Standard name
Human Ortholog
Description Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.12 0.06 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0.07 0.19 0.24 0.26 0.29 0.28 0.29 0.31 0.32 0.27 0.05 0 0 0.14 0.13 0.09 0 0 0 0 0 0
Nucleus 0.75 0.73 0.33 0.05 0.2 0.12 0.12 0.12 0.1 0.1 0.12 0.13 0.16 0.1 0.11 0.12 0.15 0.2 0.46 0.54 0.15 0.18 0.19 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleolus 0.34 0.44 0.67 0.94 0.74 0.82 0.8 0.81 0.82 0.84 0.81 0.78 0.8 0.79 0.75 0.78 0.71 0.78 0.44 0.39 0.67 0.59 0.58 0.58
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0 0.07 0.08 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0.05 0.14 0.15 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 1 3 1 2 1 0 0 0 0 0 0 0 0 0 4 3 6
Bud Neck 0 0 0 0 0 1 1 1 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 5 10 12 14 13 17 18 0 2 0 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 4 7 8 3 8 1 7 6 4 4 30 42 51 15 15 4 0 0 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 2
Endosome 0 0 0 3 2 2 2 2 4 1 0 1 1 3 7 1 3 0 0 1 2 2 2 6
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 2
Mitochondria 12 8 16 37 57 62 107 92 115 119 149 119 20 5 9 33 23 13 0 0 2 6 9 12
Nucleus 145 90 74 10 48 28 45 40 40 38 53 57 63 41 47 30 27 31 97 74 40 46 65 62
Nuclear Periphery 3 0 4 0 1 0 1 4 0 3 6 3 0 2 0 0 1 0 7 1 21 5 3 11
Nucleolus 65 54 147 182 178 197 295 271 321 322 374 344 326 325 319 188 129 119 92 52 174 153 199 269
Peroxisomes 0 0 0 3 1 3 3 3 4 0 4 2 3 2 2 4 2 1 0 0 1 0 0 1
SpindlePole 0 0 1 7 4 11 12 25 33 16 20 34 4 17 18 1 0 2 0 0 3 1 4 7
Vac/Vac Membrane 5 0 4 0 3 2 1 2 1 2 2 5 4 3 10 9 9 6 11 6 11 36 51 77
Unique Cell Count 194 123 221 194 240 240 370 334 393 385 460 439 406 410 427 241 182 152 212 137 260 261 344 466
Labelled Cell Count 231 153 250 249 305 315 485 456 542 522 632 588 451 445 463 281 213 177 212 137 260 261 344 466


