Standard name | |
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Human Ortholog | |
Description | Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within N-terminus is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress; human homolog CCS can complement yeast ccs1 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 7 | 1 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 2 | 1 | 2 | 14 | 3 | 7 | 11 | 10 | 15 | 12 | 2 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 363 | 191 | 209 | 209 | 241 | 449 | 244 | 389 | 305 | 272 | 314 | 331 | 158 | 275 | 286 | 109 | 100 | 94 | 355 | 176 | 205 | 132 | 260 | 235 |
Endoplasmic Reticulum | 4 | 1 | 1 | 1 | 6 | 17 | 3 | 19 | 15 | 18 | 18 | 9 | 0 | 2 | 2 | 2 | 4 | 3 | 0 | 0 | 2 | 1 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 0 | 10 | 4 | 11 | 8 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleus | 2 | 0 | 2 | 1 | 0 | 17 | 9 | 5 | 5 | 10 | 18 | 12 | 0 | 0 | 1 | 7 | 0 | 2 | 4 | 4 | 1 | 2 | 1 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 2 | 3 | 1 | 19 | 11 | 42 | 95 | 31 | 38 | 31 | 39 | 0 | 5 | 2 | 0 | 0 | 2 | 1 | 5 | 0 | 1 | 3 | 2 |
Unique Cell Count | 366 | 192 | 211 | 213 | 262 | 496 | 297 | 512 | 368 | 336 | 397 | 403 | 158 | 281 | 292 | 112 | 103 | 94 | 365 | 188 | 217 | 143 | 272 | 252 |
Labelled Cell Count | 374 | 194 | 217 | 213 | 272 | 512 | 311 | 524 | 385 | 354 | 416 | 418 | 160 | 286 | 294 | 118 | 109 | 104 | 365 | 188 | 217 | 143 | 272 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.0 | 21.2 | 22.1 | 22.0 | 16.6 | 18.9 | 11.6 | 17.5 | 15.1 | 15.5 | 15.1 | 14.8 | 25.8 | 22.8 | 23.1 | 24.5 | 28.2 | 30.7 | 21.7 | 22.4 | 23.7 |
Std Deviation (1e-4) | 4.3 | 4.3 | 4.5 | 4.6 | 3.7 | 3.6 | 3.0 | 3.2 | 2.8 | 2.7 | 2.7 | 2.8 | 5.4 | 4.6 | 4.8 | 6.0 | 9.3 | 9.4 | 5.4 | 4.8 | 5.4 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.41 | -0.23 | -0.93 | -0.34 | -0.55 | -0.51 | -0.55 | -0.58 | 0.22 | 0.04 | 0.06 | 0.15 | 0.35 | 0.47 | -0.03 | 0.02 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -3.6 | -4.1 | -6.0 | -7.5 | -5.9 | -6.3 | -6.8 | -6.1 | 1.2 | -1.0 | -1.0 | -1.2 | -1.3 | 0.9 |
Endoplasmic Reticulum | 0 | 0 | 2.3 | 0 | 2.4 | 2.5 | 3.0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.0 | 0 | 5.0 | 6.1 | 3.4 | 4.3 | 3.3 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.3669 | 26.5495 | 23.3326 | 21.2142 | 19.0515 | 22.6407 | 22.6064 | 26.7667 | 24.0758 | 26.8878 | 23.8241 | 25.6976 | 22.0228 | 26.9678 | 23.9973 | 21.5342 | 22.48 | 24.5766 |
Actin | 0.0094 | 0.0001 | 0.0001 | 0.0001 | 0.0363 | 0.0004 | 0.013 | 0.0006 | 0.0011 | 0 | 0.0034 | 0.0001 | 0.0183 | 0.0001 | 0.0024 | 0.0013 | 0.0016 | 0.0001 |
Bud | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0009 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0003 |
Bud Neck | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.001 | 0.0006 | 0.0005 | 0.0007 | 0.0019 | 0.0001 | 0.0003 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0.0005 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0003 |
