Standard name
Human Ortholog
Description High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.33 0.27 0.28 0 0 0 0 0 0 0.07 0 0.19 0.2 0.16 0 0.09 – 0 0.05 0 0 0 0
Bud 0 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0.64 0.69 0.7 0.61 0.13 0.05 0 0 0.09 0.05 0.18 0.09 0.74 0.73 0.6 0.46 0.61 – 0.51 0.42 0.63 0.58 0.59 0.71
Cytoplasm 0.06 0.22 0.1 0.12 0.13 0.19 0.11 0.18 0.28 0.27 0.39 0.33 0.06 0.06 0.08 0.17 0.28 – 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.36 0.14 0.09 0.15 0.29 0.09 0.06 0 0.07 0.07 0.11 0.06 0.12 0.13 0.14 0 0.06 – 0.28 0.22 0.14 0.18 0.17 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.08 0 0.05 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.11 – 0 0 0.07 0.07 0 0.06
Nucleus 0 0 0 0 0.05 0.12 0.22 0.22 0.11 0.15 0.07 0.13 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.46 0.62 0.66 0.68 0.51 0.57 0.34 0.48 0 0 0.12 0.42 0.06 – 0.12 0.15 0.06 0.1 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 17 80 62 8 17 10 13 14 16 29 10 165 177 153 0 9 – 0 4 11 8 15 9
Bud 2 3 15 14 1 2 3 8 8 3 15 16 32 32 32 1 4 – 0 3 9 12 25 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 3 2 11 7 18 7
Bud Site 0 1 2 0 0 0 0 0 0 0 1 0 3 4 3 0 0 – – – – – – –
Cell Periphery 58 35 208 134 48 22 14 9 45 36 80 57 642 642 572 11 63 – 60 38 244 249 379 494
Cytoplasm 5 11 30 26 49 86 76 170 145 182 172 204 56 56 80 4 29 – 5 5 5 3 24 6
Endoplasmic Reticulum 32 7 26 33 111 43 42 23 36 44 48 35 100 119 138 0 6 – 33 20 54 75 109 90
Endosome 0 0 2 0 0 6 5 2 2 7 0 3 0 0 0 0 0 – 0 0 0 0 1 0
Golgi 1 0 6 7 8 6 6 4 8 2 3 6 60 61 72 1 5 – 0 0 0 0 0 0
Mitochondria 0 0 11 4 5 15 17 20 14 35 13 27 1 1 2 0 11 – 1 1 25 29 24 43
Nucleus 1 0 4 1 19 53 154 212 56 102 31 79 5 5 4 0 0 – 0 0 0 0 0 1
Nuclear Periphery 0 0 1 0 0 0 5 7 1 4 1 6 2 2 4 0 0 – 0 0 0 0 0 0
Nucleolus 1 0 1 4 4 2 5 11 12 7 5 10 1 1 2 0 1 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 2 4 0 2 2 2 2 2 4 1 40 40 47 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 2 0 10 6 175 283 457 662 260 381 150 295 36 37 114 10 6 – 13 13 23 40 40 24
Unique Cell Count 90 51 299 221 380 460 689 968 509 665 437 613 867 882 959 24 104 120 92 389 428 645 699
Labelled Cell Count 115 74 398 295 428 537 796 1143 604 822 552 749 1143 1177 1224 27 134 120 92 389 428 645 699


