Standard name | |
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Human Ortholog | |
Description | Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | – | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | 3 | 0 | 15 | 5 | 8 | 4 | 5 | 0 | 0 | 2 | 4 |
Bud | 6 | 3 | – | 2 | 6 | 3 | 1 | 9 | 10 | 6 | 20 | 14 | – | 1 | 1 | 16 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 1 | 0 | – | 2 | 3 | 0 | 2 | 4 | 3 | 3 | 2 | 3 | – | 0 | 0 | 2 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 4 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 3 | 2 | 4 | 4 | 6 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | – | 0 | 3 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | – | 1 | 3 | 17 | 7 | 8 | 0 | 2 | 0 | 0 | 0 | 0 |
Cytoplasm | 55 | 67 | – | 28 | 58 | 27 | 60 | 45 | 24 | 37 | 24 | 33 | – | 77 | 124 | 47 | 32 | 53 | 13 | 17 | 1 | 2 | 8 | 21 |
Endoplasmic Reticulum | 6 | 8 | – | 4 | 3 | 1 | 5 | 6 | 7 | 0 | 2 | 5 | – | 5 | 0 | 14 | 8 | 12 | 4 | 2 | 0 | 1 | 1 | 0 |
Endosome | 4 | 17 | – | 1 | 20 | 8 | 22 | 17 | 2 | 5 | 3 | 10 | – | 3 | 3 | 36 | 39 | 19 | 30 | 26 | 1 | 0 | 6 | 2 |
Golgi | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 2 | 3 | 2 | 4 | 0 | 0 | 1 | 0 |
Mitochondria | 102 | 59 | – | 8 | 19 | 77 | 105 | 114 | 146 | 168 | 232 | 208 | – | 2 | 1 | 32 | 23 | 24 | 140 | 145 | 21 | 15 | 39 | 73 |
Nucleus | 0 | 2 | – | 0 | 3 | 4 | 8 | 9 | 5 | 9 | 9 | 5 | – | 1 | 0 | 6 | 2 | 7 | 0 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | – | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 1 | – | 0 | 3 | 4 | 6 | 6 | 3 | 4 | 4 | 9 | – | 1 | 0 | 12 | 15 | 0 | 2 | 3 | 0 | 0 | 0 | 1 |
SpindlePole | 8 | 5 | – | 1 | 19 | 10 | 41 | 51 | 13 | 37 | 25 | 31 | – | 5 | 1 | 50 | 41 | 31 | 7 | 6 | 0 | 0 | 2 | 3 |
Vac/Vac Membrane | 5 | 19 | – | 1 | 14 | 7 | 13 | 16 | 7 | 11 | 2 | 11 | – | 2 | 2 | 46 | 23 | 19 | 6 | 20 | 4 | 0 | 1 | 8 |
Unique Cell Count | 159 | 159 | 46 | 130 | 116 | 225 | 231 | 184 | 236 | 262 | 267 | 96 | 132 | 231 | 164 | 164 | 223 | 248 | 34 | 24 | 72 | 130 | ||
Labelled Cell Count | 191 | 182 | 49 | 153 | 141 | 269 | 282 | 226 | 288 | 331 | 332 | 101 | 135 | 296 | 210 | 191 | 223 | 248 | 34 | 24 | 72 | 130 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.6 | – | 5.4 | 5.8 | 4.4 | 4.6 | 4.9 | 3.8 | 4.1 | 3.6 | 4.2 | – | 5.4 | 5.0 | 5.9 | 5.9 | 5.4 | – | 4.7 | 5.2 |
