ORF
Human Ortholog
Description Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.98 0.98 0.97 0.96 0.91 0.89 0.85 0.85 0.72 0.82 1.0 1.0 1.0 0.97 0.94 0.95 0.69 0.85 0.8 0.88 0.89 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.05 0.12 0.14 0.28 0.19 0.44 0.25 0 0 0 0 0 0.05 0.1 0.06 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 3 1 3 0 2 2
Bud 0 0 1 2 0 0 3 6 6 6 8 5 0 0 0 0 0 0 1 2 4 0 2 4
Bud Neck 0 0 7 4 14 6 8 19 8 15 3 11 5 0 0 1 1 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0 0 0 0
Cell Periphery 2 0 5 3 0 5 1 4 5 1 0 1 1 0 1 4 2 1 0 0 1 1 3 2
Cytoplasm 155 169 179 335 407 387 549 568 403 432 219 289 237 202 294 97 91 62 113 150 144 96 246 275
Endoplasmic Reticulum 1 1 2 1 2 2 2 2 1 0 1 0 0 0 0 7 6 4 3 0 0 0 0 1
Endosome 0 0 0 0 3 3 0 4 0 0 0 1 0 0 0 0 0 1 2 1 1 0 0 2
Golgi 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 3 3 0 0 1 1
Mitochondria 25 3 0 3 6 22 72 86 132 95 135 87 0 0 1 2 2 3 16 10 17 3 11 10
Nucleus 0 0 0 2 2 3 2 1 3 4 0 1 0 0 0 1 0 0 0 0 0 0 0 1
Nuclear Periphery 2 1 0 1 0 0 0 2 2 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 3 4 10 12 2 6 3 6 0 0 1 0 0 0 1 0 1 0 2 5
Vac/Vac Membrane 1 0 0 2 6 6 13 11 2 6 4 6 1 0 0 1 5 0 3 4 1 1 0 5
Unique Cell Count 164 172 182 341 420 405 603 637 476 508 306 353 238 202 295 100 97 65 163 178 181 110 277 322
Labelled Cell Count 186 174 194 355 443 438 662 719 567 568 374 408 244 202 297 113 108 71 163 178 181 110 277 322


