Standard name | |
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Human Ortholog | |
Description | Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 4 | 4 | 13 | 3 | 19 | 16 | 13 | 13 | 14 | 35 | 0 | 1 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 446 | 245 | 83 | 247 | 375 | 165 | 382 | 488 | 252 | 332 | 299 | 400 | 270 | 382 | 528 | 188 | 149 | 140 | 464 | 227 | 85 | 181 | 245 | 382 |
Endoplasmic Reticulum | 6 | 0 | 2 | 4 | 13 | 4 | 31 | 32 | 16 | 30 | 21 | 33 | 2 | 1 | 3 | 4 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 2 | 4 | 0 | 3 | 0 | 0 | 0 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 4 | 1 | 2 | 2 | 3 | 12 | 5 | 9 | 6 | 7 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 5 | 0 | 0 | 0 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 11 | 5 | 8 | 10 | 13 | 4 | 22 | 5 | 16 | 0 | 1 | 0 | 3 | 3 | 4 | 0 | 5 | 0 | 0 | 1 | 3 |
Unique Cell Count | 452 | 245 | 88 | 261 | 392 | 179 | 426 | 546 | 283 | 395 | 337 | 465 | 271 | 385 | 529 | 195 | 156 | 144 | 475 | 243 | 90 | 188 | 256 | 396 |
Labelled Cell Count | 459 | 250 | 93 | 268 | 408 | 186 | 445 | 566 | 296 | 416 | 347 | 497 | 272 | 386 | 534 | 202 | 160 | 152 | 475 | 243 | 90 | 188 | 256 | 396 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 29.4 | 36.0 | 28.0 | 30.3 | 28.5 | 17.0 | 23.0 | 21.9 | 21.1 | 21.0 | 22.0 | 22.7 | 34.8 | 31.7 | 31.3 | 29.6 | 31.0 | 32.2 | 31.2 | 33.7 | 32.4 |
Std Deviation (1e-4) | 6.5 | 7.6 | 5.9 | 6.0 | 5.8 | 6.0 | 4.6 | 4.5 | 4.6 | 4.7 | 4.4 | 4.6 | 7.0 | 7.5 | 6.5 | 10.4 | 10.7 | 12.0 | 7.2 | 7.5 | 7.5 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.02 | -0.72 | -0.29 | -0.36 | -0.41 | -0.42 | -0.35 | -0.31 | 0.31 | 0.18 | 0.16 | 0.08 | 0.15 | 0.2 | 0.16 | 0.26 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 0.5 | -0.6 | -1.4 | -1.4 | -1.5 | -2.5 | -1.5 | -2.1 | 3.4 | 3.2 | 4.9 | 0.8 | 0.4 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1.7 | 0 | 0 | 1.8 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.0603 | 30.3203 | 26.6957 | 25.5633 | 23.7794 | 29.0682 | 33.2857 | 42.4168 | 39.1389 | 37.6405 | 36.1803 | 40.4134 | 31.9066 | 41.176 | 35.4335 | 34.9896 | 31.8516 | 36.1487 |
Actin | 0.0161 | 0.0006 | 0.0105 | 0.002 | 0.0363 | 0.0097 | 0.0457 | 0.0002 | 0.0013 | 0.0131 | 0.0003 | 0.0003 | 0.0171 | 0.0002 | 0.0116 | 0.0001 | 0.0181 | 0.0013 |
Bud | 0.0008 | 0.0003 | 0.0003 | 0.0016 | 0.0003 | 0.0001 | 0.0016 | 0 | 0.0009 | 0.0028 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 |
Bud Neck | 0.0011 | 0.0005 | 0.0004 | 0.0006 | 0.0007 | 0.001 | 0.0009 | 0.0001 | 0.0002 | 0.0012 | 0.0004 | 0.0005 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0013 |
Bud Periphery | 0.0009 | 0.0001 | 0.0003 | 0.0014 | 0.0004 | 0.0001 | 0.0011 | 0 | 0.0004 | 0.0049 | 0.0016 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 |