Std Deviation (1e-4) | 1.0 | 1.0 | – | 1.7 | 1.7 | 1.3 | 1.4 | 1.4 | 1.1 | 1.0 | 1.1 | 1.4 | – | 1.4 | 1.0 | 1.2 | 1.3 | 1.1 | – | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3671 | 0.4865 | 0.4091 | 0.7486 | -0.418 | 0.3001 | -0.3148 | 0.0711 | 0.1058 | -0.0387 | -0.0509 | 0.2472 | -1.0004 | -0.6312 | -1.1569 | -0.555 | -0.745 | -0.8475 |
Actin | 0.0312 | 0.0056 | 0.0317 | 0.0169 | 0.016 | 0.0094 | 0.0262 | 0.0213 | 0.0179 | 0.0078 | 0.0048 | 0.0159 | 0.0269 | 0.0121 | 0.0274 | 0.0076 | 0.0318 | 0.0154 |
Bud | 0.0033 | 0.0042 | 0.0066 | 0.0079 | 0.0016 | 0.0013 | 0.001 | 0.0029 | 0.0024 | 0.0022 | 0.0032 | 0.0008 | 0.0016 | 0.0042 | 0.0028 | 0.0047 | 0.0027 | 0.0009 |
Bud Neck | 0.0034 | 0.002 | 0.0026 | 0.0149 | 0.0071 | 0.0059 | 0.0092 | 0.0031 | 0.0036 | 0.0027 | 0.0017 | 0.022 | 0.0021 | 0.002 | 0.0034 | 0.0094 | 0.0012 | 0.0068 |
Bud Periphery | 0.0059 | 0.0066 | 0.0119 | 0.0119 | 0.0049 | 0.0034 | 0.002 | 0.0064 | 0.0054 | 0.0101 | 0.0092 | 0.0022 | 0.0024 | 0.0056 | 0.0091 | 0.0109 | 0.0103 | 0.0023 |
Bud Site | 0.0104 | 0.0366 | 0.0154 | 0.0629 | 0.0028 | 0.0016 | 0.0123 | 0.0335 | 0.0133 | 0.0029 | 0.0083 | 0.0013 | 0.0045 | 0.0516 | 0.0118 | 0.0046 | 0.0044 | 0.003 |
Cell Periphery | 0.0016 | 0.0016 | 0.0027 | 0.0012 | 0.0013 | 0.0007 | 0.0018 | 0.0026 | 0.0025 | 0.0014 | 0.0012 | 0.0013 | 0.0015 | 0.0027 | 0.0027 | 0.001 | 0.0013 | 0.0008 |
Cytoplasm | 0.0475 | 0.1117 | 0.0655 | 0.0794 | 0.1206 | 0.0729 | 0.0897 | 0.1353 | 0.1081 | 0.0734 | 0.1058 | 0.1222 | 0.1104 | 0.1364 | 0.103 | 0.1159 | 0.1001 | 0.1329 |
Cytoplasmic Foci | 0.1133 | 0.1202 | 0.1201 | 0.1214 | 0.1078 | 0.1687 | 0.1454 | 0.161 | 0.1218 | 0.1618 | 0.1066 | 0.1322 | 0.1441 | 0.1429 | 0.1278 | 0.1379 | 0.1131 | 0.1619 |
Eisosomes | 0.0021 | 0.0013 | 0.0015 | 0.0011 | 0.0008 | 0.0006 | 0.0015 | 0.0011 | 0.0015 | 0.0004 | 0.0004 | 0.0006 | 0.004 | 0.0016 | 0.0023 | 0.0002 | 0.0009 | 0.0016 |
Endoplasmic Reticulum | 0.0067 | 0.0046 | 0.0083 | 0.0091 | 0.0063 | 0.0059 | 0.0173 | 0.0071 | 0.0053 | 0.0048 | 0.0043 | 0.0086 | 0.0087 | 0.0077 | 0.0059 | 0.0026 | 0.0049 | 0.0091 |
Endosome | 0.1551 | 0.1825 | 0.1371 | 0.1504 | 0.189 | 0.1193 | 0.113 | 0.1105 | 0.1028 | 0.1995 | 0.1446 | 0.1086 | 0.1538 | 0.1287 | 0.1496 | 0.2319 | 0.1282 | 0.1486 |
