Standard name
Human Ortholog
Description GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 1.0 0.98 0.94 0.94 0.95 0.89 0.87 0.8 0.79 0.99 1.0 1.0 0.98 0.98 0.99 0.93 0.9 0.97 0.95 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 0.07 0.13 0.16 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 1 0 2 1 2 0 0 0 0 0 0 0 0 0 0 1 3 3
Bud 0 0 0 0 0 0 1 2 3 3 10 6 0 1 0 0 0 0 0 0 0 5 11 20
Bud Neck 0 0 0 0 2 1 2 3 8 3 1 1 0 0 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 14 6 3 2 2 1 1 2 3 6 0 4 2 5 3 9 0 0 0 0 0 0
Cytoplasm 137 156 122 355 507 171 385 479 511 617 354 396 393 764 934 241 223 188 135 156 131 446 547 699
Endoplasmic Reticulum 3 0 0 1 1 0 0 0 0 1 0 1 0 0 0 7 11 9 0 0 0 2 3 4
Endosome 0 0 0 0 2 0 1 3 6 8 4 3 1 1 1 1 1 0 2 0 0 2 1 3
Golgi 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 2 2
Mitochondria 3 2 0 0 5 26 16 33 74 114 123 125 1 0 2 2 3 2 2 1 0 3 6 4
Nucleus 0 1 1 0 0 2 1 2 3 5 3 3 0 0 3 2 2 0 0 1 0 0 0 2
Nuclear Periphery 0 2 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 2 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 1 0 6 4 4 10 4 5 0 0 0 0 0 0 0 0 0 0 7 4
Vac/Vac Membrane 0 0 0 2 7 0 19 9 19 21 11 17 0 1 1 4 2 0 1 9 0 0 2 3
Unique Cell Count 141 158 124 356 518 181 411 504 575 708 440 500 395 765 938 247 227 190 146 174 135 469 596 760
Labelled Cell Count 144 162 138 364 531 202 435 537 631 789 516 567 395 772 945 264 245 209 146 174 135 469 596 760