Bud Site | 0.0031 | 0.0002 | 0.0032 | 0.004 | 0.0008 | 0.0001 | 0.0075 | 0 | 0.0004 | 0.004 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0008 | 0 | 0.0013 | 0.0003 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0008 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8611 | 0.9869 | 0.909 | 0.8735 | 0.8747 | 0.9514 | 0.8866 | 0.9958 | 0.966 | 0.8826 | 0.8866 | 0.9679 | 0.9111 | 0.9964 | 0.9366 | 0.9856 | 0.86 | 0.9588 |
Cytoplasmic Foci | 0.0138 | 0.0005 | 0.0128 | 0.0064 | 0.004 | 0.0037 | 0.0018 | 0.0001 | 0.0044 | 0.0012 | 0.0003 | 0.0023 | 0.008 | 0.0001 | 0.0087 | 0.0004 | 0.0032 | 0.0018 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0005 | 0.0017 | 0.0016 | 0.0009 | 0.001 | 0.0087 | 0.0001 | 0.0025 | 0.0091 | 0.0006 | 0.0007 | 0.0058 | 0.0001 | 0.0034 | 0.0001 | 0.001 | 0.0003 |
Endosome | 0.0138 | 0.0002 | 0.009 | 0.0202 | 0.0061 | 0.0035 | 0.0074 | 0 | 0.0028 | 0.0343 | 0.0046 | 0.0025 | 0.0046 | 0 | 0.002 | 0.0001 | 0.0112 | 0.0024 |
Golgi | 0.0062 | 0 | 0.0035 | 0.002 | 0.0046 | 0.0017 | 0.0027 | 0 | 0.0005 | 0.0041 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0016 | 0 | 0.009 | 0.0031 |
Lipid Particles | 0.0081 | 0 | 0.007 | 0.0087 | 0.0081 | 0.0002 | 0.0004 | 0 | 0.0014 | 0.0005 | 0.0001 | 0.0002 | 0.0026 | 0 | 0.0029 | 0 | 0.017 | 0.0057 |
Mitochondria | 0.0092 | 0.0001 | 0.0042 | 0.025 | 0.0218 | 0.0009 | 0.0029 | 0 | 0.0012 | 0.004 | 0.0011 | 0.0003 | 0.0005 | 0 | 0.0087 | 0 | 0.0631 | 0.0027 |
None | 0.0034 | 0.0003 | 0.0057 | 0.0038 | 0.0018 | 0.0002 | 0.0011 | 0.0001 | 0.0017 | 0.0007 | 0.0002 | 0.0008 | 0.0046 | 0.0001 | 0.0113 | 0.0002 | 0.0008 | 0.0002 |
Nuclear Periphery | 0.0097 | 0.0014 | 0.0053 | 0.0035 | 0.0034 | 0.0036 | 0.0102 | 0.0003 | 0.0021 | 0.0113 | 0.0047 | 0.0034 | 0.0072 | 0.0002 | 0.0017 | 0.0008 | 0.0018 | 0.0018 |
Nucleolus | 0.0012 | 0 | 0.0007 | 0.0021 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 |
Nucleus | 0.0245 | 0.0068 | 0.0151 | 0.0189 | 0.0208 | 0.0216 | 0.0173 | 0.0032 | 0.0098 | 0.0099 | 0.0187 | 0.0159 | 0.0168 | 0.0026 | 0.0071 | 0.0122 | 0.0057 | 0.0174 |
Peroxisomes | 0.0059 | 0 | 0.0058 | 0.0017 | 0.0108 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0016 | 0 | 0.0033 | 0.0014 |
Punctate Nuclear | 0.0095 | 0.0001 | 0.0026 | 0.0013 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0107 | 0 | 0.0003 | 0.0002 | 0.0008 | 0.0003 |
Vacuole | 0.0047 | 0.0013 | 0.0019 | 0.0147 | 0.0019 | 0.0005 | 0.0013 | 0 | 0.003 | 0.0088 | 0.0525 | 0.0027 | 0.0074 | 0 | 0.0004 | 0 | 0.0006 | 0.0004 |
Vacuole Periphery | 0.003 | 0.0001 | 0.0007 | 0.0061 | 0.0014 | 0.0002 | 0.0011 | 0 | 0.0006 | 0.0059 | 0.0259 | 0.0008 | 0.0011 | 0 | 0.0003 | 0 | 0.0011 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 302.9906 | 330.2405 | 309.5402 | 346.959 | 266.7955 | 179.7505 | 305.3481 | 324.702 | 294.0953 | 250.5861 |
Translational Efficiency | 1.0873 | 1.2805 | 1.3041 | 1.1031 | 1.15 | 1.6132 | 1.2474 | 1.0394 | 1.101 | 1.0587 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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