Standard name
Human Ortholog
Description V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0.06 0 0.09 0.11 0.1 0.12 0.1 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.05 0.19 0.09 0 0.07 0.08 0.1 0.14 0.08 0.15 0.26 0.24 0.37 0.29 0.27 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.06 0.08 0.09 0.12 0.09 0.13 0.14 0.16 0 0.05 0.05 0.15 0.18 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0.9 0.96 0.88 0.79 0.86 0.78 0.61 0.66 0.6 0.53 0.56 0.89 0.82 0.84 0 0 0 0.93 0.95 0.93 0.93 0.9 0.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.26 0.37 0.46 0 0 0 0 0 0
Mitochondria 0 0 0 0.19 0.06 0.13 0.12 0.23 0.14 0.29 0.23 0 0 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0.07 0.08 0.12 0 0.1 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.06 0 0 0 0.09 0.06 0.11 0 0.07 0 0 0 0.3 0.22 0.25 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 4 9 12 14 11 30 38 41 33 24 22 19 11 2 2 2 12 1 2 2 10 4
Bud 5 0 8 0 3 2 7 2 4 5 5 0 0 0 0 1 1 4 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 24 12 15 39 23 12 22 29 42 39 20 64 92 82 17 20 18 5 3 2 2 4 8
Cytoplasm 68 11 13 12 21 25 40 33 52 38 39 13 19 19 7 12 9 19 2 1 3 6 7
Endoplasmic Reticulum 817 251 239 166 217 228 206 230 247 146 135 371 291 292 0 1 0 907 252 265 249 350 425
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Golgi 2 2 0 1 6 1 2 1 3 2 1 20 5 4 12 25 31 1 0 1 2 1 2
Mitochondria 4 0 11 40 15 39 40 79 58 82 54 0 5 0 0 6 0 4 1 3 2 4 3
Nucleus 1 0 0 0 0 1 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 5 1 17 15 20 35 10 35 21 10 12 9 17 12 0 0 0 5 1 0 1 2 1
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 41 2 17 2 6 7 31 21 44 10 17 6 0 3 14 15 17 7 1 2 3 5 11
Unique Cell Count 905 261 273 210 251 291 336 350 411 278 240 417 355 347 46 68 67 974 267 284 270 391 473
Labelled Cell Count 983 283 329 287 325 362 392 470 514 366 308 506 448 423 52 83 78 974 267 284 270 391 473


