Standard name | |
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Human Ortholog | |
Description | Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 3 | 2 | 3 | 0 | 2 | 7 | 11 | 19 | 15 | 13 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 5 | 1 | 10 | 2 | 7 | 6 | 9 | 9 | 11 | 4 | 5 | 5 | 4 | 4 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 125 | 225 | 299 | 163 | 279 | 336 | 319 | 403 | 266 | 355 | 278 | 342 | 431 | 685 | 685 | 133 | 187 | 206 | 116 | 215 | 285 | 361 | 584 | 531 |
Endoplasmic Reticulum | 1 | 1 | 2 | 3 | 1 | 2 | 1 | 2 | 3 | 3 | 0 | 3 | 4 | 2 | 3 | 9 | 8 | 3 | 0 | 1 | 3 | 1 | 3 | 0 |
Endosome | 0 | 0 | 2 | 3 | 3 | 1 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 4 | 1 | 0 | 4 | 2 | 8 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 |
Mitochondria | 5 | 2 | 5 | 15 | 31 | 120 | 151 | 197 | 218 | 259 | 235 | 312 | 2 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 4 | 1 | 3 | 1 |
Nucleus | 2 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 1 | 7 | 2 | 6 | 2 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 2 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 7 | 10 | 10 | 8 | 22 | 33 | 42 | 33 | 31 | 41 | 1 | 8 | 8 | 2 | 5 | 5 | 1 | 1 | 4 | 3 | 5 | 1 |
Unique Cell Count | 128 | 228 | 309 | 183 | 314 | 400 | 415 | 551 | 426 | 545 | 461 | 570 | 439 | 695 | 695 | 137 | 197 | 214 | 129 | 223 | 311 | 381 | 622 | 552 |
Labelled Cell Count | 133 | 228 | 324 | 201 | 329 | 479 | 504 | 657 | 550 | 688 | 576 | 730 | 445 | 706 | 707 | 150 | 215 | 237 | 129 | 223 | 311 | 381 | 622 | 552 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 10.5 | 8.3 | 7.6 | 7.0 | 5.8 | 5.5 | 5.4 | 4.9 | 5.0 | 4.5 | 4.5 | 9.5 | 9.4 | 9.4 | 12.2 | 11.8 | 12.2 | 9.6 | 9.4 | 10.2 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.2 | 1.3 | 1.7 | 1.4 | 1.6 | 1.3 | 0.9 | 1.2 | 1.2 | 0.9 | 1.2 | 1.1 | 1.1 | 2.3 | 2.2 | 2.4 | 1.3 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.25 | -0.51 | -0.6 | -0.62 | -0.77 | -0.74 | -0.9 | -0.89 | 0.19 | 0.17 | 0.17 | 0.55 | 0.5 | 0.56 | 0.21 | 0.18 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
Cytoplasm | -3.4 | -3.8 | -5.5 | -7.5 | -8.6 | -10.9 | -10.5 | -11.4 | -11.7 | 1.2 | 1.9 | 1.9 | 0.2 | -1.0 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.6 | 4.4 | 9.8 | 11.3 | 11.3 | 14.4 | 13.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.9 | 0.7 | -0.2 | 2.1 | 2.5 | 4.1 | 2.5 | 2.8 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8644 | 7.5249 | 6.9591 | 6.3659 | 6.2142 | 6.7904 | 3.7492 | 5.409 | 4.8811 | 4.4559 | 4.3324 | 4.7785 | 3.4293 | 5.2512 | 4.4774 | 4.1491 | 3.9857 | 4.3167 |
Actin | 0.0081 | 0.0002 | 0.0049 | 0.0036 | 0.0007 | 0.0013 | 0.0143 | 0.0003 | 0.0105 | 0.0393 | 0.0302 | 0.0029 | 0.0309 | 0.0002 | 0.0004 | 0.0006 | 0.022 | 0.0095 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0017 | 0.0001 | 0.0001 | 0.0004 | 0.0056 | 0.0001 |
Bud Neck | 0.0069 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.001 | 0.0019 | 0.0001 | 0.0004 | 0.0006 | 0.0007 | 0.0007 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0005 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0003 | 0.0005 | 0.0008 | 0.0001 | 0.0016 | 0 | 0 | 0.0004 | 0.0039 | 0.0001 |
