Standard name
Human Ortholog
Description C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.12 0.1 0.08 0.06 0.05 0.11 0.11 0.07 0.14 0.05 0.05 0 0.11 0.09 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.09 0.05 0.05 0.07 0.05 0 0.09 0.08 0.08 0.12 0.12 0.18 0.19 0.17 0.16 0.21 0 0 0 0 0 0
Cytoplasm 0.08 0.08 0.08 0.08 0.08 0.1 0.12 0.12 0.14 0.11 0.21 0.17 0.07 0.09 0.23 0.38 0.34 0.34 0 0 0 0 0 0
Endoplasmic Reticulum 0.83 0.92 0.84 0.81 0.71 0.75 0.66 0.63 0.66 0.59 0.46 0.56 0.81 0.81 0.72 0 0 0 0.93 0.92 0.89 0.87 0.9 0.83
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.17 0.16 0 0 0 0 0 0
Mitochondria 0 0 0 0.11 0.08 0.08 0.09 0.09 0.13 0.17 0.17 0.21 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0 0 0.06 0.13 0.05 0.1 0.17 0 0.11 0.13 0.06 0.06 0 0 0.26 0.28 0.26 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 3 16 23 28 14 21 12 33 40 8 22 8 15 3 23 14 8 4 3 5 2 2 5
Bud 0 0 2 3 5 2 8 4 5 11 2 3 1 0 1 2 1 2 3 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 3 12 21 9 15 13 18 10 27 27 9 19 19 55 43 34 26 23 1 0 1 1 3 3
Cytoplasm 20 21 20 15 21 18 41 27 41 40 24 27 11 26 54 78 54 37 3 8 2 5 6 4
Endoplasmic Reticulum 216 253 200 162 198 138 229 142 196 210 52 90 125 246 166 6 3 1 267 270 230 163 199 188
Endosome 0 0 0 2 1 0 0 1 0 0 0 0 0 0 0 8 4 0 0 0 1 0 0 2
Golgi 4 1 1 9 15 3 5 0 0 3 0 0 0 0 0 8 27 18 0 0 2 2 2 2
Mitochondria 5 1 6 21 22 14 30 20 37 60 19 34 4 0 0 10 2 5 0 1 5 2 1 4
Nucleus 1 1 0 0 0 0 2 2 0 1 1 1 2 0 0 5 2 0 0 0 0 0 0 0
Nuclear Periphery 4 5 7 9 10 7 12 4 20 25 4 7 1 4 1 1 0 0 2 1 3 1 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 2 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 2 0 0 0 0 0 0
Vac/Vac Membrane 39 4 6 12 35 10 35 39 12 38 15 9 10 4 5 53 44 29 1 2 1 1 2 8
Unique Cell Count 261 274 237 199 277 184 345 225 295 353 112 162 155 302 232 203 160 110 289 293 258 187 222 226
Labelled Cell Count 300 301 279 265 350 219 402 261 371 455 135 213 181 350 273 237 180 128 289 293 258 187 222 226