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.6 29.7 14.6 18.2 16.6 13.9 13.8 12.8 13.4 12.5 12.5 24.5 24.6 22.5 36.8 35.5 37.1 25.3 25.0 25.5
Std Deviation (1e-4) 4.9 5.3 3.1 3.4 3.1 2.5 3.0 2.5 2.4 2.4 2.5 4.7 4.4 4.0 10.0 8.7 9.1 4.9 4.8 4.7
Intensity Change (Log2) 0.32 0.19 -0.07 -0.07 -0.19 -0.13 -0.22 -0.23 0.75 0.76 0.63 1.34 1.28 1.35 0.8 0.78 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 1.3 0.3 2.8 3.5 3.3 3.8 3.1 1.2 1.2 -0.1 0 0 0
Bud 0 -1.4 0 -0.7 0 0 -0.9 -0.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4.5 1.6 -0.8 0.5 1.3 2.2 3.4 1.3 4.0 6.7 6.2 0 5.8 5.3
Cytoplasm 0.5 1.7 1.8 3.1 2.2 3.4 3.6 4.3 -1.1 0.3 0.4 0 0 0
Endoplasmic Reticulum -2.5 -0.4 -2.9 -7.3 -6.3 -7.8 -9.0 -8.0 0.6 -1.9 -1.2 -12.7 -13.9 -14.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.7 0 0 0 0 11.8
Mitochondria 5.3 1.0 3.9 3.5 6.5 4.3 8.0 6.3 0 -2.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.4 0.8 2.4 -1.9 1.7 -0.6 -1.4 -0.6 -2.7 -0.8 -1.6 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 -2.1 -2.2 1.4 -0.1 2.0 -1.4 0.4 -3.4 -4.8 -3.8 0 4.0 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.2596 25.1018 24.7974 19.0763 21.763 24.7272 19.6048 22.3903 19.8102 20.9016 18.2723 21.2876 23.8156 26.9327 25.2773 24.5461 21.5176 25.0496
Actin 0.0079 0.0011 0.0105 0.0073 0.0047 0.0012 0.0277 0.0003 0.0049 0.0007 0.0024 0.0009 0.0312 0.0019 0.0143 0.0014 0.0176 0.0014
Bud 0.0003 0.0001 0.0002 0.0004 0.0004 0 0.0003 0 0.0004 0 0.0003 0 0.0004 0.0001 0.0003 0.0002 0.0008 0
Bud Neck 0.0008 0.0003 0.0006 0.002 0.0018 0.0009 0.0005 0.0002 0.0004 0.0002 0.0003 0.0012 0.0033 0.0005 0.0005 0.0001 0.004 0.001
Bud Periphery 0.0005 0.0002 0.0002 0.0008 0.0006 0.0002 0.0007 0.0001 0.0006 0 0.0007 0.0001 0.0006 0.0002 0.0003 0.0004 0.0015 0.0001
Bud Site 0.0025 0.0005 0.0009 0.0017 0.0073 0.0001 0.0009 0.0003 0.0008 0 0.0009 0.0001 0.0058 0.0026 0.0055 0.0005 0.001 0
Cell Periphery 0.0046 0.0089 0.0027 0.0035 0.0032 0.001 0.0062 0.0076 0.0048 0.001 0.003 0.0011 0.0023 0.0113 0.0052 0.0006 0.0146 0.0009
Cytoplasm 0.0099 0.0269 0.024 0.0148 0.0034 0.0057 0.0087 0.0131 0.012 0.0016 0.0014 0.0081 0.0155 0.033 0.0246 0.0063 0.0041 0.0049
Cytoplasmic Foci 0.0105 0.0004 0.0024 0.0044 0.004 0.0016 0.0095 0.0002 0.014 0.0004 0.0068 0.0014 0.0341 0.0003 0.0018 0.0026 0.0118 0
Eisosomes 0.0009 0.0001 0.0001 0.0003 0.0004 0 0.0017 0 0.0002 0 0.0006 0.0001 0.0002 0 0.0002 0.0001 0.0004 0
Endoplasmic Reticulum 0.7191 0.9276 0.9129 0.831 0.8935 0.9404 0.7878 0.9518 0.8665 0.9691 0.8251 0.9533 0.7372 0.9211 0.9275 0.8674 0.6946 0.948
Endosome 0.0154 0.0005 0.0102 0.0237 0.0188 0.0042 0.0275 0.0003 0.0215 0.0076 0.0617 0.0027 0.0363 0.0005 0.004 0.0467 0.0208 0.001
Golgi 0.0035 0.0003 0.0015 0.0065 0.0078 0.0024 0.009 0.0003 0.0094 0.0018 0.0169 0.0007 0.0105 0.0003 0.0015 0.0055 0.0347 0.0002
Lipid Particles 0.0135 0.0101 0.0027 0.0141 0.0118 0.002 0.0124 0.0116 0.017 0.0011 0.026 0.0018 0.0173 0.0131 0.0013 0.0082 0.0842 0.0002
Mitochondria 0.0052 0.0006 0.0037 0.0328 0.0074 0.0025 0.0152 0 0.0117 0.0001 0.0177 0.0055 0.0023 0 0.0014 0.0163 0.0472 0.0001
None 0.0131 0.0001 0.0004 0.0023 0.0016 0.0001 0.0041 0 0.0045 0.0002 0.0007 0.0003 0.0047 0 0.0033 0.0016 0.0003 0
Nuclear Periphery 0.1411 0.0139 0.0167 0.0276 0.0193 0.029 0.0589 0.0095 0.0115 0.0137 0.0133 0.0157 0.0532 0.011 0.0042 0.025 0.01 0.0355
Nucleolus 0.0046 0 0.0003 0.0023 0.0009 0 0.0006 0 0.0002 0 0.0007 0.0001 0.0008 0 0 0.0004 0.0001 0
Nucleus 0.0168 0.0009 0.0014 0.0056 0.0013 0.0024 0.0065 0.0007 0.0006 0.0003 0.0022 0.0009 0.0037 0.0006 0.0003 0.0028 0.0006 0.0022
Peroxisomes 0.0047 0 0.0012 0.0014 0.0018 0.0012 0.0022 0 0.0042 0 0.0017 0.0003 0.0213 0 0.0002 0.0014 0.0412 0
Punctate Nuclear 0.0101 0.0001 0.0005 0.0027 0.0004 0.0001 0.0059 0 0.0004 0.0001 0.0038 0.0028 0.0054 0.0001 0.0001 0.0009 0.0005 0
Vacuole 0.0103 0.0059 0.0042 0.0093 0.008 0.0031 0.0097 0.0036 0.0121 0.0017 0.0077 0.0023 0.0114 0.0032 0.0031 0.0068 0.008 0.0036
Vacuole Periphery 0.005 0.0014 0.0027 0.0056 0.0018 0.0018 0.004 0.0003 0.0024 0.0005 0.0061 0.0005 0.0024 0.0002 0.0003 0.0049 0.002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.7467 85.5394 67.441 83.0123 91.1094 80.6922 72.9749 90.2974 70.4637 82.35
Translational Efficiency 1.5856 1.6338 1.8199 1.1613 1.1856 1.3238 1.5551 0.9466 1.3708 1.2735

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Pkr1

Pkr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pkr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available