Standard name | |
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Human Ortholog | |
Description | N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 2 |
Bud | 0 | 1 | 0 | 1 | 5 | 6 | 9 | 2 | 6 | 5 | 14 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 3 | 11 | 9 | 14 | 18 |
Bud Neck | 0 | 0 | 3 | 0 | 9 | 1 | 11 | 3 | 2 | 5 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 2 | 4 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 5 | 4 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Cytoplasm | 132 | 136 | 270 | 122 | 151 | 235 | 210 | 273 | 90 | 85 | 92 | 90 | 150 | 226 | 268 | 83 | 54 | 43 | 110 | 135 | 249 | 97 | 245 | 221 |
Endoplasmic Reticulum | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 14 | 10 | 3 | 0 | 0 | 1 | 4 | 1 | 2 |
Endosome | 0 | 5 | 14 | 7 | 3 | 11 | 0 | 11 | 1 | 2 | 3 | 2 | 8 | 3 | 9 | 5 | 0 | 2 | 5 | 3 | 4 | 1 | 1 | 4 |
Golgi | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 4 | 2 | 0 | 0 | 0 |
Mitochondria | 12 | 44 | 9 | 8 | 57 | 105 | 188 | 89 | 244 | 178 | 260 | 220 | 0 | 0 | 1 | 12 | 3 | 5 | 1 | 9 | 12 | 2 | 2 | 1 |
Nucleus | 39 | 42 | 53 | 45 | 25 | 103 | 68 | 162 | 113 | 120 | 157 | 191 | 5 | 2 | 2 | 66 | 64 | 41 | 88 | 122 | 139 | 29 | 27 | 8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 1 | 0 | 2 | 6 | 13 | 0 | 6 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 9 | 27 | 6 | 11 | 11 |
Vac/Vac Membrane | 89 | 100 | 155 | 31 | 29 | 69 | 4 | 100 | 20 | 19 | 32 | 39 | 35 | 63 | 63 | 84 | 73 | 64 | 33 | 53 | 67 | 19 | 28 | 14 |
Unique Cell Count | 226 | 278 | 439 | 205 | 244 | 465 | 400 | 568 | 370 | 347 | 434 | 433 | 185 | 271 | 306 | 210 | 172 | 129 | 263 | 353 | 541 | 182 | 349 | 302 |
Labelled Cell Count | 276 | 330 | 508 | 219 | 279 | 532 | 499 | 657 | 484 | 425 | 565 | 567 | 199 | 295 | 346 | 270 | 204 | 159 | 263 | 353 | 541 | 182 | 349 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.2 | 6.0 | 5.5 | 4.5 | 4.6 | 3.4 | 5.6 | 3.8 | 4.2 | 4.1 | 4.2 | 5.6 | 5.4 | 6.1 | 7.0 | 8.1 | 9.1 | 13.9 | 11.4 | 10.5 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.4 | 2.0 | 1.7 | 1.3 | 1.1 | 2.7 | 1.0 | 0.9 | 0.9 | 1.0 | 1.2 | 1.1 | 6.4 | 1.7 | 1.5 | 1.7 | 21.4 | 19.7 | 16.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.43 | -0.39 | -0.81 | -0.1 | -0.68 | -0.52 | -0.55 | -0.5 | -0.11 | -0.14 | 0.01 | 0.22 | 0.44 | 0.61 | 1.21 | 0.93 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.2 | 1.6 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6243 | 2.9829 | 2.7664 | 2.3829 | 2.9499 | 2.8165 | 3.979 | 4.328 | 4.1491 | 4.3194 | 4.0566 | 4.1048 | 2.481 | 2.7408 | 2.3107 | 2.1967 | 2.3185 | 2.2108 |
Actin | 0.0315 | 0.0004 | 0.0161 | 0.0091 | 0.0022 | 0.0004 | 0.013 | 0.0001 | 0.004 | 0.0002 | 0.0003 | 0.0007 | 0.0287 | 0.0002 | 0.0029 | 0.0045 | 0.0028 | 0.0003 |
Bud | 0.0007 | 0.0028 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0067 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0066 | 0.0008 | 0.0006 | 0.0175 | 0.0019 | 0.0041 | 0.0008 | 0.0003 | 0.0012 | 0.0002 | 0.0013 | 0.0019 | 0.0024 | 0.0004 | 0.0005 | 0.0007 | 0.0006 | 0.0028 |
