Standard name
Human Ortholog
Description Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.39 0.49 0.64 0.69 0.67 0.58 0.4 0.4 0.3 0.31 0.27 0.26 0.74 0.71 0.67 0.53 0.54 0.51 0.38 0.28 0.28 0.55 0.57 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.11 0.05 0.08 0.12 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0 0 0.05 0 0.23 0.42 0.39 0.65 0.55 0.65 0.52 0 0 0 0.05 0 0 0.1 0 0.14 0.05 0.07 0.05
Nucleus 0.11 0.21 0.2 0.16 0.16 0.18 0.31 0.34 0.33 0.32 0.31 0.36 0.15 0.1 0.11 0.16 0.08 0.11 0.15 0.24 0.18 0.11 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0.08 0 0 0 0.08 0.07 0.06 0 0 0
Vac/Vac Membrane 0.22 0.4 0.1 0.06 0.09 0.08 0.09 0.05 0 0.06 0.08 0.09 0 0 0.07 0.32 0.4 0.32 0.15 0.17 0.18 0.18 0.14 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 3 8
Bud 1 0 1 0 1 0 1 2 2 3 6 2 0 0 0 0 1 1 2 3 1 3 10 7
Bud Neck 0 0 1 1 5 0 0 1 1 1 0 0 1 12 13 0 0 1 0 0 0 1 4 5
Bud Site 0 0 0 0 0 1 3 3 3 4 4 5 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 7 34 38 100 142 140 127 88 66 64 63 42 129 156 238 72 97 61 22 31 38 115 170 254
Endoplasmic Reticulum 0 0 0 1 0 1 0 0 0 0 0 0 2 1 1 3 9 8 0 0 1 1 1 3
Endosome 0 3 0 2 10 4 0 0 0 0 0 0 2 4 39 7 15 14 2 5 1 2 3 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 0 2 2 1
Mitochondria 6 0 0 7 8 56 132 87 145 114 151 86 1 1 0 7 7 3 5 4 19 10 20 19
Nucleus 2 15 12 23 34 44 98 75 73 67 71 59 27 23 39 22 14 13 8 26 23 23 16 8
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 1 1 0 0 1 0 0 0 0 0 0 0 1 2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 6 7 5 5 8 1 5 2 3 17 26 29 1 0 0 4 7 8 4 11 10
Vac/Vac Membrane 4 28 6 9 19 19 28 10 6 12 18 15 5 7 26 44 72 38 9 19 23 37 40 36
Unique Cell Count 18 70 59 145 211 240 314 221 222 209 232 164 175 221 356 136 178 119 59 110 134 211 296 379
Labelled Cell Count 20 82 59 150 226 272 394 275 298 270 315 213 184 231 385 157 216 139 59 110 134 211 296 379


