Standard name | |
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Human Ortholog | |
Description | Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | – | 1 | 2 | 2 | 4 | – | 1 | – | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | – | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 7 | 0 | – | 0 | 1 | 3 | 2 | – | 3 | – | – | 1 | 11 | 7 | 8 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 409 | 219 | 57 | – | 30 | 21 | 27 | 41 | – | 11 | – | – | 169 | 213 | 218 | 227 | 93 | 121 | 415 | 219 | 68 | 100 | 252 | 230 |
Endoplasmic Reticulum | 26 | 8 | 2 | – | 2 | 3 | 6 | 8 | – | 3 | – | – | 4 | 4 | 3 | 10 | 3 | 4 | 9 | 1 | 6 | 6 | 21 | 28 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Golgi | 2 | 0 | 0 | – | 0 | 0 | 2 | 3 | – | 0 | – | – | 0 | 0 | 0 | 10 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 6 |
Mitochondria | 0 | 1 | 1 | – | 2 | 3 | 3 | 12 | – | 5 | – | – | 1 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 3 | 0 | 1 | 0 |
Nucleus | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 2 | – | 4 | 0 | 2 | 6 | – | 0 | – | – | 1 | 0 | 1 | 10 | 5 | 3 | 7 | 8 | 5 | 5 | 9 | 9 |
Unique Cell Count | 436 | 227 | 63 | 37 | 28 | 38 | 69 | 19 | 174 | 224 | 228 | 250 | 107 | 126 | 450 | 235 | 85 | 117 | 295 | 284 | ||||
Labelled Cell Count | 452 | 237 | 64 | 39 | 31 | 46 | 77 | 23 | 176 | 229 | 233 | 269 | 112 | 135 | 450 | 235 | 85 | 117 | 295 | 284 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 41.6 | 41.8 | 20.3 | – | 16.3 | 19.3 | 19.3 | 14.1 | – | 11.8 | – | – | 40.3 | 37.3 | 32.0 | 36.9 | 39.7 | 39.9 | 40.2 | 45.0 | 47.0 |
Std Deviation (1e-4) | 10.6 | 9.8 | 4.5 | – | 5.7 | 3.7 | 4.4 | 5.1 | – | 3.7 | – | – | 7.5 | 7.9 | 6.7 | 12.1 | 12.6 | 13.9 | 8.6 | 11.0 | 12.4 |
Intensity Change (Log2) | – | – | – | – | -0.32 | -0.07 | -0.08 | -0.53 | – | -0.78 | – | – | 0.99 | 0.88 | 0.65 | 0.86 | 0.97 | 0.97 | 0.98 | 1.14 | 1.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | -1.3 | -1.9 | -2.5 | -4.1 | – | -3.3 | – | – | 2.1 | 1.4 | 1.6 | 0.1 | -0.7 | 1.5 |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 0 | 3.0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | – | 0 | 0 | 0 | 0 | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 31.3447 | 44.9511 | 38.6422 | 35.9928 | 36.2822 | 37.1838 | 31.9282 | 47.5807 | 42.0363 | 40.0103 | 37.67 | 43.949 | 35.0319 | 50.7849 | 43.8175 | 44.7134 | 41.192 | 44.4928 |
Actin | 0.0041 | 0.0017 | 0.0065 | 0.0118 | 0.0055 | 0.0077 | 0.0153 | 0.0018 | 0.0074 | 0.0091 | 0.0317 | 0.0019 | 0.0142 | 0.0006 | 0.0012 | 0.0015 | 0.0014 | 0.0024 |
Bud | 0.002 | 0.0025 | 0.0028 | 0.0087 | 0.0042 | 0.002 | 0.0017 | 0.0006 | 0.0013 | 0.0039 | 0.0014 | 0.0009 | 0.0022 | 0.0004 | 0.0014 | 0.0017 | 0.0011 | 0.0008 |
Bud Neck | 0.0011 | 0.0015 | 0.0025 | 0.002 | 0.0059 | 0.0028 | 0.0082 | 0.0004 | 0.0008 | 0.0011 | 0.0009 | 0.0018 | 0.0006 | 0.0003 | 0.0006 | 0.0008 | 0.0005 | 0.0011 |
Bud Periphery | 0.0016 | 0.0016 | 0.0018 | 0.0044 | 0.0068 | 0.0016 | 0.0014 | 0.0004 | 0.0013 | 0.0039 | 0.0014 | 0.0003 | 0.0021 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0005 |
