Standard name
Human Ortholog
Description One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.7 0.51 0.67 0.67 0.62 0.49 0.46 0.38 0.36 0.4 0.48 0.37 0.64 0.73 0.7 0.59 0.56 0.62 0.81 0.59 0.72 0.68 0.68 0.72
Bud 0.19 0.18 0.23 0.21 0.27 0.28 0.18 0.2 0.17 0.17 0.22 0.18 0.2 0.2 0.24 0.19 0.17 0.27 0.09 0.13 0 0.2 0.17 0.15
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0
Bud Site 0 0.06 0.1 0.13 0.14 0.08 0.06 0.05 0 0.06 0.07 0.05 0.06 0 0 0.06 0 0 – – – – – –
Cell Periphery 0.1 0 0 0.11 0.06 0.06 0.06 0 0.05 0.06 0.05 0.08 0.12 0.11 0.16 0.1 0.12 0.05 0 0 0 0 0 0
Cytoplasm 0.1 0.16 0.06 0 0.09 0.08 0.06 0.06 0.09 0.05 0.06 0.08 0.08 0.09 0.08 0.18 0.11 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.08 0 0 0.05 0.07 0.05 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0
Golgi 0.22 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.25 0.33 0.25 0 0 0 0 0 0
Mitochondria 0.07 0.45 0.53 0.75 0.31 0.55 0.64 0.63 0.63 0.67 0.71 0.65 0.3 0.28 0.35 0.08 0.09 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0.1 0.27 0.21 0.29 0.16 0.15 0.13 0.16 0.23 0.15 0.14 0.3 0.27 0.25 0 0 0 0 0 0.09 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 201 25 78 80 86 79 93 80 47 71 60 89 87 122 143 185 100 151 299 64 99 56 125 126
Bud 56 9 27 25 38 44 36 41 23 30 28 43 28 34 49 61 31 66 32 13 4 16 31 27
Bud Neck 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 8 7 3 3 9 6
Bud Site 12 3 12 16 19 13 12 10 5 11 9 12 8 4 2 18 5 10 – – – – – –
Cell Periphery 29 2 4 13 8 10 12 6 7 10 6 18 17 18 32 32 21 12 2 0 1 0 1 0
Cytoplasm 29 8 7 2 12 13 13 13 12 8 7 19 11 15 16 55 20 34 5 2 2 1 3 0
Endoplasmic Reticulum 5 4 4 4 7 12 10 13 5 4 3 5 3 9 6 6 1 5 1 0 1 0 1 0
Endosome 6 3 1 1 6 2 4 5 1 1 3 3 2 5 3 4 0 2 5 9 4 0 2 3
Golgi 65 0 2 2 3 3 8 4 2 4 3 2 5 9 9 79 59 60 1 2 0 0 2 2
Mitochondria 21 22 61 90 43 88 129 132 83 118 89 155 41 46 71 25 16 25 0 2 3 0 0 1
Nucleus 0 0 0 0 0 0 1 2 2 0 0 2 0 1 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 2 0 2 3 0 1 0 4 1 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 2 2 0 3 4 3 1 2 1 3 0 4 2 1 1 4 4 2 0 0 0 0 0 0
Peroxisomes 24 5 31 25 40 25 30 28 21 40 19 33 41 44 51 13 6 8 10 2 12 0 2 4
SpindlePole 13 0 1 1 0 1 3 2 2 1 1 4 2 1 2 9 8 6 1 0 1 0 0 0
Vac/Vac Membrane 5 3 2 1 2 5 4 4 3 2 2 2 0 0 1 12 11 1 0 0 1 0 0 1
Unique Cell Count 289 49 116 120 139 160 202 208 132 177 126 238 137 166 203 313 179 242 372 109 138 83 184 176
Labelled Cell Count 469 86 230 264 271 298 358 345 214 304 230 395 248 309 388 503 284 382 372 109 138 83 184 176


