Standard name
Human Ortholog
Description Subunit of telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair; colocalizes with quiescent cell telomere hyperclusters
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.08 0.09 0.07 0.07 – 0.09 0 0 0.05 0.07 0.2 0.19 0.13 0.14 0.12 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.05 0 0.08 0 0.11 0.21 – 0.34 0.26 0.55 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.76 0.85 0.98 0.8 0.81 0.78 0.78 – 0.74 0.76 0.69 0.76 0.89 0.79 0.76 0.62 0.64 0.64 0.74 0.78 0.66 0.74 0.7 0.66
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.15 0.05 0.11 0.14 0.15 0.09 – 0.07 0.06 0.06 0.06 0 0.06 0.05 0.25 0.2 0.25 0.1 0.09 0.11 0.08 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 – 1 0 6 0 0 0 1 0 0 0 0 3 1 1 3 4
Bud Neck 0 0 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 3 – 3 3 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 11 1 11 18 17 16 – 18 10 11 11 2 39 43 29 33 23 0 7 2 5 9 7
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 0 0 0 1 0 1 0 3 13 12 0 0 0 0 2 0
Endosome 1 0 0 2 1 1 1 – 1 1 2 1 0 0 3 4 6 3 2 4 1 0 4 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 2 1 0 0 0 0 1 1
Mitochondria 18 10 0 10 0 28 51 – 69 71 145 62 1 0 0 6 7 3 0 4 1 2 3 9
Nucleus 57 180 63 107 155 196 193 – 150 205 182 177 25 156 170 143 147 126 78 186 57 79 183 197
Nuclear Periphery 2 0 0 1 0 1 2 – 1 6 6 1 0 0 0 1 4 0 1 2 3 0 0 1
Nucleolus 2 0 0 0 0 2 1 – 0 1 1 0 0 0 2 7 6 3 5 3 3 2 3 5
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 0 1 – 0 1 1 3 0 1 1 1 1 0 3 4 1 1 6 8
Vac/Vac Membrane 10 31 3 15 27 38 21 – 14 17 15 13 0 12 12 57 46 49 10 21 9 9 35 56
Unique Cell Count 75 212 64 133 191 250 246 203 269 265 232 28 198 224 230 228 196 106 239 86 108 261 301
Labelled Cell Count 93 232 67 147 204 283 291 258 315 374 273 28 210 233 254 266 220 106 239 86 108 261 301


