Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.15 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.08 0 0 0 0 0 0 0 0 0 0 0 0.34 0.32 0.6 0.27 0.33 0.4 0.07 0.09 0.05 0.07 0.08 0.1
Mitochondria 0.89 0.9 0.88 0.93 0.81 0.89 0.89 0.85 0.93 0.93 0.94 0.91 0.78 0.77 0.55 0.07 0.07 0.05 0.85 0.75 0.78 0.72 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.08 0.06 0.1 0.08 0.13 0.09 0.13 0.12 0.09 0.12 0 0 0 0.09 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0.08 0.15 0.11 0.11 0.12 0.11 0.08 0.06 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0.09 0.09 0.08 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.1 0.07 0.06 0.1 0.05 0 0 0 0 0 0 0.27 0.28 0.23 0.17 0.17 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 2 0 0 0 2 1 1 0 2 1 0 5 2 0 1 9 11 10 8 11 15
Bud 3 5 4 5 2 4 7 19 12 10 10 7 1 1 1 9 5 4 1 1 0 2 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 1 1 3 0 2 4 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 12 13 5 3 0 1 3 4 4 6 2 3 3 8 26 17 13 0 2 1 0 0 0
Cytoplasm 1 4 7 6 12 7 8 16 3 7 6 5 3 3 6 37 14 17 0 1 0 0 2 0
Endoplasmic Reticulum 1 4 2 2 4 5 2 4 3 3 9 23 1 1 6 7 8 10 1 1 3 0 2 1
Endosome 5 0 9 4 12 3 5 7 0 1 0 0 2 2 1 6 2 3 7 5 8 4 9 7
Golgi 43 13 16 6 8 0 1 2 1 2 2 0 41 39 135 57 38 49 38 29 21 15 25 40
Mitochondria 485 279 362 251 274 406 616 599 528 467 487 477 93 93 124 14 8 6 501 246 338 162 228 289
Nucleus 2 1 0 0 0 1 2 3 2 3 0 1 1 1 4 2 1 2 1 0 0 1 1 0
Nuclear Periphery 23 14 33 17 33 38 88 63 75 62 49 62 4 4 5 20 5 5 4 4 3 0 2 2
Nucleolus 24 12 16 13 26 67 76 80 66 54 40 31 0 0 1 7 6 8 2 4 0 0 1 5
Peroxisomes 6 1 29 8 11 3 15 12 5 1 0 5 2 2 4 14 7 8 5 3 40 19 27 35
SpindlePole 0 0 2 0 2 2 2 6 3 3 2 3 0 0 1 2 1 1 0 0 0 0 1 0
Vac/Vac Membrane 96 31 27 15 34 25 20 23 17 19 13 12 32 34 53 37 20 19 12 13 3 4 8 12
Unique Cell Count 548 311 413 270 338 455 691 701 566 501 516 523 120 121 226 212 116 122 590 328 434 225 329 418
Labelled Cell Count 701 378 521 334 422 562 846 839 722 641 628 633 184 183 354 240 132 146 590 328 434 225 329 418


