Standard name
Human Ortholog
Description Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.1 0.1 0.08 0.1 0.14 0.07 0.06 0.09 0.07 0.14 0.17 0.18 0.07 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.11 0.06 0.24 0.23 0.19 0.24 0.25 0.33 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.92 0.87 0.84 0.85 0.75 0.72 0.77 0.73 0.79 0.73 0.72 0.81 0.76 0.76 0.9 0.9 0.87 0.81 0.89 0.91 0.88 0.61 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0.05 0.05 0.09 0.07 0.15 0.17 0.15 0.2 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0.08 0.27 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 0 1 3 1 2 4 5 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 4 10 11 8 16 28 7 7 10 11 17 41 42 23 15 17 1 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 4 0 2 2 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 0 2 1 3 1 0 0 1 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 2
Mitochondria 3 6 7 11 7 23 37 39 23 31 37 49 1 1 1 6 2 1 0 0 0 0 2 3
Nucleus 87 130 68 81 95 72 116 157 70 96 83 118 99 184 179 302 268 264 78 131 85 96 121 99
Nuclear Periphery 1 0 0 1 0 2 2 1 1 3 3 3 0 2 0 0 0 0 1 0 0 0 0 4
Nucleolus 0 3 3 6 6 5 15 14 14 21 17 32 5 6 8 5 3 4 1 2 0 2 10 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 6 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4 3 2 0 0 0 0 0 0 0 3 3
Vac/Vac Membrane 6 3 2 1 2 4 3 4 2 2 1 3 0 7 9 9 12 16 4 6 4 8 53 63
Unique Cell Count 96 141 78 96 112 96 161 204 96 122 113 164 122 242 236 336 298 303 97 147 94 110 199 207
Labelled Cell Count 100 146 84 112 122 114 190 246 119 163 155 222 130 253 250 349 303 308 97 147 94 110 199 207


