Standard name | |
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Human Ortholog | |
Description | 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 1 | 6 | 6 | 3 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 0 | 10 | 6 | 9 | 13 | 16 | 22 | 13 | 6 | 10 | 7 | 8 | 11 | 18 | 18 | 35 | 18 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 7 | 7 | 28 | 20 | 37 | 47 | 74 | 38 | 53 | 3 | 11 | 10 | 26 | 20 | 17 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 239 | 117 | 155 | 143 | 138 | 176 | 267 | 300 | 216 | 229 | 146 | 179 | 87 | 121 | 182 | 160 | 94 | 117 | 235 | 119 | 148 | 69 | 109 | 84 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 25 | 13 | 20 | 24 | 35 | 66 | 96 | 127 | 133 | 150 | 87 | 136 | 21 | 35 | 62 | 29 | 9 | 12 | 4 | 0 | 7 | 7 | 11 | 22 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 1 | 2 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 5 | 5 | 6 | 3 | 1 | 6 | 5 | 32 | 46 |
Unique Cell Count | 246 | 120 | 172 | 166 | 169 | 228 | 335 | 388 | 301 | 323 | 214 | 266 | 100 | 148 | 227 | 209 | 129 | 149 | 249 | 124 | 171 | 87 | 160 | 163 |
Labelled Cell Count | 267 | 130 | 187 | 183 | 190 | 283 | 414 | 489 | 418 | 467 | 284 | 383 | 121 | 181 | 280 | 242 | 167 | 172 | 249 | 124 | 171 | 87 | 160 | 163 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 40.8 | 49.1 | 39.1 | 37.3 | 36.0 | 32.6 | 31.5 | 34.6 | 32.8 | 29.9 | 32.0 | 30.7 | 53.4 | 56.0 | 55.8 | 76.0 | 86.7 | 96.2 | 37.5 | 47.6 | 52.2 |
Std Deviation (1e-4) | 11.9 | 16.3 | 18.2 | 15.4 | 15.1 | 15.6 | 16.3 | 16.6 | 13.9 | 12.8 | 16.0 | 12.9 | 21.4 | 23.4 | 27.8 | 38.2 | 50.8 | 47.9 | 15.4 | 21.5 | 22.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.12 | -0.27 | -0.31 | -0.18 | -0.26 | -0.39 | -0.29 | -0.35 | 0.45 | 0.52 | 0.51 | 0.96 | 1.15 | 1.3 | -0.06 | 0.28 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.2 | 0 | -0.5 | -0.1 | -0.7 | -2.4 | -0.5 | -1.7 | 0.7 | 0.6 | 0.8 | 1.0 | 5.1 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.5 | 2.9 | 3.9 | 5.2 | 6.6 | 5.6 | 6.0 | 0 | 3.2 | 0 | 4.5 | 5.0 | 4.2 |
Nucleus | -1.1 | -2.2 | -3.4 | -3.0 | -3.6 | -4.7 | -4.9 | -5.2 | -5.5 | -0.8 | -2.2 | -2.7 | -3.5 | -3.9 | -2.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.8 | 2.3 | 4.2 | 4.3 | 5.2 | 7.3 | 7.8 | 6.3 | 8.4 | 2.1 | 2.8 | 3.8 | 0.7 | -1.4 | -1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 36.0821 | 41.1731 | 37.3626 | 39.3216 | 35.021 | 36.2594 | 37.3898 | 37.4083 | 36.2736 | 33.3979 | 25.0846 | 34.291 | 37.1771 | 43.7499 | 41.182 | 40.6073 | 38.5456 | 37.6662 |
Actin | 0.0024 | 0.0018 | 0 | 0 | 0.0005 | 0.0009 | 0.0008 | 0 | 0.0004 | 0 | 0.0312 | 0.0005 | 0.0113 | 0 | 0.0028 | 0 | 0 | 0 |
