Standard name | |
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Human Ortholog | |
Description | C3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 34 | 10 | 3 | 13 | 18 | 6 | 9 | 11 | 3 | 10 | 9 | 23 | 26 | 10 | 2 | 4 | 0 | 15 | 56 | 11 | 6 | 28 | 24 |
Bud | 26 | 44 | 41 | 40 | 70 | 75 | 94 | 92 | 75 | 77 | 66 | 90 | 17 | 24 | 25 | 3 | 2 | 2 | 2 | 10 | 2 | 0 | 5 | 2 |
Bud Neck | 1 | 0 | 0 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 1 | 5 | 2 | 2 | 2 | 1 | 2 | 1 | 1 | 15 | 5 | 1 | 2 | 2 |
Bud Site | 11 | 17 | 31 | 14 | 40 | 61 | 30 | 37 | 34 | 37 | 24 | 39 | 4 | 8 | 1 | 4 | 6 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 20 | 0 | 3 | 0 | 1 | 2 | 2 | 2 | 2 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 20 | 16 | 28 | 20 | 25 | 24 | 30 | 22 | 19 | 14 | 19 | 13 | 5 | 7 | 6 | 0 | 2 | 6 | 7 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 2 | 3 | 2 | 0 | 4 | 8 | 5 | 5 | 2 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 0 |
Endosome | 43 | 43 | 27 | 33 | 29 | 13 | 21 | 24 | 28 | 38 | 22 | 12 | 20 | 30 | 65 | 9 | 10 | 5 | 49 | 101 | 46 | 28 | 33 | 28 |
Golgi | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 5 | 1 | 0 | 3 | 12 | 8 | 8 | 3 | 8 | 11 |
Mitochondria | 282 | 338 | 207 | 144 | 263 | 435 | 454 | 391 | 365 | 294 | 298 | 365 | 55 | 84 | 44 | 5 | 4 | 3 | 14 | 56 | 9 | 15 | 13 | 17 |
Nucleus | 0 | 4 | 2 | 2 | 4 | 4 | 2 | 6 | 5 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 4 | 6 | 4 | 0 | 7 | 13 | 7 | 9 | 12 | 3 | 8 | 1 | 3 | 2 | 4 | 4 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 11 | 8 | 10 | 5 | 13 | 17 | 12 | 19 | 20 | 19 | 17 | 15 | 1 | 1 | 4 | 1 | 3 | 3 | 0 | 1 | 0 | 1 | 1 | 0 |
Peroxisomes | 328 | 407 | 318 | 275 | 260 | 286 | 321 | 374 | 302 | 346 | 220 | 281 | 182 | 228 | 235 | 24 | 39 | 24 | 408 | 420 | 343 | 197 | 309 | 271 |
SpindlePole | 82 | 64 | 116 | 101 | 71 | 105 | 152 | 166 | 116 | 165 | 95 | 119 | 73 | 88 | 82 | 12 | 24 | 14 | 16 | 21 | 23 | 12 | 12 | 7 |
Vac/Vac Membrane | 11 | 3 | 1 | 1 | 2 | 1 | 1 | 3 | 3 | 1 | 2 | 1 | 0 | 1 | 1 | 2 | 2 | 2 | 0 | 6 | 1 | 3 | 0 | 1 |
Unique Cell Count | 494 | 625 | 447 | 386 | 480 | 623 | 713 | 745 | 596 | 608 | 486 | 579 | 264 | 331 | 323 | 58 | 66 | 45 | 533 | 717 | 457 | 278 | 427 | 376 |
Labelled Cell Count | 826 | 1013 | 785 | 650 | 800 | 1063 | 1132 | 1168 | 997 | 1006 | 782 | 962 | 403 | 506 | 485 | 74 | 98 | 65 | 533 | 717 | 457 | 278 | 427 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 5.4 | 4.9 | 4.9 | 4.4 | 4.5 | 4.0 | 4.4 | 4.1 | 4.1 | 4.3 | 4.1 | 6.2 | 5.9 | 6.2 | 6.6 | 6.5 | 7.1 | 6.6 | 6.7 | 6.5 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.4 | 1.9 | 1.3 | 1.6 | 1.4 | 1.7 | 1.3 | 1.3 | 1.5 | 1.1 | 1.2 | 0.9 | 1.1 | 1.5 | 1.8 | 1.8 | 1.5 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.13 | -0.11 | -0.28 | -0.16 | -0.23 | -0.23 | -0.19 | -0.23 | 0.36 | 0.28 | 0.36 | 0.43 | 0.41 | 0.56 | 0.44 | 0.46 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.7 | 0.7 | 0 | 0 | 0 |
Bud | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | -1.0 | -0.7 | -1.0 | -1.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -2.9 | -4.5 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | 0.5 | 0.4 | -0.2 | 0.4 | 0.1 | -0.4 | -0.6 | -0.3 | 0.9 | -1.8 | -1.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 1.6 | 5.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.8 | -6.0 | -9.5 | -5.5 | -6.2 | -5.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.7 | 0.5 | -4.6 | -2.0 | -2.5 |
