ORF | |
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Human Ortholog | |
Description | Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 0 | 2 | 2 |
Bud | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 6 | 6 | 6 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 7 | 4 | 14 | 6 | 8 | 19 | 8 | 15 | 3 | 11 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 5 | 3 | 0 | 5 | 1 | 4 | 5 | 1 | 0 | 1 | 1 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 3 | 2 |
Cytoplasm | 155 | 169 | 179 | 335 | 407 | 387 | 549 | 568 | 403 | 432 | 219 | 289 | 237 | 202 | 294 | 97 | 91 | 62 | 113 | 150 | 144 | 96 | 246 | 275 |
Endoplasmic Reticulum | 1 | 1 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 6 | 4 | 3 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 0 | 1 | 1 |
Mitochondria | 25 | 3 | 0 | 3 | 6 | 22 | 72 | 86 | 132 | 95 | 135 | 87 | 0 | 0 | 1 | 2 | 2 | 3 | 16 | 10 | 17 | 3 | 11 | 10 |
Nucleus | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 1 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 3 | 4 | 10 | 12 | 2 | 6 | 3 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 5 |
Vac/Vac Membrane | 1 | 0 | 0 | 2 | 6 | 6 | 13 | 11 | 2 | 6 | 4 | 6 | 1 | 0 | 0 | 1 | 5 | 0 | 3 | 4 | 1 | 1 | 0 | 5 |
Unique Cell Count | 164 | 172 | 182 | 341 | 420 | 405 | 603 | 637 | 476 | 508 | 306 | 353 | 238 | 202 | 295 | 100 | 97 | 65 | 163 | 178 | 181 | 110 | 277 | 322 |
Labelled Cell Count | 186 | 174 | 194 | 355 | 443 | 438 | 662 | 719 | 567 | 568 | 374 | 408 | 244 | 202 | 297 | 113 | 108 | 71 | 163 | 178 | 181 | 110 | 277 | 322 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.3 | 4.6 | 4.4 | 4.6 | 4.9 | 4.1 | 4.1 | 3.3 | 3.5 | 2.9 | 3.4 | 5.0 | 5.0 | 4.8 | 5.3 | 6.4 | 5.7 | 4.4 | 4.6 | 5.1 |
Std Deviation (1e-4) | 1.2 | 1.2 | 1.2 | 1.7 | 1.6 | 2.7 | 1.9 | 1.6 | 1.0 | 1.3 | 0.8 | 1.0 | 1.5 | 1.2 | 1.5 | 1.7 | 2.8 | 2.9 | 1.8 | 1.1 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.0 | 0.07 | -0.15 | -0.19 | -0.5 | -0.38 | -0.65 | -0.42 | 0.11 | 0.11 | 0.06 | 0.2 | 0.48 | 0.31 | -0.07 | -0.01 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0095 | 0.5284 | 0.0111 | 0.0785 | -0.0376 | 0.3441 | 0.092 | 0.4649 | 0.2851 | 0.6418 | 0.2529 | 0.2437 | -1.1278 | -1.1354 | -1.3388 | -1.0302 | -1.3891 | -0.9816 |
Actin | 0.0185 | 0.0004 | 0.0004 | 0.0002 | 0.0068 | 0.0066 | 0.0365 | 0.0018 | 0.017 | 0.0056 | 0.0179 | 0.0075 | 0.0453 | 0.0011 | 0.007 | 0.0005 | 0.0508 | 0.0026 |
Bud | 0.0044 | 0.01 | 0.004 | 0 | 0.0004 | 0.0006 | 0.0049 | 0.0007 | 0.0045 | 0.0004 | 0.0061 | 0.0005 | 0.0016 | 0.0024 | 0.001 | 0.0001 | 0.0093 | 0.0012 |
Bud Neck | 0.0002 | 0.0002 | 0.0039 | 0.0001 | 0.0002 | 0.0061 | 0.0038 | 0.0003 | 0.0025 | 0.0018 | 0.0015 | 0.0025 | 0.006 | 0.0001 | 0.0008 | 0.0001 | 0.0052 | 0.0067 |
Bud Periphery | 0.0021 | 0.0051 | 0.0014 | 0 | 0.0005 | 0.0005 | 0.0059 | 0.0019 | 0.005 | 0.0007 | 0.004 | 0.0008 | 0.0032 | 0.0024 | 0.0009 | 0.0001 | 0.0124 | 0.0016 |
Bud Site | 0.0036 | 0.0071 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0065 | 0.0023 | 0.0111 | 0.0038 | 0.0065 | 0.0005 | 0.0183 | 0.0089 | 0.0098 | 0.0002 | 0.0166 | 0.0049 |
