Standard name
Human Ortholog
Description Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.2 0.38 0.45 0.25 0.26 0.18 0.15 0.1 0.09 0.1 0.2 0.16 0.12 0.05 0.06 0.06 0 0.05 0 0 0 0
Bud 0 0 0.06 0 0 0.07 0.1 0.12 0.12 0.1 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0.88 0.87 0.87 0.72 0.55 0.31 0.19 0.14 0.19 0.14 0.87 0.88 0.85 0.85 0.85 0.8 0.81 0.76 0.88 0.82 0.83 0.76
Cytoplasm 0.18 0.13 0.12 0.13 0.17 0.35 0.4 0.49 0.66 0.68 0.66 0.12 0.08 0.1 0.23 0.23 0.24 0.05 0.06 0 0 0 0
Endoplasmic Reticulum 0 0.08 0 0 0.16 0.1 0.12 0.05 0 0 0 0.05 0 0.05 0 0 0 0.07 0.09 0 0.07 0.09 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.15 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.14 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 12 96 108 85 66 78 76 42 26 38 72 77 45 10 20 8 3 4 4 3 7 14
Bud 4 0 15 8 8 18 42 63 51 28 41 12 8 6 10 5 0 2 0 2 3 2 5
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 1 3 3 1 6
Bud Site 0 0 0 0 0 1 4 14 7 3 4 0 0 1 0 0 0
Cell Periphery 139 53 217 208 243 139 132 98 62 53 53 318 423 330 159 307 107 135 79 244 236 378 370
Cytoplasm 28 8 29 31 57 88 169 253 289 191 248 45 37 38 42 82 32 8 6 3 3 6 9
Endoplasmic Reticulum 3 5 3 5 53 25 49 25 5 6 7 17 19 18 7 11 3 11 9 10 20 39 53
Endosome 0 0 1 0 0 2 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 1 0 0 0 0 4 22 11 1 1 0 0 0 0 0 0 0
Mitochondria 1 0 2 1 1 5 8 23 52 42 52 0 0 2 6 3 4 1 0 5 2 5 4
Nucleus 1 1 0 0 1 1 8 9 5 1 4 0 1 2 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 2 21 10 6 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 3 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 3 1 1 1 4 1 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 0 2 4 19 34 9 6 12 0 3 2 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 3 9 36 72 33 10 26 1 3 2 0 0 2 1 1 1 9 8 16
Unique Cell Count 155 60 250 239 339 252 425 521 441 279 378 367 482 386 186 362 133 168 105 279 288 454 486
Labelled Cell Count 198 79 366 361 453 359 553 692 567 373 494 473 593 457 235 431 156 168 105 279 288 454 486


