Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.99 0.98 0.99 0.98 0.94 – 0.81 0.84 0.71 0.86 0.99 0.99 1.0 0.98 0.96 0.97 0.95 0.92 0.89 0.9 0.87 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.12 – 0.32 0.23 0.44 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 2
Bud 0 3 0 0 0 1 2 – 3 3 2 4 1 0 2 0 0 0 0 0 1 1 3 11
Bud Neck 0 0 2 0 4 0 3 – 2 1 0 1 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 3 0 2 1 2 – 7 5 2 4 0 0 0 4 3 7 0 0 0 0 0 0
Cytoplasm 200 285 238 126 274 250 365 – 240 281 177 236 284 382 356 300 256 193 190 272 220 182 254 314
Endoplasmic Reticulum 0 1 0 0 0 1 3 – 2 3 3 0 0 0 0 12 15 8 0 0 2 0 3 4
Endosome 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 1 1 1 5 2 0 1 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0 0 0 2 1 1
Mitochondria 2 6 1 5 1 13 45 – 94 77 110 69 2 2 1 8 4 0 2 5 6 6 10 10
Nucleus 0 0 2 0 0 1 6 – 3 4 5 1 0 0 0 1 0 2 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1
SpindlePole 0 0 0 0 0 0 0 – 0 2 0 3 1 0 0 0 1 0 0 0 2 1 1 1
Vac/Vac Membrane 0 2 1 0 0 0 3 – 3 4 3 1 0 0 0 6 7 3 0 5 2 2 5 6
Unique Cell Count 200 292 240 128 277 254 387 295 335 251 275 286 384 357 307 267 198 200 295 248 202 292 364
Labelled Cell Count 204 300 247 131 281 267 431 354 381 303 319 288 385 360 332 287 215 200 295 248 202 292 364


