Standard name
Human Ortholog
Description Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0.05 0.08
Bud 0 0 0.06 0.05 0.06 0.05 0.05 0.05 0.07 0 0.06 0.05 0 0 0 – 0.06 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.21 0.35 0.45 0.3 0.28 0.13 0.14 0.15 0.1 0.07 0.07 0 0.28 0.34 0.48 – 0.21 – 0.05 0.09 0.06 0 0 0
Endoplasmic Reticulum 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.18 – 0.24 – 0.1 0.06 0.08 0.14 0.09 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.85 0.67 0.57 0.74 0.64 0.87 0.9 0.84 0.93 0.94 0.94 0.97 0.57 0.38 0.27 – 0.24 – 0.67 0.6 0.62 0.59 0.57 0.51
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.1 – 0.09 – 0 0 0 0.07 0.1 0
SpindlePole 0 0 0 0.05 0.06 0 0.06 0.09 0 0 0.06 0 0 0.05 0 – 0.15 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0.06 0 0 0 0 0 0 0 0.09 0.06 0.05 – 0.21 – 0 0.11 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 1 0 0 0 0 0 1 0 0 4 0 0 – 0 – 5 5 6 4 13 26
Bud 3 7 10 8 11 13 18 24 18 15 19 21 7 8 11 – 2 – 0 5 1 2 3 9
Bud Neck 0 0 2 1 1 0 0 1 2 0 0 0 0 0 0 – 0 – 0 4 4 1 3 10
Bud Site 1 0 1 1 0 4 5 8 7 5 4 9 1 0 1 – 0 – – – – – – –
Cell Periphery 1 2 3 0 2 0 2 1 0 3 1 5 1 6 4 – 1 – 1 0 0 0 0 2
Cytoplasm 54 63 72 45 52 35 53 68 26 28 22 18 49 97 148 – 7 – 12 19 12 6 8 12
Endoplasmic Reticulum 15 9 4 2 1 4 5 3 4 4 0 3 4 6 6 – 2 – 3 3 3 3 1 5
Endosome 0 2 0 2 8 2 1 1 0 0 0 0 15 40 56 – 8 – 27 13 16 24 22 41
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 – 1 – 1 2 2 1 5 4
Mitochondria 214 120 90 111 118 235 342 374 246 374 280 401 101 111 82 – 8 – 185 131 123 101 135 163
Nucleus 0 1 1 0 0 2 2 9 3 3 3 3 0 2 5 – 1 – 0 0 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 3 0 0 13 3 5 0 0 0 – 0 – 2 3 0 0 1 0
Nucleolus 0 0 0 0 0 0 2 1 1 1 1 1 0 1 1 – 0 – 1 0 0 0 1 0
Peroxisomes 1 0 0 4 6 1 4 4 0 0 1 2 21 35 31 – 3 – 11 2 5 12 22 12
SpindlePole 4 2 6 8 12 11 21 39 7 10 18 18 6 15 6 – 5 – 9 3 5 4 9 12
Vac/Vac Membrane 8 19 6 2 11 9 8 10 3 7 12 11 16 16 16 – 7 – 7 23 14 4 4 11
Unique Cell Count 252 178 159 151 185 270 379 443 265 399 298 414 177 289 308 34 277 221 200 173 237 323
Labelled Cell Count 305 228 196 185 223 316 466 543 317 464 364 497 226 337 367 45 277 221 200 173 237 323