Bud Site | 0.0011 | 0.0025 | 0.0026 | 0.0016 | 0.001 | 0.0004 | 0.0099 | 0.0005 | 0.0012 | 0.0136 | 0.0006 | 0.0001 | 0.0015 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0037 | 0.0098 | 0.0084 | 0.0022 | 0.0081 | 0.0017 | 0.0011 | 0.0016 | 0.0013 | 0.0033 | 0.0003 | 0.0003 | 0.0045 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasm | 0.5968 | 0.8157 | 0.7015 | 0.527 | 0.4922 | 0.5772 | 0.7613 | 0.9529 | 0.8797 | 0.7162 | 0.7864 | 0.803 | 0.6565 | 0.9419 | 0.8558 | 0.8083 | 0.7513 | 0.7205 |
Cytoplasmic Foci | 0.0056 | 0.006 | 0.0078 | 0.0118 | 0.0091 | 0.0132 | 0.0147 | 0.0051 | 0.0101 | 0.0156 | 0.0125 | 0.0205 | 0.0388 | 0.0072 | 0.0133 | 0.02 | 0.0301 | 0.0225 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0797 | 0.0303 | 0.0292 | 0.0371 | 0.0082 | 0.065 | 0.0402 | 0.0059 | 0.0081 | 0.0082 | 0.0199 | 0.036 | 0.0215 | 0.0058 | 0.0084 | 0.0123 | 0.0067 | 0.0469 |
Endosome | 0.0476 | 0.0274 | 0.0395 | 0.064 | 0.114 | 0.0958 | 0.0461 | 0.0099 | 0.0317 | 0.0847 | 0.0527 | 0.0928 | 0.0886 | 0.0201 | 0.0596 | 0.0929 | 0.1237 | 0.1253 |
Golgi | 0.0065 | 0.0044 | 0.0107 | 0.0119 | 0.0234 | 0.0333 | 0.0064 | 0.0037 | 0.0096 | 0.01 | 0.0167 | 0.0074 | 0.0233 | 0.0043 | 0.0097 | 0.0125 | 0.0125 | 0.0319 |
Lipid Particles | 0.0009 | 0.0007 | 0.0016 | 0.0009 | 0.0005 | 0.0003 | 0.0021 | 0.0003 | 0.0014 | 0.0009 | 0.0009 | 0.0002 | 0.0098 | 0.0002 | 0.0038 | 0.0001 | 0.0005 | 0.0001 |
Mitochondria | 0.0046 | 0.0038 | 0.0037 | 0.0179 | 0.0045 | 0.0246 | 0.0178 | 0.0024 | 0.0099 | 0.0193 | 0.009 | 0.0023 | 0.0194 | 0.0107 | 0.0107 | 0.0035 | 0.0061 | 0.0153 |
None | 0.0008 | 0.0003 | 0.0006 | 0.0008 | 0.0004 | 0.0007 | 0.0047 | 0.0006 | 0.0018 | 0.0006 | 0.0007 | 0.0003 | 0.0034 | 0.0002 | 0.0009 | 0.0003 | 0.0021 | 0.0004 |
Nuclear Periphery | 0.0503 | 0.005 | 0.0109 | 0.0259 | 0.0226 | 0.0211 | 0.0181 | 0.001 | 0.0039 | 0.0035 | 0.0142 | 0.0036 | 0.015 | 0.0011 | 0.002 | 0.002 | 0.0049 | 0.0035 |
Nucleolus | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0 | 0.0006 | 0 | 0.0004 | 0 | 0.0004 | 0 |
Nucleus | 0.0096 | 0.0026 | 0.0064 | 0.0112 | 0.0048 | 0.0039 | 0.0066 | 0.0009 | 0.002 | 0.0018 | 0.0037 | 0.0011 | 0.0047 | 0.0005 | 0.001 | 0.0009 | 0.0036 | 0.0008 |
Peroxisomes | 0.0001 | 0.0001 | 0.0002 | 0.0014 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.0022 | 0 | 0.0013 | 0.0003 | 0.0003 | 0.0002 |
Punctate Nuclear | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0088 | 0.0001 | 0.0028 | 0.0004 | 0.0007 | 0.0001 | 0.0033 | 0 | 0.0002 | 0.0001 | 0.0013 | 0 |
Vacuole | 0.1192 | 0.065 | 0.1261 | 0.181 | 0.1941 | 0.0719 | 0.026 | 0.0103 | 0.0181 | 0.0711 | 0.0275 | 0.0138 | 0.0626 | 0.0042 | 0.0181 | 0.0259 | 0.0337 | 0.0134 |
Vacuole Periphery | 0.0644 | 0.0188 | 0.0368 | 0.0768 | 0.094 | 0.0766 | 0.0084 | 0.0015 | 0.006 | 0.0318 | 0.0176 | 0.0132 | 0.0252 | 0.0018 | 0.0106 | 0.0162 | 0.0188 | 0.014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 241.1719 | 201.1858 | 182.888 | 216.2579 | 192.7259 | 175.7451 | 198.5413 | 188.1208 | 177.7911 | 208.287 |
Translational Efficiency | 0.9175 | 1.0674 | 1.0358 | 0.8566 | 0.884 | 1.1821 | 0.9087 | 0.7695 | 0.8388 | 0.826 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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