Standard name | |
---|---|
Human Ortholog | |
Description | Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 8 | 0 | 1 | 5 | 4 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 1 | 2 | 8 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2 | 1 | 7 | 18 | 22 | 10 | 13 | 23 | 28 | 19 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 5 | 3 | 4 | 9 | 5 | 18 | 19 | 7 | 7 | 7 | 9 | 28 | 39 | 62 | 6 | 9 | 6 | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 3 | 1 | 0 | 0 | 3 | 4 | 3 | 2 | 6 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 2 |
Mitochondria | 5 | 8 | 9 | 3 | 10 | 65 | 80 | 48 | 27 | 107 | 83 | 80 | 0 | 0 | 0 | 7 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 5 |
Nucleus | 99 | 249 | 627 | 367 | 357 | 573 | 621 | 436 | 377 | 570 | 452 | 473 | 316 | 288 | 275 | 127 | 70 | 41 | 89 | 255 | 534 | 205 | 303 | 212 |
Nuclear Periphery | 0 | 0 | 5 | 1 | 2 | 4 | 8 | 3 | 5 | 9 | 7 | 4 | 1 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 51 | 125 | 209 | 165 | 134 | 428 | 425 | 333 | 321 | 549 | 463 | 493 | 88 | 54 | 66 | 29 | 23 | 12 | 20 | 46 | 180 | 51 | 87 | 123 |
Peroxisomes | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 2 | 4 | 5 | 0 | 0 | 4 | 12 | 9 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 0 | 3 |
Vac/Vac Membrane | 0 | 3 | 2 | 1 | 2 | 3 | 4 | 4 | 0 | 1 | 3 | 1 | 1 | 4 | 3 | 2 | 4 | 1 | 3 | 6 | 8 | 6 | 17 | 11 |
Unique Cell Count | 117 | 303 | 715 | 435 | 432 | 823 | 902 | 688 | 581 | 913 | 762 | 794 | 372 | 366 | 369 | 150 | 95 | 57 | 117 | 320 | 742 | 274 | 415 | 375 |
Labelled Cell Count | 156 | 393 | 864 | 546 | 525 | 1101 | 1188 | 858 | 757 | 1284 | 1052 | 1089 | 440 | 405 | 420 | 179 | 111 | 63 | 117 | 320 | 742 | 274 | 415 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 12.3 | 9.6 | 9.1 | 8.9 | 7.7 | 8.0 | 8.0 | 7.9 | 7.5 | 7.3 | 7.2 | 11.3 | 11.4 | 10.8 | 11.4 | 10.3 | 11.7 | 12.0 | 12.8 | 13.5 |
Std Deviation (1e-4) | 1.8 | 4.0 | 2.1 | 2.4 | 2.5 | 2.2 | 2.5 | 2.1 | 2.2 | 2.2 | 2.4 | 2.5 | 3.0 | 3.2 | 3.4 | 3.6 | 2.8 | 3.6 | 2.7 | 2.8 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.12 | -0.31 | -0.26 | -0.26 | -0.28 | -0.36 | -0.39 | -0.41 | 0.23 | 0.25 | 0.17 | 0.25 | 0.1 | 0.29 | 0.32 | 0.42 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.3 | 3.6 | 2.4 | 3.3 | 3.6 | 4.6 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 2.8 | 3.5 | 0 | 0 | 0 | 1.6 | 6.7 | 8.2 | 10.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.4 | 6.1 | 6.7 | 5.4 | 3.7 | 8.1 | 7.7 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.6 | -2.4 | -8.5 | -9.0 | -10.6 | -9.8 | -11.5 | -12.3 | -12.3 | -1.3 | -3.9 | -5.5 | -1.0 | -3.7 | -3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0.4 | 0 | 0 | 0.6 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.1 | 0.6 | 9.0 | 7.3 | 7.4 | 9.5 | 12.4 | 12.2 | 12.8 | -2.0 | -5.2 | -4.1 | -2.5 | -1.0 | -1.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.8377 | 9.4954 | 8.7552 | 8.0454 | 7.7485 | 8.7844 | 8.5361 | 8.0504 | 7.2441 | 7.0005 | 6.9986 | 7.6468 | 9.6653 | 8.0936 | 7.9385 | 8.0217 | 6.8438 | 7.5129 |