Cell Periphery | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.1615 | 0.2733 | 0.1419 | 0.2521 | 0.2486 | 0.2548 | 0.0976 | 0.1807 | 0.1275 | 0.1766 | 0.1136 | 0.1815 | 0.0903 | 0.2083 | 0.1408 | 0.1196 | 0.0795 | 0.237 |
Cytoplasmic Foci | 0.0128 | 0.0046 | 0.003 | 0.0019 | 0.0071 | 0.0083 | 0.0169 | 0.0059 | 0.0075 | 0.0442 | 0.0203 | 0.0091 | 0.0313 | 0.0058 | 0.0163 | 0.0098 | 0.0121 | 0.0136 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0053 | 0.0005 | 0.0006 | 0.0006 | 0.0007 | 0.0024 | 0.0006 | 0.0017 | 0.0008 | 0.0018 | 0.0006 | 0.002 | 0.0012 | 0.0019 | 0.0006 | 0.0022 | 0.0009 |
Endosome | 0.0024 | 0.0113 | 0.0007 | 0.0004 | 0.001 | 0.0017 | 0.0124 | 0.0011 | 0.0053 | 0.0377 | 0.0207 | 0.0045 | 0.015 | 0.0013 | 0.0097 | 0.0013 | 0.0075 | 0.0065 |
Golgi | 0.0015 | 0.0003 | 0.0001 | 0 | 0.0016 | 0.0002 | 0.0057 | 0.0001 | 0.0023 | 0.0075 | 0.0029 | 0.0023 | 0.0051 | 0.0003 | 0.0037 | 0.0013 | 0.01 | 0.002 |
Lipid Particles | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0042 | 0.0006 | 0.0136 | 0.0001 | 0.0044 | 0.0334 | 0.0109 | 0.0044 | 0.0098 | 0.0016 | 0.0082 | 0.0136 | 0.0073 | 0.0042 |
Mitochondria | 0.0009 | 0.0005 | 0.0003 | 0.0001 | 0.0018 | 0.0004 | 0.025 | 0.0005 | 0.0076 | 0.0149 | 0.0035 | 0.0072 | 0.0017 | 0.0004 | 0.0025 | 0.0003 | 0.0026 | 0.0022 |
None | 0.7824 | 0.6742 | 0.8313 | 0.7427 | 0.6824 | 0.7134 | 0.705 | 0.799 | 0.782 | 0.6222 | 0.7351 | 0.7634 | 0.7409 | 0.7564 | 0.7782 | 0.8444 | 0.7646 | 0.7009 |
Nuclear Periphery | 0.0009 | 0.0029 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0065 | 0.0003 | 0.0023 | 0.0015 | 0.0082 | 0.0009 | 0.0028 | 0.0006 | 0.0012 | 0.0004 | 0.0031 | 0.0008 |
Nucleolus | 0.0003 | 0.0002 | 0.0015 | 0 | 0.0001 | 0.0002 | 0.0029 | 0.0002 | 0.0008 | 0.0016 | 0.0039 | 0.0003 | 0.0005 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0005 |
Nucleus | 0.0012 | 0.002 | 0.0066 | 0.0006 | 0.0033 | 0.0019 | 0.0066 | 0.0019 | 0.0035 | 0.0011 | 0.0066 | 0.0019 | 0.0013 | 0.0011 | 0.0013 | 0.0005 | 0.0038 | 0.0017 |
Peroxisomes | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0396 | 0.0003 | 0.0329 | 0.0002 | 0.008 | 0.0331 | 0.0097 | 0.0063 | 0.0171 | 0.0034 | 0.0085 | 0.0056 | 0.0067 | 0.0032 |
Punctate Nuclear | 0.0016 | 0.0005 | 0.0021 | 0.0002 | 0.0009 | 0.0025 | 0.0109 | 0.0012 | 0.0046 | 0.0024 | 0.0192 | 0.0047 | 0.0044 | 0.0039 | 0.0038 | 0.0011 | 0.0037 | 0.0058 |
Vacuole | 0.0009 | 0.0011 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.002 | 0.0007 | 0.0013 | 0.0085 | 0.0054 | 0.0005 | 0.0017 | 0.0005 | 0.0024 | 0.0002 | 0.001 | 0.0021 |
Vacuole Periphery | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0005 | 0.0018 | 0.001 | 0.0005 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0013 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.7092 | 36.8319 | 31.3305 | 47.7148 | 31.2702 | 58.2532 | 46.3238 | 48.1242 | 53.921 | 33.8725 |
Translational Efficiency | 1.5566 | 1.6533 | 1.9062 | 1.7421 | 1.4045 | 1.6902 | 1.5492 | 1.518 | 1.6898 | 1.4821 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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