Bud Periphery | 0.0007 | 0.0006 | 0.001 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0018 | 0 | 0 | 0 | 0.0012 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0109 | 0.0095 | 0.0015 | 0.0137 | 0.0006 | 0.0003 | 0.0057 | 0.0006 | 0.0012 | 0.0001 | 0.0002 | 0.0002 | 0.0041 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0002 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0011 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2547 | 0.3212 | 0.1926 | 0.1465 | 0.2133 | 0.2443 | 0.1796 | 0.2629 | 0.1537 | 0.0669 | 0.2399 | 0.2212 | 0.1923 | 0.2311 | 0.1607 | 0.2037 | 0.2001 | 0.2535 |
Cytoplasmic Foci | 0.0299 | 0.0049 | 0.0054 | 0.01 | 0.0185 | 0.0021 | 0.0482 | 0.0011 | 0.0088 | 0.0035 | 0.003 | 0.0098 | 0.0159 | 0.0018 | 0.0056 | 0.0142 | 0.0139 | 0.0056 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0016 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0052 | 0.0046 | 0.0031 | 0.0022 | 0.005 | 0.0031 | 0.0072 | 0.0027 | 0.0047 | 0.001 | 0.0042 | 0.0022 | 0.0111 | 0.0022 | 0.0021 | 0.006 | 0.0027 | 0.0023 |
Endosome | 0.0194 | 0.0024 | 0.0085 | 0.0124 | 0.0229 | 0.002 | 0.0329 | 0.0013 | 0.0048 | 0.001 | 0.0048 | 0.0046 | 0.0169 | 0.0014 | 0.0037 | 0.003 | 0.0086 | 0.0036 |
Golgi | 0.0089 | 0.0002 | 0.0072 | 0.0072 | 0.009 | 0 | 0.0089 | 0 | 0.0028 | 0.0001 | 0.0001 | 0.0003 | 0.006 | 0 | 0.0005 | 0.0005 | 0.0068 | 0.0001 |
Lipid Particles | 0.0215 | 0.0004 | 0.0065 | 0.0079 | 0.0333 | 0.0001 | 0.0135 | 0.0001 | 0.0072 | 0.0006 | 0.0002 | 0.0009 | 0.0148 | 0.0001 | 0.0004 | 0.0022 | 0.0076 | 0.0002 |
Mitochondria | 0.0067 | 0.0004 | 0.0051 | 0.0165 | 0.0456 | 0.0005 | 0.0059 | 0.0003 | 0.0149 | 0.0002 | 0.0006 | 0.0004 | 0.0056 | 0.0005 | 0.003 | 0.0007 | 0.0036 | 0.0006 |
None | 0.156 | 0.1926 | 0.1621 | 0.1203 | 0.1779 | 0.1391 | 0.1384 | 0.2546 | 0.1675 | 0.2339 | 0.2213 | 0.2024 | 0.1936 | 0.2299 | 0.2077 | 0.1669 | 0.2201 | 0.1521 |
Nuclear Periphery | 0.0104 | 0.0084 | 0.0138 | 0.0088 | 0.0131 | 0.0068 | 0.018 | 0.0091 | 0.0131 | 0.0081 | 0.0108 | 0.0076 | 0.016 | 0.0079 | 0.0104 | 0.0119 | 0.0095 | 0.0083 |
Nucleolus | 0.004 | 0.0023 | 0.0028 | 0.0025 | 0.0012 | 0.003 | 0.0032 | 0.0009 | 0.0017 | 0.0019 | 0.0026 | 0.0017 | 0.0028 | 0.0015 | 0.0019 | 0.0015 | 0.0023 | 0.0027 |
Nucleus | 0.3735 | 0.412 | 0.5568 | 0.5949 | 0.4368 | 0.5705 | 0.4516 | 0.458 | 0.5823 | 0.6605 | 0.4944 | 0.5058 | 0.4313 | 0.5063 | 0.5663 | 0.548 | 0.4746 | 0.5371 |
Peroxisomes | 0.015 | 0.0003 | 0.0056 | 0.0051 | 0.0101 | 0.0001 | 0.0395 | 0 | 0.0082 | 0.0001 | 0.0001 | 0.0082 | 0.0151 | 0.0001 | 0.0019 | 0.0106 | 0.0258 | 0.0002 |
Punctate Nuclear | 0.0404 | 0.0334 | 0.0081 | 0.0223 | 0.0042 | 0.0213 | 0.0263 | 0.0055 | 0.0135 | 0.0212 | 0.014 | 0.0309 | 0.0346 | 0.014 | 0.0298 | 0.0237 | 0.0189 | 0.0285 |
Vacuole | 0.0029 | 0.0024 | 0.0017 | 0.0014 | 0.0024 | 0.0016 | 0.0046 | 0.0021 | 0.0013 | 0.0003 | 0.0017 | 0.0009 | 0.0034 | 0.0015 | 0.001 | 0.0009 | 0.001 | 0.0015 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0007 | 0.0006 | 0.0011 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2882 | 10.3466 | 5.9715 | 4.6793 | 5.0021 | 3.8559 | 4.6495 | 6.6685 | 4.2999 | 9.572 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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