Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.11 0 0.07 0.05 0.09 0 0 0.1 0 0 0 0.07 0.13 0.17 0.1 0.2 0.18 0.05 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.06 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Mitochondria 0.34 0 0.44 0.34 0.05 0.34 0.24 0.23 0.27 0.38 0.36 0.41 0.05 0 0 0.05 0.1 0.08 0 0 0 0 0 0
Nucleus 0.49 0.52 0.81 0.71 0.88 0.61 0.76 0.72 0.63 0.7 0.61 0.68 0.85 0.83 0.71 0.63 0.6 0.57 0.52 0.63 0.7 0.55 0.66 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.1 0.15 0.08 0.25 0.23 0.3 0.24 0.35 0.38 0.34 0.14 0.13 0.16 0.22 0.05 0 0.06 0.08 0.09 0.17 0.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.29 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.12 0.15 0.14 0.16 0.11 0.07 0.05 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 2 0 0 1 1 1 0 2 2 0 0 0 1 0 0 1 4 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 1 2 1 4 4 8 2 5 8 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 3 8 7 17 8 13 21 8 7 8 15 27 41 4 4 9 3 3 0 4 1 0
Endoplasmic Reticulum 1 0 0 1 0 2 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 0 4 0 0 3 1 1 1 1 0 0 0 1 0 4 2 2 3 3 1 2 1 2 9
Golgi 0 1 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 3 1 0 5 1 0
Mitochondria 12 2 32 41 8 64 61 85 54 85 78 111 12 3 1 2 2 4 0 1 3 1 2 3
Nucleus 17 32 59 87 136 116 192 267 127 156 134 183 188 170 173 26 12 28 37 59 57 44 125 155
Nuclear Periphery 0 0 1 2 3 2 4 5 5 3 4 2 2 0 2 0 0 1 0 0 0 0 0 0
Nucleolus 2 3 7 18 12 47 59 110 48 79 84 93 32 26 38 9 1 1 4 7 7 13 37 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 3 1 0 1 1 2 1 2 0 0 0 2 3 1 0 1 6
Vac/Vac Membrane 6 18 2 3 5 6 5 11 10 3 2 2 3 4 6 5 3 7 11 10 5 4 11 20
Unique Cell Count 35 62 73 122 154 191 253 369 201 224 219 270 222 205 244 41 20 49 72 95 82 80 189 260
Labelled Cell Count 41 67 106 164 176 259 336 507 270 340 320 413 257 232 268 50 25 56 72 95 82 80 189 260