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.5 7.0 6.2 7.0 7.0 5.8 6.2 6.5 5.6 6.1 5.7 7.8 8.5 9.1 10.1 11.9 12.2 7.2 7.6 8.7
Std Deviation (1e-4) 2.2 1.5 2.2 1.5 2.0 3.0 2.1 2.4 2.6 1.6 2.5 2.5 2.4 3.3 3.8 2.4 2.5 2.8 1.9 1.9 2.0
Intensity Change (Log2) – – – -0.17 -0.0 0.01 -0.27 -0.17 -0.11 -0.32 -0.19 -0.28 0.17 0.29 0.38 0.54 0.77 0.81 0.05 0.12 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.2 0.8 1.3 2.0 0 0 1.0 0 2.0 2.6 2.7 0.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 -0.7 2.6 2.6 2.1 2.6 2.9 3.8 3.6 0 0 0 0 0 0
Nucleus -0.5 -0.5 -2.0 -2.6 -1.9 -2.3 -1.5 -2.3 -2.6 -1.1 -2.1 -2.1 0.7 0.7 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.5 0 2.4 2.8 2.5 3.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0291 4.3639 3.6149 3.6967 2.4272 3.3798 4.2783 4.6129 4.8327 4.8198 3.9127 4.3905 6.1645 6.2195 6.181 5.5867 5.5644 5.7521
Actin 0.0213 0 0.0115 0 0.008 0.0071 0.0027 0 0.0085 0.0135 0.0084 0.0017 0.0075 0 0.0031 0.0001 0.0053 0
Bud 0.0003 0.0011 0.0001 0 0 0.0015 0.0002 0 0.0003 0.001 0.0004 0.0001 0.0004 0 0.0001 0 0.0002 0
Bud Neck 0.0032 0.0001 0.0053 0 0.0003 0.0008 0.001 0 0.001 0.0048 0.0004 0.0012 0.0006 0 0.0002 0.0009 0.0002 0.0003
Bud Periphery 0.0004 0.0003 0.0001 0 0.0001 0.0011 0.0008 0 0.0007 0.0019 0.0008 0.0003 0.0014 0 0.0002 0 0.0002 0
Bud Site 0.0045 0.0002 0.0009 0 0.0003 0.0002 0.0008 0 0.0046 0.0123 0.0044 0.0001 0.0006 0 0.0003 0.0005 0.0002 0
Cell Periphery 0.0002 0 0.0001 0 0 0.0007 0.0004 0 0.0005 0.0007 0.0001 0.0001 0.0002 0 0.0001 0.0001 0 0
Cytoplasm 0.0084 0.007 0.0001 0 0.0006 0.0078 0.0021 0.0001 0.0007 0.0402 0.0003 0.0003 0.0006 0.0001 0.0003 0.0022 0.0002 0.0001
Cytoplasmic Foci 0.0252 0.0001 0.0011 0 0.014 0.0033 0.0083 0.0001 0.001 0.0129 0.0109 0.0004 0.0107 0 0.0013 0.0039 0.0013 0
Eisosomes 0.0002 0 0.0001 0 0.0034 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0017 0 0.0001 0 0.0001 0.0007 0.0028 0.0001 0.0004 0.0061 0.002 0.0011 0.0006 0 0.0009 0.0003 0.0002 0
Endosome 0.0105 0.0001 0.0013 0 0.0041 0.0038 0.0093 0.0018 0.0037 0.0121 0.0126 0.0025 0.0021 0 0.0038 0.0109 0.0066 0
Golgi 0.0042 0 0.0019 0 0.0031 0.0011 0.0019 0 0.0022 0.0055 0.0216 0.0005 0.0007 0 0.0011 0.0016 0.0116 0
Lipid Particles 0.0042 0 0.0019 0 0.0538 0.0077 0.0092 0.0003 0.0024 0.0055 0.0113 0.0019 0.0015 0 0.0031 0.0042 0.0009 0
Mitochondria 0.0028 0.0004 0.0006 0.0001 0.0015 0.0083 0.0126 0.0002 0.0056 0.0099 0.0327 0.0004 0.0055 0.0002 0.0095 0.0005 0.025 0.0001
None 0.0023 0.0009 0.0001 0 0.0493 0.0018 0.0011 0.0001 0.0003 0.0008 0.0004 0.0002 0.0003 0.0001 0.0004 0.0191 0.0001 0
Nuclear Periphery 0.0064 0.0016 0.0018 0.0006 0.001 0.0021 0.0111 0.0091 0.0053 0.0266 0.0059 0.0024 0.0211 0.0041 0.0098 0.0013 0.0011 0.0012
Nucleolus 0.0132 0.0108 0.0116 0.0053 0.0305 0.0294 0.0155 0.0116 0.0058 0.0077 0.0072 0.0168 0.0189 0.0057 0.0081 0.0045 0.018 0.0214
Nucleus 0.8729 0.9736 0.9549 0.9937 0.7827 0.9152 0.8883 0.9703 0.9533 0.8279 0.8514 0.9667 0.9051 0.9879 0.9469 0.9391 0.9273 0.9757
Peroxisomes 0.002 0 0.0012 0 0.0417 0.0029 0.0037 0 0.0015 0.0031 0.0226 0.0023 0.0009 0 0.0011 0.0006 0.0004 0
Punctate Nuclear 0.014 0.002 0.0048 0.0002 0.0051 0.0035 0.0241 0.0004 0.0009 0.0026 0.0023 0.0009 0.018 0.0016 0.0083 0.0023 0.0006 0.0012
Vacuole 0.0014 0.0011 0.0002 0 0.0002 0.0006 0.0027 0.0031 0.0008 0.0039 0.0017 0.0002 0.0017 0.0001 0.0006 0.007 0.0003 0.0001
Vacuole Periphery 0.0007 0.0005 0.0001 0 0.0001 0.0003 0.0014 0.0026 0.0004 0.001 0.0023 0.0001 0.0016 0.0002 0.0006 0.0007 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.8794 12.6189 14.0456 17.2264 19.3189 21.1624 12.7589 10.0467 16.3571 21.2219
Translational Efficiency 0.9678 0.8179 0.8665 1.3634 1.1325 1.2071 1.0264 1.0953 0.9456 0.8308

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Cac2

Cac2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cac2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available