Bud Site | 0.0038 | 0 | 0 | 0 | 0.0009 | 0.0001 | 0.0029 | 0.0045 | 0.0001 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0005 | 0.0007 | 0.0004 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.8612 | 0.9801 | 0.9719 | 0.9716 | 0.7822 | 0.9306 | 0.8645 | 0.9799 | 0.9598 | 0.992 | 0.9435 | 0.9692 | 0.9088 | 0.9918 | 0.9461 | 0.9009 | 0.8753 | 0.9671 |
Cytoplasmic Foci | 0.0182 | 0.0001 | 0.0002 | 0.0003 | 0.0063 | 0.0007 | 0.0206 | 0.0028 | 0.0076 | 0.0002 | 0.0052 | 0.0018 | 0.0052 | 0.0002 | 0.0062 | 0.0025 | 0.0153 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0025 | 0.0002 | 0.0004 | 0.0009 | 0.0036 | 0.0015 | 0.0038 | 0.0006 | 0.0009 | 0.0001 | 0.0026 | 0.0009 | 0.0025 | 0.0002 | 0.0014 | 0.0009 | 0.0037 | 0.0008 |
Endosome | 0.0061 | 0 | 0.0006 | 0.0003 | 0.0161 | 0.0008 | 0.0199 | 0.0003 | 0.0009 | 0 | 0.0095 | 0.0024 | 0.0038 | 0 | 0.0039 | 0.0086 | 0.02 | 0.0004 |
Golgi | 0.0022 | 0 | 0 | 0 | 0.008 | 0 | 0.0051 | 0.0001 | 0.0002 | 0 | 0.0017 | 0.0001 | 0.0018 | 0 | 0.001 | 0.0022 | 0.005 | 0 |
Lipid Particles | 0.006 | 0 | 0 | 0 | 0.0344 | 0 | 0.0063 | 0.0002 | 0.0023 | 0 | 0.0036 | 0.0007 | 0.0017 | 0 | 0.0027 | 0.004 | 0.0381 | 0 |
Mitochondria | 0.0102 | 0 | 0.0009 | 0.0001 | 0.0125 | 0.0005 | 0.0021 | 0.0002 | 0.0011 | 0 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0045 | 0.0316 | 0.0115 | 0.0044 |
None | 0.0037 | 0.0001 | 0.0002 | 0.0003 | 0.0042 | 0.0005 | 0.0054 | 0.001 | 0.0018 | 0.0002 | 0.0036 | 0.0007 | 0.0025 | 0.0003 | 0.0018 | 0.0005 | 0.0028 | 0.0003 |
Nuclear Periphery | 0.0115 | 0.0024 | 0.004 | 0.0033 | 0.0278 | 0.0085 | 0.0086 | 0.0006 | 0.0014 | 0.0005 | 0.0104 | 0.0029 | 0.0055 | 0.0007 | 0.0026 | 0.0023 | 0.0022 | 0.0019 |
Nucleolus | 0.0006 | 0 | 0.0001 | 0 | 0.0047 | 0 | 0.001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0008 | 0.0002 | 0.0008 | 0 |
Nucleus | 0.049 | 0.0167 | 0.0183 | 0.0219 | 0.0244 | 0.0537 | 0.0305 | 0.0061 | 0.0159 | 0.0066 | 0.0106 | 0.0186 | 0.0376 | 0.0065 | 0.0182 | 0.0381 | 0.0144 | 0.0224 |
Peroxisomes | 0.0067 | 0 | 0 | 0 | 0.0108 | 0 | 0.0049 | 0.0001 | 0.001 | 0 | 0.0007 | 0 | 0.0012 | 0 | 0.0008 | 0.001 | 0.0028 | 0 |
Punctate Nuclear | 0.0032 | 0.0001 | 0.0001 | 0.0002 | 0.0205 | 0.0004 | 0.008 | 0.0012 | 0.0006 | 0.0001 | 0.0025 | 0.0011 | 0.0073 | 0 | 0.0054 | 0.0002 | 0.0011 | 0.0002 |
Vacuole | 0.002 | 0.0001 | 0.0016 | 0.0006 | 0.0016 | 0.0011 | 0.0016 | 0.0004 | 0.0013 | 0 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0005 | 0.0026 | 0.0024 | 0.0005 |
Vacuole Periphery | 0.0017 | 0 | 0.001 | 0.0001 | 0.0024 | 0.0004 | 0.0006 | 0 | 0.0004 | 0 | 0.0007 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0013 | 0.0012 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.4792 | 144.6685 | 139.5554 | 133.5627 | 117.3102 | 114.6905 | 139.9578 | 134.6253 | 152.4931 | 112.9448 |
Translational Efficiency | 2.366 | 1.9417 | 2.2063 | 2.807 | 2.6265 | 1.9021 | 1.8224 | 2.6972 | 1.8964 | 2.1265 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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