 | 0.1812 | 0.1755 | 0.2693 | 0.3832 | 0.3662 | 0.2329 | 0.2723 | 0.2937 | 0.4177 | 0.5003 | 0.6134 | 0.2204 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0053 | 0.003 | 0.0024 | 0.0063 | 0.0028 | 0.0018 | 0.0067 | 0.0028 | 0.0018 | 0.0014 | 0.0031 | 0.0018 | 0.004 | 0.0037 | 0.004 | 0.0041 | 0.0011 | 0.0015 |
Endosome | 0.036 | 0.0222 | 0.0245 | 0.061 | 0.1034 | 0.0159 | 0.04 | 0.0414 | 0.0253 | 0.0483 | 0.0492 | 0.0192 | 0.0268 | 0.0374 | 0.0335 | 0.0289 | 0.0953 | 0.0187 |
Golgi | 0.0114 | 0.002 | 0.0092 | 0.0208 | 0.0042 | 0.0024 | 0.0085 | 0.0053 | 0.0058 | 0.006 | 0.0252 | 0.0014 | 0.0015 | 0.0017 | 0.0019 | 0.002 | 0.0061 | 0.0017 |
Lipid Particles | 0.0161 | 0.0059 | 0.0075 | 0.0217 | 0.0217 | 0.0047 | 0.025 | 0.0054 | 0.013 | 0.0084 | 0.0443 | 0.0097 | 0.0193 | 0.0128 | 0.0127 | 0.0127 | 0.0356 | 0.0075 |
Mitochondria | 0.008 | 0.0011 | 0.005 | 0.0056 | 0.0016 | 0.0011 | 0.0039 | 0.0031 | 0.0065 | 0.0016 | 0.0128 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0012 | 0.0006 |
None | 0.4866 | 0.5651 | 0.5382 | 0.2649 | 0.2806 | 0.2183 | 0.4896 | 0.4729 | 0.4738 | 0.3551 | 0.2307 | 0.3207 | 0.4961 | 0.255 | 0.3188 | 0.262 | 0.0738 | 0.1752 |
Nuclear Periphery | 0.0268 | 0.0018 | 0.0012 | 0.0031 | 0.0326 | 0.0052 | 0.0104 | 0.0018 | 0.0036 | 0.0012 | 0.0041 | 0.0058 | 0.0038 | 0.0031 | 0.0027 | 0.003 | 0.0005 | 0.01 |
Nucleolus | 0.0011 | 0.0008 | 0.0009 | 0.0004 | 0.0004 | 0.0044 | 0.0037 | 0.0016 | 0.001 | 0.0016 | 0.0013 | 0.0033 | 0.0005 | 0.0009 | 0.0006 | 0.0003 | 0.0002 | 0.004 |
Nucleus | 0.0295 | 0.0106 | 0.0017 | 0.0015 | 0.0052 | 0.0781 | 0.0305 | 0.0194 | 0.0048 | 0.0016 | 0.007 | 0.0649 | 0.0045 | 0.0119 | 0.0035 | 0.0018 | 0.0004 | 0.1161 |
Peroxisomes | 0.0191 | 0.0105 | 0.0099 | 0.0407 | 0.024 | 0.0088 | 0.0306 | 0.0083 | 0.0233 | 0.0235 | 0.0893 | 0.0123 | 0.0074 | 0.0046 | 0.0127 | 0.0267 | 0.0581 | 0.0054 |
Punctate Nuclear | 0.0508 | 0.0509 | 0.044 | 0.0824 | 0.0152 | 0.2831 | 0.083 | 0.1688 | 0.0779 | 0.0668 | 0.0481 | 0.1923 | 0.0486 | 0.0864 | 0.0666 | 0.0469 | 0.0647 | 0.3034 |
Vacuole | 0.0144 | 0.0022 | 0.0012 | 0.0042 | 0.009 | 0.0021 | 0.0026 | 0.0035 | 0.0037 | 0.0021 | 0.0056 | 0.001 | 0.0031 | 0.0053 | 0.0014 | 0.0012 | 0.0016 | 0.0014 |
Vacuole Periphery | 0.0031 | 0.0003 | 0.0004 | 0.0012 | 0.008 | 0.0004 | 0.0012 | 0.0003 | 0.0011 | 0.0004 | 0.0011 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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