| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 2 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | – | – | – | – | 0 | 1 | 0 | 3 | 0 | 2 |
| Bud | – | 4 | – | – | – | – | – | – | – | – | – | – | 1 | 1 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
| Bud Neck | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 1 | 0 | 1 | 6 | 2 |
| Bud Site | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 41 | – | – | – | – | – | – | – | – | – | – | 14 | 8 | – | – | – | – | 0 | 22 | 4 | 9 | 20 | 23 |
| Endoplasmic Reticulum | – | 4 | – | – | – | – | – | – | – | – | – | – | 1 | 0 | – | – | – | – | 0 | 1 | 1 | 5 | 4 | 3 |
| Endosome | – | 6 | – | – | – | – | – | – | – | – | – | – | 6 | 4 | – | – | – | – | 0 | 30 | 6 | 22 | 19 | 13 |
| Golgi | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 2 | 0 | 1 | 0 | 2 |
| Mitochondria | – | 16 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 12 | 2 | 7 | 6 | 5 |
| Nucleus | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
| Nuclear Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 1 | 0 | 0 | 1 | 1 |
| Nucleolus | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 3 | – | – | – | – | – | – | – | – | – | – | 0 | 4 | – | – | – | – | 0 | 6 | 1 | 5 | 3 | 1 |
| Vac/Vac Membrane | – | 2 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – | 0 | 16 | 0 | 6 | 9 | 9 |
| Unique Cell Count | 70 | 20 | 16 | 2 | 107 | 23 | 65 | 77 | 80 | |||||||||||||||
| Labelled Cell Count | 78 | 22 | 19 | 2 | 107 | 23 | 65 | 77 | 80 | |||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 5.0 | – | – | – | – | – | – | – | – | – | – | 5.9 | 8.0 | – | – | – | – | 5.3 | 5.5 | 6.4 |
| Std Deviation (1e-4) | – | 0.9 | – | – | – | – | – | – | – | – | – | – | 1.6 | 2.4 | – | – | – | – | 2.2 | 1.1 | 2.3 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 0.0714 | 0.7684 | 0.5875 | 0.2903 | 0.3201 | 0.7662 | 1.8289 | 2.1709 | 1.8195 | 1.4349 | 1.9202 | 1.5438 | 4.2763 | 5.3564 | 4.3522 | 4.5566 | 3.8087 | 4.6813 |
| Actin | 0.0115 | 0.0019 | 0.0043 | 0.0001 | 0.0085 | 0.0002 | 0.0344 | 0.0003 | 0.0013 | 0.0216 | 0.0055 | 0.0001 | 0.0069 | 0.0003 | 0.0167 | 0.0001 | 0.0018 | 0.0003 |
| Bud | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0 | 0.0008 | 0.0022 | 0.0002 | 0.0002 | 0.0068 | 0 | 0.0002 | 0.0022 | 0.0002 | 0 | 0.0001 | 0.0001 |
| Bud Neck | 0.0005 | 0.0009 | 0.0004 | 0.0003 | 0.0017 | 0.0005 | 0.003 | 0.0018 | 0.0011 | 0.0003 | 0.0006 | 0.0013 | 0.001 | 0.0003 | 0.002 | 0.0001 | 0.0007 | 0.0004 |
| Bud Periphery | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0013 | 0.0017 | 0.0003 | 0.0002 | 0.0193 | 0 | 0.0004 | 0.0012 | 0.0005 | 0 | 0.0003 | 0.0001 |
| Bud Site | 0.0019 | 0.0018 | 0.0025 | 0.0001 | 0.0008 | 0.0002 | 0.0084 | 0.0121 | 0.0013 | 0.0005 | 0.0069 | 0.0002 | 0.001 | 0.0028 | 0.0009 | 0.0001 | 0.0016 | 0.0002 |
| Cell Periphery | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 |
| Cytoplasm | 0.0344 | 0.0463 | 0.0364 | 0.0302 | 0.0011 | 0.0422 | 0.0429 | 0.0055 | 0.0018 | 0.0008 | 0.0012 | 0.0351 | 0.0068 | 0.0287 | 0.0039 | 0.0004 | 0.0006 | 0.0003 |
