Standard name | |
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Human Ortholog | |
Description | Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 1 | 5 | 6 | 10 | 13 | 3 | 13 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 4 | – | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 20 | – | 6 | 14 | 8 | 22 | 41 | 13 | 33 | 23 | 30 | – | 2 | 6 | 36 | 44 | 32 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 181 | 254 | – | 148 | 360 | 255 | 367 | 436 | 227 | 275 | 209 | 182 | – | 56 | 151 | 306 | 411 | 391 | 177 | 252 | 39 | 27 | 75 | 183 |
Endoplasmic Reticulum | 0 | 4 | – | 0 | 2 | 5 | 30 | 58 | 14 | 34 | 15 | 19 | – | 0 | 1 | 7 | 18 | 14 | 0 | 1 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | – | 0 | 0 | 0 | 6 | 4 | 9 | 14 | 26 | 10 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 6 | – | 3 | 2 | 1 | 4 | 5 | 4 | 2 | 0 | 0 | – | 1 | 0 | 0 | 1 | 2 | 0 | 3 | 3 | 3 | 0 | 1 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | – | 1 | 2 | 0 | 8 | 17 | 13 | 40 | 9 | 19 | – | 0 | 0 | 5 | 8 | 13 | 1 | 15 | 4 | 0 | 0 | 1 |
Unique Cell Count | 183 | 268 | 150 | 366 | 265 | 419 | 525 | 272 | 371 | 251 | 242 | 57 | 152 | 319 | 425 | 405 | 183 | 281 | 54 | 37 | 78 | 193 | ||
Labelled Cell Count | 185 | 291 | 158 | 380 | 270 | 445 | 568 | 293 | 413 | 286 | 276 | 60 | 158 | 355 | 482 | 452 | 183 | 281 | 54 | 37 | 78 | 193 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.9 | 19.4 | – | 14.7 | 16.5 | 15.7 | 15.8 | 15.7 | 14.2 | 15.1 | 14.0 | 13.9 | – | 17.4 | 20.4 | 33.3 | 31.5 | 28.1 | – | 18.8 | 18.8 |
Std Deviation (1e-4) | 3.3 | 5.4 | – | 3.1 | 3.6 | 3.3 | 3.4 | 4.0 | 3.9 | 4.3 | 4.3 | 4.1 | – | 5.7 | 5.4 | 13.2 | 10.8 | 10.8 | – | 5.2 | 5.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.7189 | 17.7881 | 16.0639 | 15.8626 | 13.8403 | 14.8278 | 9.25 | 12.5037 | 13.4324 | 11.9316 | 10.8636 | 12.4223 | 7.708 | 11.0648 | 9.9388 | 11.0794 | 7.916 | 10.3009 |
Actin | 0.0122 | 0.0014 | 0.0014 | 0.0002 | 0.0015 | 0.0016 | 0.01 | 0.0096 | 0.0087 | 0.0062 | 0.0305 | 0.0021 | 0.0167 | 0.0005 | 0.0095 | 0.0001 | 0.0582 | 0.0005 |
Bud | 0.0014 | 0.002 | 0.0083 | 0.0004 | 0.0015 | 0.0038 | 0.0004 | 0.0017 | 0.0008 | 0.0018 | 0.001 | 0.0006 | 0.0005 | 0.0002 | 0.0014 | 0.0001 | 0.0042 | 0.0036 |
Bud Neck | 0.001 | 0.0004 | 0.0006 | 0.0005 | 0.0016 | 0.0016 | 0.001 | 0.0003 | 0.0004 | 0.001 | 0.0006 | 0.0018 | 0.0011 | 0.0001 | 0.0005 | 0.0002 | 0.0009 | 0.0006 |
Bud Periphery | 0.0008 | 0.0008 | 0.0018 | 0.0002 | 0.0009 | 0.0022 | 0.0005 | 0.0011 | 0.0011 | 0.0029 | 0.0014 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0014 | 0.001 |
Bud Site | 0.0027 | 0.0043 | 0.0033 | 0.0002 | 0.0016 | 0.0015 | 0.0043 | 0.0043 | 0.0022 | 0.0013 | 0.0043 | 0.0017 | 0.002 | 0.0007 | 0.0009 | 0 | 0.0019 | 0.0002 |
Cell Periphery | 0.001 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.9044 | 0.9732 | 0.958 | 0.9498 | 0.9162 | 0.9433 | 0.7684 | 0.9458 | 0.937 | 0.907 | 0.6232 | 0.8681 | 0.8657 | 0.9768 | 0.9537 | 0.9877 | 0.8081 | 0.972 |