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.1 24.0 14.1 14.9 16.3 14.5 12.1 12.2 12.1 11.8 10.9 11.2 16.5 15.5 10.7 12.2 15.1 16.5 19.1 20.7 21.6
Std Deviation (1e-4) 4.9 5.2 2.9 3.9 4.0 3.0 3.0 2.9 2.7 2.7 2.9 2.5 6.1 3.1 2.2 3.1 3.6 4.0 5.3 4.7 4.9
Intensity Change (Log2) – – – 0.08 0.21 0.04 -0.22 -0.21 -0.22 -0.26 -0.38 -0.33 0.23 0.13 -0.4 -0.21 0.09 0.23 0.43 0.55 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 1.4 0.3 -0.3 -0.6 1.8 1.9 0.1 2.3 -0.6 -0.9 -3.0 1.7 0.7 0.2
Bud 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -1.5 -0.7 -1.7 -1.9 0.1 -0.5 -0.3 0.9 1.1 3.1 3.1 2.5 2.2 3.1
Cytoplasm -0.3 -0.4 0.5 1.3 1.3 2.0 1.1 3.4 2.5 -0.5 0.1 4.4 7.5 6.4 5.9
Endoplasmic Reticulum -0.8 -3.5 -2.4 -4.8 -5.2 -4.7 -6.4 -7.4 -6.3 -1.0 -0.9 -3.4 -17.1 -16.1 -14.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 3.2 0 0 0 0 0 0 0 0 0 0 0 6.3 6.1
Mitochondria 3.5 2.7 2.4 3.0 3.0 4.2 5.5 4.9 6.0 0 0 0 1.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.9 0.4 0.5 0.3 -0.8 2.0 2.2 0 0.7 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 4.2 1.5 3.5 5.4 1.0 3.7 4.0 1.6 1.9 0 -0.3 7.2 7.4 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.6955 23.9802 21.4878 21.6558 22.1997 22.063 17.2831 21.655 19.7989 20.1821 19.4634 19.9841 5.68 21.4743 18.7623 19.877 21.6775 20.558
Actin 0.011 0.0027 0.0122 0.0002 0.0001 0.0015 0.0612 0.0003 0.0064 0.0008 0.0253 0.0005 0.2709 0.0001 0.0075 0.0198 0 0.0002
Bud 0.0004 0.0001 0.0002 0 0 0 0.001 0 0 0.0001 0.0003 0 0.0008 0 0.0001 0.0006 0 0
Bud Neck 0.0008 0.0006 0.0005 0.0005 0.0004 0.0008 0.0016 0.0002 0.0002 0.0005 0.0003 0.0006 0.0008 0 0.0006 0.0004 0.0001 0.0002
Bud Periphery 0.0008 0.0002 0.0005 0 0 0.0002 0.001 0.0002 0.0001 0.0004 0.0003 0 0.0014 0 0.0002 0.0016 0 0
Bud Site 0.0022 0.0013 0.001 0 0 0 0.0086 0.0002 0.0002 0 0.0007 0 0.0017 0 0.0005 0.0002 0 0
Cell Periphery 0.0011 0.0064 0.0084 0.0004 0.0015 0.0036 0.0023 0.01 0.0016 0.0016 0.0004 0.0005 0.0007 0.0003 0.0013 0.0035 0.0002 0.0003
Cytoplasm 0.0149 0.049 0.0196 0.0117 0.0021 0.0052 0.0277 0.0374 0.0246 0.0017 0.008 0.0034 0.0115 0.0019 0.0143 0.0068 0.0006 0.0018
Cytoplasmic Foci 0.0139 0.0009 0.0054 0.0001 0.0001 0.0007 0.0044 0.0004 0.0024 0.0001 0.0122 0.0012 0.007 0 0.0049 0.0004 0 0
Eisosomes 0.0004 0.0001 0.0002 0 0 0 0.0006 0 0 0.0003 0.0001 0 0.0025 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.7467 0.855 0.861 0.9706 0.9861 0.9364 0.7286 0.9167 0.9116 0.9708 0.8473 0.96 0.497 0.97 0.8961 0.9098 0.9881 0.9739
Endosome 0.0112 0.0027 0.0111 0.0007 0.0004 0.0025 0.028 0.0012 0.0094 0.0033 0.0294 0.009 0.0717 0.0003 0.0181 0.018 0.0004 0.0007
Golgi 0.0027 0.0011 0.0037 0.0002 0.0001 0.0037 0.0059 0.0003 0.0058 0.0015 0.0284 0.0044 0.024 0 0.002 0.0147 0.0001 0.0001
Lipid Particles 0.0121 0.0098 0.0123 0.0005 0.0006 0.0016 0.0053 0.0065 0.006 0.0011 0.0038 0.0019 0.0325 0.0001 0.0038 0.0008 0.0001 0.0001
Mitochondria 0.0091 0.0005 0.0196 0 0 0.0023 0.0083 0.0007 0.0007 0.002 0.0169 0.0005 0.0064 0.0001 0.0008 0.0083 0 0
None 0.0249 0.0001 0.0019 0 0 0 0.0086 0.0024 0.0014 0.0004 0.0002 0.0001 0.0018 0 0.004 0.0002 0 0
Nuclear Periphery 0.0982 0.0523 0.0274 0.0124 0.006 0.026 0.0802 0.0114 0.0246 0.0109 0.0205 0.0144 0.0449 0.0246 0.0306 0.0102 0.0083 0.0206
Nucleolus 0.0033 0.0001 0.0002 0 0 0 0.0009 0 0 0 0 0 0.0001 0 0.0003 0 0 0
Nucleus 0.0128 0.0065 0.0013 0.0007 0.0002 0.0015 0.0114 0.0004 0.0013 0.0004 0.0005 0.0004 0.0012 0.0006 0.003 0.0005 0.0002 0.0006
Peroxisomes 0.0075 0 0.0008 0 0 0.0003 0.0012 0 0.0004 0 0.0017 0 0.0011 0 0.0008 0 0 0
Punctate Nuclear 0.0134 0.0002 0.0012 0.0001 0 0.0001 0.0037 0.0001 0.0003 0 0.0003 0.0001 0.0009 0 0.007 0.0001 0 0
Vacuole 0.0078 0.0086 0.0092 0.0017 0.0022 0.0129 0.0059 0.0105 0.002 0.0025 0.0018 0.0025 0.0189 0.0012 0.0028 0.0023 0.0014 0.0009
Vacuole Periphery 0.0048 0.0018 0.0023 0.0002 0.0002 0.0007 0.0036 0.0011 0.0011 0.0015 0.0016 0.0005 0.0023 0.0007 0.0011 0.0016 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.9785 68.9304 88.7192 97.9357 64.5855 36.828 78.0219 81.3829 89.0911 81.8082
Translational Efficiency 1.3728 1.641 1.5258 1.3679 1.5085 1.7026 1.3169 1.3584 1.5064 1.394

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Erg5

Erg5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available