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.4 22.3 29.9 35.5 30.9 25.0 23.1 20.6 23.3 20.5 24.6 23.0 27.4 27.4 21.5 25.6 41.4 – 30.9 33.6 40.5
Std Deviation (1e-4) 13.2 7.6 15.2 16.6 14.0 14.1 12.5 10.6 11.4 10.1 11.1 10.3 10.4 10.4 8.4 15.5 23.5 – 14.5 14.8 17.5
Intensity Change (Log2) – – – 0.25 0.05 -0.25 -0.37 -0.53 -0.36 -0.54 -0.28 -0.38 -0.12 -0.12 -0.48 -0.22 0.47 – 0.05 0.17 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -9.5 -9.3 -12.7 -14.7 -10.3 -11.7 -7.5 -11.9 -2.8 -2.4 -4.2 -2.9 -3.8 –
Bud 0.6 -4.1 -4.2 -4.9 -4.7 -2.8 -4.8 -1.1 -1.9 -1.0 -1.1 -1.3 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery -2.1 -15.2 -19.0 -23.4 -27.5 -18.0 -21.2 -14.0 -18.8 1.5 1.1 -3.1 -2.4 -1.7 –
Cytoplasm 0.6 1.2 3.2 0.5 3.1 6.1 6.0 8.8 7.5 -2.0 -2.1 -0.9 0 4.4 –
Endoplasmic Reticulum 2.2 6.6 0.3 -1.5 -5.0 -0.8 -1.2 1.0 -1.7 1.4 2.2 2.6 0 -1.0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0.8 0.1 0 0 0 -0.5 0 0 0 3.2 3.2 3.4 0 0 –
Mitochondria -1.3 -2.0 -0.3 -1.1 -1.6 -0.7 1.1 -0.5 0.5 0 0 0 0 2.7 –
Nucleus 0 2.6 5.2 8.3 8.3 5.1 6.4 3.6 5.7 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 3.2 3.1 3.3 0 0 –
Vacuole -0.4 12.4 16.1 18.2 19.7 13.9 15.8 10.0 13.5 0.6 0.7 4.3 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.5337 34.4499 30.522 24.9328 22.4546 31.1948 27.9065 45.1498 40.4012 35.1347 29.7046 40.5644 26.5554 37.8704 28.8234 22.3066 25.6503 37.4909
Actin 0.0216 0.0001 0.0015 0.0081 0.0018 0.0011 0.0044 0.0003 0.0031 0.0005 0.0149 0.0018 0.0047 0.0035 0.0014 0.0002 0.0012 0.0068
Bud 0.0026 0.0022 0.0012 0.0017 0.0031 0.002 0.0011 0.0011 0.0008 0.0003 0.0007 0.0006 0.0007 0.0011 0.0015 0.001 0.0011 0.001
Bud Neck 0.0021 0.0004 0.0011 0.0014 0.0019 0.0304 0.0013 0.0007 0.0005 0.0004 0.0009 0.0054 0.0017 0.0022 0.0014 0.0045 0.0006 0.0279
Bud Periphery 0.0149 0.0056 0.0091 0.0307 0.0151 0.0144 0.0081 0.0056 0.007 0.0068 0.0039 0.0106 0.0053 0.0048 0.0118 0.0134 0.0268 0.0186
Bud Site 0.0123 0.0077 0.0099 0.0104 0.0024 0.0014 0.0066 0.0241 0.0047 0.0004 0.001 0.0015 0.0084 0.0531 0.0143 0.0036 0.0016 0.0007
Cell Periphery 0.5834 0.6301 0.6388 0.5867 0.5203 0.6141 0.6751 0.7643 0.7573 0.7582 0.6886 0.7872 0.7166 0.7401 0.7442 0.7296 0.6771 0.7163
Cytoplasm 0.0173 0.0124 0.0109 0.0072 0.0159 0.0103 0.009 0.0039 0.0063 0.0011 0.0019 0.0026 0.0112 0.0042 0.0068 0.0034 0.0027 0.0037
Cytoplasmic Foci 0.0087 0.0001 0.0005 0.0015 0.0048 0.0012 0.006 0 0.0109 0.0006 0.0048 0.0036 0.0145 0.0001 0.0095 0.0001 0.0218 0.0009
Eisosomes 0.0024 0.0006 0.0009 0.0007 0.0001 0.0002 0.0105 0.0012 0.0021 0.0013 0.0016 0.003 0.0049 0.006 0.0011 0.0007 0.0001 0.0008
Endoplasmic Reticulum 0.0213 0.0211 0.0341 0.018 0.0064 0.0269 0.0164 0.0122 0.0187 0.0182 0.0062 0.0113 0.0153 0.015 0.0217 0.0352 0.0309 0.0148
Endosome 0.015 0.0003 0.0013 0.0108 0.021 0.0003 0.0115 0.0001 0.0017 0.001 0.0181 0.0006 0.0041 0.0001 0.0029 0.0003 0.0091 0.0068
Golgi 0.003 0 0.0006 0.0014 0.0048 0.0002 0.0082 0 0.0012 0.0005 0.0048 0.0006 0.002 0.0001 0.0009 0.0001 0.0055 0.0025
Lipid Particles 0.0204 0.0157 0.0099 0.0061 0.0365 0.0076 0.0154 0.004 0.0047 0.0104 0.0048 0.0048 0.0225 0.0031 0.0037 0.0048 0.0215 0.0067
Mitochondria 0.0071 0.0002 0.002 0.0016 0.0014 0.0002 0.0359 0.0002 0.0087 0.0413 0.0249 0.0136 0.0108 0.0003 0.0008 0.0003 0.0009 0.0008
None 0.0054 0.0001 0.0005 0.0039 0.0007 0.0014 0.0161 0.0001 0.0004 0.0004 0.0122 0.0003 0.0083 0.0002 0.011 0.0001 0.0004 0.0003
Nuclear Periphery 0.0158 0.0049 0.007 0.0106 0.0033 0.0032 0.007 0.008 0.0076 0.0027 0.0145 0.0061 0.0095 0.0092 0.0093 0.0035 0.0054 0.0054
Nucleolus 0.0032 0.0004 0.0011 0.0033 0.0008 0.0103 0.0061 0.0004 0.0006 0.0007 0.034 0.0009 0.001 0.0007 0.0013 0.0013 0.0004 0.0006
Nucleus 0.0039 0.0006 0.0011 0.003 0.0013 0.0027 0.0018 0.0005 0.0007 0.0005 0.007 0.0008 0.0008 0.0007 0.0016 0.0012 0.0005 0.0008
Peroxisomes 0.003 0 0.0008 0.0003 0.0021 0 0.007 0 0.0014 0.001 0.0011 0.0013 0.0078 0.0001 0.0013 0 0.0318 0.0004
Punctate Nuclear 0.0053 0 0.0003 0.002 0.0017 0.0005 0.0089 0 0.0005 0 0.0015 0.0017 0.0004 0 0.002 0 0.0004 0.0003
Vacuole 0.2262 0.2955 0.2635 0.2838 0.3487 0.2693 0.1398 0.1716 0.157 0.149 0.1468 0.1389 0.1476 0.1543 0.1487 0.1935 0.1557 0.1812
Vacuole Periphery 0.0053 0.0018 0.0038 0.0069 0.0059 0.0022 0.004 0.0016 0.0041 0.0045 0.0057 0.0026 0.0021 0.0011 0.0027 0.0033 0.0046 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 244.6355 165.0027 216.9693 455.479 211.6004 264.5077 213.6027 207.6094 393.0724 386.2127
Translational Efficiency 1.4079 1.3977 1.3619 1.2379 1.4411 1.8354 1.501 1.2195 1.3431 1.2656

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose
Localization
Cell Percentages cell periphery (16%), cytoplasm (7%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hxt2

Hxt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hxt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available