Standard name
Human Ortholog
Description Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.06 0.13 0.16 0.11 0 0 0 0 0 0 0 0.1 0.17 0.17 0.1 0.14 0.11 0.13 0.1 0.14 0.14 0.14 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.08 0.08 0.07
Mitochondria 0.63 0.48 0.32 0.49 0.33 0.62 0.72 0.63 0.84 0.79 0.86 0.85 0.43 0.26 0.18 0.18 0.06 0.16 0.44 0.41 0.3 0.29 0.19 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.06 0.07 0.14 0.16 0.2 0.19 0.21 0.17 0.23 0.2 0.18 0.12 0.15 0.18 0.15 0.1 0.13 0 0 0 0 0 0
Peroxisomes 0.14 0.3 0.37 0.36 0.19 0.12 0.08 0.07 0 0.05 0 0 0.4 0.33 0.3 0.28 0.42 0.54 0.11 0.11 0.18 0.17 0.15 0.17
SpindlePole 0.16 0.21 0.43 0.28 0.2 0.12 0.05 0.1 0 0 0 0 0.34 0.4 0.45 0.34 0.49 0.27 0.12 0.13 0.21 0.17 0.24 0.25
Vac/Vac Membrane 0 0.07 0 0 0.11 0.07 0.05 0 0 0.05 0 0.05 0.05 0 0.06 0.11 0 0.06 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 1 0 2 4 7 2 3 3 2
Bud 10 1 8 2 11 12 9 3 7 8 7 3 2 0 2 3 1 2 5 3 5 3 3 5
Bud Neck 5 2 7 4 6 2 4 3 1 1 2 1 1 2 1 1 4 0 2 4 1 0 3 3
Bud Site 5 6 1 3 3 1 9 3 2 3 2 1 0 0 0 0 6 4
Cell Periphery 1 1 2 0 0 0 0 1 0 0 1 1 0 0 1 1 1 1 0 0 0 0 0 0
Cytoplasm 10 1 6 3 6 4 5 3 3 2 5 3 3 4 11 11 8 4 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Endosome 9 8 24 29 23 5 1 7 0 3 0 2 15 24 28 11 18 13 30 16 30 17 22 29
Golgi 0 0 0 1 5 0 0 0 0 0 0 0 1 6 1 4 1 0 13 5 6 9 11 13
Mitochondria 141 60 61 86 69 116 190 139 166 162 228 208 64 38 29 20 7 18 101 65 63 36 28 34
Nucleus 0 1 1 0 4 3 3 5 1 0 4 3 0 2 0 0 1 1 0 0 0 0 1 1
Nuclear Periphery 4 7 0 0 4 2 5 4 3 5 9 3 0 1 2 0 0 0 1 2 0 0 1 1
Nucleolus 44 7 13 25 33 37 51 46 33 46 54 45 18 22 29 16 12 15 3 4 4 1 5 6
Peroxisomes 32 37 70 64 40 22 21 15 6 10 6 4 59 48 50 31 53 61 25 17 38 21 23 33
SpindlePole 35 26 81 49 41 23 13 23 3 6 2 1 51 58 75 37 62 31 27 21 44 20 37 50
Vac/Vac Membrane 9 9 5 3 23 13 12 7 4 11 6 13 8 6 10 12 5 7 4 5 1 3 7 6
Unique Cell Count 225 124 190 176 208 186 263 220 197 204 265 244 148 144 165 110 126 114 232 160 213 125 155 199
Labelled Cell Count 305 166 279 270 268 240 323 259 229 258 326 288 223 212 239 149 180 159 232 160 213 125 155 199