Bud | 0.0003 | 0.0013 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0019 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0005 | 0.002 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0035 | 0.0001 | 0.0023 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0005 | 0.0007 | 0.0001 | 0 | 0.0007 | 0.0007 | 0.0007 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0005 | 0.003 | 0.0001 | 0.0003 | 0.0004 | 0.0014 | 0.0033 | 0 | 0.0004 | 0 | 0.0012 | 0 | 0.001 | 0.0002 | 0.0006 | 0 | 0 | 0.0002 |
Cytoplasmic Foci | 0.0153 | 0.0096 | 0.0039 | 0.0015 | 0.0132 | 0.0017 | 0.0035 | 0 | 0.0031 | 0 | 0.03 | 0.0004 | 0.006 | 0.003 | 0.0042 | 0 | 0 | 0.0009 |
Eisosomes | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0007 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0008 | 0 | 0 | 0 |
Endosome | 0.0048 | 0.0011 | 0.0001 | 0.0002 | 0.0098 | 0.0026 | 0.0032 | 0 | 0.0033 | 0 | 0.028 | 0.0029 | 0.0076 | 0 | 0.0023 | 0 | 0 | 0 |
Golgi | 0.0037 | 0.0006 | 0 | 0 | 0.0045 | 0.0002 | 0.0004 | 0 | 0.0014 | 0 | 0.0325 | 0.0016 | 0.0021 | 0 | 0.0004 | 0 | 0 | 0 |
Lipid Particles | 0.0078 | 0.001 | 0.0015 | 0.0002 | 0.0158 | 0.0006 | 0.0021 | 0 | 0.0019 | 0 | 0.0169 | 0.0003 | 0.0042 | 0.0004 | 0.0014 | 0 | 0 | 0.0003 |
Mitochondria | 0.0051 | 0.0119 | 0.0022 | 0.0002 | 0.033 | 0.0047 | 0.0031 | 0.0004 | 0.0056 | 0.0002 | 0.0332 | 0.0016 | 0.0045 | 0.0008 | 0.0014 | 0.0002 | 0.0026 | 0.0022 |
None | 0.0026 | 0.0023 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0026 | 0 | 0.0003 | 0 | 0.0016 | 0 | 0.0031 | 0.0006 | 0.001 | 0 | 0 | 0.0002 |
Nuclear Periphery | 0.0012 | 0.0006 | 0.0005 | 0.0018 | 0.0026 | 0.0004 | 0.0033 | 0.0037 | 0.0006 | 0.0001 | 0.0018 | 0.0004 | 0.0029 | 0.0032 | 0.0035 | 0.0001 | 0.0004 | 0.0001 |
Nucleolus | 0.0837 | 0.0472 | 0.033 | 0.0183 | 0.1712 | 0.1109 | 0.0634 | 0.0171 | 0.0228 | 0.0071 | 0.1679 | 0.0721 | 0.0844 | 0.0243 | 0.0175 | 0.0051 | 0.1193 | 0.0676 |
Nucleus | 0.859 | 0.9119 | 0.9567 | 0.9423 | 0.7328 | 0.8707 | 0.9011 | 0.9782 | 0.9572 | 0.9926 | 0.598 | 0.9188 | 0.8528 | 0.9659 | 0.9617 | 0.9947 | 0.8768 | 0.9268 |
Peroxisomes | 0.0057 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.001 | 0 | 0.0429 | 0.0001 | 0.0041 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0035 | 0.0008 | 0.0002 | 0.0348 | 0.0012 | 0.0001 | 0.005 | 0 | 0.0002 | 0 | 0.0053 | 0.0001 | 0.0086 | 0.0009 | 0.0014 | 0 | 0.0001 | 0.0001 |
Vacuole | 0.0011 | 0.0015 | 0.0004 | 0 | 0.0037 | 0.0024 | 0.0027 | 0.0001 | 0.0003 | 0 | 0.001 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0014 | 0.001 | 0.0007 | 0.0001 | 0.0067 | 0.0016 | 0.0032 | 0.0005 | 0.0006 | 0 | 0.0022 | 0.0003 | 0.001 | 0.0004 | 0.0003 | 0 | 0.0005 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 337.5251 | 298.0513 | 291.5833 | 344.2054 | 310.6444 | 238.3409 | 319.7721 | 296.431 | 276.1417 | 356.625 |
Translational Efficiency | 1.6545 | 1.8226 | 1.7709 | 1.4004 | 1.6927 | 2.064 | 1.7274 | 1.5005 | 1.7087 | 1.5437 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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