Standard name
Human Ortholog
Description Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.09 0 0 0 0 0 0 – 0 0 0 0.11 0.12 0 0 0.17 0.06 0 0.05 0 0 0 0
Bud 0 0 0 0.06 0 0 0.07 0.08 – 0.07 0.07 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.09 0.09 0.11 0.15 0.11 0.1 0.09 – 0.07 0.05 0.05 0.11 0.12 0.14 0.26 0.36 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.35 0 0.43 0.35 0.36 0 0 0.09 – 0 0 0 0.11 0.12 0.35 0.36 0.22 0.33 0.33 0.31 0.36 0.26 0.31 0.29
Golgi 0.06 0.06 0.05 0.06 0.05 0 0 0 – 0 0 0 0 0.15 0.22 0 0.05 0.08 0.3 0.22 0.28 0.25 0.29 0.32
Mitochondria 0.49 0.83 0.33 0.55 0.44 0.86 0.82 0.79 – 0.9 0.88 0.91 0.72 0.58 0.28 0.12 0.08 0.16 0.15 0.24 0.08 0.18 0.18 0.17
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0.17 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0.18 0.08 0.05 0 0.08 0 – 0 0 0 0 0.08 0 0.05 0 0 0.11 0.1 0.19 0.19 0.09 0.08
SpindlePole 0.1 0 0.2 0.07 0.07 0 0.07 0.1 – 0 0 0 0.11 0 0.07 0.23 0.14 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0.07 0.07 0 0 0 – 0 0.05 0 0 0 0.06 0.14 0.12 0.08 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 13 6 0 1 1 0 1 – 1 5 0 2 3 1 4 10 3 10 8 6 1 4 5
Bud 9 3 10 12 9 9 24 23 – 20 19 25 0 0 0 4 0 3 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 2 0 0 2 3 1 – 4 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 – 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 33 12 23 22 33 32 32 26 – 22 14 17 2 3 13 62 21 17 3 1 3 0 1 0
Endoplasmic Reticulum 10 9 3 0 1 3 3 1 – 5 1 5 0 1 4 16 2 2 0 0 2 0 2 0
Endosome 128 5 109 72 76 10 7 26 – 4 4 10 2 3 33 87 13 17 152 47 115 14 42 44
Golgi 23 8 13 12 10 1 0 0 – 1 3 0 0 4 21 4 3 4 138 34 91 13 40 48
Mitochondria 178 116 83 113 95 240 270 222 – 268 227 306 13 15 26 30 5 8 69 36 25 10 25 25
Nucleus 2 0 0 0 1 3 1 2 – 0 2 0 1 0 0 0 2 3 0 0 0 0 0 0
Nuclear Periphery 0 3 1 0 0 0 0 0 – 2 2 1 0 0 0 1 1 0 0 0 1 0 0 0
Nucleolus 2 0 0 1 3 6 6 7 – 6 4 2 3 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 29 0 45 16 10 6 26 9 – 7 6 2 0 2 4 11 0 0 50 15 62 10 12 12
SpindlePole 36 6 51 14 14 11 24 28 – 11 11 10 2 0 7 56 8 10 14 3 3 0 1 1
Vac/Vac Membrane 31 5 7 15 16 11 12 6 – 7 12 15 0 1 6 35 7 4 14 4 3 0 5 7
Unique Cell Count 361 139 252 204 214 279 328 280 297 257 336 18 26 94 243 59 51 464 155 322 55 139 151
Labelled Cell Count 490 180 353 277 269 335 408 352 359 316 398 25 32 117 311 72 71 464 155 322 55 139 151


