Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 4 | 4 | 8 | 2 | 5 | 8 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 3 | 8 | 7 | 17 | 8 | 13 | 21 | 8 | 7 | 8 | 15 | 27 | 41 | 4 | 4 | 9 | 3 | 3 | 0 | 4 | 1 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 2 | 3 | 3 | 1 | 2 | 1 | 2 | 9 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 5 | 1 | 0 |
Mitochondria | 12 | 2 | 32 | 41 | 8 | 64 | 61 | 85 | 54 | 85 | 78 | 111 | 12 | 3 | 1 | 2 | 2 | 4 | 0 | 1 | 3 | 1 | 2 | 3 |
Nucleus | 17 | 32 | 59 | 87 | 136 | 116 | 192 | 267 | 127 | 156 | 134 | 183 | 188 | 170 | 173 | 26 | 12 | 28 | 37 | 59 | 57 | 44 | 125 | 155 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 5 | 5 | 3 | 4 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 3 | 7 | 18 | 12 | 47 | 59 | 110 | 48 | 79 | 84 | 93 | 32 | 26 | 38 | 9 | 1 | 1 | 4 | 7 | 7 | 13 | 37 | 52 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 6 |
Vac/Vac Membrane | 6 | 18 | 2 | 3 | 5 | 6 | 5 | 11 | 10 | 3 | 2 | 2 | 3 | 4 | 6 | 5 | 3 | 7 | 11 | 10 | 5 | 4 | 11 | 20 |
Unique Cell Count | 35 | 62 | 73 | 122 | 154 | 191 | 253 | 369 | 201 | 224 | 219 | 270 | 222 | 205 | 244 | 41 | 20 | 49 | 72 | 95 | 82 | 80 | 189 | 260 |
Labelled Cell Count | 41 | 67 | 106 | 164 | 176 | 259 | 336 | 507 | 270 | 340 | 320 | 413 | 257 | 232 | 268 | 50 | 25 | 56 | 72 | 95 | 82 | 80 | 189 | 260 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.7 | 5.2 | 5.7 | 6.7 | 5.1 | 5.3 | 5.4 | 7.1 | 5.2 | 5.2 | 5.0 | 6.9 | 7.1 | 7.0 | 9.0 | 8.9 | 8.4 | 8.8 | 7.5 | 7.5 |
Std Deviation (1e-4) | 3.2 | 1.5 | 2.1 | 2.9 | 3.2 | 2.1 | 2.0 | 2.2 | 5.4 | 2.1 | 2.0 | 1.7 | 1.5 | 2.0 | 2.2 | 2.3 | 2.5 | 2.8 | 4.2 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.36 | -0.05 | 0.03 | 0.05 | 0.44 | -0.0 | -0.01 | -0.06 | 0.39 | 0.44 | 0.42 | 0.79 | 0.76 | 0.68 | 0.74 | 0.51 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.3 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 2.1 | 2.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.4 | -7.1 | -1.6 | -3.3 | -3.7 | -2.7 | -0.9 | -1.3 | -0.4 | -8.0 | -9.4 | -10.7 | -4.4 | -2.8 | -4.2 |
Nucleus | -1.5 | 1.5 | -3.1 | -0.9 | -1.5 | -2.8 | -1.9 | -3.1 | -2.2 | 0.8 | 0.4 | -1.7 | -2.0 | -1.9 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.0 | -0.5 | 2.7 | 2.6 | 3.6 | 2.6 | 4.2 | 4.6 | 4.1 | 1.1 | 0.7 | 1.3 | 1.8 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7867 | 0.5384 | 0.7401 | 1.2074 | 1.646 | 0.4679 | 1.3802 | 0.076 | 0.1151 | -0.0288 | 1.6504 | 0.3173 | 3.1309 | 1.6383 | 1.7121 | 2.2343 | 3.987 | 1.7724 |
Actin | 0.076 | 0.0002 | 0.0323 | 0.0193 | 0.017 | 0.0001 | 0.0945 | 0.0021 | 0.0306 | 0.0035 | 0.1054 | 0.0029 | 0.0782 | 0.0001 | 0.0104 | 0.0476 | 0.197 | 0.0008 |
Bud | 0.0008 | 0.0001 | 0.0011 | 0.0012 | 0.0008 | 0.0003 | 0.001 | 0.0016 | 0.0013 | 0.0002 | 0.0046 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0017 | 0.0013 | 0.0001 |
Bud Neck | 0.0014 | 0.0004 | 0.0054 | 0.1068 | 0.0004 | 0.0073 | 0.0054 | 0.0007 | 0.0012 | 0.0005 | 0.003 | 0.0024 | 0.0129 | 0.0003 | 0.0127 | 0.0006 | 0.0212 | 0.0036 |
Bud Periphery | 0.0013 | 0 | 0.0011 | 0.0015 | 0.0019 | 0.0005 | 0.0019 | 0.0003 | 0.0015 | 0.0004 | 0.0093 | 0.0009 | 0.0017 | 0.0001 | 0.0007 | 0.0024 | 0.0035 | 0.0003 |