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 22.7 21.5 21.4 18.7 16.2 15.3 14.2 14.0 13.7 12.8 13.3 26.9 25.5 23.9 41.6 37.4 44.3 25.8 28.7 28.4
Std Deviation (1e-4) 4.9 6.2 6.7 9.3 6.5 6.6 5.8 4.9 5.7 5.4 4.1 5.2 12.4 13.8 12.7 19.4 17.6 16.2 9.7 10.0 9.0
Intensity Change (Log2) – – – -0.01 -0.21 -0.41 -0.49 -0.6 -0.62 -0.65 -0.75 -0.7 0.32 0.25 0.15 0.95 0.8 1.04 0.26 0.42 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.8 0 2.9 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.0 0 0 0 0 0 0 0 2.9 3.9 4.4 2.4 3.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 4.9 5.3 6.3 5.9 6.4 6.7 7.2 6.0 -1.2 -4.0 -3.2 2.3 1.8 0.5
Nucleus -7.2 -3.3 -5.6 -6.3 -6.1 -7.1 -7.2 -6.9 -6.2 -5.2 -7.3 -7.0 -5.4 -4.3 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.8 1.8 3.8 3.6 3.9 4.2 4.8 4.3 3.3 3.8 3.5 2.2 2.8 0.9 2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.8 0 3.8 4.1 2.7 2.8 3.9 0 2.7 2.7 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.2 1.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.093 23.3629 23.3353 22.2336 19.8082 21.9438 20.6015 26.532 23.5402 22.7472 21.0512 23.0394 25.3298 26.1324 23.6873 20.7669 23.7979 22.4826
Actin 0.0001 0 0 0.0001 0.0001 0.0001 0.0112 0.0002 0.0088 0.0232 0.0521 0.0015 0.015 0.0001 0 0.0001 0.0001 0.0001
Bud 0 0 0.0001 0 0 0 0.0004 0 0.0003 0.0005 0.0008 0.0001 0.0001 0 0 0 0 0
Bud Neck 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0 0 0.0001 0.0002
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0004 0.0004 0.0009 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003
Bud Site 0.0001 0 0.0001 0 0 0 0.0043 0.0002 0.0039 0.0008 0.0047 0.0001 0.0006 0 0 0 0.0001 0
Cell Periphery 0.0003 0.0003 0.0003 0.0007 0.0005 0.0005 0.0012 0.0006 0.0004 0.0004 0.0002 0.0015 0.0006 0.0003 0.0003 0.0001 0.0006 0.0011
Cytoplasm 0 0 0 0 0 0 0.0105 0 0.0004 0.0015 0.0005 0.0001 0.0006 0 0 0 0 0
Cytoplasmic Foci 0.0011 0.0001 0.0002 0.0001 0.0001 0.0002 0.0416 0.0002 0.0038 0.0139 0.0051 0.0041 0.0085 0.0005 0.0001 0 0 0.0001
Eisosomes 0 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0004 0.0002 0.0001 0.0003 0.0001 0 0 0 0.0001
Endoplasmic Reticulum 0 0 0 0 0 0 0.0024 0 0.0008 0.0002 0.0002 0.0006 0.0001 0 0 0 0 0
Endosome 0.0001 0 0.0001 0 0.0001 0.0001 0.0162 0.0002 0.005 0.0084 0.005 0.01 0.0033 0.0001 0 0 0 0.0001
Golgi 0.0001 0 0 0.0001 0.0001 0.0001 0.0039 0.0002 0.0058 0.0216 0.0132 0.0127 0.0014 0.0002 0.0001 0 0 0.0001
Lipid Particles 0.0076 0.0006 0.0014 0.003 0.011 0.0081 0.0391 0.0013 0.0037 0.0033 0.0016 0.0064 0.0094 0.0114 0.0047 0.0001 0.0006 0.0056
Mitochondria 0.0028 0.0018 0.0008 0.0014 0.0068 0.002 0.0184 0.0063 0.0037 0.0056 0.0051 0.0119 0.0096 0.0011 0.0008 0.0015 0.0029 0.0077
None 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0053 0 0.0004 0.0021 0.0002 0.0001 0.001 0.0001 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0266 0.0453 0.0329 0.062 0.0898 0.0496 0.0251 0.0041 0.0104 0.0035 0.0235 0.0411 0.0236 0.0144 0.0372 0.0064 0.028 0.0574
Nucleolus 0.8252 0.7224 0.8583 0.7979 0.692 0.7812 0.6088 0.6967 0.8093 0.8037 0.6482 0.6972 0.785 0.738 0.8137 0.767 0.8188 0.7672
Nucleus 0.121 0.2199 0.0983 0.1045 0.1569 0.1297 0.1449 0.2773 0.1332 0.1035 0.2298 0.179 0.1244 0.226 0.1313 0.2229 0.1408 0.1387
Peroxisomes 0.0002 0.0001 0 0 0.0001 0 0.0196 0.0008 0.003 0.0021 0.0031 0.0007 0.0027 0 0 0 0 0.0001
Punctate Nuclear 0.0011 0.0007 0.0006 0.0011 0.0032 0.0013 0.0193 0.0003 0.0006 0.0016 0.0005 0.0004 0.0033 0.0013 0.0015 0.0005 0.0035 0.0009
Vacuole 0.0011 0.0008 0.0006 0.0014 0.0013 0.0024 0.0049 0.0017 0.0013 0.0006 0.0005 0.0048 0.0012 0.0006 0.0007 0.0001 0.0009 0.0018
Vacuole Periphery 0.0123 0.0076 0.0059 0.0273 0.0375 0.0241 0.0222 0.0097 0.0045 0.0028 0.0043 0.027 0.009 0.0056 0.0091 0.0009 0.0034 0.0185

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 202.2015 217.0666 250.4919 207.7664 244.2226 240.9015 172.0707 186.5257 202.8317 149.5988
Translational Efficiency 2.3606 2.3036 1.7787 2.7574 1.7865 1.5028 1.8604 1.7218 1.4845 1.91

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (28%), nucleolus (25%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fpr3

Fpr3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fpr3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available