Golgi | 0.0482 | 0.0492 | 0.044 | 0.0267 | 0.05 | 0.039 | 0.029 | 0.0436 | 0.0255 | 0.0487 | 0.018 | 0.0423 | 0.0355 | 0.0434 | 0.05 | 0.0346 | 0.0415 | 0.0292 |
Lipid Particles | 0.0812 | 0.0309 | 0.0335 | 0.021 | 0.0362 | 0.0472 | 0.064 | 0.0118 | 0.0341 | 0.0207 | 0.0193 | 0.022 | 0.0539 | 0.0145 | 0.0216 | 0.0247 | 0.0335 | 0.0234 |
Mitochondria | 0.1863 | 0.1438 | 0.2062 | 0.27 | 0.2812 | 0.2548 | 0.1052 | 0.1674 | 0.2332 | 0.1678 | 0.2631 | 0.2763 | 0.1372 | 0.1104 | 0.2193 | 0.1891 | 0.1489 | 0.2567 |
None | 0.2079 | 0.2353 | 0.2245 | 0.0874 | 0.0754 | 0.1623 | 0.2816 | 0.2235 | 0.239 | 0.2001 | 0.1884 | 0.1825 | 0.2493 | 0.2921 | 0.2057 | 0.1586 | 0.2352 | 0.1311 |
Nuclear Periphery | 0.009 | 0.0045 | 0.0066 | 0.0154 | 0.004 | 0.0065 | 0.0086 | 0.0009 | 0.0019 | 0.0011 | 0.0024 | 0.0041 | 0.0051 | 0.0018 | 0.0027 | 0.001 | 0.0283 | 0.003 |
Nucleolus | 0.0028 | 0.0016 | 0.0021 | 0.0012 | 0.0015 | 0.0008 | 0.0059 | 0.0013 | 0.0014 | 0.0007 | 0.0023 | 0.0011 | 0.0039 | 0.0014 | 0.0019 | 0.0009 | 0.0029 | 0.0006 |
Nucleus | 0.0042 | 0.0032 | 0.0038 | 0.0062 | 0.0058 | 0.0028 | 0.0033 | 0.0015 | 0.0013 | 0.0013 | 0.0028 | 0.004 | 0.0049 | 0.002 | 0.0033 | 0.0013 | 0.0084 | 0.0012 |
Peroxisomes | 0.06 | 0.0336 | 0.0508 | 0.0582 | 0.0607 | 0.0815 | 0.064 | 0.0478 | 0.0608 | 0.0687 | 0.0396 | 0.0361 | 0.0202 | 0.016 | 0.025 | 0.0386 | 0.0656 | 0.0468 |
Punctate Nuclear | 0.0049 | 0.0029 | 0.005 | 0.0032 | 0.0022 | 0.0058 | 0.0044 | 0.0012 | 0.0018 | 0.0028 | 0.0046 | 0.0015 | 0.0112 | 0.0013 | 0.0052 | 0.0038 | 0.0202 | 0.0028 |
Vacuole | 0.0101 | 0.0138 | 0.0142 | 0.0208 | 0.0153 | 0.0061 | 0.0111 | 0.0129 | 0.0121 | 0.0134 | 0.0571 | 0.0092 | 0.0145 | 0.0179 | 0.0142 | 0.0139 | 0.0109 | 0.0155 |
Vacuole Periphery | 0.0047 | 0.0041 | 0.0059 | 0.0127 | 0.0095 | 0.0035 | 0.0034 | 0.0031 | 0.0042 | 0.0077 | 0.0125 | 0.0052 | 0.0045 | 0.0039 | 0.0053 | 0.0071 | 0.006 | 0.0065 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.6092 | 9.4787 | 10.1108 | 8.8712 | 5.1554 | 7.2857 | 11.5306 | 10.107 | 7.7026 | 10.3584 |
Translational Efficiency | 0.6936 | 0.7892 | 0.5025 | 0.4688 | 1.1115 | 0.908 | 0.558 | 0.5389 | 0.5735 | 0.6997 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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