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.7 5.2 5.7 6.7 5.1 5.3 5.4 7.1 5.2 5.2 5.0 6.9 7.1 7.0 9.0 8.9 8.4 8.8 7.5 7.5
Std Deviation (1e-4) 3.2 1.5 2.1 2.9 3.2 2.1 2.0 2.2 5.4 2.1 2.0 1.7 1.5 2.0 2.2 2.3 2.5 2.8 4.2 1.7 2.1
Intensity Change (Log2) 0.11 0.36 -0.05 0.03 0.05 0.44 -0.0 -0.01 -0.06 0.39 0.44 0.42 0.79 0.76 0.68 0.74 0.51 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.3 0 0 1.6 0 0 0 0 2.1 2.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.4 -7.1 -1.6 -3.3 -3.7 -2.7 -0.9 -1.3 -0.4 -8.0 -9.4 -10.7 -4.4 -2.8 -4.2
Nucleus -1.5 1.5 -3.1 -0.9 -1.5 -2.8 -1.9 -3.1 -2.2 0.8 0.4 -1.7 -2.0 -1.9 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 -0.5 2.7 2.6 3.6 2.6 4.2 4.6 4.1 1.1 0.7 1.3 1.8 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7867 0.5384 0.7401 1.2074 1.646 0.4679 1.3802 0.076 0.1151 -0.0288 1.6504 0.3173 3.1309 1.6383 1.7121 2.2343 3.987 1.7724
Actin 0.076 0.0002 0.0323 0.0193 0.017 0.0001 0.0945 0.0021 0.0306 0.0035 0.1054 0.0029 0.0782 0.0001 0.0104 0.0476 0.197 0.0008
Bud 0.0008 0.0001 0.0011 0.0012 0.0008 0.0003 0.001 0.0016 0.0013 0.0002 0.0046 0.0003 0.0009 0.0002 0.0004 0.0017 0.0013 0.0001
Bud Neck 0.0014 0.0004 0.0054 0.1068 0.0004 0.0073 0.0054 0.0007 0.0012 0.0005 0.003 0.0024 0.0129 0.0003 0.0127 0.0006 0.0212 0.0036
Bud Periphery 0.0013 0 0.0011 0.0015 0.0019 0.0005 0.0019 0.0003 0.0015 0.0004 0.0093 0.0009 0.0017 0.0001 0.0007 0.0024 0.0035 0.0003
Bud Site 0.0026 0.0008 0.0119 0.0224 0.0018 0.0006 0.0036 0.0039 0.0144 0.0009 0.0138 0.0005 0.0083 0.0022 0.006 0.0072 0.0028 0.0002
Cell Periphery 0.0003 0 0.0003 0.0009 0.0004 0.0001 0.0005 0.0001 0.0002 0.0002 0.0005 0.0001 0.0009 0.0001 0.0002 0.0001 0.0007 0
Cytoplasm 0.0245 0.0149 0.0066 0.0026 0.006 0.0038 0.0232 0.0084 0.0027 0.0094 0.0095 0.0018 0.0053 0.016 0.0038 0.0036 0.0033 0.005
Cytoplasmic Foci 0.0303 0.0059 0.0448 0.0971 0.064 0.0003 0.0412 0.0039 0.0124 0.0064 0.0275 0.0042 0.0673 0.0004 0.0029 0.0068 0.0169 0.0003
Eisosomes 0.0006 0 0.0001 0.0003 0.0004 0 0.0008 0 0.0001 0.0012 0.001 0.0001 0.001 0 0.0001 0.0003 0.0016 0.0001
Endoplasmic Reticulum 0.0129 0.0009 0.0172 0.0005 0.0021 0.0002 0.0048 0.0003 0.002 0.0007 0.0034 0.001 0.0029 0.0005 0.0003 0.0005 0.0134 0.0004
Endosome 0.0397 0.0011 0.01 0.0544 0.0763 0.0009 0.0543 0.0012 0.0161 0.038 0.0209 0.0069 0.0393 0.0004 0.01 0.0086 0.0346 0.0011
Golgi 0.0116 0.0005 0.0047 0.0547 0.0292 0.0001 0.0301 0.0016 0.0153 0.0372 0.0216 0.004 0.0308 0 0.0019 0.0114 0.0145 0.0006
Lipid Particles 0.0167 0.0013 0.0087 0.0335 0.0734 0 0.0502 0.0004 0.0043 0.0394 0.0514 0.0205 0.0878 0 0.0038 0.0254 0.0353 0.0026
Mitochondria 0.0341 0.0004 0.0013 0.0111 0.2876 0.0003 0.0453 0.0007 0.0094 0.0471 0.158 0.0044 0.024 0.0004 0.0019 0.0392 0.0069 0.0057
None 0.0532 0.0389 0.0026 0.0005 0.0078 0.0003 0.0092 0.0095 0.0009 0.007 0.0027 0.0006 0.0045 0.0278 0.0014 0.054 0.0143 0.0147
Nuclear Periphery 0.0355 0.0082 0.0056 0.0011 0.0114 0.0035 0.0216 0.0106 0.011 0.0147 0.1305 0.0191 0.0086 0.0093 0.0026 0.0026 0.0854 0.0054
Nucleolus 0.0098 0.0221 0.0152 0.0038 0.0028 0.0633 0.0133 0.0198 0.0099 0.0146 0.0086 0.0515 0.0104 0.0196 0.0182 0.007 0.0296 0.0594
Nucleus 0.5859 0.8528 0.798 0.5504 0.2523 0.9082 0.5401 0.891 0.8311 0.7414 0.3193 0.8227 0.5325 0.8941 0.8964 0.7633 0.374 0.8724
Peroxisomes 0.0108 0.0051 0.0203 0.0302 0.0173 0 0.0237 0.001 0.0103 0.0071 0.0718 0.0028 0.0436 0 0.0015 0.0115 0.0583 0.0002
Punctate Nuclear 0.047 0.0454 0.0086 0.0052 0.1269 0.0082 0.0221 0.0423 0.0226 0.0258 0.0314 0.0499 0.0298 0.0277 0.0187 0.0052 0.0759 0.0264
Vacuole 0.0023 0.0009 0.0038 0.0017 0.0088 0.0018 0.0083 0.0004 0.0017 0.0013 0.0027 0.0016 0.0055 0.0006 0.0043 0.0005 0.0038 0.0005
Vacuole Periphery 0.0026 0.0002 0.0003 0.0007 0.0115 0.0002 0.0048 0.0001 0.001 0.003 0.0032 0.0019 0.0039 0.0001 0.0017 0.0005 0.0057 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5387 12.4542 12.9382 15.0199 25.2886 8.3545 16.3702 11.8987 13.753 17.184
Translational Efficiency 2.3256 1.4362 1.5712 0.9972 0.8799 3.8757 1.7056 2.0449 1.7509 1.683

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Med11

Med11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available