Standard name | |
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Human Ortholog | |
Description | Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 4 | 7 | 2 | 3 | 3 | 2 |
Bud | 10 | 1 | 8 | 2 | 11 | 12 | 9 | 3 | 7 | 8 | 7 | 3 | 2 | 0 | 2 | 3 | 1 | 2 | 5 | 3 | 5 | 3 | 3 | 5 |
Bud Neck | 5 | 2 | 7 | 4 | 6 | 2 | 4 | 3 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 1 | 4 | 0 | 2 | 4 | 1 | 0 | 3 | 3 |
Bud Site | 5 | 6 | 1 | 3 | 3 | 1 | 9 | 3 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 6 | 4 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 1 | 6 | 3 | 6 | 4 | 5 | 3 | 3 | 2 | 5 | 3 | 3 | 4 | 11 | 11 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 9 | 8 | 24 | 29 | 23 | 5 | 1 | 7 | 0 | 3 | 0 | 2 | 15 | 24 | 28 | 11 | 18 | 13 | 30 | 16 | 30 | 17 | 22 | 29 |
Golgi | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 4 | 1 | 0 | 13 | 5 | 6 | 9 | 11 | 13 |
Mitochondria | 141 | 60 | 61 | 86 | 69 | 116 | 190 | 139 | 166 | 162 | 228 | 208 | 64 | 38 | 29 | 20 | 7 | 18 | 101 | 65 | 63 | 36 | 28 | 34 |
Nucleus | 0 | 1 | 1 | 0 | 4 | 3 | 3 | 5 | 1 | 0 | 4 | 3 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 4 | 7 | 0 | 0 | 4 | 2 | 5 | 4 | 3 | 5 | 9 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 |
Nucleolus | 44 | 7 | 13 | 25 | 33 | 37 | 51 | 46 | 33 | 46 | 54 | 45 | 18 | 22 | 29 | 16 | 12 | 15 | 3 | 4 | 4 | 1 | 5 | 6 |
Peroxisomes | 32 | 37 | 70 | 64 | 40 | 22 | 21 | 15 | 6 | 10 | 6 | 4 | 59 | 48 | 50 | 31 | 53 | 61 | 25 | 17 | 38 | 21 | 23 | 33 |
SpindlePole | 35 | 26 | 81 | 49 | 41 | 23 | 13 | 23 | 3 | 6 | 2 | 1 | 51 | 58 | 75 | 37 | 62 | 31 | 27 | 21 | 44 | 20 | 37 | 50 |
Vac/Vac Membrane | 9 | 9 | 5 | 3 | 23 | 13 | 12 | 7 | 4 | 11 | 6 | 13 | 8 | 6 | 10 | 12 | 5 | 7 | 4 | 5 | 1 | 3 | 7 | 6 |
Unique Cell Count | 225 | 124 | 190 | 176 | 208 | 186 | 263 | 220 | 197 | 204 | 265 | 244 | 148 | 144 | 165 | 110 | 126 | 114 | 232 | 160 | 213 | 125 | 155 | 199 |
Labelled Cell Count | 305 | 166 | 279 | 270 | 268 | 240 | 323 | 259 | 229 | 258 | 326 | 288 | 223 | 212 | 239 | 149 | 180 | 159 | 232 | 160 | 213 | 125 | 155 | 199 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 11.9 | 9.3 | 11.0 | 13.5 | 13.7 | 13.3 | 15.6 | 15.1 | 15.7 | 16.4 | 18.1 | 12.2 | 12.3 | 12.1 | 15.2 | 13.9 | 14.5 | 10.1 | 10.3 | 10.7 |
Std Deviation (1e-4) | 2.7 | 6.9 | 2.7 | 3.2 | 3.7 | 5.9 | 5.0 | 6.4 | 4.6 | 5.1 | 5.8 | 6.1 | 4.1 | 4.0 | 4.1 | 8.0 | 8.3 | 6.1 | 3.0 | 3.3 | 3.1 |
Intensity Change (Log2) | – | – | – | 0.25 | 0.53 | 0.56 | 0.52 | 0.75 | 0.7 | 0.75 | 0.82 | 0.96 | 0.4 | 0.4 | 0.38 | 0.71 | 0.58 | 0.64 | 0.13 | 0.16 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0.5 | 1.0 | -0.4 | -1.8 | -0.3 | -0.1 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -0.8 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 2.5 | 1.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.0 | -0.5 | -3.6 | -5.6 | -3.6 | -5.2 | -4.4 | -5.9 | -5.1 | -0.7 | 1.0 | 1.2 | -0.7 | 0.4 | -0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 0.2 | 5.9 | 8.5 | 6.3 | 10.4 | 9.5 | 11.8 | 11.3 | 2.1 | -1.1 | -3.1 | -2.6 | -5.6 | -3.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.3 | 2.8 | 3.7 | 3.8 | 4.0 | 3.0 | 4.4 | 4.0 | 3.5 | 1.7 | 2.5 | 3.1 | 2.2 | 0.9 | 1.8 |
Peroxisomes | -0.1 | -3.9 | -5.6 | -7.6 | -7.5 | -8.4 | -7.9 | -9.8 | -9.7 | 0.6 | -0.7 | -1.3 | -1.5 | 0.9 | 2.8 |
SpindlePole | -3.0 | -5.0 | -6.6 | -9.8 | -7.5 | -9.8 | -9.5 | -11.4 | -11.1 | -1.5 | -0.4 | 0.5 | -1.5 | 1.1 | -2.7 |
Vacuole | 0 | 3.3 | 2.0 | 1.1 | 0.3 | 0 | 1.4 | 0 | 1.4 | 1.3 | 0 | 1.6 | 3.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 32.9896 | 40.0583 | 33.6714 | 33.2175 | 25.177 | 35.5671 | 21.4198 | 31.1015 | 28.4545 | 27.0856 | 23.4212 | 28.2338 | 22.4521 | 37.3718 | 31.3654 | 27.0852 | 22.1416 | 33.0082 |
