Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.11 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.09 0.13 0.1 0.12 0.18 0.18 0.13 0.22 0.24 0.17 0.24 0.1 0 0 0.09 0.05 0.1 0 0 0 0 0 0
Nucleus 0.19 0.21 0.13 0.17 0.1 0.08 0.07 0.07 0.07 0 0.06 0.09 0 0.06 0.11 0.1 0.13 0.1 0.12 0.18 0.09 0.1 0 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.88 0.92 0.91 0.87 0.86 0.87 0.87 0.8 0.87 0.86 0.82 0.9 0.85 0.81 0.87 0.88 0.91 0.77 0.74 0.74 0.7 0.76 0.68
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0 0.2 0.2 0.26 0.27 0.18 0.28 0.24 0.24 0 0.11 0.08 0.12 0.11 0.05 0.08 0 0.11 0.08 0.11 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 2 5 7 6 3 1 0 0 0 0 1 0 0 2 2 4 0 0 2
Bud Neck 1 1 3 0 1 4 3 5 1 1 1 5 3 0 0 1 4 1 0 0 0 0 0 2
Bud Site 2 0 1 1 1 9 6 10 7 8 8 9 1 1 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 3 3 3 1 4 4 1 4 4 2 2 3 7 15 18 26 14 11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 2 0 0 0 0 2 0
Endosome 0 2 0 2 6 10 5 15 7 1 4 3 1 2 2 11 5 2 0 6 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Mitochondria 34 32 27 15 26 54 48 60 40 56 27 54 12 6 4 62 19 35 0 1 1 0 1 1
Nucleus 41 70 27 26 20 26 19 32 12 9 9 19 2 9 18 69 50 37 24 63 18 5 5 7
Nuclear Periphery 2 1 0 1 1 2 2 0 1 1 0 2 0 0 0 0 3 1 1 0 0 0 0 0
Nucleolus 201 299 185 139 180 263 228 403 144 205 134 181 106 122 133 594 336 321 167 262 153 37 118 69
Peroxisomes 0 2 0 1 7 7 5 11 4 0 3 4 0 2 1 7 2 1 0 0 0 0 0 0
SpindlePole 8 11 20 6 42 62 67 125 33 66 38 54 5 16 13 81 44 19 18 10 22 4 17 9
Vac/Vac Membrane 0 4 1 0 1 0 1 4 0 0 0 1 0 0 1 7 3 4 0 2 2 1 6 5
Unique Cell Count 218 339 201 153 208 307 261 462 179 236 156 221 118 144 165 684 383 353 216 354 207 53 157 102
Labelled Cell Count 292 425 267 192 290 443 390 677 259 352 227 335 137 173 190 862 482 434 216 354 207 53 157 102