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 11.9 9.3 11.0 13.5 13.7 13.3 15.6 15.1 15.7 16.4 18.1 12.2 12.3 12.1 15.2 13.9 14.5 10.1 10.3 10.7
Std Deviation (1e-4) 2.7 6.9 2.7 3.2 3.7 5.9 5.0 6.4 4.6 5.1 5.8 6.1 4.1 4.0 4.1 8.0 8.3 6.1 3.0 3.3 3.1
Intensity Change (Log2) 0.25 0.53 0.56 0.52 0.75 0.7 0.75 0.82 0.96 0.4 0.4 0.38 0.71 0.58 0.64 0.13 0.16 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.5 1.0 -0.4 -1.8 -0.3 -0.1 -0.9 0 0 0 0 0 0 0
Bud Neck -0.8 -0.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.2 0 0 0 0 0 0 0 0 0 1.5 2.5 1.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.0 -0.5 -3.6 -5.6 -3.6 -5.2 -4.4 -5.9 -5.1 -0.7 1.0 1.2 -0.7 0.4 -0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 0.2 5.9 8.5 6.3 10.4 9.5 11.8 11.3 2.1 -1.1 -3.1 -2.6 -5.6 -3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 2.8 3.7 3.8 4.0 3.0 4.4 4.0 3.5 1.7 2.5 3.1 2.2 0.9 1.8
Peroxisomes -0.1 -3.9 -5.6 -7.6 -7.5 -8.4 -7.9 -9.8 -9.7 0.6 -0.7 -1.3 -1.5 0.9 2.8
SpindlePole -3.0 -5.0 -6.6 -9.8 -7.5 -9.8 -9.5 -11.4 -11.1 -1.5 -0.4 0.5 -1.5 1.1 -2.7
Vacuole 0 3.3 2.0 1.1 0.3 0 1.4 0 1.4 1.3 0 1.6 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.9896 40.0583 33.6714 33.2175 25.177 35.5671 21.4198 31.1015 28.4545 27.0856 23.4212 28.2338 22.4521 37.3718 31.3654 27.0852 22.1416 33.0082
Actin 0.0024 0.0025 0.002 0.0022 0.0019 0.0044 0.022 0.0029 0.0108 0.0007 0.0011 0.0039 0.0214 0.0039 0.009 0.0003 0.0053 0.0105
Bud 0 0.0001 0 0 0.0003 0 0.0005 0.0003 0.0002 0 0 0 0.0009 0.0002 0.0003 0 0.0004 0.0003
Bud Neck 0 0.0001 0.0001 0 0.0002 0.0011 0.0002 0.0003 0.0001 0 0.0001 0.0001 0.0004 0.0004 0.0004 0 0.0005 0.0003
Bud Periphery 0 0.0001 0.0001 0.0001 0.0004 0 0.001 0.0004 0.0002 0.0001 0.0001 0 0.0011 0.0002 0.0006 0 0.001 0.0007
Bud Site 0.0002 0.0003 0.0005 0.0002 0.0004 0.0001 0.0014 0.0013 0.0006 0 0.0001 0.0001 0.0029 0.0026 0.0026 0 0.0007 0.0005
Cell Periphery 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0.0003 0.0008 0.0002 0.0007 0.0001 0.0001 0.0011 0.0006 0.0006 0.0001 0.0004 0.0006
Cytoplasm 0.0008 0 0 0.0017 0.0001 0 0.0039 0 0 0 0 0 0.0024 0 0.0003 0 0.0021 0.0259
Cytoplasmic Foci 0.0127 0.0094 0.0107 0.0067 0.0104 0.0196 0.0449 0.0324 0.0507 0.0046 0.0358 0.0445 0.0351 0.0378 0.049 0.0343 0.0235 0.0057
Eisosomes 0.0006 0.0003 0.0003 0.0003 0.0001 0.0004 0.0005 0.0004 0.0005 0.0002 0.0002 0.0005 0.0006 0.0004 0.0006 0.0001 0.0003 0.0005
Endoplasmic Reticulum 0.0003 0 0 0.0008 0.0001 0 0.0005 0.0001 0 0 0 0 0.0018 0 0.0008 0 0.0029 0.0017
Endosome 0.0074 0.0008 0.0022 0.0074 0.0146 0.0003 0.0047 0.0028 0.0009 0.0004 0.0036 0.0007 0.0113 0.0012 0.0067 0.0005 0.0371 0.0108
Golgi 0.0117 0.0025 0.0042 0.0032 0.0232 0.0045 0.0074 0.0155 0.0028 0.0014 0.0216 0.0082 0.0045 0.0086 0.0031 0.0027 0.0187 0.0081
Lipid Particles 0.0361 0.043 0.0377 0.0579 0.0168 0.0155 0.0516 0.0572 0.0375 0.0106 0.1649 0.0837 0.0359 0.0583 0.0439 0.0074 0.0374 0.0252
Mitochondria 0.8713 0.8915 0.898 0.8415 0.9035 0.9132 0.7874 0.7546 0.8089 0.934 0.7289 0.8137 0.7359 0.7846 0.7884 0.9082 0.6983 0.8435
None 0.0008 0 0 0.005 0.0001 0 0.0006 0.0001 0 0 0 0 0.0022 0 0.0004 0 0.0013 0.0004
Nuclear Periphery 0.0016 0 0 0.0007 0.0001 0 0.0025 0.0038 0.0001 0 0 0 0.0172 0 0.0009 0 0.0705 0.0097
Nucleolus 0.0118 0.0036 0.0008 0.0014 0.0011 0.0016 0.0037 0.0122 0.0061 0.0024 0.0034 0.0014 0.0211 0.013 0.0274 0.0002 0.0035 0.0019
Nucleus 0.0017 0.0001 0.0001 0.0007 0.0001 0 0.0093 0.0016 0.0003 0.0002 0.0001 0.0001 0.0135 0.0005 0.0015 0 0.0186 0.0022
Peroxisomes 0.0311 0.0373 0.0371 0.0282 0.0155 0.0364 0.047 0.0773 0.0621 0.0142 0.0373 0.0394 0.0558 0.0563 0.0462 0.0392 0.0567 0.015
Punctate Nuclear 0.0028 0 0.0001 0.0011 0.0001 0 0.0006 0.0002 0.0001 0 0.0005 0.0001 0.01 0.0001 0.0004 0 0.0045 0.0007
Vacuole 0.0009 0.0011 0.0005 0.0046 0.0028 0.0002 0.0032 0.0046 0.0026 0.0062 0.0004 0.0003 0.008 0.0058 0.0038 0.0003 0.0062 0.0058
Vacuole Periphery 0.0054 0.0072 0.0055 0.0359 0.0082 0.0027 0.0068 0.0311 0.0152 0.0241 0.0017 0.0032 0.0168 0.0256 0.0131 0.0065 0.0101 0.03

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 416.2222 355.8999 357.5314 429.5683 452.1394 392.0392 520.1027 558.8501 554.4613 593.1776
Translational Efficiency 1.5376 1.8824 1.5326 1.3517 1.4258 2.1194 1.7534 1.5015 1.5777 1.53

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
Localization
Cell Percentages mitochondrion (84%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Adh3

Adh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available