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 30.0 19.8 21.3 17.6 11.4 9.9 9.0 6.1 6.0 5.6 30.7 30.5 28.4 28.4 34.4 40.7 23.7 21.3 20.1
Std Deviation (1e-4) 7.1 8.7 6.2 6.5 5.6 4.9 4.8 4.3 3.2 3.0 2.9 7.9 8.2 8.5 7.0 9.6 13.0 7.4 5.3 5.8
Intensity Change (Log2) 0.11 -0.17 -0.79 -1.0 -1.14 -1.7 -1.72 -1.83 0.64 0.62 0.52 0.52 0.8 1.04 0.26 0.11 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 -3.5 -2.9 -5.8 -7.4 -9.1 -7.9 -8.5 -5.1 -6.8 -7.9 -8.0 -10.2 -6.8
Bud -1.4 -2.3 0.5 1.8 2.6 2.4 1.7 2.1 -1.6 -3.2 -3.1 -0.3 -3.2 -2.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -4.4 -7.8 -14.0 -18.0 -18.7 -15.6 -18.0 -0.1 0.4 -0.5 -0.4 -0.7 -1.6
Cytoplasm 0.5 1.8 6.2 7.8 10.0 13.7 13.2 13.3 0.2 -1.8 -0.7 3.1 3.5 3.2
Endoplasmic Reticulum 0 5.9 4.3 4.9 2.5 0 0 0 2.4 2.1 2.4 0 1.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.1 0 0 0 0
Mitochondria 0 0 0 0 2.7 5.2 5.9 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2.8 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3.0 3.8 0 0 2.4 0 0 0 0 0 0
Vacuole 0 0 0 4.7 6.2 4.4 0 4.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.524 45.1951 38.9789 40.0712 32.1268 42.3026 33.3188 51.9674 43.5783 41.3195 42.6743 48.6523 25.2411 48.9716 46.0002 40.1442 43.4317 42.5765
Actin 0.0236 0 0.0004 0.0002 0.0011 0.0003 0.0092 0 0.0053 0.004 0.0012 0.0001 0.0203 0 0.0149 0.0023 0.0001 0
Bud 0.0042 0.0001 0.0004 0.0003 0.0049 0.0002 0.0013 0.0001 0.0011 0.0022 0.0005 0.0002 0.0008 0.0002 0.0003 0.0003 0.0009 0.0002
Bud Neck 0.0021 0.0002 0.0009 0.0005 0.0027 0.0013 0.0014 0.0001 0.0004 0.0009 0.0147 0.0011 0.0011 0.0005 0.0006 0.003 0.0021 0.0008
Bud Periphery 0.0278 0.0006 0.0044 0.0053 0.0314 0.0026 0.0038 0.0006 0.0055 0.0107 0.0041 0.0053 0.0014 0.0013 0.0027 0.0046 0.0152 0.0063
Bud Site 0.0062 0.0018 0.0029 0.0005 0.0043 0.0009 0.0041 0.0044 0.004 0.006 0.0025 0.0001 0.008 0.0046 0.0025 0.0005 0.0012 0.0002
Cell Periphery 0.6272 0.786 0.7628 0.6638 0.627 0.7664 0.7518 0.8598 0.7989 0.7704 0.7512 0.8092 0.5545 0.8712 0.8248 0.6586 0.846 0.8498
Cytoplasm 0.0224 0.0386 0.0229 0.0097 0.0317 0.0123 0.0101 0.0058 0.0063 0.0018 0.0032 0.0027 0.0152 0.0104 0.0101 0.0022 0.0035 0.0037
Cytoplasmic Foci 0.0016 0 0.0027 0 0.0002 0.0013 0.0132 0 0.0018 0.0002 0.0029 0 0.1067 0 0.0057 0 0 0
Eisosomes 0.0056 0.0002 0.0009 0.0003 0.0002 0.0004 0.001 0.0002 0.0011 0.0007 0.0014 0.0004 0.0023 0.0002 0.0002 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0708 0.053 0.0546 0.1555 0.082 0.0499 0.0432 0.0284 0.0405 0.0459 0.054 0.062 0.0271 0.015 0.0228 0.0867 0.0123 0.0397
Endosome 0.0091 0 0.0048 0.0002 0.0014 0.0002 0.0097 0 0.0036 0.0007 0.011 0 0.0202 0 0.0091 0.0001 0 0
Golgi 0.0023 0 0.0011 0.0001 0.0002 0.0001 0.0027 0 0.0058 0.0006 0.0076 0 0.0109 0 0.0059 0.0001 0 0
Lipid Particles 0.0062 0.0063 0.0053 0.0032 0.0075 0.0037 0.0083 0.0009 0.0038 0.0019 0.0074 0.0013 0.0181 0.0008 0.0054 0.0011 0.0014 0.0008
Mitochondria 0.0163 0 0.0019 0.0001 0.0038 0.0005 0.0057 0 0.0133 0.0089 0.0054 0 0.0044 0.0001 0.0003 0.0016 0 0
None 0.0072 0 0.0085 0.0001 0.0006 0.0033 0.0082 0 0.0002 0.0001 0.0001 0 0.0236 0 0.0004 0.0002 0 0
Nuclear Periphery 0.0225 0.0133 0.0147 0.0146 0.0117 0.007 0.0262 0.014 0.0176 0.0135 0.0113 0.0154 0.0271 0.011 0.0123 0.1294 0.0079 0.0071
Nucleolus 0.0046 0.0001 0.001 0.0001 0.0045 0.0017 0.0013 0.0001 0.0003 0.0006 0.0011 0.0001 0.0022 0.0002 0.0002 0.0006 0.0003 0.0001
Nucleus 0.0049 0.0002 0.0008 0.0004 0.0105 0.0014 0.0024 0.0003 0.0005 0.0008 0.001 0.0003 0.003 0.0005 0.0006 0.0041 0.0006 0.0003
Peroxisomes 0.0016 0 0.0004 0 0.0001 0.0001 0.0048 0 0.0017 0.0001 0.0015 0 0.0619 0 0.0002 0 0 0
Punctate Nuclear 0.0018 0 0.0009 0 0.0001 0.0011 0.0052 0 0.0001 0 0.0002 0 0.025 0 0.0002 0.0002 0 0
Vacuole 0.1283 0.0991 0.1066 0.1444 0.168 0.1447 0.0849 0.085 0.0873 0.1274 0.1162 0.1008 0.0645 0.0835 0.08 0.097 0.106 0.0901
Vacuole Periphery 0.0038 0.0002 0.001 0.0008 0.0058 0.0005 0.0015 0.0003 0.0008 0.0026 0.0015 0.001 0.0015 0.0005 0.0009 0.0071 0.0021 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1603 12.1102 11.6822 15.5482 12.295 20.9863 20.3567 21.2805 22.9715 18.0777
Translational Efficiency 1.0165 0.8941 0.9667 0.5248 0.4454 0.8829 0.868 0.6679 0.7248 0.5294

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cell periphery (64%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Atr1

Atr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available