Actin | 0.0272 | 0 | 0.0005 | 0 | 0.0012 | 0.0042 | 0.0352 | 0 | 0.0002 | 0.0037 | 0.004 | 0 | 0.0069 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.001 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0002 |
Bud Periphery | 0.0003 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0017 | 0 | 0.0036 | 0.0005 | 0.0005 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0103 | 0 | 0.0006 | 0 | 0.0007 | 0.0001 | 0.0149 | 0 | 0.0002 | 0.0132 | 0.0005 | 0 | 0.0021 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0015 | 0 | 0.0054 | 0 | 0.0406 | 0.0009 | 0.0141 | 0 | 0.0044 | 0.0096 | 0.0166 | 0 | 0.0079 | 0 | 0 | 0 | 0 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0022 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0074 | 0 | 0.0001 | 0.0011 | 0.0005 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0156 | 0 | 0.0017 | 0 | 0.0056 | 0.0011 | 0.0263 | 0 | 0.0013 | 0.0149 | 0.0189 | 0 | 0.0038 | 0 | 0 | 0 | 0 | 0.0001 |
Golgi | 0.0017 | 0 | 0.0005 | 0 | 0.0027 | 0.001 | 0.005 | 0 | 0.0005 | 0.0043 | 0.0135 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0018 | 0 | 0.0093 | 0 | 0.0382 | 0.0013 | 0.0102 | 0 | 0.0045 | 0.0156 | 0.0284 | 0 | 0.0056 | 0 | 0 | 0 | 0 | 0.0003 |
Mitochondria | 0.0021 | 0.0001 | 0.0104 | 0 | 0.0009 | 0.0003 | 0.0164 | 0.0001 | 0.0105 | 0.0061 | 0.0201 | 0.0001 | 0.0011 | 0 | 0 | 0.0001 | 0.0007 | 0.0008 |
None | 0.003 | 0 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0154 | 0 | 0.0002 | 0.002 | 0.001 | 0 | 0.0035 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0194 | 0.0013 | 0.0045 | 0.0001 | 0.0008 | 0.0001 | 0.0285 | 0.0005 | 0.0042 | 0.0027 | 0.0053 | 0.0002 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 |
Nucleolus | 0.0687 | 0.025 | 0.0427 | 0.0215 | 0.2468 | 0.1658 | 0.076 | 0.055 | 0.0568 | 0.0218 | 0.2731 | 0.1924 | 0.0389 | 0.0313 | 0.0309 | 0.0419 | 0.288 | 0.1499 |
Nucleus | 0.8339 | 0.9733 | 0.9085 | 0.9783 | 0.633 | 0.8223 | 0.704 | 0.9438 | 0.9067 | 0.8933 | 0.5649 | 0.806 | 0.91 | 0.9667 | 0.9689 | 0.9579 | 0.7098 | 0.8475 |
Peroxisomes | 0.0021 | 0 | 0.0006 | 0 | 0.0082 | 0.0015 | 0.0049 | 0 | 0.0025 | 0.0054 | 0.0209 | 0 | 0.0065 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0063 | 0.0001 | 0.0104 | 0.0001 | 0.0197 | 0.0002 | 0.0301 | 0.0004 | 0.0015 | 0.0043 | 0.0278 | 0.0002 | 0.0045 | 0.0018 | 0 | 0 | 0.0007 | 0.0003 |
Vacuole | 0.0012 | 0 | 0.0011 | 0 | 0.0002 | 0.0003 | 0.0031 | 0 | 0.0008 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0017 | 0 | 0.0018 | 0 | 0.0002 | 0.0001 | 0.0029 | 0 | 0.0007 | 0.0005 | 0.0017 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.1791 | 44.4925 | 44.207 | 45.2077 | 47.0308 | 30.3521 | 39.8396 | 36.2809 | 42.8463 | 44.0417 |
Translational Efficiency | 2.3175 | 2.189 | 1.8565 | 2.1861 | 1.9672 | 2.9339 | 1.8005 | 2.2703 | 1.7761 | 1.8602 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|