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.8 3.4 3.4 3.7 3.1 2.7 3.0 3.0 2.7 3.0 2.8 4.6 4.6 4.6 4.4 5.6 – 4.3 4.4 5.1
Std Deviation (1e-4) 0.4 0.7 0.9 0.9 1.2 1.1 0.5 0.6 0.9 0.6 1.0 0.9 1.3 1.2 1.5 1.3 1.7 – 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.01 0.11 -0.17 -0.33 -0.18 -0.2 -0.33 -0.19 -0.32 0.43 0.41 0.41 0.34 0.69 – 0.33 0.36 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1394 2.7216 2.5157 2.3637 1.7696 2.4351 0.7172 1.2935 1.0344 1.0182 0.8242 0.986 1.6332 1.8611 1.8444 1.3072 1.5683 1.6113
Actin 0.0961 0.0664 0.0507 0.0078 0.0138 0.0369 0.0339 0.0543 0.0356 0.044 0.0262 0.0333 0.1705 0.0507 0.0362 0.0007 0.03 0.0537
Bud 0.0007 0.003 0.0021 0.0076 0.0004 0.0005 0.0008 0.0003 0.0009 0.0005 0.0005 0.0003 0.0026 0.0007 0.0035 0.0005 0.0007 0.0007
Bud Neck 0.0024 0.0028 0.0077 0.0008 0.0009 0.0233 0.0062 0.0042 0.0038 0.0052 0.0006 0.0013 0.0033 0.0006 0.0061 0.0002 0.0006 0.0015
Bud Periphery 0.0011 0.0039 0.0047 0.0301 0.0007 0.001 0.002 0.0005 0.0021 0.0011 0.0009 0.0005 0.0032 0.0008 0.0053 0.0005 0.0012 0.0024
Bud Site 0.0044 0.0568 0.0172 0.0014 0.001 0.001 0.0064 0.029 0.0143 0.0014 0.0005 0.0005 0.0171 0.0216 0.0217 0.0007 0.0017 0.0008
Cell Periphery 0.0004 0.0013 0.0032 0.0008 0.0002 0.0005 0.0098 0.0003 0.0026 0.0004 0.0001 0.0003 0.0004 0.0002 0.0006 0.0001 0.001 0.0003
Cytoplasm 0.0041 0.0158 0.0065 0.0017 0.0006 0.0015 0.0052 0.0035 0.006 0.0014 0.0035 0.0018 0.0142 0.0012 0.0035 0.0164 0.0067 0.0141
Cytoplasmic Foci 0.123 0.0723 0.0633 0.0449 0.0477 0.0559 0.103 0.0516 0.0584 0.0732 0.0783 0.0499 0.0878 0.0478 0.0583 0.021 0.0722 0.0611
Eisosomes 0.0009 0.0009 0.001 0.001 0.0003 0.0005 0.0024 0.0005 0.002 0.0013 0.0002 0.0007 0.0021 0.0004 0.001 0.0001 0.0009 0.001
Endoplasmic Reticulum 0.0036 0.0006 0.0023 0.0006 0.0003 0.0005 0.0044 0.0009 0.0009 0.0001 0.0057 0.0003 0.0013 0.0006 0.0005 0.001 0.0014 0.0005
Endosome 0.124 0.0549 0.0763 0.0748 0.0765 0.0662 0.0929 0.0493 0.0457 0.038 0.1007 0.0343 0.0389 0.0491 0.0743 0.1479 0.0423 0.0567
Golgi 0.0822 0.0718 0.0767 0.1371 0.1087 0.1032 0.07 0.0758 0.0497 0.0835 0.0788 0.069 0.094 0.0406 0.0991 0.0164 0.1503 0.1031
Lipid Particles 0.1071 0.0234 0.05 0.0669 0.0468 0.0847 0.1192 0.0413 0.0284 0.039 0.0411 0.0642 0.0268 0.0433 0.0468 0.0026 0.0164 0.0497
Mitochondria 0.1959 0.4118 0.4293 0.5002 0.593 0.4538 0.2596 0.3432 0.4473 0.4368 0.2829 0.454 0.1431 0.5426 0.3714 0.7133 0.4674 0.3599
None 0.0106 0.0237 0.0127 0.0228 0.0004 0.0005 0.0096 0.0236 0.0208 0.0242 0.0028 0.0131 0.0061 0.0004 0.0327 0.0027 0.0958 0.0019
Nuclear Periphery 0.0028 0.0002 0.0063 0.0004 0.0002 0.0003 0.0054 0.0014 0.0003 0.0001 0.0076 0.0003 0.0244 0.0003 0.0003 0.0008 0.0003 0.0005
Nucleolus 0.0006 0.0007 0.001 0.0008 0.0002 0.0004 0.0017 0.0005 0.001 0.0014 0.0002 0.0003 0.0009 0.0002 0.001 0.0003 0.001 0.0001
Nucleus 0.0008 0.0004 0.0013 0.0005 0.0002 0.0003 0.001 0.0002 0.0005 0.0005 0.0011 0.0002 0.0618 0.0001 0.0005 0.0006 0.0007 0.0003
Peroxisomes 0.2237 0.1836 0.1783 0.0888 0.0998 0.1566 0.2435 0.3148 0.2719 0.2451 0.3627 0.2717 0.2925 0.1905 0.2262 0.0092 0.1042 0.2762
Punctate Nuclear 0.0059 0.0004 0.0013 0.0006 0.0004 0.0005 0.0062 0.0005 0.0018 0.0013 0.002 0.0006 0.0067 0.0003 0.0059 0.0002 0.0005 0.0004
Vacuole 0.0063 0.0038 0.0048 0.0065 0.0029 0.0068 0.014 0.0017 0.0042 0.0008 0.0016 0.0016 0.0013 0.0059 0.0034 0.0556 0.003 0.0075
Vacuole Periphery 0.0035 0.0017 0.0031 0.0036 0.0051 0.0051 0.0027 0.0027 0.0016 0.0007 0.002 0.0018 0.001 0.0021 0.0018 0.0092 0.0017 0.0075

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8812 21.6138 20.8985 21.0308 16.149 18.3067 20.8604 23.0186 25.4309 17.1391
Translational Efficiency 1.0315 0.8181 0.8664 0.9515 0.9839 1.0686 0.9371 0.8411 0.7729 1.0279

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
Localization
Cell Percentages mitochondrion (62%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mgr3

Mgr3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mgr3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available