Standard name
Human Ortholog
Description Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.05 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.28 0.18 0.19 0.11 0.07 0.06 0 0.05 0.05 0 0 0.61 0.64 0.78 0.74 0.84 0.84 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.06 0.09 0.05 0 0 0 0 0 0
Endosome 0 0.08 0.11 0.09 0.06 0 0 0 0 0 0 0 0 0.1 0.05 0.05 0.05 0 0.05 0.09 0.09 0.11 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.84 0.57 0.7 0.77 0.82 0.92 0.93 0.94 0.96 0.94 0.95 0.96 0.1 0.11 0 0.1 0.07 0 0.73 0.66 0.69 0.65 0.68 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0
SpindlePole 0 0.05 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0.05 0 0 0 0 0 0 0 0 0.12 0.09 0 0 0 0 0.07 0 0.06 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 10 1 1 1 2 2 7 9 9 2 10 10
Bud 10 5 11 8 12 26 32 29 30 29 22 25 5 1 2 1 1 1 0 4 1 0 4 4
Bud Neck 1 2 0 0 0 0 2 0 0 2 0 0 4 1 5 1 3 5 2 3 4 3 2 10
Bud Site 9 0 2 0 1 8 10 6 8 15 5 12 0 0 0 0 0 1 – – – – – –
Cell Periphery 6 11 2 5 3 2 1 7 1 5 1 4 7 3 2 5 1 2 0 1 0 0 0 1
Cytoplasm 26 79 55 43 36 35 38 23 29 31 19 23 101 126 202 124 92 98 2 19 4 2 5 12
Endoplasmic Reticulum 1 7 2 0 8 7 6 1 4 0 3 2 14 8 8 10 10 6 1 1 1 1 7 4
Endosome 6 22 35 20 19 3 0 2 1 0 0 1 7 19 12 9 6 2 13 33 31 22 18 15
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 4 0 2 2 0 1 12 6 3 6 5 6
Mitochondria 222 161 218 176 267 449 566 621 552 556 443 581 17 21 5 16 8 5 209 247 236 131 215 220
Nucleus 4 0 0 1 4 14 20 18 17 17 5 9 1 1 2 1 0 1 0 1 0 0 2 1
Nuclear Periphery 4 3 0 0 1 5 9 3 3 8 7 6 0 0 0 0 1 0 2 0 2 3 1 1
Nucleolus 4 0 2 0 1 6 5 10 8 6 6 10 0 0 0 0 0 1 1 1 1 3 0 1
Peroxisomes 4 2 55 4 8 2 3 5 8 7 1 2 0 7 4 2 0 0 13 4 24 5 7 8
SpindlePole 11 13 20 16 15 14 24 28 24 26 17 14 6 7 10 10 0 3 3 6 6 1 4 6
Vac/Vac Membrane 9 30 13 6 17 13 17 20 16 21 14 8 6 24 23 3 1 5 8 26 4 12 29 41
Unique Cell Count 263 282 310 230 326 487 607 660 575 594 468 606 165 198 258 167 110 116 289 376 341 202 319 354
Labelled Cell Count 318 337 415 279 392 584 733 773 701 723 543 697 182 219 278 185 125 133 289 376 341 202 319 354


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.5 3.9 3.8 3.6 3.7 3.1 3.3 3.1 3.2 3.1 3.2 5.3 5.1 5.2 5.4 5.0 5.1 6.0 6.1 6.1
Std Deviation (1e-4) 1.6 0.9 0.9 1.2 0.8 1.0 0.8 0.9 0.7 0.8 0.7 0.7 0.7 1.0 1.0 1.8 1.5 1.6 1.4 1.6 1.6
Intensity Change (Log2) – – – -0.07 -0.13 -0.1 -0.36 -0.27 -0.33 -0.32 -0.33 -0.29 0.43 0.37 0.41 0.44 0.35 0.37 0.61 0.62 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – -1.4 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2888 0.2201 -0.023 -0.1506 -0.495 0.1082 2.1035 2.1877 2.3179 2.2902 1.9272 2.3896 -0.2774 0.1145 -0.2036 0.0947 0.1844 -0.2265
Actin 0.0064 0.0063 0.0033 0.003 0.0015 0.0025 0.0299 0.0376 0.0316 0.0342 0.0285 0.0113 0.0077 0.0061 0.0133 0.0022 0.0313 0.0059
Bud 0.0007 0.004 0.0023 0.0029 0.0011 0.0025 0.0018 0.0079 0.0055 0.0092 0.0041 0.0024 0.0017 0.0035 0.0043 0.0011 0.0038 0.001