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.3 5.0 4.4 5.3 4.2 4.5 – 3.9 3.9 3.5 4.1 4.7 4.7 4.9 6.3 6.5 6.3 4.4 4.6 4.8
Std Deviation (1e-4) 0.4 0.7 1.1 1.0 1.3 0.8 1.1 – 0.7 0.8 0.8 0.7 1.1 1.1 1.3 0.8 1.3 1.0 1.2 1.3 1.5
Intensity Change (Log2) – – – -0.19 0.08 -0.25 -0.18 – -0.39 -0.39 -0.52 -0.29 -0.11 -0.09 -0.05 0.31 0.37 0.32 -0.2 -0.14 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6537 2.3765 2.3131 2.7929 2.7376 2.4377 -0.6495 -0.1097 -0.0846 -0.0386 -0.3674 -0.2699 -0.7994 -0.0852 -0.1443 0.1073 -0.4755 -0.3489
Actin 0.0224 0.001 0.0114 0.0253 0.0027 0.0068 0.0321 0.0016 0.0234 0.0021 0.0014 0.0039 0.0032 0.0001 0.0188 0.0011 0.0004 0.0003
Bud 0.0017 0.0015 0.0028 0.001 0.0022 0.0006 0.0065 0.0058 0.0035 0.0016 0.0002 0.0007 0.0007 0 0.0009 0.0004 0 0
Bud Neck 0.0133 0.0007 0.0005 0.0049 0.0438 0.0073 0.0064 0.0004 0.0006 0.0014 0.0003 0.0005 0.0002 0 0.0087 0.0005 0.0139 0.0007
Bud Periphery 0.0044 0.0025 0.0062 0.0019 0.0044 0.0004 0.0097 0.0122 0.0059 0.0025 0.0003 0.0014 0.0006 0 0.0025 0.0004 0 0
Bud Site 0.014 0.0294 0.0033 0.0045 0.0025 0.001 0.0084 0.0148 0.0089 0.0009 0.0005 0.0009 0.0008 0.0001 0.0033 0.0035 0 0
Cell Periphery 0.0012 0.0007 0.0002 0.001 0.0005 0.0001 0.0004 0.0007 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0
Cytoplasm 0.0624 0.124 0.0914 0.0684 0.1669 0.2011 0.2086 0.3616 0.3104 0.3557 0.2701 0.4156 0.239 0.4294 0.4132 0.4762 0.364 0.4746
Cytoplasmic Foci 0.0191 0.006 0.009 0.0072 0.036 0.0093 0.0197 0.0098 0.0152 0.0406 0.0269 0.0116 0.034 0.0047 0.0174 0.0078 0.0038 0.0045
Eisosomes 0.0006 0.0003 0.0002 0.0003 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0 0.0001 0 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0028 0.0008 0.0074 0.0007 0.0079 0.0012 0.0051 0.0015 0.002 0.0012 0.0011 0.0013 0.0023 0.0007 0.0011 0.0014 0.0039 0.0043
Endosome 0.0114 0.0009 0.0125 0.0069 0.0409 0.0036 0.0194 0.0017 0.0094 0.0264 0.0256 0.0054 0.0138 0.0015 0.0047 0.0024 0.0016 0.0015
Golgi 0.0094 0.0003 0.007 0.0148 0.0055 0.0023 0.007 0.0003 0.0077 0.0032 0.0085 0.0024 0.002 0.0001 0.0025 0.0003 0.0001 0.0001
Lipid Particles 0.0137 0.0004 0.0053 0.0156 0.0027 0.0011 0.0056 0.0002 0.0025 0.0039 0.007 0.003 0.0045 0.0001 0.005 0.0002 0.0001 0.0001
Mitochondria 0.0107 0.0006 0.0166 0.0239 0.0608 0.0027 0.0056 0.0009 0.0209 0.001 0.0143 0.0042 0.0009 0.0001 0.0016 0.0002 0.0006 0.0008
None 0.7651 0.8257 0.8048 0.7976 0.5927 0.7497 0.637 0.5759 0.5795 0.5463 0.6073 0.5386 0.6699 0.5616 0.5035 0.5012 0.5594 0.4982
Nuclear Periphery 0.0033 0.0004 0.0027 0.0004 0.0091 0.0056 0.0053 0.0006 0.0009 0.0012 0.0071 0.0013 0.0051 0.0002 0.0009 0.0006 0.0085 0.002
Nucleolus 0.0021 0.0002 0.0006 0.0043 0.0036 0.0001 0.0008 0.0004 0.0003 0.0002 0.0004 0.0003 0.0006 0 0.0028 0.0001 0.0005 0.0002
Nucleus 0.0171 0.0009 0.0013 0.0007 0.0057 0.0022 0.0031 0.0034 0.0013 0.0019 0.0037 0.0028 0.0049 0.0005 0.0025 0.0012 0.0415 0.0106
Peroxisomes 0.0131 0.0007 0.0124 0.0128 0.0029 0.0029 0.0071 0.0048 0.0034 0.0054 0.0142 0.0018 0.0033 0 0.0057 0.0001 0 0.0001
Punctate Nuclear 0.008 0.0021 0.0021 0.0004 0.0023 0.0011 0.0085 0.0015 0.0015 0.0029 0.0088 0.0032 0.0058 0.0002 0.0033 0.001 0.0007 0.0013
Vacuole 0.0032 0.0009 0.0016 0.0063 0.0052 0.0005 0.0021 0.0016 0.0018 0.0011 0.0014 0.0007 0.0058 0.0005 0.001 0.0012 0.0007 0.0004
Vacuole Periphery 0.0008 0.0001 0.0007 0.001 0.0015 0.0003 0.0012 0.0002 0.0006 0.0003 0.0007 0.0002 0.0023 0.0001 0.0003 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.4396 87.0811 78.8097 57.0495 59.9039 52.774 56.1612 45.125 37.0355 55.1776
Translational Efficiency 2.2129 2.2622 1.706 1.899 1.918 2.0204 1.8029 1.5712 1.5808 1.6758

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Erb1

Erb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available