Standard name
Human Ortholog
Description Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0 – 0.07 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0.07 0.05 0.12 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.67 0.43 0.7 0.58 0.7 0.32 0.3 0.26 – 0.19 0.12 0.15 0.73 0.67 0.76 0.68 0.64 0.65 0.84 0.76 0.77 0.83 0.76 0.81
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0.06 0.06
Mitochondria 0.18 0.34 0.08 0.23 0.19 0.49 0.46 0.43 – 0.59 0.69 0.63 0 0.05 0 0.08 0.11 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.29 0.2 0.24 0.14 0.14 0.14 0.16 – 0.11 0.1 0.09 0.19 0.15 0.13 0.14 0.16 0.15 0 0 0 0 0 0
Peroxisomes 0.06 0 0.3 0.17 0.16 0.13 0.18 0.18 – 0.15 0.09 0.09 0.05 0.05 0 0 0 0 0 0 0.05 0 0.05 0
SpindlePole 0.18 0.12 0.39 0.35 0.21 0.18 0.22 0.22 – 0.18 0.15 0.19 0.24 0.24 0.19 0.25 0.22 0.23 0.06 0.05 0.12 0.06 0 0
Vac/Vac Membrane 0.16 0.19 0 0 0.07 0 0 0 – 0 0 0.06 0.21 0.18 0.2 0.07 0.12 0.15 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 4 – 2 5 3 0 2 0 3 2 1 1 3 2 1 6 5
Bud 1 4 3 9 9 15 32 22 – 39 45 27 0 3 0 6 1 0 2 3 1 0 2 0
Bud Neck 0 0 1 3 0 0 0 1 – 2 0 2 0 0 0 0 0 0 0 2 0 0 1 0
Bud Site 0 0 1 0 0 2 0 4 – 1 9 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 2 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 6 10 10 6 20 19 23 13 – 9 14 10 4 15 11 25 32 20 1 3 1 0 0 1
Endoplasmic Reticulum 1 0 1 0 0 0 4 2 – 0 1 2 0 2 1 5 6 4 0 0 0 0 5 2
Endosome 267 172 347 142 306 152 164 136 – 113 55 67 113 146 172 147 180 154 356 355 392 255 342 285
Golgi 3 0 1 0 1 0 0 0 – 2 0 4 0 8 3 10 9 15 10 8 10 7 25 22
Mitochondria 73 137 41 56 83 233 246 225 – 344 330 274 1 10 1 18 32 20 6 11 4 4 14 7
Nucleus 3 1 3 3 0 2 1 3 – 1 1 2 3 1 0 1 0 1 0 2 0 0 0 0
Nuclear Periphery 1 8 2 0 4 0 2 1 – 6 0 5 4 3 2 0 1 2 1 2 1 2 4 1
Nucleolus 122 115 98 58 62 68 78 84 – 65 46 39 30 33 29 30 46 35 1 9 2 2 1 0
Peroxisomes 24 6 150 43 69 63 96 93 – 87 44 38 7 11 6 8 12 7 7 8 26 9 22 13
SpindlePole 70 47 194 85 92 87 116 113 – 105 73 82 37 53 43 54 62 55 24 24 60 18 9 2
Vac/Vac Membrane 65 74 20 5 30 20 11 21 – 11 7 28 33 40 46 16 34 35 8 22 2 4 11 4
Unique Cell Count 397 398 498 246 435 471 538 518 585 475 438 154 218 225 217 281 238 426 466 510 310 453 352
Labelled Cell Count 636 574 873 410 676 661 773 722 789 630 584 232 327 314 324 418 349 426 466 510 310 453 352


