ORF | |
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Human Ortholog | |
Description | Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 1 | 5 | 3 | 2 | 2 | 1 | 10 | 7 | 3 | 6 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 5 | 5 | 3 | 1 | 2 | 0 | 2 | 2 | 10 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 180 | 144 | 157 | 172 | 299 | 254 | 321 | 415 | 278 | 274 | 165 | 109 | 170 | 304 | 303 | 199 | 74 | 85 | 174 | 138 | 149 | 134 | 283 | 246 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 8 | 2 | 7 | 0 | 0 | 4 | 0 | 2 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 6 | 2 | 4 | 12 | 16 | 65 | 117 | 178 | 162 | 183 | 96 | 75 | 0 | 0 | 0 | 6 | 4 | 1 | 0 | 1 | 2 | 1 | 3 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 1 | 4 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 2 | 12 | 2 | 1 | 5 |
Unique Cell Count | 183 | 145 | 159 | 175 | 308 | 272 | 363 | 503 | 368 | 378 | 213 | 149 | 171 | 305 | 305 | 207 | 77 | 88 | 179 | 144 | 177 | 146 | 302 | 276 |
Labelled Cell Count | 188 | 149 | 167 | 187 | 322 | 328 | 450 | 618 | 463 | 471 | 272 | 191 | 174 | 307 | 307 | 232 | 87 | 97 | 179 | 144 | 177 | 146 | 302 | 276 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.6 | 4.9 | 4.5 | 5.0 | 4.0 | 3.6 | 3.6 | 3.5 | 3.2 | 3.2 | 3.3 | 6.1 | 6.0 | 5.9 | 7.2 | 7.8 | 9.0 | 6.4 | 6.1 | 6.7 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.8 | 0.8 | 1.8 | 1.3 | 1.4 | 1.1 | 1.0 | 1.1 | 0.7 | 1.5 | 0.9 | 1.1 | 1.2 | 1.2 | 1.5 | 1.5 | 1.7 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.03 | -0.28 | -0.45 | -0.42 | -0.48 | -0.61 | -0.61 | -0.58 | 0.31 | 0.3 | 0.28 | 0.55 | 0.68 | 0.88 | 0.4 | 0.32 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -1.1 | -2.6 | -3.9 | -5.2 | -6.4 | -7.0 | -6.0 | -6.5 | 0.6 | 1.2 | 0.7 | -1.5 | -1.3 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.015 | 0.001 | 0.0212 | 0.0012 | 0.0012 | 0.0051 | 0.064 | 0.0032 | 0.0124 | 0.0011 | 0.0217 | 0.0161 | 0 | 0.0008 | 0.0222 | 0.0001 | 0.0006 | 0.0003 |
Bud | 0.0052 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0013 | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.001 | 0.0004 | 0.0022 | 0.0003 | 0.0003 | 0.001 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0006 | 0 | 0.0001 | 0.0098 | 0.0002 | 0.0003 | 0.0005 |
Bud Periphery | 0.0131 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0019 | 0.0023 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Bud Site | 0.0026 | 0.006 | 0.0056 | 0.0004 | 0.0001 | 0.0003 | 0.0006 | 0.0011 | 0.0069 | 0.0002 | 0.0031 | 0.0001 | 0 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.5462 | 0.7904 | 0.7913 | 0.8211 | 0.6515 | 0.8084 | 0.4843 | 0.6495 | 0.5496 | 0.713 | 0.5218 | 0.7832 | 0.9398 | 0.7554 | 0.6613 | 0.956 | 0.9852 | 0.928 |
Cytoplasmic Foci | 0.0267 | 0.0059 | 0.0093 | 0.0122 | 0.0088 | 0.0094 | 0.0111 | 0.0305 | 0.0392 | 0.0143 | 0.0451 | 0.0038 | 0.0012 | 0.0018 | 0.0182 | 0.0022 | 0.0023 | 0.0044 |
Eisosomes | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0041 | 0.0065 | 0.0027 | 0.0017 | 0.0057 | 0.0064 | 0.0032 | 0.0049 | 0.0056 | 0.0049 | 0.0021 | 0.0011 | 0.0136 | 0.0053 | 0.0007 | 0.0006 | 0.0018 |
Endosome | 0.0261 | 0.0014 | 0.0032 | 0.0144 | 0.0016 | 0.0058 | 0.0271 | 0.0155 | 0.0247 | 0.012 | 0.0471 | 0.0015 | 0.0002 | 0.0006 | 0.0141 | 0.0005 | 0.0004 | 0.0013 |
Golgi | 0.0035 | 0.0003 | 0.0011 | 0.0019 | 0.0003 | 0.003 | 0.0078 | 0.0052 | 0.0149 | 0.0038 | 0.0201 | 0.0002 | 0 | 0.0002 | 0.0431 | 0 | 0.0001 | 0.0001 |
Lipid Particles | 0.0044 | 0.0003 | 0.0011 | 0.0098 | 0.0004 | 0.002 | 0.0344 | 0.0039 | 0.0069 | 0.0001 | 0.0078 | 0.0001 | 0 | 0.0004 | 0.008 | 0 | 0 | 0.0001 |
Mitochondria | 0.003 | 0.0001 | 0.0003 | 0.001 | 0.0002 | 0.003 | 0.0015 | 0.0009 | 0.0164 | 0.0011 | 0.0257 | 0.0002 | 0 | 0 | 0.0171 | 0.0001 | 0 | 0.0001 |
None | 0.3141 | 0.1852 | 0.1481 | 0.124 | 0.3313 | 0.1508 | 0.3442 | 0.2818 | 0.2957 | 0.2428 | 0.26 | 0.187 | 0.0574 | 0.2241 | 0.1475 | 0.0367 | 0.0096 | 0.0625 |
Nuclear Periphery | 0.0019 | 0.0006 | 0.0015 | 0.0013 | 0.0003 | 0.0006 | 0.0046 | 0.0006 | 0.001 | 0.0012 | 0.0009 | 0.0003 | 0.0001 | 0.0009 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Nucleolus | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0022 | 0.0011 | 0.0012 | 0.0013 | 0.0007 | 0.001 | 0.0013 | 0.0007 | 0.001 | 0.0013 | 0.0007 | 0.0005 | 0.0001 | 0.0006 | 0.0005 | 0.0019 | 0.0004 | 0.0004 |
Peroxisomes | 0.001 | 0.0001 | 0.0028 | 0.0027 | 0.0003 | 0.001 | 0.0012 | 0.0014 | 0.0189 | 0.0002 | 0.032 | 0 | 0 | 0 | 0.0494 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0205 | 0.0011 | 0.0011 | 0.0028 | 0.0005 | 0.001 | 0.0012 | 0.0005 | 0.0013 | 0.0005 | 0.0009 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Vacuole | 0.0062 | 0.001 | 0.0017 | 0.002 | 0.0005 | 0.0008 | 0.0042 | 0.0009 | 0.0025 | 0.0015 | 0.0034 | 0.0006 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0019 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0039 | 0.0002 | 0.0004 | 0.0004 | 0.0007 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.9773 | 11.2514 | 12.0597 | 14.7001 | 17.2254 | 16.0737 | 9.7963 | 7.6782 | 10.9106 | 17.8069 |
Translational Efficiency | 0.7265 | 0.8012 | 0.6655 | 0.5397 | 0.5833 | 0.8127 | 0.8721 | 0.6374 | 0.5581 | 0.6303 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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