Standard name | |
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Human Ortholog | |
Description | Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 1 | 6 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2 | 0 | 1 | 2 | – | 4 | 4 | 10 | 7 | 0 | 0 | 0 | 1 | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 7 | 5 | 5 | 8 | 15 | 17 | – | 8 | 9 | 5 | 10 | 16 | 34 | 52 | 6 | – | – | 0 | 0 | 1 | 2 | 6 | 3 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 3 | 1 | 2 | 4 | 4 | – | 0 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | – | – | 1 | 2 | 2 | 1 | 3 | 7 |
Golgi | 0 | 0 | 3 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | – | 1 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 18 | 7 | 0 | 1 | 3 | 10 | 21 | – | 53 | 39 | 128 | 48 | 0 | 2 | 0 | 2 | – | – | 0 | 2 | 1 | 1 | 2 | 1 |
Nucleus | 100 | 334 | 148 | 134 | 208 | 228 | 301 | – | 182 | 300 | 249 | 229 | 159 | 198 | 229 | 26 | – | – | 87 | 346 | 133 | 139 | 196 | 212 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 2 | 3 | 1 | – | 1 | 5 | 2 | 3 | 0 | 1 | 1 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 31 | 51 | 8 | 6 | 9 | 36 | 36 | – | 36 | 38 | 72 | 63 | 5 | 21 | 21 | 7 | – | – | 15 | 45 | 18 | 11 | 23 | 21 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 7 | 1 | 4 | 3 | 6 | – | 4 | 10 | 3 | 9 | 1 | 2 | 5 | 1 | – | – | 0 | 3 | 2 | 0 | 5 | 7 |
Vac/Vac Membrane | 1 | 10 | 4 | 1 | 3 | 1 | 7 | – | 2 | 1 | 0 | 4 | 2 | 1 | 1 | 2 | – | – | 1 | 7 | 8 | 3 | 10 | 11 |
Unique Cell Count | 106 | 374 | 161 | 145 | 229 | 273 | 358 | 233 | 352 | 330 | 296 | 178 | 244 | 297 | 40 | 110 | 418 | 175 | 164 | 257 | 277 | |||
Labelled Cell Count | 150 | 412 | 179 | 152 | 241 | 301 | 398 | 293 | 408 | 476 | 376 | 185 | 261 | 317 | 47 | 110 | 418 | 175 | 164 | 257 | 277 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 7.9 | 7.7 | 7.6 | 7.6 | 6.3 | 6.7 | – | 5.7 | 5.6 | 4.7 | 5.4 | 8.1 | 8.2 | 7.9 | 9.2 | – | – | 7.1 | 7.7 | 7.9 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.6 | 2.2 | 1.9 | 1.7 | 2.7 | – | 2.1 | 1.1 | 0.8 | 1.4 | 1.7 | 1.9 | 1.9 | 2.9 | – | – | 1.0 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.02 | -0.28 | -0.2 | – | -0.43 | -0.45 | -0.7 | -0.52 | 0.08 | 0.09 | 0.04 | 0.26 | – | – | -0.11 | 0.0 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | 0 | 0.2 | 1.1 | 0.9 | – | 0.2 | 0 | 0 | 0.2 | 2.2 | 3.6 | 4.5 | 0 | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 0 | 0 | 0 | 3.1 | – | 6.5 | 4.4 | 0 | 5.4 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0.2 | -0.4 | -2.5 | -2.4 | – | -3.7 | -2.1 | -4.4 | -3.9 | -0.8 | -3.0 | -4.0 | -4.5 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | -0.3 | -0.5 | 2.7 | 1.9 | – | 3.2 | 2.1 | 0 | 4.6 | -1.0 | 1.4 | 0.9 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | -0.9 | 0 | -0.7 | 0 | 0 | 0 | 0 | – | – |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5593 | 2.439 | 2.3468 | 1.6755 | 1.2427 | 2.2926 | 2.0557 | 3.0875 | 2.2522 | 1.9953 | 1.7301 | 2.1161 | 3.3713 | 3.5975 | 3.135 | 3.3956 | 3.2437 | 3.1672 |
Actin | 0.0159 | 0.0001 | 0.0055 | 0.0167 | 0 | 0.0005 | 0.0142 | 0 | 0.0003 | 0.0629 | 0 | 0 | 0.0321 | 0.0001 | 0.005 | 0.0535 | 0.0005 | 0 |
Bud | 0.0004 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0.0005 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 |