Bud Site | 0.0026 | 0.0008 | 0.0119 | 0.0224 | 0.0018 | 0.0006 | 0.0036 | 0.0039 | 0.0144 | 0.0009 | 0.0138 | 0.0005 | 0.0083 | 0.0022 | 0.006 | 0.0072 | 0.0028 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0003 | 0.0009 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0 |
Cytoplasm | 0.0245 | 0.0149 | 0.0066 | 0.0026 | 0.006 | 0.0038 | 0.0232 | 0.0084 | 0.0027 | 0.0094 | 0.0095 | 0.0018 | 0.0053 | 0.016 | 0.0038 | 0.0036 | 0.0033 | 0.005 |
Cytoplasmic Foci | 0.0303 | 0.0059 | 0.0448 | 0.0971 | 0.064 | 0.0003 | 0.0412 | 0.0039 | 0.0124 | 0.0064 | 0.0275 | 0.0042 | 0.0673 | 0.0004 | 0.0029 | 0.0068 | 0.0169 | 0.0003 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0008 | 0 | 0.0001 | 0.0012 | 0.001 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0003 | 0.0016 | 0.0001 |
Endoplasmic Reticulum | 0.0129 | 0.0009 | 0.0172 | 0.0005 | 0.0021 | 0.0002 | 0.0048 | 0.0003 | 0.002 | 0.0007 | 0.0034 | 0.001 | 0.0029 | 0.0005 | 0.0003 | 0.0005 | 0.0134 | 0.0004 |
Endosome | 0.0397 | 0.0011 | 0.01 | 0.0544 | 0.0763 | 0.0009 | 0.0543 | 0.0012 | 0.0161 | 0.038 | 0.0209 | 0.0069 | 0.0393 | 0.0004 | 0.01 | 0.0086 | 0.0346 | 0.0011 |
Golgi | 0.0116 | 0.0005 | 0.0047 | 0.0547 | 0.0292 | 0.0001 | 0.0301 | 0.0016 | 0.0153 | 0.0372 | 0.0216 | 0.004 | 0.0308 | 0 | 0.0019 | 0.0114 | 0.0145 | 0.0006 |
Lipid Particles | 0.0167 | 0.0013 | 0.0087 | 0.0335 | 0.0734 | 0 | 0.0502 | 0.0004 | 0.0043 | 0.0394 | 0.0514 | 0.0205 | 0.0878 | 0 | 0.0038 | 0.0254 | 0.0353 | 0.0026 |
Mitochondria | 0.0341 | 0.0004 | 0.0013 | 0.0111 | 0.2876 | 0.0003 | 0.0453 | 0.0007 | 0.0094 | 0.0471 | 0.158 | 0.0044 | 0.024 | 0.0004 | 0.0019 | 0.0392 | 0.0069 | 0.0057 |
None | 0.0532 | 0.0389 | 0.0026 | 0.0005 | 0.0078 | 0.0003 | 0.0092 | 0.0095 | 0.0009 | 0.007 | 0.0027 | 0.0006 | 0.0045 | 0.0278 | 0.0014 | 0.054 | 0.0143 | 0.0147 |
Nuclear Periphery | 0.0355 | 0.0082 | 0.0056 | 0.0011 | 0.0114 | 0.0035 | 0.0216 | 0.0106 | 0.011 | 0.0147 | 0.1305 | 0.0191 | 0.0086 | 0.0093 | 0.0026 | 0.0026 | 0.0854 | 0.0054 |
Nucleolus | 0.0098 | 0.0221 | 0.0152 | 0.0038 | 0.0028 | 0.0633 | 0.0133 | 0.0198 | 0.0099 | 0.0146 | 0.0086 | 0.0515 | 0.0104 | 0.0196 | 0.0182 | 0.007 | 0.0296 | 0.0594 |
Nucleus | 0.5859 | 0.8528 | 0.798 | 0.5504 | 0.2523 | 0.9082 | 0.5401 | 0.891 | 0.8311 | 0.7414 | 0.3193 | 0.8227 | 0.5325 | 0.8941 | 0.8964 | 0.7633 | 0.374 | 0.8724 |
Peroxisomes | 0.0108 | 0.0051 | 0.0203 | 0.0302 | 0.0173 | 0 | 0.0237 | 0.001 | 0.0103 | 0.0071 | 0.0718 | 0.0028 | 0.0436 | 0 | 0.0015 | 0.0115 | 0.0583 | 0.0002 |
Punctate Nuclear | 0.047 | 0.0454 | 0.0086 | 0.0052 | 0.1269 | 0.0082 | 0.0221 | 0.0423 | 0.0226 | 0.0258 | 0.0314 | 0.0499 | 0.0298 | 0.0277 | 0.0187 | 0.0052 | 0.0759 | 0.0264 |
Vacuole | 0.0023 | 0.0009 | 0.0038 | 0.0017 | 0.0088 | 0.0018 | 0.0083 | 0.0004 | 0.0017 | 0.0013 | 0.0027 | 0.0016 | 0.0055 | 0.0006 | 0.0043 | 0.0005 | 0.0038 | 0.0005 |
Vacuole Periphery | 0.0026 | 0.0002 | 0.0003 | 0.0007 | 0.0115 | 0.0002 | 0.0048 | 0.0001 | 0.001 | 0.003 | 0.0032 | 0.0019 | 0.0039 | 0.0001 | 0.0017 | 0.0005 | 0.0057 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5387 | 12.4542 | 12.9382 | 15.0199 | 25.2886 | 8.3545 | 16.3702 | 11.8987 | 13.753 | 17.184 |
Translational Efficiency | 2.3256 | 1.4362 | 1.5712 | 0.9972 | 0.8799 | 3.8757 | 1.7056 | 2.0449 | 1.7509 | 1.683 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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