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 5.0 4.5 4.2 5.0 3.9 3.7 4.0 3.3 3.6 3.5 3.8 5.0 5.2 5.1 6.4 6.5 5.9 5.0 5.0 5.3
Std Deviation (1e-4) 0.5 1.6 0.9 0.9 2.2 1.4 1.6 1.5 0.8 1.1 1.3 1.3 1.2 1.4 1.3 1.4 2.5 1.8 1.6 1.7 1.8
Intensity Change (Log2) – – – -0.09 0.16 -0.2 -0.26 -0.15 -0.42 -0.32 -0.37 -0.23 0.15 0.22 0.2 0.52 0.55 0.41 0.17 0.16 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.4 -0.9 -3.4 -3.4 -4.9 -4.7 -5.4 -5.3 1.4 0.9 0.4 -1.5 -1.3 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.8 -0.8 -0.4 1.7 2.0 1.9 1.7 1.6 2.2 -0.9 -2.0 -2.0 -0.7 -2.7 -1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2.3 0 0 0 0
Vacuole 0 -0.3 0 -0.3 0 0 0 0 -0.2 0 0 0 3.3 4.3 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3069 -0.7918 -0.9664 -0.7372 1.5868 -0.6956 0.2165 -0.4296 -0.35 -0.0338 -0.4367 -0.4089 -1.4414 -1.3649 -1.5291 -1.0392 -2.0606 -1.4222
Actin 0.0332 0.0004 0.0017 0.0621 0.0289 0.0114 0.0494 0.0001 0.0137 0.0315 0.0043 0.0077 0.0301 0.0002 0.0021 0.1394 0.0001 0.0005
Bud 0.0004 0.0002 0.0019 0.0005 0.0006 0.0046 0.0012 0.0001 0.0009 0.0005 0.0002 0.0003 0.0003 0.0001 0.0001 0.0009 0.0019 0.0001
Bud Neck 0.0045 0.001 0.0052 0.007 0.0022 0.0021 0.0072 0.0004 0.0021 0.0025 0.0005 0.005 0.0016 0.0009 0.0008 0.0045 0.0019 0.0037
Bud Periphery 0.0005 0.0002 0.0006 0.001 0.0013 0.0011 0.0029 0.0002 0.0011 0.0009 0.0003 0.0008 0.0005 0.0001 0.0001 0.0013 0.0008 0.0001
Bud Site 0.0025 0.0011 0.0068 0.0046 0.0023 0.0002 0.0116 0.0009 0.0024 0.0019 0.0005 0.0003 0.0009 0.001 0.0008 0.0081 0.0012 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0.0003 0.0002 0.0003 0.0006 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001
Cytoplasm 0.0707 0.0717 0.0817 0.0482 0.0693 0.0996 0.0686 0.0966 0.0661 0.0258 0.0562 0.073 0.1329 0.1338 0.0802 0.0888 0.1713 0.1966
Cytoplasmic Foci 0.0266 0.0094 0.0053 0.0054 0.0183 0.0162 0.0415 0.005 0.0091 0.0215 0.0233 0.0039 0.0106 0.0163 0.0163 0.0085 0.0167 0.0196
Eisosomes 0.0004 0.0001 0.0001 0.0006 0.0006 0.0002 0.0006 0.0001 0.0006 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0015 0 0.0001
Endoplasmic Reticulum 0.0062 0.0022 0.0032 0.0024 0.0168 0.0026 0.0052 0.0023 0.0017 0.003 0.0006 0.0017 0.0068 0.0036 0.0039 0.0025 0.0022 0.005
Endosome 0.0203 0.0038 0.0038 0.0093 0.0694 0.0043 0.0397 0.0019 0.0124 0.0349 0.0142 0.0068 0.0155 0.0015 0.007 0.0133 0.0022 0.0031
Golgi 0.0066 0.0005 0.0003 0.0065 0.0109 0.001 0.0141 0.0001 0.005 0.0193 0.0057 0.0025 0.004 0.0001 0.0008 0.0098 0.0001 0.0001
Lipid Particles 0.017 0.002 0.0009 0.0056 0.0478 0.0018 0.0428 0.0004 0.005 0.0281 0.0255 0.0045 0.0055 0.0005 0.0065 0.0113 0.0007 0.0005
Mitochondria 0.0029 0.0023 0.0015 0.0019 0.009 0.0012 0.0192 0.0005 0.0078 0.0098 0.0017 0.0044 0.0024 0.0004 0.0005 0.0138 0.0004 0.0007
None 0.4137 0.4884 0.3899 0.6022 0.2908 0.4291 0.2484 0.4509 0.3188 0.5841 0.2448 0.309 0.2485 0.2995 0.2159 0.3082 0.3794 0.203
Nuclear Periphery 0.055 0.0131 0.0316 0.0112 0.1784 0.019 0.0347 0.0146 0.023 0.0209 0.0079 0.0143 0.0458 0.0189 0.0173 0.0157 0.0096 0.0086
Nucleolus 0.0091 0.0132 0.0097 0.0031 0.0078 0.0054 0.0144 0.0117 0.0212 0.0087 0.0035 0.0115 0.0074 0.0122 0.0105 0.0027 0.0097 0.0138
Nucleus 0.2423 0.334 0.3546 0.2003 0.0583 0.3191 0.3065 0.3432 0.4518 0.1149 0.3655 0.4763 0.428 0.4499 0.5378 0.3474 0.3273 0.5122
Peroxisomes 0.0068 0.0003 0.0087 0.002 0.0471 0.0009 0.0348 0.0018 0.0105 0.0305 0.1133 0.003 0.002 0.0002 0.001 0.0067 0.0002 0.0002
Punctate Nuclear 0.0767 0.0541 0.0831 0.0244 0.1292 0.0783 0.0482 0.0672 0.0422 0.0572 0.1315 0.0732 0.0509 0.0587 0.0965 0.0129 0.0726 0.0298
Vacuole 0.0028 0.0016 0.007 0.0009 0.0052 0.0011 0.0062 0.0017 0.0034 0.002 0.0004 0.0009 0.0045 0.0016 0.0015 0.0014 0.0011 0.0019
Vacuole Periphery 0.0018 0.0005 0.0022 0.0005 0.0058 0.0005 0.0021 0.0002 0.0007 0.0015 0.0002 0.0007 0.0017 0.0004 0.0004 0.001 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7227 9.3054 3.8361 9.1961 16.4955 13.8716 6.2725 5.6447 11.97 15.4343
Translational Efficiency 2.542 1.4025 2.1601 1.4954 1.4503 3.0233 1.6335 1.7074 1.1621 1.6378

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (22%), cytoplasm (12%), mixed (48%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.8e-06)
Subcompartmental Group N/A

Csm3

Csm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Csm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available