Bud Neck | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0008 | 0.0008 | 0.0001 | 0.0005 | 0.0049 | 0.0002 | 0.0006 | 0.0015 | 0 | 0.0006 | 0.0029 | 0.0006 | 0.0005 |
Bud Periphery | 0.0004 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0013 | 0.0001 | 0 | 0.0011 | 0 | 0.0005 | 0.0005 | 0.0003 | 0 |
Bud Site | 0.0015 | 0 | 0.001 | 0.001 | 0.0003 | 0.0001 | 0.0022 | 0 | 0.0003 | 0.0033 | 0.0001 | 0 | 0.0017 | 0 | 0.0028 | 0.0007 | 0.0004 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0216 | 0.0002 | 0.0035 | 0.0005 | 0 | 0.0126 | 0.0012 | 0 | 0.0005 | 0.0019 | 0 | 0 | 0.0054 | 0.0014 | 0.0015 | 0.0031 | 0.0003 | 0.0002 |
Cytoplasmic Foci | 0.0302 | 0 | 0.0162 | 0.009 | 0 | 0.0123 | 0.0052 | 0 | 0.0003 | 0.0603 | 0 | 0 | 0.0151 | 0.0007 | 0.0046 | 0.0025 | 0.0107 | 0.0002 |
Eisosomes | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0.0003 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0031 | 0 | 0.0013 | 0.0021 | 0 | 0.0012 | 0.0014 | 0 | 0.0002 | 0.006 | 0 | 0 | 0.0053 | 0.0003 | 0.0003 | 0.0021 | 0.0001 | 0.0001 |
Endosome | 0.0227 | 0.0001 | 0.0201 | 0.0224 | 0.0001 | 0.002 | 0.0155 | 0 | 0.0004 | 0.0425 | 0 | 0 | 0.0198 | 0.0009 | 0.0023 | 0.0145 | 0.0094 | 0.0004 |
Golgi | 0.0082 | 0 | 0.0082 | 0.0237 | 0 | 0.0009 | 0.0079 | 0 | 0.0001 | 0.0318 | 0 | 0 | 0.0062 | 0 | 0.0014 | 0.0051 | 0.0026 | 0 |
Lipid Particles | 0.0131 | 0 | 0.0155 | 0.0249 | 0.0001 | 0.0044 | 0.0094 | 0 | 0.0001 | 0.0439 | 0 | 0 | 0.0184 | 0.0002 | 0.0079 | 0.0026 | 0.0111 | 0.0001 |
Mitochondria | 0.005 | 0.0113 | 0.0354 | 0.0807 | 0.0008 | 0.0002 | 0.0033 | 0.0001 | 0.0003 | 0.0127 | 0.0004 | 0.0001 | 0.0056 | 0.0001 | 0.004 | 0.0029 | 0.0021 | 0.0001 |
None | 0.0014 | 0.0001 | 0.0032 | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.001 | 0 | 0 | 0.0071 | 0.0037 | 0.0005 | 0.002 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.0141 | 0.0012 | 0.0042 | 0.0148 | 0.0005 | 0.0048 | 0.0081 | 0.0007 | 0.0033 | 0.0173 | 0.0006 | 0.0001 | 0.0249 | 0.0031 | 0.0017 | 0.0092 | 0.0032 | 0.0017 |
Nucleolus | 0.0513 | 0.0188 | 0.0337 | 0.0084 | 0.3841 | 0.0982 | 0.0274 | 0.0297 | 0.0191 | 0.0434 | 0.1622 | 0.0776 | 0.0323 | 0.0248 | 0.0299 | 0.0085 | 0.1893 | 0.086 |
Nucleus | 0.7387 | 0.9619 | 0.8313 | 0.7814 | 0.6053 | 0.834 | 0.8903 | 0.9689 | 0.9687 | 0.5911 | 0.8341 | 0.9206 | 0.7786 | 0.9594 | 0.916 | 0.8825 | 0.7497 | 0.9083 |
Peroxisomes | 0.0148 | 0 | 0.0128 | 0.0085 | 0 | 0.0037 | 0.0024 | 0 | 0.0001 | 0.0624 | 0 | 0 | 0.0074 | 0 | 0.0038 | 0.001 | 0.0095 | 0 |
Punctate Nuclear | 0.0511 | 0.005 | 0.003 | 0.0019 | 0.007 | 0.0234 | 0.0028 | 0.0004 | 0.0052 | 0.0078 | 0.0022 | 0.0009 | 0.0293 | 0.0048 | 0.0137 | 0.0047 | 0.0087 | 0.0022 |
Vacuole | 0.0037 | 0.0003 | 0.0019 | 0.0006 | 0.0003 | 0.0003 | 0.0039 | 0 | 0.0001 | 0.0021 | 0.0001 | 0 | 0.0044 | 0.0002 | 0.0023 | 0.0005 | 0.0004 | 0.0001 |
Vacuole Periphery | 0.0022 | 0.0006 | 0.0017 | 0.0014 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0024 | 0 | 0 | 0.0025 | 0.0001 | 0.0005 | 0.0006 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.3266 | 28.1268 | 11.875 | 27.1953 | 23.2105 | 21.145 | 20.7163 | 17.8706 | 19.3476 | 29.014 |
Translational Efficiency | 1.0598 | 0.7824 | 1.721 | 0.7221 | 0.9492 | 1.0787 | 0.8434 | 0.9984 | 1.0655 | 0.8905 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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