ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 1 | 9 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 4 | 0 | 8 | – | 5 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
Cytoplasm | 151 | 113 | 37 | 46 | 197 | 82 | 158 | – | 75 | 106 | 102 | 163 | 134 | 223 | 239 | 147 | 125 | 109 | 135 | 110 | 54 | 137 | 200 | 173 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 5 | 2 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 1 | 1 | 2 | 2 |
Golgi | 1 | 1 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 6 | 8 | 0 | 0 | 0 | 11 | 13 | – | 45 | 48 | 72 | 72 | 1 | 0 | 0 | 3 | 4 | 1 | 16 | 6 | 8 | 12 | 12 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 2 | – | 4 | 4 | 4 | 1 | 0 | 1 | 0 | 1 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 5 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 |
Vac/Vac Membrane | 3 | 0 | 0 | 0 | 0 | 0 | 4 | – | 1 | 4 | 2 | 3 | 0 | 0 | 0 | 19 | 11 | 9 | 4 | 1 | 0 | 1 | 2 | 1 |
Unique Cell Count | 160 | 119 | 37 | 47 | 198 | 87 | 168 | 103 | 134 | 157 | 204 | 134 | 223 | 239 | 164 | 132 | 118 | 175 | 127 | 71 | 161 | 235 | 205 | |
Labelled Cell Count | 164 | 124 | 37 | 47 | 204 | 96 | 191 | 135 | 169 | 196 | 247 | 136 | 225 | 239 | 182 | 159 | 128 | 175 | 127 | 71 | 161 | 235 | 205 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.1 | 3.9 | 4.4 | 5.0 | 3.7 | 4.4 | – | 3.7 | 3.7 | 3.9 | 3.7 | 4.6 | 4.9 | 5.3 | 8.2 | 7.7 | 7.8 | 4.7 | 4.9 | 5.0 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.3 | 1.4 | 1.6 | 1.7 | 1.8 | – | 1.7 | 1.5 | 1.9 | 1.1 | 1.1 | 1.6 | 1.7 | 2.3 | 1.9 | 2.0 | 3.9 | 3.5 | 3.4 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.36 | -0.05 | 0.2 | – | -0.08 | -0.05 | 0.03 | -0.06 | 0.24 | 0.34 | 0.45 | 1.08 | 1.0 | 1.01 | 0.29 | 0.36 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2447 | 0.216 | -0.0558 | 0.211 | -0.2363 | -0.1108 | -0.3676 | 0.7993 | 0.7039 | 0.5902 | 1.2126 | 0.5975 | 2.0308 | 3.2522 | 2.4612 | 2.5917 | 2.5229 | 2.802 |
Actin | 0.0369 | 0.0003 | 0.0187 | 0.0364 | 0.0354 | 0.001 | 0.0002 | 0.0035 | 0.0102 | 0.0001 | 0.0031 | 0.0019 | 0.0173 | 0.0013 | 0.0004 | 0.0004 | 0.0004 | 0.0002 |
Bud | 0.0005 | 0.0001 | 0.0006 | 0.001 | 0.0005 | 0.0001 | 0.0054 | 0.0074 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0 | 0 |
Bud Neck | 0.0153 | 0.0002 | 0.0005 | 0.0188 | 0.0005 | 0.0015 | 0 | 0.0001 | 0.0022 | 0.0002 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0007 | 0.0001 | 0.0004 | 0.0013 | 0.0007 | 0 | 0.0026 | 0.0019 | 0.0012 | 0.0001 | 0.0004 | 0.0009 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 |
Bud Site | 0.0046 | 0.0009 | 0.0019 | 0.004 | 0.0009 | 0.0001 | 0.001 | 0.0025 | 0.0042 | 0.0004 | 0.0036 | 0.0001 | 0.0004 | 0.0083 | 0.0003 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.3476 | 0.5523 | 0.4246 | 0.5235 | 0.5946 | 0.6069 | 0.2451 | 0.3819 | 0.3693 | 0.4068 | 0.5243 | 0.4982 | 0.4197 | 0.525 | 0.5216 | 0.4764 | 0.5279 | 0.6132 |