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 13.5 11.3 11.8 9.6 7.9 6.7 7.2 7.2 6.5 6.8 6.9 12.2 13.6 13.1 14.5 17.3 17.9 11.2 12.3 13.3
Std Deviation (1e-4) 2.7 4.4 3.4 3.4 3.1 2.9 2.0 1.9 2.1 1.9 2.0 2.4 4.0 6.5 5.1 4.9 5.6 5.8 2.7 2.7 3.1
Intensity Change (Log2) – – – 0.07 -0.23 -0.5 -0.75 -0.64 -0.66 -0.79 -0.72 -0.7 0.11 0.28 0.22 0.37 0.62 0.67 -0.01 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 3.7 3.9 1.6 1.5 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -0.3 1.3 1.4 -0.2 2.3 2.7 1.0 2.9 -0.9 -2.9 -3.8 -1.8 -3.6 -1.3
Nucleus 0.9 -1.2 -1.8 -2.2 -2.7 -2.2 -3.6 -2.4 -1.6 -3.5 -2.2 -0.7 -1.3 -0.1 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.4 -1.8 -2.2 -1.6 -1.8 -3.3 -1.7 -1.9 -3.1 -0.7 -2.1 -3.2 -2.0 -1.6 -0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.2 2.9 3.1 4.3 4.9 2.4 4.7 3.7 3.9 -1.8 0.3 -0.7 0.7 0.6 -2.0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.9015 14.004 12.1579 11.8228 11.0488 11.8594 15.9919 16.1759 14.1118 12.1048 13.6573 14.3158 12.4606 17.3829 15.5774 16.4049 15.0581 15.5261
Actin 0 0 0 0 0 0 0.0034 0 0.0014 0.0023 0 0.0002 0.0603 0 0.0421 0 0 0.004
Bud 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0 0.001 0.0001 0.0002 0.0001 0 0
Bud Neck 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0014 0.0001 0.0002 0.0002 0.0031 0.0001 0.0015 0.0001 0.0004 0.0003 0.0001 0.0001
Bud Periphery 0 0 0 0 0 0.0001 0.0002 0 0.0001 0.0002 0.0001 0 0.0015 0.0001 0.0002 0.0002 0 0
Bud Site 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0052 0 0.0006 0.0003 0.0003 0 0.0031 0.0002 0.0006 0.0002 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0004 0.0001 0.0001 0.001 0.0004 0.0002 0.0002 0.0001 0
Cytoplasm 0 0 0 0 0 0 0.002 0 0.0002 0.0001 0 0.0007 0.0507 0 0.0026 0 0.0002 0.0001
Cytoplasmic Foci 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0134 0 0.006 0.0068 0.0053 0.0029 0.0486 0.0003 0.0042 0.0004 0.0287 0.001
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0006 0 0.0002 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0013 0 0.0001 0.0003 0 0.0003 0.0066 0 0.0023 0 0 0.0001
Endosome 0 0 0 0 0 0 0.015 0 0.0036 0.019 0.0001 0.0024 0.0219 0.0001 0.015 0 0.0001 0.0008
Golgi 0 0 0 0 0 0 0.0039 0 0.0037 0.0212 0.0001 0.001 0.0046 0 0.0116 0 0 0.0005
Lipid Particles 0.0002 0 0.0006 0.0003 0.0002 0.0002 0.0181 0.0001 0.0062 0.0179 0.0044 0.0034 0.0114 0.0004 0.0051 0.0006 0.0026 0.0013
Mitochondria 0.0005 0.0003 0.0002 0.0001 0.0004 0.0001 0.0035 0.0001 0.0004 0.0238 0.0015 0.0006 0.0066 0.0021 0.0051 0.0003 0.0015 0.0001
None 0 0 0 0 0 0 0.0009 0 0.0001 0.0001 0.0001 0.0019 0.0205 0.0001 0.0011 0 0.0007 0.0003
Nuclear Periphery 0 0 0 0 0.0001 0 0.0052 0 0.0003 0.0005 0.0004 0.0012 0.0115 0.0001 0.0011 0.0001 0.0001 0.0008
Nucleolus 0.8793 0.8946 0.9219 0.9452 0.8694 0.9083 0.8253 0.862 0.8431 0.7894 0.8699 0.8911 0.5432 0.8689 0.7094 0.8572 0.8898 0.8527
Nucleus 0.1191 0.1043 0.074 0.0533 0.1289 0.0905 0.0854 0.1372 0.1307 0.1055 0.0993 0.0893 0.1013 0.1234 0.1908 0.138 0.0688 0.1312
Peroxisomes 0 0 0 0 0 0 0.0038 0 0.0016 0.0043 0.0003 0.0001 0.0041 0.0001 0.0048 0 0.0002 0.0009
Punctate Nuclear 0.0002 0.0001 0.002 0.0005 0.0004 0.0003 0.0081 0.0004 0.0011 0.0028 0.0143 0.0042 0.0823 0.0004 0.0022 0.0012 0.0065 0.0056
Vacuole 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0021 0.0001 0.0003 0.002 0.0002 0.0002 0.0102 0.0015 0.0006 0.0006 0.0002 0.0001
Vacuole Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.001 0 0.0001 0.0029 0.0002 0.0003 0.0072 0.0019 0.0005 0.0003 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.8786 37.101 34.6506 42.9118 37.5744 27.1765 32.0153 27.5013 26.774 43.5213
Translational Efficiency 0.8837 1.1762 1.2133 0.9137 1.1295 1.3322 1.0462 0.8886 0.8345 0.8043

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
Localization
Cell Percentages nucleolus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp15

Utp15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available