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.6 4.4 4.6 4.9 4.1 4.1 3.3 3.5 2.9 3.4 5.0 5.0 4.8 5.3 6.4 5.7 4.4 4.6 5.1
Std Deviation (1e-4) 1.2 1.2 1.2 1.7 1.6 2.7 1.9 1.6 1.0 1.3 0.8 1.0 1.5 1.2 1.5 1.7 2.8 2.9 1.8 1.1 1.9
Intensity Change (Log2) -0.08 0.0 0.07 -0.15 -0.19 -0.5 -0.38 -0.65 -0.42 0.11 0.11 0.06 0.2 0.48 0.31 -0.07 -0.01 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0095 0.5284 0.0111 0.0785 -0.0376 0.3441 0.092 0.4649 0.2851 0.6418 0.2529 0.2437 -1.1278 -1.1354 -1.3388 -1.0302 -1.3891 -0.9816
Actin 0.0185 0.0004 0.0004 0.0002 0.0068 0.0066 0.0365 0.0018 0.017 0.0056 0.0179 0.0075 0.0453 0.0011 0.007 0.0005 0.0508 0.0026
Bud 0.0044 0.01 0.004 0 0.0004 0.0006 0.0049 0.0007 0.0045 0.0004 0.0061 0.0005 0.0016 0.0024 0.001 0.0001 0.0093 0.0012
Bud Neck 0.0002 0.0002 0.0039 0.0001 0.0002 0.0061 0.0038 0.0003 0.0025 0.0018 0.0015 0.0025 0.006 0.0001 0.0008 0.0001 0.0052 0.0067
Bud Periphery 0.0021 0.0051 0.0014 0 0.0005 0.0005 0.0059 0.0019 0.005 0.0007 0.004 0.0008 0.0032 0.0024 0.0009 0.0001 0.0124 0.0016
Bud Site 0.0036 0.0071 0.0005 0 0.0003 0.0002 0.0065 0.0023 0.0111 0.0038 0.0065 0.0005 0.0183 0.0089 0.0098 0.0002 0.0166 0.0049
Cell Periphery 0.0002 0.0003 0.0001 0 0 0.0001 0.0004 0.0002 0.0004 0.0002 0.0009 0 0.0005 0.0001 0.0002 0 0.0002 0.0001
Cytoplasm 0.1615 0.2733 0.1419 0.2521 0.2486 0.2548 0.0976 0.1807 0.1275 0.1766 0.1136 0.1815 0.0903 0.2083 0.1408 0.1196 0.0795 0.237
Cytoplasmic Foci 0.0128 0.0046 0.003 0.0019 0.0071 0.0083 0.0169 0.0059 0.0075 0.0442 0.0203 0.0091 0.0313 0.0058 0.0163 0.0098 0.0121 0.0136
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0002 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0018 0.0053 0.0005 0.0006 0.0006 0.0007 0.0024 0.0006 0.0017 0.0008 0.0018 0.0006 0.002 0.0012 0.0019 0.0006 0.0022 0.0009
Endosome 0.0024 0.0113 0.0007 0.0004 0.001 0.0017 0.0124 0.0011 0.0053 0.0377 0.0207 0.0045 0.015 0.0013 0.0097 0.0013 0.0075 0.0065
Golgi 0.0015 0.0003 0.0001 0 0.0016 0.0002 0.0057 0.0001 0.0023 0.0075 0.0029 0.0023 0.0051 0.0003 0.0037 0.0013 0.01 0.002
Lipid Particles 0.0013 0.0001 0.0003 0.0001 0.0042 0.0006 0.0136 0.0001 0.0044 0.0334 0.0109 0.0044 0.0098 0.0016 0.0082 0.0136 0.0073 0.0042
Mitochondria 0.0009 0.0005 0.0003 0.0001 0.0018 0.0004 0.025 0.0005 0.0076 0.0149 0.0035 0.0072 0.0017 0.0004 0.0025 0.0003 0.0026 0.0022
None 0.7824 0.6742 0.8313 0.7427 0.6824 0.7134 0.705 0.799 0.782 0.6222 0.7351 0.7634 0.7409 0.7564 0.7782 0.8444 0.7646 0.7009
Nuclear Periphery 0.0009 0.0029 0.0004 0.0003 0.0003 0.0004 0.0065 0.0003 0.0023 0.0015 0.0082 0.0009 0.0028 0.0006 0.0012 0.0004 0.0031 0.0008
Nucleolus 0.0003 0.0002 0.0015 0 0.0001 0.0002 0.0029 0.0002 0.0008 0.0016 0.0039 0.0003 0.0005 0.0002 0.0011 0.0001 0.0006 0.0005
Nucleus 0.0012 0.002 0.0066 0.0006 0.0033 0.0019 0.0066 0.0019 0.0035 0.0011 0.0066 0.0019 0.0013 0.0011 0.0013 0.0005 0.0038 0.0017
Peroxisomes 0.0011 0.0001 0.0001 0.0001 0.0396 0.0003 0.0329 0.0002 0.008 0.0331 0.0097 0.0063 0.0171 0.0034 0.0085 0.0056 0.0067 0.0032
Punctate Nuclear 0.0016 0.0005 0.0021 0.0002 0.0009 0.0025 0.0109 0.0012 0.0046 0.0024 0.0192 0.0047 0.0044 0.0039 0.0038 0.0011 0.0037 0.0058
Vacuole 0.0009 0.0011 0.0004 0.0003 0.0003 0.0004 0.002 0.0007 0.0013 0.0085 0.0054 0.0005 0.0017 0.0005 0.0024 0.0002 0.001 0.0021
Vacuole Periphery 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0013 0.0001 0.0005 0.0018 0.001 0.0005 0.0007 0.0001 0.0004 0.0001 0.0004 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.7092 36.8319 31.3305 47.7148 31.2702 58.2532 46.3238 48.1242 53.921 33.8725
Translational Efficiency 1.5566 1.6533 1.9062 1.7421 1.4045 1.6902 1.5492 1.518 1.6898 1.4821

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YML131W

YML131W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml131w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available