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 13.1 10.3 10.5 10.8 9.2 9.3 9.4 9.1 8.5 8.8 9.0 12.6 12.6 12.2 8.9 9.7 11.6 12.6 13.4 14.5
Std Deviation (1e-4) 2.5 2.6 2.1 2.5 2.6 2.4 2.4 2.5 2.5 2.5 2.5 3.2 2.3 2.3 1.9 1.8 2.2 2.8 2.7 2.9 3.0
Intensity Change (Log2) – – – 0.04 0.07 -0.16 -0.14 -0.12 -0.17 -0.28 -0.23 -0.2 0.3 0.3 0.24 -0.2 -0.08 0.18 0.29 0.38 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2.0 1.4 1.3 1.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -2.1 -3.8 -4.3 -3.7 -2.9 -2.6 -2.1 -3.3 0 0 0.3 4.5 4.7 3.4
Cytoplasm 0.5 1.6 -0.2 -0.7 0.7 0 -0.4 -0.7 -1.0 0 0 0 7.3 0 5.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0 0
Endosome -0.7 1.1 -1.9 -2.1 -1.6 0 0 0 0 0 0 0 0.5 0 0
Golgi -1.2 -1.2 -4.2 -4.9 -4.6 -4.4 -3.8 -3.8 -4.5 9.5 9.0 15.9 8.5 9.1 10.8
Mitochondria 2.2 -2.5 0.7 0.8 -1.0 3.0 2.9 3.6 1.8 -2.8 -2.9 -9.3 -19.6 -16.8 -17.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.8 0.9 0.2 2.4 0.6 2.6 2.2 0.8 1.9 -1.8 -1.8 -3.0 0.6 -1.4 -1.5
Nucleolus 0.6 2.3 5.4 4.1 4.3 4.3 3.9 2.5 1.4 0 0 -2.6 -0.4 0 1.3
Peroxisomes -2.3 -2.3 -5.0 -4.0 -4.5 -5.2 -5.8 -6.1 -4.9 -2.2 -2.2 -2.9 -0.2 -0.4 -0.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 1.8 -0.6 -2.9 -2.5 -2.6 -1.9 -3.0 -3.2 6.2 6.6 6.2 4.3 3.6 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.3596 11.647 10.636 9.6717 10.0265 10.3415 12.0329 13.2142 12.6553 10.5194 11.7284 12.7137 13.0923 13.0242 13.1891 12.8436 12.9268 13.0501
Actin 0.0094 0.0029 0.009 0.0013 0.0008 0.0024 0.0223 0.0097 0.0127 0.0009 0.0038 0.0074 0.0085 0.008 0.0037 0.0028 0.0003 0.0006
Bud 0.0005 0.0006 0.0015 0.0002 0 0 0.0017 0.0003 0.0003 0.0001 0.0004 0 0.0002 0.0003 0 0.0001 0 0
Bud Neck 0.0011 0.0001 0.0003 0 0 0.0001 0.0002 0.0002 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0 0
Bud Periphery 0.0012 0.0011 0.0019 0.0008 0.0001 0.0001 0.0029 0.0004 0.0006 0.0002 0.0006 0 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002
Bud Site 0.001 0.0022 0.0031 0.0001 0.0001 0 0.0017 0.0073 0.0012 0.0001 0.0006 0 0.0008 0.0009 0 0.0001 0 0
Cell Periphery 0.0006 0.0005 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0002 0.0001 0.0002 0 0 0.0002 0 0 0 0.0001
Cytoplasm 0.0009 0 0.0002 0 0.0005 0.0001 0.0011 0.0002 0.0006 0.0003 0.015 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0076 0.0012 0.0016 0.0003 0.0008 0.0017 0.0029 0.0038 0.003 0.0052 0.027 0.0005 0.0009 0.0034 0.0007 0.0005 0.0009 0.0002
Eisosomes 0.0004 0.0002 0.0002 0.0002 0.0001 0.0002 0.0004 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0002 0.0002 0 0.0004 0.0012 0.0008 0.0001 0.0012 0.0007 0.0017 0 0 0 0 0 0 0.0001
Endosome 0.0082 0.0065 0.0036 0.0074 0.007 0.0051 0.008 0.0117 0.009 0.0175 0.0254 0.0023 0.003 0.0086 0.0016 0.002 0.0023 0.0021
Golgi 0.0149 0.0204 0.0062 0.0183 0.0241 0.0185 0.0139 0.0254 0.0226 0.0092 0.0215 0.0131 0.0085 0.0245 0.0105 0.0096 0.0518 0.0058
Lipid Particles 0.0297 0.0122 0.0037 0.0004 0.0079 0.0026 0.0052 0.0125 0.0042 0.0129 0.0159 0.0055 0.0018 0.0143 0.0025 0.0005 0.0004 0.0062
Mitochondria 0.8781 0.9231 0.9524 0.9664 0.8756 0.9599 0.9162 0.9103 0.9335 0.9266 0.8545 0.9597 0.9682 0.9142 0.9762 0.9821 0.9286 0.975
None 0.0007 0 0.0007 0 0.0002 0.0001 0.0009 0 0.0006 0.0002 0.0017 0 0 0 0 0 0 0
Nuclear Periphery 0.0009 0.0004 0.0003 0 0.0203 0.0029 0.0031 0.0008 0.0005 0.0013 0.0061 0.0001 0 0.0001 0 0 0 0.0006
Nucleolus 0.0115 0.0006 0.0001 0 0.0045 0 0.0007 0.0002 0.0001 0.0002 0.0006 0.0001 0 0.0004 0 0 0 0
Nucleus 0.0029 0.0002 0.0002 0 0.0195 0.0002 0.0041 0.0001 0.0002 0.0002 0.0103 0 0 0.0001 0 0 0 0.0001
Peroxisomes 0.0026 0.0045 0.0086 0.0014 0.0018 0.0014 0.0058 0.0066 0.0049 0.0034 0.0066 0.0058 0.0063 0.0106 0.0033 0.0011 0.0146 0.001
Punctate Nuclear 0.002 0 0.0003 0 0.0054 0.0001 0.0026 0 0.0004 0.0175 0.0025 0.0002 0 0 0 0 0 0
Vacuole 0.0105 0.0056 0.0016 0.0004 0.008 0.0004 0.0027 0.0022 0.0011 0.0014 0.0021 0.0007 0.0002 0.0051 0.0002 0.0002 0.0002 0.001
Vacuole Periphery 0.0144 0.0174 0.0042 0.0025 0.0227 0.0028 0.0023 0.008 0.0026 0.002 0.0031 0.0042 0.001 0.0084 0.0009 0.0006 0.0006 0.0067

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.8601 40.9995 46.9643 47.1853 41.5674 60.5246 52.3213 55.958 63.6657 57.1947
Translational Efficiency 0.8454 0.6256 0.605 0.692 0.5711 0.5796 0.555 0.555 0.4614 0.4931

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Yta12

Yta12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yta12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available