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.1 6.0 5.9 6.3 4.0 5.3 5.0 4.5 4.6 4.4 4.6 5.8 5.8 6.0 7.7 7.4 7.8 5.2 5.2 5.5
Std Deviation (1e-4) 0.6 0.6 0.7 1.4 1.7 1.1 1.8 1.1 1.4 1.4 1.1 1.5 1.1 1.2 1.6 1.3 0.9 1.2 1.1 1.5 1.6
Intensity Change (Log2) – – – -0.01 0.08 -0.57 -0.18 -0.25 -0.4 -0.37 -0.45 -0.38 -0.05 -0.03 0.01 0.36 0.3 0.38 -0.21 -0.19 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -4.6 0 0 0 0 0 0 0 0 0 0 0 -3.8 -4.2 -2.2
Cytoplasm 1.6 -0.4 -1.7 -2.1 -1.6 -3.3 -3.7 -4.9 -5.1 1.2 2.6 1.7 -0.5 -0.1 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7717 0.2122 -0.1421 -0.1826 0.4578 -0.2919 -0.1507 0.4813 0.2828 0.0655 0.461 0.074 1.987 1.768 2.0423 1.9328 1.78 2.0813
Actin 0.0565 0.0024 0.0128 0.0532 0.0208 0.0043 0.0332 0.0008 0.0126 0.0087 0.0496 0.0014 0.0675 0.0003 0.0007 0.0001 0.0001 0.0001
Bud 0.005 0.0087 0.0061 0.0027 0.0095 0.0011 0.0008 0.0008 0.0005 0.0004 0.0045 0.0002 0.0004 0.0001 0.0002 0.0001 0 0
Bud Neck 0.0071 0.0007 0.0068 0.0052 0.0258 0.001 0.0028 0.0002 0.0091 0.0047 0.0017 0.0019 0.0039 0 0.0003 0 0 0.0001
Bud Periphery 0.0114 0.0066 0.0083 0.0072 0.0438 0.0035 0.0016 0.0009 0.0006 0.0005 0.0053 0.0003 0.0007 0.0002 0.0002 0 0 0
Bud Site 0.0147 0.0107 0.015 0.0046 0.0597 0.0009 0.0047 0.0058 0.0023 0.003 0.0439 0.0001 0.0027 0.0001 0.0013 0 0 0
Cell Periphery 0.0009 0.0006 0.0005 0.0004 0.0014 0.0001 0.0004 0.0002 0.0002 0.0002 0.0005 0 0.0003 0.0001 0.0001 0 0 0
Cytoplasm 0.1227 0.2558 0.2309 0.1617 0.1831 0.4125 0.3089 0.3979 0.401 0.5208 0.4274 0.5235 0.1234 0.1675 0.2879 0.2345 0.3561 0.4017
Cytoplasmic Foci 0.0192 0.0112 0.017 0.0103 0.0314 0.0189 0.0143 0.0067 0.0096 0.0136 0.0504 0.0126 0.0231 0.0035 0.0104 0.0043 0.005 0.0028
Eisosomes 0.0006 0.0002 0.0002 0.0011 0.0004 0.0001 0.0004 0.0001 0.0002 0.0001 0.0005 0 0.0007 0.0003 0.0001 0 0 0
Endoplasmic Reticulum 0.008 0.0024 0.0022 0.0082 0.0028 0.003 0.0067 0.0023 0.0025 0.003 0.0017 0.0021 0.0018 0.0008 0.0011 0.0026 0.0014 0.0004
Endosome 0.0222 0.0016 0.0049 0.0123 0.0213 0.0103 0.0233 0.002 0.0092 0.0131 0.028 0.0082 0.0167 0.0006 0.0021 0.0011 0.0014 0.0005
Golgi 0.0059 0.0003 0.0013 0.0019 0.0059 0.0015 0.0047 0.0002 0.0031 0.0038 0.006 0.0002 0.0087 0.0001 0.0004 0.0001 0.0001 0
Lipid Particles 0.0123 0.0008 0.0039 0.0021 0.0111 0.0018 0.006 0.0003 0.0038 0.007 0.0026 0.0002 0.0234 0.0003 0.0005 0.0003 0 0
Mitochondria 0.0077 0.0015 0.0011 0.0028 0.0742 0.0023 0.0028 0.0003 0.0025 0.0007 0.0101 0.0002 0.0031 0.0004 0.0003 0.0002 0.0001 0
None 0.607 0.6861 0.67 0.6825 0.3993 0.5169 0.5534 0.5776 0.5349 0.4108 0.3528 0.4453 0.6618 0.8243 0.6908 0.7545 0.6346 0.5935
Nuclear Periphery 0.0378 0.0008 0.0067 0.0159 0.011 0.0094 0.0183 0.0007 0.0009 0.0013 0.0013 0.0007 0.0039 0.0002 0.0003 0.0005 0.0002 0.0001
Nucleolus 0.0051 0.0009 0.0005 0.0017 0.0075 0.0008 0.0006 0.0001 0.0002 0.0005 0.0008 0 0.0029 0.0001 0.0002 0.0001 0 0
Nucleus 0.0198 0.0038 0.002 0.0073 0.018 0.0034 0.0036 0.0011 0.0009 0.0012 0.0021 0.0011 0.0043 0.0003 0.0006 0.0005 0.0003 0.0003
Peroxisomes 0.0107 0.001 0.0032 0.0015 0.0141 0.0019 0.0031 0.0001 0.0024 0.0017 0.0027 0.0001 0.0318 0.0003 0.0008 0.0001 0 0
Punctate Nuclear 0.0156 0.0017 0.0046 0.0148 0.0495 0.0039 0.0059 0.0006 0.0014 0.0021 0.003 0.0007 0.0172 0.0002 0.001 0.0002 0.0001 0.0001
Vacuole 0.0063 0.0019 0.0015 0.0017 0.0083 0.0015 0.0028 0.0013 0.0015 0.0024 0.004 0.0008 0.0013 0.0003 0.0005 0.0006 0.0003 0.0002
Vacuole Periphery 0.0034 0.0002 0.0003 0.0008 0.0011 0.0011 0.0016 0.0001 0.0005 0.0004 0.001 0.0002 0.0007 0 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5782 11.9872 6.3536 9.7238 6.2093 3.8291 5.1944 9.349 9.7602 8.3513
Translational Efficiency 0.7733 0.7633 1.2285 0.8485 1.3727 1.7995 1.8016 0.8853 0.8614 0.8075

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cin4

Cin4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cin4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available