Cytoplasmic Foci | 0.0082 | 0.0012 | 0.0033 | 0.0022 | 0.0174 | 0.0084 | 0.0266 | 0.0062 | 0.0147 | 0.0071 | 0.037 | 0.0306 | 0.0227 | 0.0038 | 0.008 | 0.0023 | 0.0122 | 0.0042 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0137 | 0.0047 | 0.0068 | 0.0078 | 0.0033 | 0.0127 | 0.009 | 0.0015 | 0.002 | 0.0035 | 0.0046 | 0.0015 | 0.0091 | 0.0009 | 0.0019 | 0.0013 | 0.0051 | 0.0038 |
Endosome | 0.0136 | 0.0029 | 0.0035 | 0.0048 | 0.0133 | 0.0075 | 0.034 | 0.0025 | 0.0064 | 0.0222 | 0.0681 | 0.0279 | 0.0274 | 0.0018 | 0.0045 | 0.0024 | 0.0209 | 0.0051 |
Golgi | 0.0022 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0006 | 0.0061 | 0.0012 | 0.0012 | 0.004 | 0.0465 | 0.0111 | 0.0038 | 0.0002 | 0.0016 | 0 | 0.0058 | 0.0002 |
Lipid Particles | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.028 | 0.0007 | 0.0015 | 0.0094 | 0.0128 | 0.0148 | 0.0049 | 0.0001 | 0.0007 | 0 | 0.0148 | 0 |
Mitochondria | 0.0036 | 0.0001 | 0.0008 | 0.0002 | 0.0008 | 0.0014 | 0.0054 | 0.0005 | 0.0021 | 0.0087 | 0.0215 | 0.0109 | 0.0063 | 0.0001 | 0.0005 | 0.0001 | 0.0021 | 0.0006 |
None | 0.008 | 0.0007 | 0.0014 | 0.0007 | 0.0027 | 0.0037 | 0.0141 | 0.018 | 0.0092 | 0.002 | 0.0009 | 0.0008 | 0.0093 | 0.0112 | 0.0106 | 0.0005 | 0.0131 | 0.0024 |
Nuclear Periphery | 0.005 | 0.0014 | 0.0011 | 0.0017 | 0.0028 | 0.0026 | 0.0225 | 0.0009 | 0.0045 | 0.0029 | 0.0107 | 0.0019 | 0.0092 | 0.0005 | 0.0012 | 0.0018 | 0.0105 | 0.0008 |
Nucleolus | 0.0007 | 0 | 0.0003 | 0 | 0.0026 | 0.0001 | 0.008 | 0.0001 | 0.0002 | 0.0031 | 0.0006 | 0.0052 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0002 |
Nucleus | 0.01 | 0.0028 | 0.0041 | 0.0029 | 0.0214 | 0.0045 | 0.013 | 0.0012 | 0.0026 | 0.0046 | 0.0054 | 0.0027 | 0.004 | 0.0013 | 0.0019 | 0.0017 | 0.0112 | 0.0028 |
Peroxisomes | 0.0019 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.031 | 0.0016 | 0.0021 | 0.002 | 0.0674 | 0.0043 | 0.0036 | 0.0001 | 0.0007 | 0 | 0.003 | 0 |
Punctate Nuclear | 0.0017 | 0.0001 | 0.0004 | 0.0001 | 0.0026 | 0.0008 | 0.0027 | 0.0009 | 0.0017 | 0.0054 | 0.0013 | 0.0081 | 0.0084 | 0.0004 | 0.0007 | 0.0001 | 0.0175 | 0.0003 |
Vacuole | 0.004 | 0.0031 | 0.0033 | 0.0268 | 0.0078 | 0.0026 | 0.0112 | 0.0013 | 0.001 | 0.0034 | 0.0395 | 0.0043 | 0.0028 | 0.001 | 0.0012 | 0.0013 | 0.0049 | 0.0011 |
Vacuole Periphery | 0.0009 | 0.0005 | 0.0005 | 0.0014 | 0.0009 | 0.0006 | 0.0029 | 0.0002 | 0.0005 | 0.0012 | 0.0221 | 0.001 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0016 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.0183 | 51.7075 | 51.715 | 64.2517 | 57.0615 | 53.4117 | 65.4938 | 73.0659 | 75.4787 | 74.6066 |
Translational Efficiency | 1.7909 | 2.2202 | 1.8866 | 1.8278 | 2.0504 | 2.7501 | 1.7359 | 1.4342 | 1.7444 | 1.6164 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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