Standard name
Human Ortholog
Description Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.91 0.99 0.94 0.94 0.9 0.78 0.73 0.68 0.67 0.62 0.66 0.98 0.98 0.97 0.95 0.97 0.96 0.88 0.84 0.93 0.91 0.93 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.05 0.1 0.05 0.17 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.07 0.09 0.16 0.2 0.25 0.27 0.19 0.24 0 0 0 0.08 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 4 0 1 4 4 1 0 0 0 0 0 1 2 1 0 0 0
Bud 0 1 0 0 0 0 1 11 4 8 10 7 0 2 0 0 0 0 4 19 2 1 1 2
Bud Neck 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0
Bud Site 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 7 5 3 1 4 7 9 8 9 13 11 0 5 0 9 13 11 0 0 0 0 0 0
Cytoplasm 203 204 200 230 326 382 338 356 207 259 245 261 195 229 259 330 352 326 216 243 188 49 114 144
Endoplasmic Reticulum 0 2 0 0 0 0 5 4 3 4 11 9 1 0 0 9 8 11 1 1 1 0 0 1
Endosome 0 1 0 1 0 1 1 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0
Golgi 1 0 1 1 0 0 0 2 0 3 0 4 0 0 0 1 0 2 2 0 0 0 0 0
Mitochondria 4 0 0 1 1 15 22 25 29 19 68 30 0 0 0 5 2 0 4 1 2 0 0 1
Nucleus 6 8 3 1 1 1 6 12 5 6 8 6 0 0 0 2 6 3 0 1 0 1 1 2
Nuclear Periphery 3 5 0 0 0 1 2 0 1 4 3 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 0 0 0 0 1 0 0 0 0 0 0 1 1 1 0 0 1 3 0 0 0 0
Vac/Vac Membrane 0 4 1 12 26 40 71 99 75 106 76 94 2 3 6 28 5 13 4 8 2 1 1 0
Unique Cell Count 221 223 203 244 348 424 435 486 303 388 395 396 198 234 266 349 362 341 246 291 203 54 123 156
Labelled Cell Count 226 235 210 251 355 444 456 522 332 419 438 429 199 242 267 386 386 366 246 291 203 54 123 156