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.5 5.7 6.1 6.6 5.3 5.3 – 4.7 4.7 4.3 4.6 6.3 6.2 6.4 10.1 10.7 9.7 5.8 6.1 6.3
Std Deviation (1e-4) 0.6 0.8 0.8 1.7 1.4 1.2 1.2 – 1.6 0.9 1.2 0.8 1.1 1.2 1.4 2.1 2.7 2.5 1.0 1.4 1.1
Intensity Change (Log2) – – – 0.1 0.21 -0.09 -0.11 – -0.28 -0.26 -0.39 -0.32 0.15 0.13 0.18 0.83 0.91 0.77 0.02 0.1 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 3.5 3.5 2.6 2.8 2.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.8 4.0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -3.4 -3.4 -3.8 -3.8 – -4.3 -4.0 -4.9 -4.0 -2.0 -3.7 -4.0 -5.6 -5.3 -5.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 2.0 2.2 0 – 0 0 0 0 0 0 0 3.5 2.9 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1676 0.1615 -0.0181 -0.1843 -0.3626 0.179 0.5984 1.0581 0.9763 1.0771 1.068 0.9911 0.171 0.29 -0.0468 0.3546 -0.0383 0.0467
Actin 0.0146 0.0005 0.0044 0.0047 0.0029 0.004 0.0079 0.011 0.0034 0.0025 0.0043 0.0002 0.0235 0.0004 0.012 0.0391 0.0307 0.0006
Bud 0.0003 0.0001 0.0041 0.0002 0.0015 0.0002 0.0003 0.0007 0.0003 0.0002 0.0015 0.0001 0.0003 0.0001 0.0008 0.0006 0.0009 0.0001
Bud Neck 0.0051 0.0005 0.0004 0.001 0.0081 0.0027 0.0015 0.0007 0.0004 0.0151 0.0094 0.0024 0.0032 0.0004 0.0038 0.0053 0.0026 0.0019
Bud Periphery 0.0004 0.0001 0.0014 0.0005 0.003 0.0002 0.0006 0.0013 0.0003 0.0003 0.0084 0.0001 0.0004 0.0001 0.0014 0.0008 0.0014 0.0001
Bud Site 0.004 0.001 0.002 0.0005 0.0162 0.0002 0.0054 0.0051 0.0012 0.0013 0.0044 0.0003 0.0073 0.0028 0.0061 0.0297 0.0035 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0008 0 0.0002 0.0002 0.0001 0.0002 0.0015 0 0.0002 0.0001 0.0002 0.0003 0.0001 0
Cytoplasm 0.0702 0.0733 0.0588 0.0442 0.1092 0.0188 0.0525 0.0948 0.0505 0.0312 0.049 0.0366 0.0493 0.0541 0.043 0.0288 0.1121 0.0351
Cytoplasmic Foci 0.0549 0.0049 0.0061 0.0144 0.0143 0.0052 0.0131 0.0034 0.0066 0.024 0.0068 0.0032 0.0106 0.0008 0.0054 0.0162 0.0297 0.0005
Eisosomes 0.0004 0 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0071 0.0107 0.0042 0.0019 0.0046 0.0013 0.0064 0.0048 0.0031 0.0011 0.003 0.0011 0.0089 0.0044 0.0031 0.0014 0.0059 0.002
Endosome 0.0456 0.0062 0.013 0.013 0.024 0.0245 0.0208 0.0043 0.0125 0.0298 0.0344 0.0061 0.0222 0.0021 0.0049 0.0074 0.0299 0.0015
Golgi 0.0127 0.0002 0.0018 0.0164 0.0057 0.0132 0.0027 0.0003 0.0022 0.022 0.0071 0.0006 0.0029 0.0001 0.0035 0.0058 0.0084 0
Lipid Particles 0.0227 0.0026 0.0015 0.0286 0.0055 0.0084 0.0095 0.0005 0.0027 0.0491 0.0161 0.0016 0.0052 0.0002 0.0106 0.0207 0.0303 0.0001
Mitochondria 0.0047 0.0006 0.0186 0.04 0.0235 0.0032 0.0031 0.0014 0.008 0.0067 0.0418 0.0008 0.0026 0.0008 0.0071 0.0106 0.0033 0.001
None 0.0782 0.0683 0.0572 0.0481 0.0097 0.0132 0.0566 0.0696 0.0533 0.0679 0.0375 0.0414 0.0571 0.0359 0.0172 0.0035 0.0103 0.0098
Nuclear Periphery 0.0531 0.111 0.1036 0.0426 0.0352 0.0394 0.0747 0.0686 0.0829 0.0325 0.0763 0.0276 0.0637 0.0778 0.0889 0.0538 0.049 0.0382
Nucleolus 0.0042 0.0033 0.003 0.0028 0.0131 0.005 0.0052 0.0037 0.0036 0.0083 0.0227 0.0104 0.0036 0.0062 0.0033 0.0134 0.0065 0.0055
Nucleus 0.5693 0.666 0.6657 0.7095 0.676 0.8024 0.6944 0.7024 0.7371 0.6689 0.6385 0.8404 0.7076 0.792 0.7395 0.7266 0.5985 0.854
Peroxisomes 0.0248 0.0002 0.0093 0.0071 0.0051 0.0124 0.0041 0.0003 0.0038 0.0182 0.0035 0.0007 0.0034 0.0001 0.006 0.0224 0.0341 0.0001
Punctate Nuclear 0.0191 0.0464 0.0403 0.0226 0.0338 0.0421 0.0359 0.0237 0.0253 0.0151 0.0173 0.0244 0.0225 0.0195 0.0409 0.0106 0.0379 0.0479
Vacuole 0.0066 0.0029 0.0026 0.001 0.0064 0.0024 0.0028 0.0019 0.0014 0.0047 0.0123 0.0014 0.003 0.0013 0.0011 0.0018 0.0035 0.001
Vacuole Periphery 0.0018 0.0012 0.0014 0.0006 0.0012 0.0012 0.002 0.0011 0.0012 0.0009 0.0042 0.0006 0.0023 0.0008 0.0011 0.001 0.0013 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2543 5.7408 3.8727 5.8744 5.2986 4.0844 5.6726 7.4792 5.1125 6.3008
Translational Efficiency 0.6207 0.5727 1.073 0.8012 0.7456 0.9162 0.8546 0.5255 0.8946 0.8345

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair; colocalizes with quiescent cell telomere hyperclusters
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yku80

Yku80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yku80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available