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 7.6 7.0 6.6 6.6 5.7 5.5 6.1 – 5.5 5.6 6.9 8.8 8.9 9.1 7.6 8.2 9.4 8.7 9.7 10.6
Std Deviation (1e-4) 1.6 1.6 1.2 1.3 1.6 1.4 1.2 1.2 – 1.1 1.1 3.3 1.7 2.0 1.7 1.6 1.9 2.1 1.8 2.4 2.8
Intensity Change (Log2) – – – -0.1 -0.09 -0.3 -0.35 -0.22 – -0.35 -0.34 -0.03 0.32 0.34 0.37 0.12 0.23 0.41 0.31 0.47 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 2.0 3.0 4.8 3.7 – 5.2 6.4 4.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 2.2 1.8 2.1 0.5 – -0.6 0.9 0.3 0 3.3 2.1 5.4 5.6 4.1
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -3.2 0.2 -11.6 -12.6 -13.9 – -16.7 -18.4 -16.7 0.9 -0.7 1.9 -0.5 -1.6 -1.4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 5.3
Mitochondria 5.5 4.9 14.2 13.5 12.8 – 17.3 19.7 17.6 -3.4 -1.7 -4.1 0 1.5 0.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 -2.2 -2.2 -2.2 -1.4 – -3.9 -4.4 -4.7 -0.1 -1.4 -2.2 -1.9 -1.1 -1.6
Peroxisomes -3.7 -5.1 -6.3 -4.6 -4.5 – -6.0 -8.1 -8.2 -6.5 -7.4 -8.3 -7.8 -8.5 -8.4
SpindlePole -1.2 -5.9 -7.0 -6.1 -5.9 – -7.7 -8.2 -6.8 -3.4 -3.8 -5.3 -3.6 -4.8 -4.3
Vacuole -1.4 1.9 0.2 -1.9 0 – -2.1 -2.4 1.6 6.9 6.4 7.1 1.9 4.3 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1836 4.6058 4.5684 3.8162 3.5955 4.2462 5.8519 7.8237 7.0451 6.4777 6.3119 7.3761 5.96 7.5048 6.9596 6.3025 6.4501 7.0853
Actin 0.0126 0.005 0.0345 0.0001 0.0371 0.0053 0.0009 0.0017 0.0002 0 0 0.0001 0.0001 0.0001 0.003 0.0001 0.0001 0.0001
Bud 0.0003 0.0056 0.0012 0.0003 0.0084 0.0002 0 0.0006 0.0001 0 0 0 0 0 0.0001 0 0 0.0001
Bud Neck 0.0011 0.0009 0.0023 0.0001 0.001 0.0014 0.0002 0.0004 0.0003 0.0001 0.0001 0.0002 0.0003 0.0003 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.0005 0.0012 0.002 0.0003 0.0184 0.0003 0 0.0005 0.0001 0 0 0 0 0 0 0.0001 0 0
Bud Site 0.0017 0.0039 0.0057 0.0002 0.0209 0.0008 0.0001 0.0041 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0002 0.0001 0 0.0001
Cell Periphery 0.0003 0.0003 0.0007 0 0.0007 0.0002 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0
Cytoplasm 0.01 0.0057 0.0067 0.0022 0.0038 0.0051 0.0006 0.0009 0.0006 0.0004 0.0004 0.0003 0.0007 0.0008 0.0008 0.0005 0.0005 0.0006
Cytoplasmic Foci 0.1444 0.1712 0.1514 0.106 0.1328 0.1286 0.1771 0.1968 0.1878 0.179 0.1777 0.1468 0.1661 0.1986 0.1806 0.1654 0.185 0.1412
Eisosomes 0.0006 0 0.0006 0 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0059 0.0008 0.0243 0.0005 0.0026 0.0021 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Endosome 0.4069 0.4855 0.3863 0.6737 0.4943 0.4461 0.401 0.3998 0.43 0.531 0.5337 0.3325 0.4485 0.4421 0.4611 0.5305 0.5259 0.3765
Golgi 0.0473 0.0588 0.0953 0.0771 0.0821 0.096 0.0945 0.1202 0.126 0.1027 0.1062 0.1138 0.0995 0.0924 0.1278 0.1445 0.1651 0.124
Lipid Particles 0.1925 0.1167 0.1829 0.0556 0.0897 0.2337 0.2271 0.1834 0.1819 0.1238 0.1141 0.3141 0.2075 0.209 0.1452 0.116 0.094 0.2949
Mitochondria 0.0198 0.0019 0.0081 0.0023 0.0045 0.004 0.0039 0.0142 0.003 0.0023 0.0009 0.0021 0.0012 0.0009 0.0013 0.0091 0.0006 0.0028
None 0.007 0.0002 0.0134 0.0133 0.0017 0.0107 0.0002 0.0003 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0
Nuclear Periphery 0.0469 0.0024 0.0015 0.0012 0.0132 0.0023 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0007 0.0001 0.0002
Nucleolus 0.0051 0.0017 0.0035 0.0016 0.0006 0.0094 0.0002 0.0006 0.0002 0.0004 0 0.0002 0.0014 0.001 0.0001 0.0006 0.0001 0.0004
Nucleus 0.0139 0.0009 0.0008 0.0017 0.0022 0.0016 0.0001 0.0002 0.0001 0 0 0 0.0003 0.0002 0.0001 0.0001 0 0.0001
Peroxisomes 0.0389 0.022 0.0443 0.0035 0.0163 0.0298 0.0884 0.0656 0.0627 0.045 0.0628 0.0847 0.0621 0.0305 0.0633 0.0163 0.0242 0.0486
Punctate Nuclear 0.0105 0.0022 0.0097 0.0346 0.0076 0.0021 0.0014 0.001 0.0009 0.0004 0.0004 0.0008 0.0048 0.0006 0.0024 0.0003 0.0004 0.002
Vacuole 0.0233 0.0938 0.0202 0.0182 0.0433 0.0153 0.0024 0.0066 0.0037 0.0085 0.0019 0.0024 0.0047 0.0175 0.0095 0.0068 0.0022 0.005
Vacuole Periphery 0.0105 0.0194 0.0049 0.0074 0.0185 0.005 0.0015 0.0027 0.0016 0.006 0.0015 0.0017 0.002 0.0053 0.0037 0.0086 0.0017 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.5304 22.476 28.1146 22.9434 16.765 25.1288 32.9519 25.8558 25.5061 24.3663
Translational Efficiency 1.0848 1.3538 0.9554 1.2791 1.4236 1.4887 1.0054 1.1708 1.2635 1.2009

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages endosome (26%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mvp1

Mvp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mvp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available