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 16.7 8.7 8.7 9.7 9.2 10.9 13.1 13.5 16.2 15.2 17.2 12.9 30.8 50.2 9.4 8.9 8.6 – 11.3 10.8
Std Deviation (1e-4) 15.1 30.6 3.0 6.5 6.0 5.4 5.9 8.7 7.6 10.3 11.0 11.6 6.6 19.3 37.2 4.1 2.5 3.0 – 5.2 3.9
Intensity Change (Log2) – – – 0.01 0.16 0.09 0.33 0.6 0.64 0.91 0.81 0.99 0.58 1.83 2.53 0.12 0.04 -0.01 – 0.38 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.1 0 0.6 0.2 0 0 0 0.1 0.7 0.5
Cytoplasm -2.3 -2.6 -3.8 -6.7 -7.7 -8.4 -8.8 -9.7 -9.0 0 -0.5 -0.9 -2.3 -1.0 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.3 3.3 4.5 3.2 6.3 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 3.7 4.2 5.9 6.8 7.5 8.0 6.5 7.4 0 0 0 3.8 0 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4233 11.0467 11.535 9.4841 8.6019 9.7297 11.2745 12.1659 13.8368 11.6122 10.8236 12.7552 6.753 9.1803 8.0484 7.0045 5.1931 7.1979
Actin 0.0054 0.0034 0.0071 0.0019 0.0433 0.0017 0.0053 0.0045 0.013 0.0038 0.039 0.0081 0.0239 0.0016 0.0146 0.006 0.0326 0.0073
Bud 0.0024 0.0144 0.0115 0.002 0.0089 0.0014 0.002 0.0287 0.0033 0.0024 0.0054 0.0015 0.0019 0.0085 0.0087 0.0047 0.0044 0.0077
Bud Neck 0.0074 0.0008 0.0009 0.0015 0.0021 0.0017 0.0004 0.0006 0.0028 0.0007 0.0015 0.0022 0.0027 0.0002 0.0016 0.0027 0.0013 0.0015
Bud Periphery 0.0025 0.0061 0.0118 0.0008 0.0056 0.0008 0.001 0.0179 0.0016 0.0016 0.0044 0.0014 0.0017 0.0021 0.0044 0.0021 0.0033 0.0079
Bud Site 0.0027 0.0219 0.003 0.0005 0.0046 0.0002 0.0019 0.0248 0.002 0.0016 0.0025 0.0008 0.0083 0.0025 0.0067 0.0043 0.0012 0.0007
Cell Periphery 0.0002 0.001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0007 0.0003 0.0001 0.0003 0.0002 0.0003 0.0002 0.0002 0.0003 0.0002 0.0001
Cytoplasm 0.8374 0.9059 0.9011 0.9463 0.7333 0.9445 0.907 0.8895 0.9052 0.8583 0.7673 0.924 0.7386 0.9421 0.822 0.8134 0.7383 0.9025
Cytoplasmic Foci 0.0334 0.0069 0.0045 0.0052 0.0162 0.0101 0.0099 0.0044 0.0082 0.0428 0.0324 0.0114 0.0494 0.0045 0.0291 0.0175 0.0329 0.016
Eisosomes 0 0 0 0 0.0003 0 0 0 0.0006 0 0.0001 0 0.0003 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0051 0.0017 0.003 0.0061 0.0085 0.003 0.0048 0.0016 0.0016 0.0044 0.0164 0.0042 0.0068 0.0012 0.0024 0.0046 0.0106 0.0019
Endosome 0.0246 0.002 0.0105 0.0048 0.0453 0.0036 0.0143 0.0006 0.0056 0.0065 0.0418 0.0063 0.0454 0.0066 0.0185 0.022 0.0144 0.0084
Golgi 0.0031 0.0005 0.002 0.0002 0.02 0.0002 0.0038 0.0001 0.0036 0.0009 0.0143 0.0036 0.0071 0.0001 0.0085 0.0059 0.0127 0.0028
Lipid Particles 0.0043 0.0004 0.0008 0.0001 0.0085 0.0001 0.0023 0.0001 0.0035 0.0082 0.0258 0.0008 0.013 0.0001 0.0073 0.0292 0.0247 0.004
Mitochondria 0.005 0.0004 0.0037 0.0003 0.0109 0.0003 0.0031 0.0005 0.0104 0.0003 0.0022 0.0009 0.0057 0.0003 0.012 0.0158 0.0032 0.0017
None 0.0261 0.0125 0.0176 0.0056 0.0054 0.0125 0.0197 0.0106 0.0171 0.0463 0.0091 0.0154 0.0456 0.0226 0.029 0.0395 0.0788 0.0119
Nuclear Periphery 0.0061 0.0025 0.0032 0.0044 0.0157 0.003 0.0075 0.0021 0.0031 0.0041 0.0059 0.0033 0.0094 0.0011 0.004 0.0037 0.0044 0.003
Nucleolus 0.0005 0.0003 0.0002 0.0001 0.0075 0 0.0002 0.0002 0.001 0.0001 0.0001 0.0001 0.0011 0.0001 0.0006 0.0005 0.0005 0.0001
Nucleus 0.0125 0.014 0.0103 0.0137 0.025 0.012 0.0084 0.0082 0.0104 0.0094 0.0123 0.0099 0.0094 0.0047 0.0083 0.0096 0.0151 0.0089
Peroxisomes 0.0097 0.0005 0.0019 0.0002 0.0041 0.0004 0.0035 0.0001 0.002 0.0014 0.0111 0.0005 0.0125 0.0001 0.0088 0.0101 0.0094 0.009
Punctate Nuclear 0.0047 0.0018 0.0015 0.0026 0.028 0.0015 0.0021 0.0022 0.0022 0.0043 0.0052 0.0039 0.0091 0.0007 0.0102 0.0033 0.0094 0.0031
Vacuole 0.0055 0.0028 0.0039 0.0031 0.004 0.0026 0.0021 0.0022 0.0014 0.0025 0.0022 0.0011 0.0057 0.0007 0.0022 0.0035 0.0019 0.001
Vacuole Periphery 0.0011 0.0002 0.0011 0.0005 0.0025 0.0005 0.0007 0.0003 0.001 0.0004 0.0007 0.0004 0.0022 0.0001 0.0007 0.0011 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4222 8.6524 8.9887 23.1886 17.9872 25.7963 18.955 24.9962 40.2736 26.2437
Translational Efficiency 0.9365 0.4859 0.7797 1.0374 1.0436 1.8165 0.8259 0.9464 1.3071 1.1665

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (48%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pgm2

Pgm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pgm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available