Standard name | |
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Human Ortholog | |
Description | Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 3 |
Bud | 0 | 2 | 1 | 0 | 0 | 2 | 6 | – | 5 | 9 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 14 | 10 | 10 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 5 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 13 | 1 | 3 | 2 | 5 | – | 2 | 1 | 1 | 1 | 0 | 3 | 0 | 7 | 4 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 185 | 285 | 351 | 63 | 252 | 168 | 234 | – | 112 | 140 | 88 | 145 | 15 | 263 | 139 | 169 | 159 | 182 | 186 | 287 | 372 | 91 | 319 | 343 |
Endoplasmic Reticulum | 1 | 2 | 4 | 0 | 3 | 1 | 6 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 10 | 8 | 6 | 0 | 0 | 0 | 3 | 12 | 10 |
Endosome | 0 | 2 | 0 | 6 | 23 | 16 | 15 | – | 5 | 3 | 3 | 22 | 3 | 23 | 20 | 13 | 5 | 3 | 0 | 3 | 5 | 6 | 4 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 1 |
Mitochondria | 2 | 7 | 0 | 8 | 13 | 52 | 82 | – | 189 | 183 | 256 | 112 | 16 | 3 | 6 | 4 | 1 | 0 | 0 | 5 | 8 | 3 | 7 | 4 |
Nucleus | 4 | 16 | 43 | 196 | 133 | 112 | 165 | – | 237 | 198 | 214 | 173 | 305 | 73 | 163 | 42 | 18 | 12 | 1 | 39 | 55 | 45 | 14 | 11 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 4 | 2 | – | 20 | 14 | 26 | 23 | 40 | 2 | 13 | 0 | 1 | 1 | 0 | 0 | 6 | 7 | 4 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 13 | 6 | 4 |
Vac/Vac Membrane | 0 | 34 | 50 | 55 | 144 | 94 | 102 | – | 93 | 88 | 89 | 136 | 25 | 95 | 107 | 115 | 72 | 58 | 1 | 18 | 21 | 25 | 22 | 24 |
Unique Cell Count | 190 | 332 | 445 | 318 | 529 | 398 | 541 | 551 | 543 | 553 | 544 | 365 | 424 | 416 | 313 | 231 | 247 | 194 | 372 | 499 | 219 | 415 | 431 | |
Labelled Cell Count | 193 | 350 | 465 | 331 | 576 | 452 | 620 | 671 | 639 | 688 | 628 | 404 | 462 | 449 | 363 | 268 | 273 | 194 | 372 | 499 | 219 | 415 | 431 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.4 | 7.5 | 6.9 | 7.4 | 6.1 | 6.2 | – | 5.5 | 5.2 | 4.8 | 5.7 | 7.5 | 7.3 | 7.3 | 9.6 | 9.9 | 9.7 | 6.8 | 6.7 | 7.4 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.8 | 1.0 | 1.1 | 1.1 | 1.1 | – | 1.0 | 0.9 | 0.8 | 0.9 | 1.8 | 1.5 | 1.3 | 2.1 | 1.7 | 1.6 | 1.1 | 1.6 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.03 | -0.3 | -0.27 | – | -0.44 | -0.52 | -0.66 | -0.4 | -0.01 | -0.04 | -0.03 | 0.36 | 0.4 | 0.36 | -0.14 | -0.17 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.6 | -2.9 | -2.7 | -2.3 | – | -3.3 | -3.6 | 0 | -3.6 | -3.3 | -2.4 | -3.5 | -0.6 | -0.9 | 0.8 |
Cytoplasm | -16.1 | -10.0 | -10.9 | -11.3 | – | -18.4 | -16.6 | -19.9 | -16.3 | -21.3 | -5.5 | -13.5 | -7.3 | -2.9 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 |
Endosome | 0 | 4.5 | 4.3 | 3.5 | – | 0 | 0 | 0 | 4.3 | 0 | 5.0 | 4.7 | 4.3 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.3 | 7.9 | 8.6 | – | 13.7 | 13.6 | 0 | 10.2 | 4.5 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 15.3 | 6.3 | 6.9 | 8.0 | – | 11.6 | 9.8 | 10.4 | 8.4 | 21.1 | 3.3 | 10.1 | 1.6 | -0.8 | -2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 4.1 | 3.4 | 0 | 4.4 | 7.2 | 0 | 3.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.4 | 6.2 | 4.8 | 3.3 | – | 2.5 | 2.2 | 2.2 | 5.5 | -2.1 | 4.4 | 5.5 | 8.4 | 6.4 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0055 | 3.8106 | 3.5088 | 3.0571 | 3.1196 | 3.3475 | 3.7862 | 5.3628 | 4.6566 | 4.4095 | 4.3594 | 4.6863 | 4.2463 | 5.1124 | 4.7479 | 4.3088 | 3.6164 | 4.5026 |
Actin | 0.0503 | 0.0032 | 0.0034 | 0.0149 | 0.0212 | 0.001 | 0.0319 | 0.0021 | 0.0105 | 0.0001 | 0.0295 | 0.001 | 0.0067 | 0.0008 | 0.0009 | 0.0003 | 0.0005 | 0.0006 |