| Cytoplasmic Foci | 0.2742 | 0.2151 | 0.1756 | 0.1698 | 0.1475 | 0.1943 | 0.174 | 0.204 | 0.2064 | 0.2172 | 0.1539 | 0.2277 | 0.1331 | 0.2569 | 0.1867 | 0.1598 | 0.1157 | 0.1612 |
| Eisosomes | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 |
| Endoplasmic Reticulum | 0.005 | 0.0016 | 0.0017 | 0.0003 | 0.0037 | 0.0008 | 0.0062 | 0.0003 | 0.0008 | 0.0008 | 0.0012 | 0.0016 | 0.006 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0001 |
| Endosome | 0.2028 | 0.2513 | 0.2734 | 0.4242 | 0.1906 | 0.2378 | 0.2356 | 0.2407 | 0.1871 | 0.2816 | 0.285 | 0.2185 | 0.144 | 0.1607 | 0.1234 | 0.2524 | 0.2301 | 0.1324 |
| Golgi | 0.028 | 0.0453 | 0.0521 | 0.0632 | 0.0949 | 0.0507 | 0.027 | 0.0378 | 0.0388 | 0.082 | 0.0523 | 0.0464 | 0.0333 | 0.0321 | 0.0344 | 0.0916 | 0.0374 | 0.0445 |
| Lipid Particles | 0.3631 | 0.2528 | 0.3453 | 0.193 | 0.3426 | 0.3387 | 0.2519 | 0.2832 | 0.4089 | 0.2363 | 0.307 | 0.3331 | 0.3577 | 0.3666 | 0.4349 | 0.2781 | 0.4023 | 0.5221 |
| Mitochondria | 0.0036 | 0.0167 | 0.0062 | 0.0019 | 0.0346 | 0.0118 | 0.0257 | 0.011 | 0.0031 | 0.0028 | 0.0526 | 0.0057 | 0.0074 | 0.0013 | 0.0074 | 0.0013 | 0.0047 | 0.0045 |
| None | 0.0071 | 0.0098 | 0.0039 | 0.0002 | 0.0008 | 0.0036 | 0.0061 | 0.0045 | 0.014 | 0.0002 | 0.0004 | 0.002 | 0.0423 | 0.0268 | 0.0141 | 0.0904 | 0.0005 | 0.0001 |
| Nuclear Periphery | 0.003 | 0.0006 | 0.0005 | 0.0002 | 0.0156 | 0.0003 | 0.014 | 0.0006 | 0.0014 | 0.0002 | 0.0009 | 0.0002 | 0.0076 | 0.0003 | 0.0017 | 0.0001 | 0.0019 | 0.0002 |
| Nucleolus | 0.0005 | 0.0082 | 0.0004 | 0.0002 | 0.011 | 0.0005 | 0.0023 | 0.0235 | 0.0037 | 0.0002 | 0.0007 | 0.0028 | 0.0221 | 0.0012 | 0.0051 | 0.0002 | 0.0032 | 0.0013 |
| Nucleus | 0.0029 | 0.0009 | 0.0003 | 0.0001 | 0.0022 | 0.0002 | 0.0209 | 0.004 | 0.0009 | 0.0001 | 0.001 | 0.0007 | 0.0025 | 0.0005 | 0.002 | 0 | 0.0009 | 0.0001 |
| Peroxisomes | 0.0508 | 0.0939 | 0.0741 | 0.0997 | 0.1163 | 0.1068 | 0.1014 | 0.1071 | 0.1071 | 0.151 | 0.0823 | 0.1129 | 0.1912 | 0.1027 | 0.1236 | 0.1179 | 0.149 | 0.1211 |
| Punctate Nuclear | 0.0072 | 0.0018 | 0.0009 | 0.0008 | 0.0021 | 0.0021 | 0.0205 | 0.0114 | 0.0038 | 0.0012 | 0.0028 | 0.0054 | 0.009 | 0.0034 | 0.0306 | 0.0008 | 0.0176 | 0.0049 |
| Vacuole | 0.0023 | 0.04 | 0.0167 | 0.0133 | 0.0195 | 0.0068 | 0.0177 | 0.0452 | 0.0158 | 0.0019 | 0.0137 | 0.005 | 0.0224 | 0.0101 | 0.0089 | 0.0049 | 0.023 | 0.0049 |
| Vacuole Periphery | 0.0008 | 0.01 | 0.0048 | 0.0023 | 0.005 | 0.0022 | 0.0051 | 0.0027 | 0.0018 | 0.0007 | 0.0045 | 0.001 | 0.0041 | 0.0012 | 0.0015 | 0.0015 | 0.008 | 0.0011 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 10.769 | 5.9242 | 10.99 | 11.0146 | 9.6663 | 4.9675 | 9.7336 | 14.1498 | 10.2878 | 11.6268 |
| Translational Efficiency | 0.9677 | 1.646 | 0.8578 | 1.001 | 0.8865 | 2.7389 | 1.2623 | 0.8271 | 1.3563 | 1.1203 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✘ |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes |
| Localization | |
| Cell Percentages | endosome (13%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Vps20
Vps20
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Vps20-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