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.3 5.9 5.8 5.6 4.9 4.6 4.9 – 4.4 4.7 4.7 6.2 6.7 7.3 5.5 6.2 6.6 5.6 7.4 8.0
Std Deviation (1e-4) 0.8 0.7 1.6 1.4 1.6 1.5 1.3 1.5 – 1.0 1.0 1.0 1.0 1.0 1.4 1.1 1.1 1.1 0.7 2.0 2.5
Intensity Change (Log2) – – – -0.03 -0.06 -0.26 -0.37 -0.26 – -0.41 -0.32 -0.32 0.09 0.19 0.31 -0.1 0.07 0.15 -0.06 0.33 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0.9 0.1 -0.5 0 2.0 – 0 0 0 0 0 0 -1.6 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 2.1 0.9 0.3 0.1 – -0.7 -1.6 -1.9 0 0 1.3 4.8 5.3 4.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 3.1 0 0
Endosome -1.7 -1.7 -10.9 0 -9.0 – 0 0 0 -2.7 -3.1 -1.4 -1.7 -3.0 -1.3
Golgi 0.3 -0.2 -3.4 0 -3.8 – 0 0 0 0 0 4.8 -2.1 0 0
Mitochondria 4.8 2.5 12.5 0 10.8 – 0 0 0 3.4 2.5 -0.9 -5.5 -3.8 -2.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -3.1 -4.4 -6.1 0 -5.6 – 0 0 0 0 0 -3.2 -4.7 -3.5 -3.3
SpindlePole -4.1 -4.3 -5.8 0 -3.3 – 0 0 0 0 0 -2.8 0.8 -1.2 -0.1
Vacuole 2.3 2.3 0.7 0.6 -0.5 – -0.3 1.1 1.1 0 0 0 4.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3645 3.6255 3.5357 3.329 3.4021 3.6158 5.5265 6.7186 5.9873 5.9109 5.1468 5.8066 5.7698 7.0478 6.3973 7.0864 6.8103 6.643
Actin 0.0031 0.011 0.0114 0.0017 0.0065 0.0179 0.0283 0.0175 0.0134 0.001 0.031 0.0262 0.0054 0.0116 0.0099 0.0015 0.0022 0.0073
Bud 0.0003 0.0013 0.0002 0.0003 0.0001 0.0003 0.0002 0.0003 0.0004 0 0.0011 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0003 0.0007 0.0064 0.0003 0.0004 0.0012 0.0004 0.0003 0.0008 0.0002 0.0003 0.0008 0.0002 0.0003 0.0005 0.0037 0.0002 0.0003
Bud Periphery 0.0002 0.0009 0.0002 0.0003 0.0001 0.0003 0.0002 0.0002 0.0004 0 0.0011 0.0003 0.0001 0.0006 0.0003 0.0002 0.0001 0.0002
Bud Site 0.0003 0.0052 0.0015 0.0007 0.0004 0.0007 0.0007 0.001 0.0025 0.0001 0.0027 0.0004 0.0002 0.0012 0.0013 0.0003 0.0001 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasm 0.0083 0.0139 0.0025 0.0018 0.0019 0.0034 0.0092 0.0024 0.0063 0.0005 0.0056 0.0025 0.0029 0.0025 0.0022 0.001 0.0042 0.0018
Cytoplasmic Foci 0.1684 0.2193 0.1863 0.162 0.1546 0.1379 0.1427 0.1908 0.1848 0.1022 0.1334 0.1404 0.1823 0.2052 0.1775 0.2166 0.2055 0.1633
Eisosomes 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0014 0.0002 0.0003 0.0006 0.0008 0.0012 0.0008 0.0019 0.0001 0.0005 0.0006 0.0004 0.0007 0.0003 0.0001 0.0005 0.0002
Endosome 0.2376 0.2349 0.2303 0.2569 0.2908 0.1971 0.2562 0.2082 0.2718 0.2443 0.1575 0.1898 0.2469 0.236 0.2169 0.2247 0.2759 0.168
Golgi 0.2909 0.3349 0.3775 0.4457 0.3894 0.4097 0.2754 0.3919 0.282 0.305 0.2137 0.3759 0.3512 0.3877 0.383 0.4335 0.3628 0.4232
Lipid Particles 0.1064 0.0429 0.0401 0.0369 0.0253 0.0541 0.109 0.0451 0.0572 0.1532 0.1501 0.0986 0.0682 0.0274 0.0444 0.0121 0.0441 0.0553
Mitochondria 0.0175 0.0275 0.0158 0.0355 0.0153 0.0362 0.0161 0.0214 0.0304 0.0072 0.0147 0.0396 0.0174 0.007 0.0196 0.0071 0.0064 0.0187
None 0.0077 0.0026 0.0012 0.0001 0.0001 0.0027 0.0027 0.0004 0.0074 0 0.0866 0.007 0.0019 0.0038 0.0011 0.0001 0.0058 0.0002
Nuclear Periphery 0.0038 0.0006 0.0001 0.0001 0.0001 0.0009 0.0046 0.0008 0.0008 0.0001 0.0006 0.0003 0.0001 0.0003 0.0002 0 0.0002 0.0001
Nucleolus 0.0054 0.0015 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0
Nucleus 0.0039 0.0005 0.0001 0.0001 0 0.0003 0.0018 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0.0001 0 0 0
Peroxisomes 0.139 0.093 0.1237 0.0527 0.1111 0.1327 0.1365 0.1148 0.1346 0.1831 0.1969 0.1125 0.1199 0.1118 0.14 0.097 0.0899 0.1593
Punctate Nuclear 0.0017 0.0025 0.0006 0.0017 0.0002 0.0007 0.0108 0.0016 0.0008 0.0002 0.0015 0.0012 0.0004 0.0006 0.0005 0.0003 0.0003 0.0002
Vacuole 0.0018 0.0034 0.001 0.0014 0.0014 0.0016 0.002 0.0011 0.0021 0.0014 0.0012 0.0014 0.0012 0.0017 0.001 0.0009 0.0009 0.0006
Vacuole Periphery 0.0015 0.0017 0.0009 0.0015 0.0016 0.0014 0.0017 0.0011 0.002 0.0013 0.0011 0.0019 0.001 0.001 0.0009 0.0006 0.0008 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.7038 35.0564 28.4778 37.5682 23.014 24.3415 51.7691 53.2608 48.2422 46.3067
Translational Efficiency 1.0222 1.0496 0.953 0.7417 0.8647 1.1418 0.7721 0.5826 0.601 0.5678

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
Localization
Cell Percentages Golgi (26%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Stv1

Stv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available