Standard name
Human Ortholog
Description TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.92 0.95 0.92 0.83 – 0.72 0.59 0.55 0.53 0.61 0.98 0.99 0.99 0.96 0.98 0.96 0.83 0.81 0.61 0.82 0.68 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Mitochondria 0.1 0 0.12 0.16 0.07 0.32 – 0.46 0.63 0.69 0.56 0.59 0 0 0 0 0 0.05 0 0.05 0.19 0.09 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 5 6 2 0 1 1
Bud 1 0 1 0 1 0 – 4 1 1 2 0 0 0 0 0 1 0 5 6 0 0 3 1
Bud Neck 5 1 1 0 0 1 – 2 1 1 0 1 0 0 0 1 1 0 2 1 2 0 0 0
Bud Site 0 0 0 1 0 0 – 0 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 0 0 1 0 2 – 1 0 0 1 0 0 0 2 11 3 1 3 1 2 0 0 0
Cytoplasm 534 267 148 98 146 120 – 119 87 84 55 82 61 133 155 313 195 229 444 224 97 66 50 112
Endoplasmic Reticulum 1 0 0 0 0 1 – 0 0 1 1 0 1 0 0 17 14 14 0 1 3 0 0 0
Endosome 2 3 0 0 2 2 – 0 0 0 0 0 0 0 0 1 0 3 9 5 3 1 4 1
Golgi 0 1 0 0 0 0 – 0 0 0 0 0 0 1 1 1 1 1 2 2 0 0 3 0
Mitochondria 57 6 20 16 11 46 – 76 92 107 58 80 0 0 0 14 2 11 22 12 31 7 3 5
Nucleus 1 0 1 0 1 0 – 1 0 2 0 1 0 0 0 1 2 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 1 0 1 0 0 0
Nucleolus 2 0 1 0 0 0 – 0 0 0 2 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 2 0 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
SpindlePole 3 0 0 1 5 0 – 6 4 4 4 4 0 0 0 0 0 1 10 4 3 0 1 1
Vac/Vac Membrane 3 3 0 0 1 0 – 1 0 2 3 3 0 1 1 8 0 1 9 3 5 1 1 2
Unique Cell Count 562 274 161 103 159 144 166 147 154 104 135 62 135 157 325 198 239 538 278 161 81 75 129
Labelled Cell Count 627 281 172 117 167 173 210 185 203 128 173 62 136 159 368 220 264 538 278 161 81 75 129