Bud Neck 0.002 0.0039 0.0021 0.0145 0.0012 0.0049 0.002 0.0041 0.0141 0.002 0.0017 0.0113 0.006 0.0022 0.0027 0.0013 0.0013 0.0045
Bud Periphery 0.0027 0.01 0.0085 0.0122 0.0035 0.0034 0.006 0.0158 0.0088 0.019 0.0184 0.0103 0.0059 0.0107 0.0146 0.0027 0.0146 0.0028
Bud Site 0.007 0.0433 0.0062 0.0028 0.0016 0.0017 0.0149 0.0435 0.0177 0.0128 0.0073 0.0024 0.007 0.0289 0.0219 0.0016 0.0041 0.0011
Cell Periphery 0.0015 0.0022 0.0012 0.0011 0.0003 0.0006 0.0027 0.0021 0.0016 0.001 0.0059 0.002 0.0014 0.0023 0.0036 0.0006 0.0005 0.0007
Cytoplasm 0.0727 0.0962 0.088 0.0953 0.0623 0.0996 0.0513 0.1125 0.0557 0.0446 0.0375 0.0644 0.0578 0.093 0.0719 0.0346 0.0964 0.0975
Cytoplasmic Foci 0.1149 0.1146 0.1121 0.1084 0.1281 0.1265 0.1277 0.1584 0.1289 0.1033 0.0863 0.1332 0.1446 0.1333 0.1181 0.1355 0.1092 0.1455
Eisosomes 0.0011 0.0015 0.0008 0.0009 0.0003 0.0003 0.002 0.0011 0.0012 0.0005 0.0009 0.0009 0.0014 0.0009 0.0013 0.0002 0.0008 0.0005
Endoplasmic Reticulum 0.0027 0.0057 0.0039 0.003 0.0028 0.004 0.0063 0.0023 0.0026 0.0035 0.0031 0.0018 0.0038 0.0024 0.003 0.002 0.0021 0.0026
Endosome 0.0919 0.1051 0.099 0.1185 0.1713 0.1184 0.1336 0.0969 0.1128 0.1277 0.0655 0.1141 0.1359 0.1127 0.0932 0.1307 0.1172 0.1003
Golgi 0.0194 0.0184 0.0183 0.023 0.0264 0.0213 0.0475 0.057 0.0435 0.0587 0.0396 0.0487 0.0409 0.0307 0.0264 0.0524 0.0336 0.0276
Lipid Particles 0.0317 0.0146 0.0201 0.0269 0.0495 0.0225 0.0311 0.0107 0.018 0.0105 0.0141 0.0148 0.0516 0.0167 0.0236 0.0159 0.0229 0.0263
Mitochondria 0.1327 0.1863 0.1903 0.1368 0.2078 0.1992 0.2806 0.1762 0.2928 0.29 0.468 0.326 0.1916 0.246 0.2515 0.3376 0.2328 0.289
None 0.4475 0.3307 0.3938 0.3541 0.2552 0.3175 0.1907 0.1799 0.1831 0.1452 0.1342 0.1803 0.2482 0.2588 0.2693 0.1893 0.2259 0.2056
Nuclear Periphery 0.0021 0.0036 0.0021 0.0019 0.0033 0.002 0.0025 0.0005 0.0026 0.001 0.0066 0.0008 0.0016 0.0008 0.0012 0.0006 0.0021 0.0016
Nucleolus 0.002 0.0026 0.0018 0.0026 0.0019 0.0022 0.0014 0.0013 0.0014 0.0012 0.0009 0.0007 0.0026 0.002 0.0018 0.0009 0.0024 0.0018
Nucleus 0.0021 0.0041 0.0024 0.0045 0.0045 0.0053 0.0013 0.0017 0.0018 0.0022 0.0016 0.001 0.0017 0.0024 0.0027 0.0011 0.0029 0.0025
Peroxisomes 0.0389 0.0208 0.0264 0.0581 0.0466 0.0414 0.0486 0.0789 0.0601 0.1215 0.0613 0.0625 0.0624 0.0297 0.0558 0.0719 0.0452 0.0687
Punctate Nuclear 0.0029 0.0049 0.0039 0.0048 0.0124 0.0056 0.002 0.0033 0.0014 0.0008 0.0022 0.0014 0.0056 0.0009 0.0023 0.0008 0.033 0.0037
Vacuole 0.0139 0.0161 0.01 0.0182 0.0126 0.013 0.0124 0.0064 0.0109 0.0083 0.0077 0.0063 0.0162 0.0121 0.0133 0.01 0.012 0.0073
Vacuole Periphery 0.0031 0.0049 0.0037 0.0064 0.0058 0.0057 0.0039 0.0018 0.0038 0.0027 0.0046 0.0034 0.0045 0.004 0.0041 0.007 0.0059 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1187 4.2425 7.8703 4.85 8.4606 10.6722 7.5067 8.3236 9.2564 7.0017
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
Localization
Cell Percentages mitochondrion (55%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Aac1

Aac1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available