Bud Site | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0016 | 0.0003 | 0.0017 | 0.0008 | 0.0025 | 0.0001 | 0.0029 | 0.0001 | 0.0002 | 0.0002 | 0.0055 | 0.0002 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 |
Cytoplasm | 0.8688 | 0.972 | 0.9541 | 0.9241 | 0.9433 | 0.9646 | 0.7882 | 0.9809 | 0.9219 | 0.8213 | 0.6538 | 0.9555 | 0.7715 | 0.9839 | 0.9832 | 0.8544 | 0.8352 | 0.9479 |
Cytoplasmic Foci | 0.0188 | 0.0066 | 0.0102 | 0.0122 | 0.0086 | 0.009 | 0.0389 | 0.0027 | 0.0159 | 0.0477 | 0.0455 | 0.0087 | 0.0503 | 0.006 | 0.0042 | 0.0504 | 0.0214 | 0.0082 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0052 | 0.0011 | 0.0022 | 0.0014 | 0.0017 | 0.0031 | 0.0098 | 0.0026 | 0.0021 | 0.0014 | 0.0028 | 0.0017 | 0.0134 | 0.001 | 0.0014 | 0.0026 | 0.0012 | 0.0026 |
Endosome | 0.0109 | 0.0028 | 0.0053 | 0.0067 | 0.0045 | 0.0077 | 0.0458 | 0.0039 | 0.0155 | 0.0265 | 0.0735 | 0.0044 | 0.0416 | 0.001 | 0.0039 | 0.015 | 0.0269 | 0.0066 |
Golgi | 0.0026 | 0.0002 | 0.0007 | 0.001 | 0.0002 | 0.0004 | 0.0101 | 0.0004 | 0.0019 | 0.0154 | 0.0338 | 0.0012 | 0.0138 | 0.0002 | 0.0002 | 0.0019 | 0.0502 | 0.0013 |
Lipid Particles | 0.0027 | 0 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0042 | 0.0001 | 0.0049 | 0.0101 | 0.0152 | 0.0001 | 0.0118 | 0 | 0 | 0.0008 | 0.004 | 0.0007 |
Mitochondria | 0.0036 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0156 | 0.0001 | 0.0012 | 0.0028 | 0.1089 | 0.0185 | 0.0087 | 0.0001 | 0.0001 | 0.0643 | 0.013 | 0.0007 |
None | 0.0414 | 0.0132 | 0.0168 | 0.0256 | 0.0369 | 0.0061 | 0.0344 | 0.0065 | 0.0088 | 0.0036 | 0.0017 | 0.0021 | 0.0134 | 0.0056 | 0.0043 | 0.0023 | 0.0038 | 0.0046 |
Nuclear Periphery | 0.0046 | 0.0004 | 0.0006 | 0.0005 | 0.0004 | 0.0023 | 0.0054 | 0.0006 | 0.0026 | 0.0034 | 0.002 | 0.0005 | 0.0162 | 0.0003 | 0.0004 | 0.0008 | 0.0007 | 0.0135 |
Nucleolus | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0056 | 0.0014 | 0.0016 | 0.0018 | 0.0013 | 0.0021 | 0.0034 | 0.0009 | 0.0021 | 0.0016 | 0.0014 | 0.0017 | 0.0023 | 0.0006 | 0.0009 | 0.001 | 0.0016 | 0.0016 |
Peroxisomes | 0.0105 | 0.0001 | 0.0005 | 0.0194 | 0.0001 | 0.0001 | 0.0102 | 0 | 0.0022 | 0.0192 | 0.0087 | 0.0009 | 0.0126 | 0.0005 | 0 | 0.002 | 0.0016 | 0.0005 |
Punctate Nuclear | 0.0068 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0082 | 0.0001 | 0.0013 | 0.0037 | 0.0076 | 0.0001 | 0.0014 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0004 |
Vacuole | 0.0013 | 0.0005 | 0.0009 | 0.0013 | 0.0007 | 0.0008 | 0.0033 | 0.0003 | 0.0047 | 0.0009 | 0.007 | 0.0005 | 0.0025 | 0.0003 | 0.0003 | 0.0014 | 0.0011 | 0.0006 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0015 | 0.0001 | 0.001 | 0.0004 | 0.0026 | 0.0002 | 0.002 | 0 | 0.0001 | 0.0008 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.0881 | 11.6387 | 9.7159 | 16.5219 | 24.7931 | 20.3317 | 17.6518 | 12.1619 | 16.4541 | 19.4244 |
Translational Efficiency | 1.651 | 1.8363 | 1.5811 | 1.1697 | 0.9276 | 1.3257 | 1.0722 | 1.2857 | 1.2034 | 1.3356 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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