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 3.1 3.4 3.9 2.9 – 2.9 2.7 2.8 3.2 2.8 4.4 4.6 4.6 5.0 5.4 5.3 3.9 5.5 4.6
Std Deviation (1e-4) 1.3 1.0 1.1 2.2 2.2 1.4 – 1.1 1.5 1.3 2.2 1.8 1.1 1.5 1.8 1.3 1.7 2.4 1.3 2.4 1.0
Intensity Change (Log2) – – – 0.12 0.34 -0.09 – -0.08 -0.2 -0.16 0.06 -0.12 0.5 0.58 0.56 0.7 0.82 0.78 0.34 0.82 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.048 0.0691 -0.1794 -0.2871 -0.0533 -0.2425 -0.7173 -1.0723 -0.6033 -1.1247 -0.9315 -0.951 0.4052 0.5076 0.2307 0.1058 1.3024 0.6251
Actin 0.0327 0.0021 0.0297 0.0121 0.0166 0.0099 0.0236 0.0007 0.0111 0.0011 0.0145 0.0046 0.0126 0.0019 0.0024 0.0037 0.0284 0.0027
Bud 0.0018 0.0032 0.0003 0.0005 0.0007 0.0002 0.0044 0.0088 0.0132 0.0215 0.0003 0.0056 0.0008 0.0005 0.0008 0.0324 0.0059 0.0001
Bud Neck 0.0041 0.0002 0.0009 0.0003 0.0014 0.0003 0.0024 0.0006 0.0008 0.0023 0.0002 0.0032 0.0009 0 0.0007 0.0008 0.0011 0.0002
Bud Periphery 0.0046 0.0094 0.0004 0.0021 0.001 0.0005 0.0112 0.0086 0.0069 0.0124 0.0004 0.0099 0.0013 0.0006 0.0012 0.0081 0.0077 0.0001
Bud Site 0.0085 0.0081 0.002 0.0011 0.0081 0.0002 0.0122 0.0221 0.0167 0.0181 0.0005 0.002 0.0036 0.0006 0.0119 0.0197 0.0041 0
Cell Periphery 0.0003 0.0006 0.0003 0.0002 0.0002 0.0001 0.0007 0.0004 0.0004 0.0006 0 0.0002 0.0001 0.0001 0.0002 0.0005 0.0003 0
Cytoplasm 0.0312 0.0288 0.0249 0.0349 0.0487 0.0683 0.0353 0.0375 0.0345 0.0337 0.0287 0.0481 0.0325 0.031 0.027 0.0381 0.0422 0.0699
Cytoplasmic Foci 0.0177 0.0053 0.0095 0.0163 0.0188 0.0121 0.0368 0.0192 0.0217 0.0266 0.0173 0.0189 0.0446 0.0013 0.0022 0.0057 0.0235 0.0028
Eisosomes 0.0004 0.0004 0.0003 0.0002 0.0002 0.0004 0.0005 0.0004 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0
Endoplasmic Reticulum 0.0046 0.0165 0.0116 0.0009 0.0007 0.013 0.002 0.0007 0.001 0.001 0.0012 0.0007 0.0016 0.0002 0.001 0.0006 0.004 0.0026
Endosome 0.0109 0.0008 0.0052 0.0067 0.0159 0.0047 0.0088 0.0027 0.0055 0.0007 0.0132 0.002 0.0246 0.0001 0.0045 0.0039 0.0458 0.0074
Golgi 0.0033 0.0004 0.0032 0.0044 0.0148 0.0023 0.0039 0.0002 0.0021 0.0003 0.0043 0.0009 0.0085 0 0.0019 0.0022 0.0249 0.0016
Lipid Particles 0.0101 0.0045 0.0059 0.0233 0.0267 0.0041 0.0112 0.001 0.0048 0.001 0.006 0.0041 0.0068 0 0.0059 0.0017 0.0103 0.0015
Mitochondria 0.0056 0.0012 0.0021 0.0114 0.0165 0.0037 0.0212 0.003 0.0036 0.0025 0.0015 0.0077 0.0035 0.0001 0.0033 0.0131 0.0075 0.0005
None 0.8249 0.9158 0.8893 0.8494 0.7686 0.8653 0.7861 0.8686 0.8271 0.8378 0.8786 0.8683 0.8348 0.9627 0.9264 0.8625 0.6984 0.8931
Nuclear Periphery 0.0151 0.0002 0.0025 0.0009 0.0066 0.0046 0.0086 0.0008 0.0054 0.0012 0.0105 0.0019 0.0018 0.0001 0.0028 0.0003 0.0262 0.0078
Nucleolus 0.0012 0.0003 0.0006 0.0034 0.0012 0.0002 0.0025 0.0022 0.0022 0.0033 0.0009 0.0016 0.0004 0 0.0003 0.0006 0.0017 0.0001
Nucleus 0.0031 0.0003 0.0019 0.001 0.0241 0.0007 0.0043 0.0086 0.0055 0.0054 0.0015 0.0034 0.0012 0.0002 0.0035 0.0014 0.005 0.0068
Peroxisomes 0.0091 0.0005 0.005 0.026 0.0246 0.0039 0.0094 0.0006 0.0198 0.0009 0.0031 0.0043 0.0114 0.0001 0.001 0.002 0.0268 0.0002
Punctate Nuclear 0.008 0.0003 0.0022 0.002 0.0019 0.0046 0.0111 0.0121 0.0157 0.0278 0.016 0.0113 0.0062 0.0001 0.0014 0.0004 0.0318 0.0015
Vacuole 0.0019 0.001 0.0019 0.0022 0.002 0.0005 0.0027 0.0008 0.001 0.0014 0.0003 0.0007 0.002 0.0002 0.001 0.0017 0.0018 0.0005
Vacuole Periphery 0.0011 0.0002 0.0004 0.0007 0.0008 0.0005 0.001 0.0001 0.0006 0.0003 0.0006 0.0006 0.0007 0 0.0004 0.0004 0.0022 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4732 5.8713 10.1657 13.5715 14.1926 4.3762 11.3782 10.6845 23.7033 16.2951
Translational Efficiency 5.8459 5.6022 2.2636 2.0941 1.9388 6.8467 3.1487 2.7032 1.0731 2.0408

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Taf13

Taf13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available