Bud | 0.0023 | 0.0039 | 0.001 | 0.0023 | 0.0017 | 0.0047 | 0.0015 | 0.0042 | 0.003 | 0.0004 | 0.0002 | 0.0016 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0039 |
Bud Neck | 0.0015 | 0.0015 | 0.0031 | 0.0006 | 0.0017 | 0.0105 | 0.0025 | 0.0016 | 0.0009 | 0.0008 | 0.0007 | 0.0079 | 0.0002 | 0.0002 | 0.0004 | 0.0022 | 0.0007 | 0.0073 |
Bud Periphery | 0.0029 | 0.0015 | 0.0012 | 0.0013 | 0.0013 | 0.004 | 0.001 | 0.0021 | 0.0028 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.0033 |
Bud Site | 0.0063 | 0.0379 | 0.0286 | 0.0071 | 0.0021 | 0.0021 | 0.021 | 0.0255 | 0.005 | 0.0008 | 0.0004 | 0.0008 | 0.0008 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0016 |
Cell Periphery | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 |
Cytoplasm | 0.2851 | 0.544 | 0.3733 | 0.3667 | 0.4021 | 0.4511 | 0.3717 | 0.4715 | 0.4016 | 0.4364 | 0.3595 | 0.3926 | 0.5081 | 0.9928 | 0.7029 | 0.545 | 0.9324 | 0.7575 |
Cytoplasmic Foci | 0.0529 | 0.02 | 0.0136 | 0.0691 | 0.0337 | 0.0024 | 0.0565 | 0.0035 | 0.0075 | 0.0008 | 0.0064 | 0.0063 | 0.024 | 0.0008 | 0.0011 | 0.0019 | 0.0011 | 0.0031 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0089 | 0.0035 | 0.0046 | 0.0031 | 0.0045 | 0.0025 | 0.0063 | 0.0021 | 0.004 | 0.0005 | 0.001 | 0.0028 | 0.0161 | 0.0007 | 0.0023 | 0.0015 | 0.0018 | 0.0142 |
Endosome | 0.0568 | 0.0071 | 0.0245 | 0.0534 | 0.0281 | 0.002 | 0.0336 | 0.0019 | 0.0183 | 0.0003 | 0.0023 | 0.0022 | 0.3252 | 0.0002 | 0.0003 | 0.0013 | 0.0004 | 0.0021 |
Golgi | 0.015 | 0.0011 | 0.0075 | 0.0249 | 0.022 | 0.0002 | 0.0111 | 0.0004 | 0.0019 | 0 | 0.0078 | 0.0002 | 0.093 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 |
Lipid Particles | 0.0198 | 0.0012 | 0.0089 | 0.0274 | 0.0025 | 0.0001 | 0.0168 | 0.0001 | 0.0022 | 0 | 0.0071 | 0.0001 | 0.0038 | 0 | 0 | 0 | 0 | 0.0001 |
Mitochondria | 0.0259 | 0.001 | 0.0063 | 0.0194 | 0.01 | 0.0007 | 0.0037 | 0.0005 | 0.0052 | 0.0003 | 0.0011 | 0.0127 | 0.0055 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
None | 0.015 | 0.0031 | 0.0041 | 0.002 | 0.0024 | 0.0011 | 0.0703 | 0.0068 | 0.0079 | 0.0006 | 0.0008 | 0.0139 | 0.002 | 0.0015 | 0.0034 | 0.0014 | 0.0034 | 0.0288 |
Nuclear Periphery | 0.0202 | 0.0073 | 0.0058 | 0.0046 | 0.0107 | 0.0076 | 0.0108 | 0.0082 | 0.006 | 0.0044 | 0.0065 | 0.0059 | 0.0043 | 0.0004 | 0.0047 | 0.0055 | 0.0044 | 0.0047 |
Nucleolus | 0.0121 | 0.0026 | 0.0039 | 0.0008 | 0.0011 | 0.0054 | 0.0027 | 0.0025 | 0.0024 | 0.0016 | 0.0034 | 0.0046 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0006 |
Nucleus | 0.3722 | 0.3391 | 0.4937 | 0.3539 | 0.3859 | 0.4899 | 0.3304 | 0.4257 | 0.5013 | 0.5509 | 0.5383 | 0.5378 | 0.0009 | 0.0015 | 0.2809 | 0.4336 | 0.0535 | 0.1642 |
Peroxisomes | 0.029 | 0.0057 | 0.0035 | 0.0438 | 0.0614 | 0.0001 | 0.0076 | 0.0002 | 0.0029 | 0 | 0.0308 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0124 | 0.0073 | 0.0031 | 0.002 | 0.0053 | 0.0094 | 0.0145 | 0.0372 | 0.0069 | 0.0014 | 0.0031 | 0.0052 | 0.0004 | 0.0001 | 0.0019 | 0.0038 | 0.0002 | 0.0022 |
Vacuole | 0.0071 | 0.0075 | 0.0084 | 0.0019 | 0.0016 | 0.0044 | 0.0046 | 0.0029 | 0.0069 | 0.0006 | 0.0006 | 0.003 | 0.0038 | 0.0001 | 0.0002 | 0.0015 | 0.0009 | 0.0046 |
Vacuole Periphery | 0.0034 | 0.0009 | 0.0009 | 0.0007 | 0.0006 | 0.0006 | 0.0008 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0.0004 | 0.0039 | 0 | 0 | 0.0002 | 0.0002 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0422 | 14.9239 | 10.7281 | 21.7011 | 19.616 | 10.9497 | 25.6994 | 25.7793 | 17.776 | 27.9583 |
Translational Efficiency | 1.4883 | 1.827 | 1.7955 | 0.8763 | 1.2786 | 3.1763 | 1.1078 | 0.957 | 1.6233 | 1.1329 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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