Cytoplasmic Foci | 0.0302 | 0.0136 | 0.0118 | 0.0319 | 0.0192 | 0.0159 | 0.0077 | 0.0068 | 0.009 | 0.0077 | 0.0188 | 0.0054 | 0.0095 | 0.0032 | 0.0046 | 0.0062 | 0.0024 | 0.0045 |
Eisosomes | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0058 | 0.0037 | 0.0031 | 0.0048 | 0.0036 | 0.0022 | 0.0014 | 0.0013 | 0.0013 | 0.0009 | 0.0005 | 0.0011 | 0.0022 | 0.0008 | 0.0015 | 0.003 | 0.0024 | 0.0012 |
Endosome | 0.0224 | 0.0045 | 0.0044 | 0.0144 | 0.0194 | 0.006 | 0.0011 | 0.0014 | 0.0064 | 0.031 | 0.0232 | 0.0034 | 0.0024 | 0.0004 | 0.0014 | 0.0017 | 0.0005 | 0.0011 |
Golgi | 0.0079 | 0.0003 | 0.002 | 0.0117 | 0.0288 | 0.0005 | 0.0001 | 0.0005 | 0.0031 | 0.0013 | 0.0431 | 0.0032 | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0085 | 0.0009 | 0.0053 | 0.0136 | 0.0275 | 0.0017 | 0.0006 | 0.0018 | 0.0035 | 0.0051 | 0.0331 | 0.0008 | 0.0012 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Mitochondria | 0.0049 | 0.0002 | 0.0008 | 0.0081 | 0.0078 | 0.0003 | 0.0014 | 0.0005 | 0.0012 | 0.0003 | 0.0754 | 0.0533 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
None | 0.4731 | 0.4174 | 0.4958 | 0.2885 | 0.2352 | 0.3413 | 0.7298 | 0.5875 | 0.5783 | 0.5341 | 0.2483 | 0.4262 | 0.5405 | 0.4548 | 0.4645 | 0.5064 | 0.4641 | 0.3759 |
Nuclear Periphery | 0.013 | 0.001 | 0.0061 | 0.0023 | 0.0025 | 0.0071 | 0.0004 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0004 | 0.0003 |
Nucleolus | 0.0013 | 0.0001 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0029 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0084 | 0.0019 | 0.0091 | 0.005 | 0.002 | 0.0094 | 0.0008 | 0.0006 | 0.0022 | 0.0008 | 0.0013 | 0.0009 | 0.0011 | 0.0016 | 0.0025 | 0.0016 | 0.0009 | 0.0025 |
Peroxisomes | 0.0096 | 0.0001 | 0.0087 | 0.0254 | 0.0103 | 0.0004 | 0.0003 | 0.0004 | 0.0045 | 0.0007 | 0.0161 | 0.0019 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 |
Punctate Nuclear | 0.0024 | 0.0008 | 0.0035 | 0.0064 | 0.0085 | 0.0037 | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0013 | 0.0003 | 0.0006 | 0.0005 | 0.0009 | 0.001 | 0.0003 | 0.0003 |
Vacuole | 0.0047 | 0.0013 | 0.0012 | 0.0018 | 0.0011 | 0.0013 | 0.001 | 0.0006 | 0.0009 | 0.0082 | 0.002 | 0.0007 | 0.0007 | 0.0005 | 0.0006 | 0.0009 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.3251 | 24.5152 | 16.0512 | 17.319 | 15.6865 | 16.1227 | 21.8711 | 27.5549 | 31.849 | 20.8406 |
Translational Efficiency | 1.7374 | 0.8542 | 1.1079 | 1.392 | 1.1102 | 3.5055 | 1.5129 | 1.1727 | 1.3281 | 1.3844 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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