Actin | 0.0024 | 0.0025 | 0.002 | 0.0022 | 0.0019 | 0.0044 | 0.022 | 0.0029 | 0.0108 | 0.0007 | 0.0011 | 0.0039 | 0.0214 | 0.0039 | 0.009 | 0.0003 | 0.0053 | 0.0105 |
Bud | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0005 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0009 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0003 |
Bud Neck | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0004 | 0 | 0.0005 | 0.0003 |
Bud Periphery | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0011 | 0.0002 | 0.0006 | 0 | 0.001 | 0.0007 |
Bud Site | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0014 | 0.0013 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0029 | 0.0026 | 0.0026 | 0 | 0.0007 | 0.0005 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0011 | 0.0006 | 0.0006 | 0.0001 | 0.0004 | 0.0006 |
Cytoplasm | 0.0008 | 0 | 0 | 0.0017 | 0.0001 | 0 | 0.0039 | 0 | 0 | 0 | 0 | 0 | 0.0024 | 0 | 0.0003 | 0 | 0.0021 | 0.0259 |
Cytoplasmic Foci | 0.0127 | 0.0094 | 0.0107 | 0.0067 | 0.0104 | 0.0196 | 0.0449 | 0.0324 | 0.0507 | 0.0046 | 0.0358 | 0.0445 | 0.0351 | 0.0378 | 0.049 | 0.0343 | 0.0235 | 0.0057 |
Eisosomes | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0005 |
Endoplasmic Reticulum | 0.0003 | 0 | 0 | 0.0008 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0018 | 0 | 0.0008 | 0 | 0.0029 | 0.0017 |
Endosome | 0.0074 | 0.0008 | 0.0022 | 0.0074 | 0.0146 | 0.0003 | 0.0047 | 0.0028 | 0.0009 | 0.0004 | 0.0036 | 0.0007 | 0.0113 | 0.0012 | 0.0067 | 0.0005 | 0.0371 | 0.0108 |
Golgi | 0.0117 | 0.0025 | 0.0042 | 0.0032 | 0.0232 | 0.0045 | 0.0074 | 0.0155 | 0.0028 | 0.0014 | 0.0216 | 0.0082 | 0.0045 | 0.0086 | 0.0031 | 0.0027 | 0.0187 | 0.0081 |
Lipid Particles | 0.0361 | 0.043 | 0.0377 | 0.0579 | 0.0168 | 0.0155 | 0.0516 | 0.0572 | 0.0375 | 0.0106 | 0.1649 | 0.0837 | 0.0359 | 0.0583 | 0.0439 | 0.0074 | 0.0374 | 0.0252 |
Mitochondria | 0.8713 | 0.8915 | 0.898 | 0.8415 | 0.9035 | 0.9132 | 0.7874 | 0.7546 | 0.8089 | 0.934 | 0.7289 | 0.8137 | 0.7359 | 0.7846 | 0.7884 | 0.9082 | 0.6983 | 0.8435 |
None | 0.0008 | 0 | 0 | 0.005 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0.0022 | 0 | 0.0004 | 0 | 0.0013 | 0.0004 |
Nuclear Periphery | 0.0016 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0025 | 0.0038 | 0.0001 | 0 | 0 | 0 | 0.0172 | 0 | 0.0009 | 0 | 0.0705 | 0.0097 |
Nucleolus | 0.0118 | 0.0036 | 0.0008 | 0.0014 | 0.0011 | 0.0016 | 0.0037 | 0.0122 | 0.0061 | 0.0024 | 0.0034 | 0.0014 | 0.0211 | 0.013 | 0.0274 | 0.0002 | 0.0035 | 0.0019 |
Nucleus | 0.0017 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0093 | 0.0016 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0135 | 0.0005 | 0.0015 | 0 | 0.0186 | 0.0022 |
Peroxisomes | 0.0311 | 0.0373 | 0.0371 | 0.0282 | 0.0155 | 0.0364 | 0.047 | 0.0773 | 0.0621 | 0.0142 | 0.0373 | 0.0394 | 0.0558 | 0.0563 | 0.0462 | 0.0392 | 0.0567 | 0.015 |
Punctate Nuclear | 0.0028 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.01 | 0.0001 | 0.0004 | 0 | 0.0045 | 0.0007 |
Vacuole | 0.0009 | 0.0011 | 0.0005 | 0.0046 | 0.0028 | 0.0002 | 0.0032 | 0.0046 | 0.0026 | 0.0062 | 0.0004 | 0.0003 | 0.008 | 0.0058 | 0.0038 | 0.0003 | 0.0062 | 0.0058 |
Vacuole Periphery | 0.0054 | 0.0072 | 0.0055 | 0.0359 | 0.0082 | 0.0027 | 0.0068 | 0.0311 | 0.0152 | 0.0241 | 0.0017 | 0.0032 | 0.0168 | 0.0256 | 0.0131 | 0.0065 | 0.0101 | 0.03 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 416.2222 | 355.8999 | 357.5314 | 429.5683 | 452.1394 | 392.0392 | 520.1027 | 558.8501 | 554.4613 | 593.1776 |
Translational Efficiency | 1.5376 | 1.8824 | 1.5326 | 1.3517 | 1.4258 | 2.1194 | 1.7534 | 1.5015 | 1.5777 | 1.53 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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