Standard name
Human Ortholog
Description ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0.1 0.14 0.15 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0.13 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.95 0.95 0.9 0.9 0.83 0.8 0.64 0.61 – 0.52 0.5 0.71 0.63 0.54 0.94 0.91 0.87 0.93 0.92 0.9 0.83 0.78 0.71
Endoplasmic Reticulum 0 0.06 0 0 0.05 0 0.09 0.05 0 – 0 0.05 0.1 0 0 0 0 0.05 0 0 0.05 0.1 0.11 0.22
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.15 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0.08 0.16 0.1 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.14 0 0.22 0.2 0.4 0.53 – 0.58 0.6 0 0 0.1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 2 4 4 6 1 – 4 3 15 37 38 0 1 1 0 0 0 0 0 1
Bud 0 0 2 1 1 1 1 3 9 – 7 5 7 14 34 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 5 5 3 6 12 11 11 – 15 15 1 2 3 23 7 8 0 0 0 0 0 0
Cytoplasm 87 148 167 172 178 185 281 259 167 – 126 116 109 166 139 336 155 187 99 146 172 107 133 165
Endoplasmic Reticulum 4 9 5 8 9 6 33 22 6 – 10 12 15 11 4 14 5 11 3 4 10 13 18 51
Endosome 0 0 0 0 1 1 0 1 1 – 0 0 7 17 38 0 0 1 0 0 0 0 2 1
Golgi 0 0 0 0 1 0 0 0 0 – 0 0 13 42 25 4 2 3 0 1 0 0 1 2
Mitochondria 0 1 0 26 8 50 69 161 145 – 142 137 2 5 25 6 0 3 0 0 1 1 3 1
Nucleus 0 0 1 0 0 0 0 3 0 – 0 1 1 1 1 4 3 5 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 – 3 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 0 0 5 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 2 4 5 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 2 1 5 3 4 11 5 – 5 3 2 4 5 8 10 15 0 3 5 2 5 2
Unique Cell Count 90 156 176 191 198 224 353 404 274 244 230 153 265 257 359 171 216 107 160 192 130 171 233
Labelled Cell Count 92 161 182 215 208 256 404 477 346 313 292 174 308 321 395 183 234 107 160 192 130 171 233