SpindlePole | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.2 | -0.2 | -0.9 | 1.8 | 0.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2843 | 0.9407 | 0.6103 | 0.7462 | 0.5273 | 0.7679 | 2.8389 | 2.7053 | 2.7104 | 2.827 | 2.7075 | 3.047 | 1.6364 | 1.4475 | 1.4905 | 1.5614 | 1.2305 | 1.4791 |
Actin | 0.048 | 0.0131 | 0.014 | 0.0384 | 0.0034 | 0.0064 | 0.004 | 0.0338 | 0.0256 | 0.0044 | 0.0135 | 0.0056 | 0.0061 | 0.0229 | 0.0125 | 0.0003 | 0.0043 | 0.0019 |
Bud | 0.0002 | 0.0013 | 0.0004 | 0.0001 | 0.0008 | 0.0003 | 0 | 0.0006 | 0.0021 | 0 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0011 | 0.0065 | 0.0011 | 0.0014 | 0.0012 | 0.0001 | 0.0004 | 0.0007 | 0.0002 | 0 | 0.0005 | 0.0007 | 0.0003 | 0.0025 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0002 | 0.0025 | 0.0005 | 0.0002 | 0.0032 | 0.0003 | 0 | 0.0013 | 0.0006 | 0 | 0 | 0 | 0 | 0.0005 | 0.0003 | 0 | 0 | 0 |
Bud Site | 0.0009 | 0.0161 | 0.0041 | 0.0039 | 0.0019 | 0.0003 | 0.0002 | 0.0068 | 0.001 | 0.0006 | 0 | 0.0001 | 0.0021 | 0.0046 | 0.0014 | 0 | 0 | 0.0001 |
Cell Periphery | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.001 | 0.0011 | 0.0004 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0008 | 0.0008 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0878 | 0.1091 | 0.0879 | 0.0658 | 0.06 | 0.0594 | 0.0562 | 0.0663 | 0.0416 | 0.0385 | 0.0457 | 0.0573 | 0.0441 | 0.0888 | 0.0373 | 0.0265 | 0.0305 | 0.0453 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Endosome | 0.0444 | 0.0246 | 0.0059 | 0.0113 | 0.0166 | 0.0052 | 0.0036 | 0.0155 | 0.0018 | 0.0028 | 0.0004 | 0.001 | 0.006 | 0.0096 | 0.0018 | 0.001 | 0.0011 | 0.0043 |
Golgi | 0.0231 | 0.0163 | 0.0121 | 0.0123 | 0.0242 | 0.0098 | 0.0079 | 0.0069 | 0.0057 | 0.0055 | 0.0029 | 0.0038 | 0.0111 | 0.0167 | 0.0055 | 0.0037 | 0.0037 | 0.0082 |
Lipid Particles | 0.1058 | 0.0417 | 0.0273 | 0.0278 | 0.0687 | 0.0668 | 0.0482 | 0.0476 | 0.0139 | 0.0459 | 0.0669 | 0.0427 | 0.0498 | 0.0339 | 0.0149 | 0.0213 | 0.0178 | 0.0467 |
Mitochondria | 0.0203 | 0.0142 | 0.0153 | 0.0088 | 0.0344 | 0.0113 | 0.0077 | 0.0017 | 0.0082 | 0.0036 | 0.0006 | 0.0018 | 0.0069 | 0.008 | 0.0046 | 0.0007 | 0.0008 | 0.0027 |
None | 0.0009 | 0.0012 | 0.0009 | 0.0046 | 0.0007 | 0.0008 | 0.0048 | 0.0009 | 0.0005 | 0.0001 | 0 | 0.0018 | 0.0001 | 0.003 | 0.0007 | 0 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0114 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0005 | 0.0004 | 0.0002 | 0.0008 | 0.0179 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0048 | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.6436 | 0.7461 | 0.8143 | 0.8146 | 0.7555 | 0.8307 | 0.8593 | 0.8024 | 0.894 | 0.8976 | 0.8695 | 0.8813 | 0.87 | 0.8055 | 0.9165 | 0.945 | 0.9409 | 0.8888 |
Punctate Nuclear | 0.0052 | 0.0065 | 0.0096 | 0.0076 | 0.0032 | 0.0037 | 0.0067 | 0.0127 | 0.0029 | 0.0005 | 0.0004 | 0.0039 | 0.0025 | 0.0048 | 0.0014 | 0.0014 | 0.0005 | 0.0013 |
Vacuole | 0.0024 | 0.0036 | 0.0002 | 0.0003 | 0.0018 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Vacuole Periphery | 0.0017 | 0.0006 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1718 | 5.7539 | 13.4188 | 15.6958 | 10.1335 | 8.271 | 18.492 | 17.6696 | 16.9827 | 18.2343 |
Translational Efficiency | 0.462 | 0.9852 | 0.5475 | 0.4142 | 0.5888 | 1.0594 | 0.4584 | 0.4629 | 0.5791 | 0.4261 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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