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 9.5 8.1 8.7 8.7 8.5 7.8 7.3 7.4 7.4 7.2 7.1 11.8 12.1 10.7 16.3 18.2 18.1 – 12.5 13.4
Std Deviation (1e-4) 3.4 2.5 2.4 2.8 1.9 2.1 1.7 1.5 1.6 1.5 1.5 1.7 2.6 2.7 2.5 5.1 6.0 5.9 – 2.4 2.5
Intensity Change (Log2) – – – 0.1 0.1 0.07 -0.05 -0.15 -0.13 -0.14 -0.17 -0.2 0.54 0.57 0.39 1.01 1.16 1.16 – 0.62 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -0.9 -3.0 -3.7 -5.0 -5.0 -4.5 -3.1 -5.3 -0.6 1.1 0.6 -1.5 -2.0 -0.9
Bud -0.5 0.7 0.8 -1.2 -0.8 -1.1 -1.3 -0.2 -1.2 -0.5 -0.6 0.2 -0.9 -1.3 0.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.7 0.8 -0.6 -1.4 -1.9 -2.0 -1.3 -0.9 -1.9 -1.3 -2.8 -3.9 -1.7 -2.7 -2.3
Cell Periphery 2.2 0.9 1.0 1.0 0 0.7 0.9 0 1.5 2.6 2.3 3.3 2.2 2.5 0.6
Cytoplasm 0 0.8 0.7 0.1 0.1 0.9 -0.6 -0.2 0.7 0.6 0.9 0.6 3.0 1.5 2.2
Endoplasmic Reticulum 0 0.6 1.4 0.6 1.1 0 0 0 0 0 0.8 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 5.5 6.5 5.4
Mitochondria 3.6 -3.5 0.4 2.0 1.9 1.6 2.4 2.9 2.3 -3.7 -4.2 -3.1 -10.2 -8.3 -8.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.1 0.4 -2.3 -2.6 -3.0 -2.1 -0.8 -2.2 -3.0 0.6 0 -0.3 -6.8 -5.9 -6.7
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8628 11.1831 10.8889 10.4473 9.5316 11.8988 10.2397 11.6589 10.8467 10.2379 10.6233 11.1504 10.4948 10.5893 10.0452 10.1695 10.9663 10.5942
Actin 0.9686 0.8378 0.9116 0.9543 0.938 0.9544 0.7949 0.731 0.9081 0.7978 0.9336 0.8777 0.8024 0.8555 0.8767 0.9227 0.9944 0.9466
Bud 0.0004 0.0018 0.0158 0.001 0.0006 0.0002 0.0095 0.006 0.0052 0.0204 0.0007 0.0016 0.0007 0.0096 0.0138 0.0037 0.0009 0.0003
Bud Neck 0.0032 0.0074 0.0148 0.02 0.0032 0.0277 0.0046 0.0052 0.0077 0.0174 0.0199 0.0691 0.0215 0.0042 0.0035 0.0014 0.0003 0.0325
Bud Periphery 0.0004 0.0019 0.0155 0.0021 0.001 0.0002 0.0115 0.0055 0.0071 0.0689 0.0012 0.0038 0.0007 0.0091 0.0207 0.0069 0.0014 0.0004
Bud Site 0.0163 0.1089 0.0368 0.0109 0.0013 0.0007 0.031 0.2329 0.0287 0.0044 0.0013 0.0008 0.0366 0.1126 0.0291 0.0051 0.0017 0.0017
Cell Periphery 0.0002 0.0013 0.0005 0.0009 0.0009 0.0001 0.0008 0.0006 0.0008 0.0012 0.0002 0.0002 0.0003 0.0005 0.0003 0.0002 0 0.0001
Cytoplasm 0.0002 0.0125 0.0018 0.001 0.0256 0.0001 0.01 0.0018 0.0046 0.0084 0.0004 0.0005 0.0043 0.0003 0.0006 0.0004 0 0.0005
Cytoplasmic Foci 0.0017 0.01 0.0006 0.0018 0.0017 0.002 0.0181 0.0083 0.0116 0.0134 0.0016 0.0022 0.0227 0.0012 0.0227 0.0049 0.0001 0.0058
Eisosomes 0.002 0.0009 0.0006 0.0009 0.0008 0.0037 0.0019 0.001 0.0005 0.0005 0.0005 0.0012 0.0054 0.0013 0.0006 0.0007 0.0003 0.0011
Endoplasmic Reticulum 0.0001 0.0001 0 0.0002 0.0129 0.0001 0.0187 0.0001 0.0005 0.0028 0.0001 0.0005 0.0011 0 0.0003 0.0001 0 0.0003
Endosome 0.0003 0.0014 0.0001 0.0004 0.0043 0.0008 0.0297 0.0004 0.0075 0.0228 0.0052 0.016 0.0071 0 0.0035 0.004 0 0.002
Golgi 0.0022 0.0059 0.0008 0.0041 0.0017 0.0057 0.0121 0.0006 0.0038 0.0172 0.0113 0.0109 0.0071 0.0007 0.004 0.0121 0.0002 0.0019
Lipid Particles 0.0004 0.002 0.0001 0.0002 0.0013 0.0011 0.0075 0.0004 0.0007 0.0035 0.0001 0.0008 0.0125 0.0001 0.0044 0.0012 0 0.0008
Mitochondria 0.0006 0.0022 0.0002 0.0002 0.0002 0.0004 0.011 0.0002 0.0046 0.002 0.0227 0.0098 0.0051 0.0001 0.0043 0.034 0.0001 0.0012
None 0 0.0001 0 0 0.0001 0.0001 0.0018 0.0027 0.0002 0.0015 0 0.0001 0.013 0.0001 0.0005 0 0 0.0002
Nuclear Periphery 0 0 0 0.0001 0.0006 0 0.02 0 0.0005 0.0031 0 0.0004 0.0142 0 0.0008 0 0 0.0002
Nucleolus 0 0.0001 0 0 0 0 0.0003 0 0.0002 0.0002 0 0 0.0014 0 0.0004 0 0 0
Nucleus 0 0.0001 0 0.0001 0.0003 0 0.002 0.0001 0.0008 0.0007 0 0.0001 0.0052 0 0.0005 0 0 0.0001
Peroxisomes 0.0034 0.0051 0.0008 0.0018 0.0028 0.0024 0.0073 0.0019 0.0031 0.001 0.0007 0.0025 0.0317 0.0045 0.0112 0.0021 0.0004 0.0016
Punctate Nuclear 0 0.0001 0 0.0001 0.0001 0.0001 0.0014 0.0011 0.001 0.0007 0 0.0001 0.0055 0.0001 0.0015 0.0001 0 0.0025
Vacuole 0 0.0004 0.0001 0.0001 0.0025 0 0.0032 0.0001 0.0024 0.0084 0.0001 0.0012 0.0007 0 0.0004 0.0002 0 0.0001
Vacuole Periphery 0 0 0 0 0.0002 0 0.0027 0 0.0004 0.0037 0.0002 0.0007 0.0008 0 0.0002 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.5634 35.0352 43.996 55.0367 40.4558 41.2794 52.3868 58.6687 58.4171 56.5468
Translational Efficiency 0.6713 0.7051 0.6011 0.5741 0.5754 0.6955 0.5872 0.4862 0.5201 0.5387

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
Localization
Cell Percentages actin (95%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Myo5

Myo5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Myo5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available