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.2 12.7 10.4 9.7 10.2 8.8 8.0 7.3 6.9 – 6.6 6.4 12.8 12.5 10.6 14.8 15.5 16.1 13.5 13.0 14.4
Std Deviation (1e-4) 2.3 2.1 1.3 1.3 1.3 1.3 1.2 1.1 1.2 – 1.3 1.1 1.9 1.8 1.6 2.9 3.2 3.4 2.5 2.9 2.7
Intensity Change (Log2) – – – -0.09 -0.04 -0.24 -0.37 -0.51 -0.58 – -0.65 -0.7 0.3 0.27 0.02 0.51 0.58 0.63 0.38 0.32 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 4.3 5.2 5.3 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 2.3 4.5 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.3 0 0.7 – 1.6 1.7 0 0 0 1.7 0.6 0.5
Cytoplasm -1.7 -1.8 -3.8 -4.6 -7.7 -8.0 – -9.5 -9.7 -5.8 -7.7 -9.2 -0.6 -1.5 -2.8
Endoplasmic Reticulum 0.7 0.9 0 2.7 1.4 0 – 0.7 1.2 2.6 0.7 0 0.6 0 1.1
Endosome 0 0 0 0 0 0 – 0 0 0 3.4 5.3 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 3.9 5.6 4.3 0 0 0
Mitochondria 5.1 0 6.7 6.3 9.9 11.7 – 12.4 12.6 0 0 4.3 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 2.4 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.2963 12.8828 12.4265 11.188 11.8564 12.8528 9.7926 14.6495 13.311 12.5983 11.8316 12.8864 10.7791 15.1521 12.9451 13.7431 13.8091 13.8686
Actin 0.0184 0.0011 0.0126 0.0191 0.0051 0.0025 0.0533 0.0007 0.0159 0.0046 0.0061 0.0091 0.0734 0.0017 0.028 0.0022 0.0039 0.0023
Bud 0.0005 0.0004 0.001 0.001 0.004 0.0006 0.0021 0.0002 0.0004 0.001 0.0003 0.0003 0.0014 0.0004 0.0038 0.0011 0.0006 0.0004
Bud Neck 0.0064 0.0005 0.0009 0.0022 0.0015 0.0029 0.002 0.0003 0.0007 0.0009 0.0006 0.0027 0.0099 0.0002 0.0008 0.0007 0.0007 0.0017
Bud Periphery 0.0005 0.0002 0.0007 0.0006 0.0067 0.0009 0.002 0.0002 0.0003 0.0008 0.0002 0.0003 0.0018 0.0001 0.0017 0.0009 0.0001 0.0002
Bud Site 0.0071 0.0025 0.001 0.0011 0.0005 0.0002 0.0051 0.0021 0.0013 0.0019 0.0002 0.0004 0.0081 0.0029 0.0044 0.0003 0.0002 0.0001
Cell Periphery 0.0008 0.0009 0.0008 0.0003 0.0005 0.0007 0.0008 0.0007 0.0005 0.0004 0.0001 0.0003 0.0011 0.0001 0.0006 0.0011 0 0.0002
Cytoplasm 0.5549 0.8949 0.8046 0.7171 0.7464 0.7531 0.5009 0.9329 0.8625 0.7372 0.8335 0.8355 0.5273 0.9554 0.7499 0.7347 0.8119 0.7949
Cytoplasmic Foci 0.0089 0.0066 0.0081 0.0182 0.0126 0.0133 0.0629 0.0053 0.0251 0.0222 0.0213 0.0188 0.0345 0.012 0.0241 0.0238 0.0349 0.0251
Eisosomes 0.0006 0 0 0.0001 0 0 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0006 0 0.0003 0.0005 0 0
Endoplasmic Reticulum 0.2319 0.0526 0.1055 0.0693 0.1079 0.1325 0.071 0.0364 0.0301 0.0349 0.0194 0.0445 0.1309 0.0043 0.0401 0.0362 0.0162 0.0853
Endosome 0.0453 0.0183 0.0256 0.0761 0.0589 0.051 0.1226 0.0093 0.0296 0.0979 0.0377 0.0547 0.0824 0.0127 0.0667 0.1326 0.0939 0.0575
Golgi 0.0044 0.0024 0.0032 0.0105 0.005 0.0059 0.0303 0.0015 0.0059 0.0063 0.0099 0.0091 0.0255 0.0046 0.0161 0.0123 0.0223 0.0121
Lipid Particles 0.0045 0.0004 0.0006 0.0053 0.0011 0.001 0.0211 0.0004 0.0039 0.0032 0.0021 0.0029 0.0189 0.0001 0.0086 0.0004 0 0.0008
Mitochondria 0.0052 0.0012 0.006 0.0044 0.0048 0.0062 0.0161 0.0004 0.0022 0.0159 0.0484 0.0048 0.0084 0.0004 0.0237 0.0106 0.0051 0.0063
None 0.0118 0.0013 0.0028 0.0028 0.0019 0.0009 0.006 0.0007 0.0009 0.0072 0.0019 0.0008 0.0072 0.0006 0.0048 0.0012 0.0006 0.0008
Nuclear Periphery 0.0321 0.0033 0.0074 0.0156 0.0138 0.0053 0.0207 0.0022 0.0016 0.0038 0.0038 0.0041 0.0154 0.0007 0.0041 0.0044 0.0015 0.0023
Nucleolus 0.0011 0 0.0001 0.0004 0.0001 0.0001 0.0019 0.0001 0.0001 0.0001 0.0001 0.0001 0.0041 0 0.0002 0 0 0
Nucleus 0.0081 0.0017 0.0024 0.0069 0.005 0.0026 0.0156 0.0019 0.0011 0.0024 0.0032 0.002 0.0067 0.0008 0.0018 0.0018 0.0011 0.0012
Peroxisomes 0.0013 0.0001 0.0006 0.0037 0.0004 0.0004 0.0094 0 0.0014 0.0006 0.0024 0.0016 0.013 0.0001 0.0057 0.0002 0.0007 0.0036
Punctate Nuclear 0.0063 0.0001 0.0017 0.0045 0.0032 0.0011 0.0314 0.0001 0.0079 0.0004 0.005 0.0007 0.0171 0.0001 0.001 0.0002 0.0001 0.0008
Vacuole 0.0429 0.0101 0.011 0.0311 0.0155 0.0139 0.0205 0.0041 0.0071 0.0478 0.0022 0.0048 0.0093 0.0022 0.0099 0.0256 0.0033 0.0027
Vacuole Periphery 0.0071 0.0015 0.0034 0.0097 0.005 0.0052 0.004 0.0005 0.0012 0.0102 0.0014 0.0024 0.003 0.0004 0.0035 0.0092 0.0028 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.1654 45.3273 45.4321 54.24 55.684 59.7536 55.8344 59.3256 49.2829 63.6315
Translational Efficiency 0.7227 0.8903 0.8506 0.6103 0.5841 0.6534 0.6668 0.479 0.5723 0.5363

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Nam7

Nam7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nam7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available